Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F999 I0 R1
|
76 |
68.2 |
5140122 |
96.8% |
4975638 |
49.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,438,124 |
T→C |
W140R (TGG→CGG) |
ytfI → |
protein YtfI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,438,124 | 0 | T | C | 100.0%
| 216.8
/ NA
| 55 | W140R (TGG→CGG) | ytfI | protein YtfI |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/28); total (27/28) |
TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT > NC_000913/4438077‑4438168
|
tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg > 1:2100437/1‑50 (MQ=255)
cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg < 1:4368158/49‑1 (MQ=255)
cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg < 1:1918950/49‑1 (MQ=255)
aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa > 1:4415709/1‑50 (MQ=255)
aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa > 1:1466613/1‑50 (MQ=255)
aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat < 1:2157571/50‑1 (MQ=255)
aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa > 1:3591161/1‑49 (MQ=255)
aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat > 1:377120/1‑50 (MQ=255)
aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat < 1:1944237/50‑1 (MQ=255)
aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat < 1:644470/50‑1 (MQ=255)
aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt < 1:706550/50‑1 (MQ=255)
aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt > 1:632179/1‑50 (MQ=255)
cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt < 1:971915/50‑1 (MQ=255)
cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt < 1:563954/50‑1 (MQ=255)
tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa > 1:2273915/1‑49 (MQ=255)
aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg > 1:283111/1‑49 (MQ=255)
ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa > 1:2440825/1‑49 (MQ=255)
tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa < 1:3021972/49‑1 (MQ=255)
ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa < 1:2644845/49‑1 (MQ=255)
ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa > 1:3050323/1‑49 (MQ=255)
cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa < 1:4902893/50‑1 (MQ=255)
gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc < 1:4861081/50‑1 (MQ=255)
tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg > 1:2375605/1‑50 (MQ=255)
tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg > 1:3476026/1‑50 (MQ=255)
aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg < 1:1021809/49‑1 (MQ=255)
gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt < 1:2438915/49‑1 (MQ=255)
gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt < 1:913941/49‑1 (MQ=255)
gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt < 1:4351562/49‑1 (MQ=255)
gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc < 1:3473762/50‑1 (MQ=255)
aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAGAAACAGTCt < 1:1147446/50‑1 (MQ=255)
aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt < 1:2723104/50‑1 (MQ=255)
aCAAACCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg < 1:2868601/50‑1 (MQ=255)
aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg < 1:3552944/50‑1 (MQ=255)
aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg < 1:4632990/50‑1 (MQ=255)
aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa > 1:1435412/1‑49 (MQ=255)
aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa > 1:4056719/1‑49 (MQ=255)
aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAGAACAGTCTGACa < 1:3580132/50‑1 (MQ=255)
aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa > 1:3669098/1‑50 (MQ=255)
aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa > 1:4293620/1‑50 (MQ=255)
aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa > 1:1878619/1‑50 (MQ=255)
aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa > 1:3726388/1‑49 (MQ=255)
aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa > 1:2907806/1‑49 (MQ=255)
aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa < 1:385754/50‑1 (MQ=255)
aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc > 1:3152393/1‑50 (MQ=255)
gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg < 1:1493345/50‑1 (MQ=255)
aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg > 1:3452366/1‑49 (MQ=255)
gATGGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa < 1:4902863/50‑1 (MQ=255)
aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa > 1:3001344/1‑48 (MQ=255)
aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa > 1:1672235/1‑48 (MQ=255)
ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg < 1:1105801/50‑1 (MQ=255)
tGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg > 1:1074137/1‑49 (MQ=255)
tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa < 1:2553646/49‑1 (MQ=255)
aTGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt > 1:4873004/1‑49 (MQ=255)
aTGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt > 1:4634488/1‑49 (MQ=255)
tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt > 1:3742797/1‑49 (MQ=255)
|
TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT > NC_000913/4438077‑4438168
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
