Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F13 I0 R1 159 9491.7 7979620 98.5% 7859925 135.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,367 T→G 14.4% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3670TG14.4% 244.5 / 12.4 144F225V (TTT→GTT) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (25/95);  new base G (22/2);  total (47/97)
Fisher's exact test for biased strand distribution p-value = 5.28e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

ACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTC  >  NZ_CP022686/3734233‑3734503
                                                                                                                                       |                                                                                                                                         
accacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGATGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGC‑GGTTggg                                                                                                                                      >  7:244941/1‑139 (MQ=255)
 ccacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGgcc                                                                                                                                     <  8:827734/140‑1 (MQ=255)
  cacaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGC‑GGTTGggcg                                                                                                                                    >  7:266190/1‑140 (MQ=255)
  cacaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTTgg                                                                                                                                       >  3:453330/1‑138 (MQ=255)
  cacaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTggg                                                                                                                                      >  4:280352/1‑137 (MQ=255)
   acaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTGgccgc                                                                                                                                   >  8:549147/1‑140 (MQ=255)
    caGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTGggcg                                                                                                                                    >  2:708733/1‑138 (MQ=255)
       cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCCGGC‑GGTTGGgcgcccgc                                                                                                                               >  6:812010/1‑140 (MQ=255)
       cGGCCCGCTCAGTATGGCGGGAGATTTCAATGCCTGGAGCCGAAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGCTCAGCGCCGTCCGTC‑CTTTGGccgccc                                                                                                                                 >  2:950986/1‑138 (MQ=255)
        ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTGATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGGTTTACTGATGATCAGCGCCGTCGTGC‑GTTTGGCCGCCCGCt                                                                                                                              <  7:539471/140‑1 (MQ=255)
         gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTGGccgccccc                                                                                                                               >  2:43022/1‑136 (MQ=255)
         gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGATATATCGCTTTTCTCGGGAAATGACGCTGCGCAAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTGTGGccgccccc                                                                                                                               >  2:547478/1‑136 (MQ=255)
            cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GGTTGGCCGCCcgcgcg                                                                                                                            >  1:676642/1‑138 (MQ=255)
            cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTTGCGGCCCGCTCg                                                                                                                            >  1:235379/1‑138 (MQ=255)
            cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTGGCCGCCCGCTcc                                                                                                                            >  3:117975/1‑137 (MQ=255)
             ggTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑a                                                                                                                          <  2:904549/138‑1 (MQ=255)
             ggTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGGCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑a                                                                                                                          <  2:160360/138‑1 (MQ=255)
              gTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ag                                                                                                                         >  2:785150/1‑137 (MQ=255)
              gTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTTTgcgcccgcgtcat                                                                                                                          >  2:677495/1‑133 (MQ=255)
                  ttATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGtt                                                                                                                   <  7:882873/140‑1 (MQ=255)
                  ttATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGtt                                                                                                                   <  6:25958/140‑1 (MQ=255)
                    aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGtttt                                                                                                                 <  8:639238/140‑1 (MQ=255)
                        cGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGGTGGGCGCCCGCACGCGTTTTGTTTTTTa                                                                                                              >  7:810423/1‑140 (MQ=255)
                         gggATATTTCAATGCCTCGAGCCGTAGAAGGATGAACGCGTTATATCGCTTAGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGCTCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTAcc                                                                                                            <  7:952438/140‑1 (MQ=255)
                          ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCg                                                                                                           <  6:351718/140‑1 (MQ=255)
                          ggAGATTTCAAAGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCg                                                                                                           <  8:704960/140‑1 (MQ=255)
                           gagaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GGTTGGGCGCCCGCGCG‑ATtttttttttttac                                                                                                            >  3:889784/1‑133 (MQ=255)
                           gagaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTGGGCGCCCGCTCG‑ATTTTGTTTTCTACCGt                                                                                                          >  8:171431/1‑140 (MQ=255)
                              aTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTg                                                                                                         <  3:289450/138‑1 (MQ=255)
