breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 66 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_021214358,5110GA38.2% 217.7 / 97.1 166intergenic (‑93/‑92)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein
*NC_021214358,5240TA35.3% 284.7 / 58.2 150intergenic (‑106/‑79)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein
*NC_0212141,239,6280TG31.5% 329.5 / 47.5 185C97G (TGT→GGT) TMARI_RS06210oxidoreductase
*NC_021214358,5450GT28.7% 382.6 / 63.4 157intergenic (‑127/‑58)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein
*NC_021214358,5600TA28.1% 391.0 / 43.1 160intergenic (‑142/‑43)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein
*NC_021214693,6230GT27.2% 288.2 / 68.9 152A237A (GCC→GCATMARI_RS03400cysteine synthase A
*NC_021214724,1700AT26.8% 472.8 / 76.3 214I257K (ATA→AAA) TMARI_RS03585chemotaxis protein CheA
*NC_0212141,869,4460GT26.5% 365.6 / 41.6 153intergenic (+58/‑53)TMARI_RS09635/TMARI_RS09660bifunctional UDP‑sugar hydrolase/5'‑nucleotidase/hypothetical protein
*NC_021214622,2530GA26.4% 391.7 / 61.3 201A48A (GCG→GCATMARI_RS03015rod shape‑determining protein
*NC_0212141,869,4340TC26.4% 317.9 / 17.7 150intergenic (+46/‑65)TMARI_RS09635/TMARI_RS09660bifunctional UDP‑sugar hydrolase/5'‑nucleotidase/hypothetical protein
*NC_021214358,5860GT26.0% 487.7 / 52.0 169intergenic (‑168/‑17)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein
*NC_021214232,2000GA25.9% 367.5 / 54.0 185R259Q (CGA→CAA) TMARI_RS01120glycine‑‑tRNA ligase subunit beta
*NC_0212141,762,7900AC25.9% 488.0 / 23.8 234G39G (GGT→GGGTMARI_RS09145hypothetical protein
*NC_021214602,9200TG25.8% 401.6 / 20.5 187V382G (GTT→GGT) 
L17V (TTG→GTG) 
TMARI_RS02930
TMARI_RS02935
DegT/DnrJ/EryC1/StrS aminotransferase family protein
apolipoprotein N‑acyltransferase
*NC_0212141,739,8640TG25.6% 373.1 / 25.1 162G390G (GGT→GGGTMARI_RS09025transketolase
*NC_02121426,4680CA25.5% 533.8 / 53.0 192E32* (GAG→TAG) TMARI_RS00115ABC transporter ATP‑binding protein
*NC_021214622,2720AC25.4% 428.9 / 11.6 190T55P (ACT→CCT) TMARI_RS03015rod shape‑determining protein
*NC_021214817,1080CT25.2% 349.1 / 41.3 148F402F (TTC→TTTTMARI_RS04060hypothetical protein
*NC_021214622,2670GA24.6% 409.7 / 62.2 199G53E (GGA→GAA) TMARI_RS03015rod shape‑determining protein
*NC_0212141,869,4240CG24.2% 141.5 / 18.7 149intergenic (+36/‑75)TMARI_RS09635/TMARI_RS09660bifunctional UDP‑sugar hydrolase/5'‑nucleotidase/hypothetical protein

Marginal new junction evidence (lowest skew 10 of 34 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_021214 157995 =251 (0.880)9 (0.030) 6/430 23.1 3.6% coding (478/648 nt) TMARI_RS00740 lactate utilization protein
?NC_021214 158204 = 246 (0.900)coding (1857/1872 nt) TMARI_RS00745 DUF4914 domain‑containing protein
* ? NC_021214 = 591522246 (0.860)6 (0.020) 6/434 23.2 2.5% coding (471/549 nt) TMARI_RS02865 DNA polymerase
?NC_021214 = 591525 234 (0.850)coding (474/549 nt) TMARI_RS02865 DNA polymerase
* ? NC_021214 = 360433334 (1.170)6 (0.020) 5/428 23.8 1.9% coding (756/1242 nt) TMARI_RS01750 MFS transporter
?NC_021214 = 360642 317 (1.160)coding (965/1242 nt) TMARI_RS01750 MFS transporter
* ? NC_021214 1440262 =285 (1.000)6 (0.020) 5/430 23.9 1.9% coding (1298/1881 nt) TMARI_RS07270 NADH dehydrogenase
?NC_021214 1440572 = 331 (1.210)coding (1608/1881 nt) TMARI_RS07270 NADH dehydrogenase
* ? NC_021214 1327950 =357 (1.250)8 (0.030) 5/430 23.9 2.4% coding (408/762 nt) TMARI_RS06635 ABC transporter permease
?NC_021214 1328134 = 319 (1.170)coding (592/762 nt) TMARI_RS06635 ABC transporter permease
* ? NC_021214 = 491988296 (1.030)6 (0.020) 5/434 24.0 1.9% coding (711/936 nt) TMARI_RS02360 RluA family pseudouridine synthase
?NC_021214 = 492118 318 (1.150)coding (581/936 nt) TMARI_RS02360 RluA family pseudouridine synthase
* ? NC_021214 63321 =274 (0.960)5 (0.020) 5/436 24.1 1.8% coding (3179/3180 nt) TMARI_RS00285 endo‑1,4‑beta‑xylanase A
?NC_021214 63342 = 279 (1.010)intergenic (+20/‑36) TMARI_RS00285/TMARI_RS00290 endo‑1,4‑beta‑xylanase A/carbohydrate‑binding protein
* ? NC_021214 1071755 =291 (1.020)6 (0.020) 5/440 24.2 1.9% coding (662/1527 nt) TMARI_RS05395 FMN‑binding glutamate synthase family protein
?NC_021214 1071819 = 339 (1.210)coding (726/1527 nt) TMARI_RS05395 FMN‑binding glutamate synthase family protein
* ? NC_021214 228871 =275 (0.960)5 (0.020) 5/440 24.2 1.8% coding (104/1425 nt) TMARI_RS01105 glycine dehydrogenase
?NC_021214 229168 = 292 (1.040)coding (401/1425 nt) TMARI_RS01105 glycine dehydrogenase
* ? NC_021214 = 1501608304 (1.060)5 (0.020) 4/426 24.6 1.7% coding (553/2088 nt) TMARI_RS07670 elongation factor G
?NC_021214 = 1501747 288 (1.060)coding (414/2088 nt) TMARI_RS07670 elongation factor G