Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F999 I0 R1	108	30.5	2257647	98.2%	2217009	49.6

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	78.1 / NA	21	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/15); total (6/15)

AAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT  >  NC_000913/4438079‑4438168

aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCggg                                           <  1:2202292/49‑1 (MQ=255)

aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                          <  1:1908941/50‑1 (MQ=255)

 aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                         <  1:401915/50‑1 (MQ=255)

 aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                         <  1:1970662/50‑1 (MQ=255)

     ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                     >  1:319351/1‑50 (MQ=255)

     ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                     <  1:2188700/50‑1 (MQ=255)

          aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                <  1:2067344/50‑1 (MQ=255)

            tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                              <  1:1423913/50‑1 (MQ=255)

              tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa                             >  1:1753197/1‑49 (MQ=255)

               ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                            <  1:1542576/49‑1 (MQ=255)

                 gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:1541806/1‑49 (MQ=255)

                   tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                       >  1:453075/1‑50 (MQ=255)

                    aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                      >  1:1041119/1‑50 (MQ=255)

                       aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg                   <  1:2114972/50‑1 (MQ=255)

                          aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                <  1:1103107/50‑1 (MQ=255)

                             cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc             <  1:1720043/50‑1 (MQ=255)

                              aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc            <  1:1892494/50‑1 (MQ=255)

                               gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg           <  1:925318/50‑1 (MQ=255)

                                    ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg      <  1:316743/50‑1 (MQ=255)

                                     tGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg      >  1:1206593/1‑49 (MQ=255)

                                         tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt  <  1:1414427/49‑1 (MQ=255)

AAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT  >  NC_000913/4438079‑4438168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A