Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F999 I0 R1
|
73 |
75.1 |
5586924 |
96.8% |
5408142 |
49.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,438,124 |
T→C |
W140R (TGG→CGG) |
ytfI → |
protein YtfI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,438,124 | 0 | T | C | 100.0%
| 358.8
/ NA
| 88 | W140R (TGG→CGG) | ytfI | protein YtfI |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/54); total (34/54) |
TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA > NC_000913/4438077‑4438169
|
tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg > 1:1165284/1‑50 (MQ=255)
cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg < 1:955755/49‑1 (MQ=255)
aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCggg < 1:5085764/49‑1 (MQ=255)
aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa < 1:3696672/50‑1 (MQ=255)
aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat < 1:3365659/50‑1 (MQ=255)
aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat < 1:3886843/50‑1 (MQ=255)
aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat < 1:4604529/50‑1 (MQ=255)
aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa > 1:358967/1‑49 (MQ=255)
aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATACCCGGGa > 1:2878117/1‑49 (MQ=38)
aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat < 1:1168884/50‑1 (MQ=255)
aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat < 1:2003937/50‑1 (MQ=255)
ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc > 1:3405647/1‑50 (MQ=255)
ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc < 1:2093288/50‑1 (MQ=255)
cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc > 1:1447812/1‑49 (MQ=255)
cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt < 1:266805/50‑1 (MQ=255)
cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt < 1:4812813/50‑1 (MQ=255)
cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt < 1:4917017/50‑1 (MQ=255)
gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa < 1:212131/50‑1 (MQ=255)
gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa < 1:4108434/50‑1 (MQ=255)
gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa > 1:3886949/1‑50 (MQ=255)
gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa < 1:929390/50‑1 (MQ=255)
gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa < 1:966930/50‑1 (MQ=255)
gTGAATCTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa < 1:5575838/50‑1 (MQ=255)
tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc < 1:839496/50‑1 (MQ=255)
gAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa > 1:3212088/1‑48 (MQ=255)
gAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa > 1:3196369/1‑48 (MQ=255)
aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa < 1:5018938/49‑1 (MQ=255)
aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa < 1:856546/49‑1 (MQ=255)
aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg < 1:1950332/50‑1 (MQ=255)
aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg > 1:1109143/1‑49 (MQ=255)
aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg > 1:2348604/1‑49 (MQ=255)
tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa < 1:454743/50‑1 (MQ=255)
tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa < 1:375558/50‑1 (MQ=255)
ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa > 1:88978/1‑49 (MQ=255)
ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa > 1:5286084/1‑49 (MQ=255)
tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa < 1:1662726/49‑1 (MQ=255)
tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa > 1:1817906/1‑49 (MQ=255)
ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa < 1:134942/49‑1 (MQ=255)
ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa > 1:1669091/1‑49 (MQ=255)
ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa > 1:3034431/1‑49 (MQ=255)
gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa > 1:2632254/1‑49 (MQ=255)
gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa > 1:2271996/1‑49 (MQ=255)
ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa < 1:1766557/50‑1 (MQ=255)
tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg > 1:2856605/1‑50 (MQ=255)
tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg > 1:4707347/1‑50 (MQ=255)
aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg < 1:3754821/49‑1 (MQ=255)
gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt < 1:5254433/49‑1 (MQ=255)
gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc < 1:153816/50‑1 (MQ=255)
gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc < 1:1651065/50‑1 (MQ=255)
aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt < 1:955650/50‑1 (MQ=255)
aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt > 1:1923577/1‑50 (MQ=255)
aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt > 1:1892847/1‑49 (MQ=255)
aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg < 1:3245139/50‑1 (MQ=255)
cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa > 1:1843485/1‑50 (MQ=255)
aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa > 1:4093634/1‑49 (MQ=255)
aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa > 1:1930732/1‑49 (MQ=255)
aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc < 1:1176388/50‑1 (MQ=255)
aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc < 1:3073929/50‑1 (MQ=255)
aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa < 1:5234980/50‑1 (MQ=255)
aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa > 1:2516429/1‑50 (MQ=255)
aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa < 1:3641876/50‑1 (MQ=255)
aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt < 1:4247239/50‑1 (MQ=255)
aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt < 1:3950149/50‑1 (MQ=255)
aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa < 1:2372398/50‑1 (MQ=255)
aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa < 1:135188/50‑1 (MQ=255)
aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa < 1:2979636/50‑1 (MQ=255)
cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc < 1:3675121/50‑1 (MQ=255)
cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc < 1:982342/50‑1 (MQ=255)
aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc < 1:1696076/50‑1 (MQ=255)
aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc < 1:677859/50‑1 (MQ=255)
gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc > 1:5252901/1‑49 (MQ=255)
gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg < 1:973377/50‑1 (MQ=255)
cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa < 1:2604288/50‑1 (MQ=255)
cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGa > 1:818230/1‑49 (MQ=255)
gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa < 1:5559147/50‑1 (MQ=255)
aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa < 1:2209564/50‑1 (MQ=255)
aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa > 1:705624/1‑48 (MQ=255)
ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg > 1:4685112/1‑50 (MQ=255)
ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg < 1:3658777/50‑1 (MQ=255)
ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg > 1:5096557/1‑50 (MQ=255)
tGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg > 1:1031787/1‑49 (MQ=255)
tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa < 1:2222409/49‑1 (MQ=255)
tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa < 1:914824/49‑1 (MQ=255)
tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa < 1:4295419/49‑1 (MQ=255)
tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt < 1:5550522/49‑1 (MQ=255)
tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt > 1:19730/1‑49 (MQ=255)
gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa > 1:78411/1‑49 (MQ=255)
gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa > 1:2979468/1‑49 (MQ=255)
|
TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA > NC_000913/4438077‑4438169
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
