breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 1,843,140 G→A Q566* (CAG→TAG)  gacS ← sensor protein GacS
RA 1,852,005 G→A R350H (CGT→CAT)  relA → ATP:GTP 3'‑pyrophosphotransferase
RA 4,741,280 C→T intergenic (+471/+281) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,283 C→G intergenic (+474/+278) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,285 2 bp→CT intergenic (+476/+275) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 5,610,048 C→A S173I (AGC→ATC)  PP_4929 ← LysR family transcriptional regulator
RA 5,912,914 G→A E43K (GAG→AAG)  spuI → glutamylpolyamine synthetase
RA 5,914,046 +A coding (1259/1377 nt) spuI → glutamylpolyamine synthetase
RA 6,180,730 C→G W258S (TGG→TCG)  atpB ← ATP synthase subunit a

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947 171382–176286 176286 1–4905 9 [8] [8] 9 PP_16SA–[PP_5SA] PP_16SA,PP_23SA,[PP_5SA]
* * ÷ NC_002947 176674–181686 181686 1–5013 9 [8] [8] 9 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947 1131767 1131831 65 9 [7] [7] 10 PP_0992 hypothetical protein
* * ÷ NC_002947 4808205 4808218 14 21 [0] [0] 27 PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 = 9037433 (0.940)10 (0.320) 9/240 2.3 24.4% intergenic (+49/+73) PP_0079/PP_0080 transcriptional regulator/hypothetical protein
?NC_002947 = 90397 33 (1.060)intergenic (+72/+50) PP_0079/PP_0080 transcriptional regulator/hypothetical protein
* ? NC_002947 = 176399NA (NA)22 (0.650)
+CGAAA
14/262 1.6 30.5% intergenic (+43/‑420) PP_5SA/PP_16SB 5S ribosomal RNA/16S ribosomal RNA
?NC_002947 = 182013 52 (1.470)intergenic (+2/+63) PP_5SB/PP_0160 5S ribosomal RNA/ferrioxamine receptor
* ? NC_002947 413997 =18 (0.510)12 (0.430) 10/216 1.8 45.0% intergenic (+14/‑189) glnE/waaF glutamate‑ammonia‑ligase adenylyltransferase/ADP‑heptose:LPS heptosyltransferase II
?NC_002947 414083 = 15 (0.540)intergenic (+100/‑103) glnE/waaF glutamate‑ammonia‑ligase adenylyltransferase/ADP‑heptose:LPS heptosyltransferase II
* ? NC_002947 = 41402418 (0.510)9 (0.320) 8/216 2.2 38.1% intergenic (+41/‑162) glnE/waaF glutamate‑ammonia‑ligase adenylyltransferase/ADP‑heptose:LPS heptosyltransferase II
?NC_002947 = 414054 15 (0.540)intergenic (+71/‑132) glnE/waaF glutamate‑ammonia‑ligase adenylyltransferase/ADP‑heptose:LPS heptosyltransferase II
* ? NC_002947 = 65278523 (0.650)15 (0.500) 13/232 1.4 60.5% coding (1961/1962 nt) ctpL methyl‑accepting chemotaxis protein CtpL
?NC_002947 = 4808204 0 (0.000)intergenic (‑268/‑287) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* ? NC_002947 1296580 =NA (NA)13 (0.390) 12/254 1.8 NA intergenic (‑327/‑239) nhaA‑I/PP_1133 Na(+)/H(+) antiporter NhaA/transposase
?NC_002947 1296616 = NA (NA)intergenic (‑363/‑203) nhaA‑I/PP_1133 Na(+)/H(+) antiporter NhaA/transposase
* ? NC_002947 1731205 =28 (0.790)7 (0.250) 7/216 2.5 26.8% intergenic (‑76/+115) dapE/PP_1526 succinyl‑diaminopimelate desuccinylase/beta‑(1‑3)‑glucosyl transferase
?NC_002947 1731297 = 16 (0.570)intergenic (‑168/+23) dapE/PP_1526 succinyl‑diaminopimelate desuccinylase/beta‑(1‑3)‑glucosyl transferase
