Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F999 I0 R1
|
73 |
59.3 |
4372352 |
98.6% |
4311139 |
49.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,438,124 |
T→C |
W140R (TGG→CGG) |
ytfI → |
protein YtfI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,438,124 | 0 | T | C | 100.0%
| 231.6
/ NA
| 57 | W140R (TGG→CGG) | ytfI | protein YtfI |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/23); total (34/23) |
TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA > NC_000913/4438077‑4438169
|
tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg > 1:1878306/1‑50 (MQ=255)
aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa > 1:4105369/1‑50 (MQ=255)
aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat < 1:3819536/50‑1 (MQ=255)
aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt > 1:4065918/1‑50 (MQ=255)
ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc > 1:144335/1‑50 (MQ=255)
cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt < 1:3178049/50‑1 (MQ=255)
cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt < 1:1102109/50‑1 (MQ=255)
gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa < 1:2089542/50‑1 (MQ=255)
gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa > 1:1930084/1‑50 (MQ=255)
gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa > 1:4286861/1‑50 (MQ=255)
tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc < 1:1503037/50‑1 (MQ=255)
tGAATTTCCGTAAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc < 1:2674905/50‑1 (MQ=255)
gAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa > 1:1073104/1‑48 (MQ=255)
aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa < 1:2784136/49‑1 (MQ=255)
aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg > 1:302477/1‑49 (MQ=255)
aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa < 1:1274553/50‑1 (MQ=255)
ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa > 1:1234814/1‑49 (MQ=255)
ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa > 1:3406924/1‑49 (MQ=255)
cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa > 1:1569714/1‑50 (MQ=255)
cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa < 1:2569384/50‑1 (MQ=255)
cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa > 1:2378719/1‑50 (MQ=255)
ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa < 1:4204218/50‑1 (MQ=255)
aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt > 1:4215372/1‑50 (MQ=255)
aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt > 1:180745/1‑50 (MQ=255)
gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc > 1:1217731/1‑50 (MQ=255)
gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc > 1:1145982/1‑50 (MQ=255)
gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc < 1:1839430/50‑1 (MQ=255)
aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt > 1:1812173/1‑50 (MQ=255)
cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa > 1:1077687/1‑50 (MQ=255)
cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa > 1:458251/1‑50 (MQ=255)
aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa > 1:4125458/1‑49 (MQ=255)
aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc < 1:846978/50‑1 (MQ=255)
aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa < 1:629207/50‑1 (MQ=255)
aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt < 1:3853703/50‑1 (MQ=255)
aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa < 1:1283302/50‑1 (MQ=255)
aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa > 1:3097145/1‑50 (MQ=255)
aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa > 1:3261817/1‑50 (MQ=255)
aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa > 1:4078218/1‑50 (MQ=255)
aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa > 1:3455044/1‑50 (MQ=255)
aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc > 1:1331241/1‑50 (MQ=255)
aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc < 1:1572429/50‑1 (MQ=255)
aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc > 1:822815/1‑50 (MQ=255)
aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc < 1:1886912/50‑1 (MQ=255)
gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc > 1:1251911/1‑49 (MQ=255)
aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg > 1:2502467/1‑49 (MQ=255)
aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg > 1:2465745/1‑49 (MQ=255)
cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa < 1:2302333/50‑1 (MQ=255)
cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa < 1:2429345/50‑1 (MQ=255)
ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg > 1:1863031/1‑50 (MQ=255)
ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg < 1:310852/50‑1 (MQ=255)
tGTATGCCCGGGATATTCTACAGAAAAAACCGTCTGACATACCGCAAAg > 1:4074773/1‑49 (MQ=255)
tGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg > 1:1000575/1‑49 (MQ=255)
gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg < 1:4211794/49‑1 (MQ=255)
aTGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt > 1:3866775/1‑49 (MQ=255)
tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt < 1:1498815/49‑1 (MQ=255)
tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt < 1:1677301/49‑1 (MQ=255)
gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa > 1:619932/1‑49 (MQ=255)
|
TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA > NC_000913/4438077‑4438169
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