                                        cTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GTTTGGGCGCCCGCGGC‑CTTTTTTTTTATAAAGTGGTCTGagactcc                                                                                             >  5:16351/1‑134 (MQ=255)
                                        cTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGGCGCCCCCGCG‑GTTTTGTTTTTTTACGTGGGGTGACCGTcc                                                                                             >  5:872582/1‑139 (MQ=255)
                                            cgCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  5:38860/139‑1 (MQ=255)
                                            aGCCGTAGACGGATGAACGCGTTATATCGCTTTGCGAGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGAGGATCAGCGCCGGCGGGC‑GTGTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  6:874189/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGTCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  3:7295/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  3:564371/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  1:764102/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  1:146918/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  4:574557/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  4:725371/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  5:524291/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  7:86743/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  6:826770/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTGGCCGCCCGCTCG‑ATTTTGTTTTATACCGGGGTCTGAACGTCAGCgc                                                                                         >  5:246131/1‑139 (MQ=255)
                                            aGCCGGAGAAGTATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGGTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCGCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCgt                                                                                         <  5:554303/140‑2 (MQ=255)
                                              ccGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  1:130863/138‑1 (MQ=255)
                                              ccGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  3:742014/138‑1 (MQ=255)
                                              aagagacaGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAg                                                                                       <  5:333681/133‑1 (MQ=255)
                                               cGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGc                                                                                      <  5:978394/140‑1 (MQ=255)
                                                 ttgAAGGATGAACGCGTTATATAGCTTTGCGCGTGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                    <  8:101521/138‑1 (MQ=255)
                                                 tAGAAGGTTGAACGCGTTATATCGCTTTGCGCGGGAATTGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGc                                                                                      <  2:726618/138‑1 (MQ=255)
                                                 tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGG‑GTTTGGGCGCCCCCGCG‑CTTTTTTTTTATAACGGGGGTGGAACGTCAGCAAAGCtt                                                                                    >  5:82900/1‑140 (MQ=255)
                                                 tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCGGCCCGCGCG‑ATTTTTTTTTATACCCTTGTCTGAAAGTCAGCGAAGcat                                                                                    >  8:86743/1‑138 (MQ=255)
                                                 tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGc                                                                                      <  4:33168/138‑1 (MQ=255)
                                                    aaGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                 <  6:687388/140‑1 (MQ=255)
                                                     aGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTTGCCGCCCCCGGG‑ATTTTGTTTTTTTACCTTGTGTGAACGTGATCGAAGcgtat                                                                                  >  1:582407/1‑134 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                 <  1:365108/138‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTTGCCGCCCCCTCG‑ATTTTTTTTTATTACGGGGTGTGAAGGTCAGCGAAGCttaggtt                                                                               >  8:434127/1‑135 (MQ=255)
                                                         tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTTTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         >  7:700833/1‑127 (MQ=255)
                                                           aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGGCGCCCGCGCG‑ATTTTTTTTTGTTACGGGGTCTGAACGTCAACGAACCTTCTGTACTGGt                                                                          >  6:218353/1‑140 (MQ=255)
                                                             cgcgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTGGGCGCCCGCGCG‑CTTTTGTTTTGTACCGTGGTGTGAACGTCAGCGCAGCTTATGTACTGGt                                                                          >  2:963621/1‑138 (MQ=255)
                                                               cgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTa                                                                        <  2:687958/138‑1 (MQ=255)
                                                                     atCGCTTTGCGCGGGAAATGTCGCTGAGCCAGTAGCGTTTTACTGATGATAAGCGCCGTCGTGC‑GTTTGGCAGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCt                                                                <  6:550377/140‑1 (MQ=255)
                                                                       cGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGCTGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCt                                                                <  1:990920/138‑1 (MQ=255)
                                                                        gCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCtt                                                               <  2:435894/138‑1 (MQ=255)
                                                                        gCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTcc                                                             <  5:240175/140‑1 (MQ=255)
                                                                           ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTTGGAGCCCGCGCG‑ATTTTTTTTTTTAACGTGGTGTGAACCTCACCGCAGCTTTGGTTCTGGTTTAGCGCGGGTCCg                                                            >  2:842203/1‑138 (MQ=255)
                                                                           ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCg                                                            <  4:472343/138‑1 (MQ=255)
                                                                           ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCGCG‑ATTTTGTTTTCTACCGTGGTCGGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCGTccc                                                            >  4:744324/1‑137 (MQ=255)
                                                                            tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GGTTGGCGGCCCGCTCG‑ATTTTTTTTTATTACGGGGTGGGAACGGGAGCGCATCTTCTGGACGGGGTTAGCGCGCTGcccggc                                                         >  6:333577/1‑136 (MQ=255)
                                                                             gcgcGGGAAATGTCGCTGCGCCAGGTGCGTGTTACTGATGAGGAGCGCCGGCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                                          <  3:418381/138‑1 (MQ=255)