* ? NC_002947 1967188 =7 (0.200)9 (0.340) 7/206 2.4 52.5% intergenic (+15/+104) PP_1762/yciK hypothetical protein/putative oxidoreductase
?NC_002947 1967284 = 11 (0.410)intergenic (+111/+8) PP_1762/yciK hypothetical protein/putative oxidoreductase
* ? NC_002947 = 19672207 (0.200)8 (0.300) 6/206 2.7 49.5% intergenic (+47/+72) PP_1762/yciK hypothetical protein/putative oxidoreductase
?NC_002947 = 1967250 11 (0.410)intergenic (+77/+42) PP_1762/yciK hypothetical protein/putative oxidoreductase
* ? NC_002947 4074203 =NA (NA)15 (0.440) 9/262 2.6 69.0% coding (1256/1329 nt) PP_3586 transposase
?NC_002947 5545215 = 7 (0.200)intergenic (‑307/+570) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
* ? NC_002947 4340085 =13 (0.370)10 (0.380) 8/204 2.1 65.0% intergenic (+26/‑305) ribAB‑II/PP_3814 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein
?NC_002947 4340169 = 1 (0.040)intergenic (+110/‑221) ribAB‑II/PP_3814 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein
* ? NC_002947 = 458610016 (0.450)15 (0.590) 10/196 1.5 52.1% intergenic (+139/+78) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
?NC_002947 = 4586128 16 (0.630)intergenic (+167/+50) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
* ? NC_002947 4808219 =0 (0.000)18 (0.690) 16/202 0.7 57.1% intergenic (‑283/‑272) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947 = 5988991 27 (1.030)intergenic (+229/‑317) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947 = 489644329 (0.820)14 (0.470) 9/230 2.2 35.7% intergenic (+31/‑177) PP_4304/sbp‑I VIC family cation transporter/sulfate ABC transporter substrate‑binding protein
?NC_002947 = 4896490 26 (0.870)intergenic (+78/‑130) PP_4304/sbp‑I VIC family cation transporter/sulfate ABC transporter substrate‑binding protein
* ? NC_002947 = 590382524 (0.680)11 (0.340) 8/252 2.7 34.2% intergenic (+25/+82) PP_5176/spuH hypothetical protein/spermidine/putrescine ABC transporter permease
?NC_002947 = 5903887 20 (0.610)intergenic (+87/+20) PP_5176/spuH hypothetical protein/spermidine/putrescine ABC transporter permease
* ? NC_002947 5903827 =24 (0.680)11 (0.350) 7/240 2.8 34.8% intergenic (+27/+80) PP_5176/spuH hypothetical protein/spermidine/putrescine ABC transporter permease
?NC_002947 5903887 = 20 (0.640)intergenic (+87/+20) PP_5176/spuH hypothetical protein/spermidine/putrescine ABC transporter permease
* ? NC_002947 = 591424017 (0.480)7 (0.250) 7/216 2.5 39.4% intergenic (+76/+77) spuI/argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase
?NC_002947 = 5914280 8 (0.290)intergenic (+116/+37) spuI/argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase
* ? NC_002947 = 600663014 (0.400)9 (0.350) 7/200 2.3 40.6% intergenic (+42/+57) ydcJ/PP_5261 metalloprotein/LysR family transcriptional regulator
?NC_002947 = 6006642 16 (0.620)intergenic (+54/+45) ydcJ/PP_5261 metalloprotein/LysR family transcriptional regulator
* ? NC_002947 = 606050029 (0.820)7 (0.220) 7/244 2.9 20.6% intergenic (+80/+101) PP_5311/PP_5312 signal transduction protein/hypothetical protein
?NC_002947 = 6060559 28 (0.880)intergenic (+139/+42) PP_5311/PP_5312 signal transduction protein/hypothetical protein