                                                                              cgcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCGCG‑ATTTTGTTTTGTACCGGGGTGTGAACGTCAGCGAAGCGTGTGTTCGGGTTACGCGCGCTTCCgcta                                                         >  4:716446/1‑135 (MQ=255)
                                                                               gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGGCTGACCGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATca                                                        <  1:1011929/138‑1 (MQ=255)
                                                                                cgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GGTTTGGCGCCCGCGCG‑ATTTTTTTTTGTACCGGGGTCTGAACGTCAGCGAAGCGTCAGTACTGGTTAAGCGCGCGTGCGCTcac                                                       >  4:278510/1‑138 (MQ=255)
                                                                                    aaaTGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCg                                                 <  6:860195/140‑1 (MQ=255)
                                                                                        gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                             <  6:622425/140‑1 (MQ=255)
                                                                                        gTCGCTGCGCCAGGTGCGGTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                             <  5:484193/140‑1 (MQ=255)
                                                                                           gCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCTGGC‑GGTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACt                                            <  4:984250/138‑1 (MQ=255)
                                                                                           gCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTGTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACt                                            <  2:569550/138‑1 (MQ=255)
                                                                                                 ccAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGGCGCCCGCGCG‑ATTTTTTTTTATAACGTGGTCTGAACGTCAGCGCAGCGTCTGTTCTGGTTTAGCGCGCTTCCGAACACAATCCCCTACTCGtttgat                                    >  8:450267/1‑136 (MQ=255)
                                                                                                  cAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                   <  8:392151/140‑1 (MQ=255)
                                                                                                  cAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTCGAAtt                                   <  5:385674/140‑1 (MQ=255)
                                                                                                      tGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCa                                 <  1:926597/138‑1 (MQ=255)
                                                                                                      tGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                               <  7:804430/140‑1 (MQ=255)
                                                                                                           tttACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCgg                          <  5:459981/140‑1 (MQ=255)
                                                                                                                  atgatCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                     <  1:334655/138‑1 (MQ=255)
                                                                                                                   tgatCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGcc                    <  3:948091/138‑1 (MQ=255)
                                                                                                                    gatCAGCGCCGTCGGGC‑GTTTGGCCGCCCCCTCG‑GTTTTTTTTTGATACGGGGTAGGAACCTAAGCGAAGCGTATGTACGGGTTTAGGGGGCGTACGCTCAACATAAGCTACTAGTTGTATTAAGTCCCCGCGAGccg                   >  4:65779/1‑137 (MQ=255)
                                                                                                                     atCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTTTTTTATAACGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTTAGCGCGCGTCCGATCACAATACGCTACTCGTTTTATTAAGTACCGGCAAGCCTGaa                >  6:895885/1‑139 (MQ=255)
                                                                                                                        cgcgcCGGCGGGG‑GTTTGGCCGCCCGTTCG‑ATTTTGTTTTCAACCGTTGTCTGATCGTCAGCTAAGCTTCTGTAATGGTTACGCGCGCTTCCGATAACAATCCGCTAATCGTTTAATTCAGTCCCGGCAAGCCTGaaaaa             <  6:368197/139‑1 (MQ=255)
                                                                                                                        aGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGACCGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa             <  6:683840/140‑1 (MQ=255)
                                                                                                                        aGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa             <  7:824855/140‑1 (MQ=255)
                                                                                                                        aGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa             <  5:118422/140‑1 (MQ=255)
                                                                                                                        aGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata               <  4:889784/138‑1 (MQ=255)
                                                                                                                        aGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTATTCTACCGTGGGCTGACCGTCAGCGTAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa             <  8:938640/140‑1 (MQ=255)
                                                                                                                           gcCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa          <  5:510629/140‑1 (MQ=255)
                                                                                                                           gcCGGCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTCCCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa          <  8:174972/140‑1 (MQ=255)
                                                                                                                                   gC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATg    <  1:846070/138‑1 (MQ=255)
                                                                                                                                   gC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc  <  6:124304/140‑1 (MQ=255)
                                                                                                                                   gC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGCTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATg    <  2:137531/138‑1 (MQ=255)
                                                                                                                                    gagTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAAGCGCGCTTCCGATCACAATCCGCTACTAGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATg    <  3:318743/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
ACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCG‑ATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTC  >  NZ_CP022686/3734233‑3734503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A