Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F2 I0 R1 130 6907.4 7549744 98.4% 7428948 136.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,367 T→G 20.7% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3670TG20.7% 220.9 / 19.6 153F225V (TTT→GTT) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (21/97);  new base G (31/4);  total (52/101)
Fisher's exact test for biased strand distribution p-value = 1.94e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

GCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAG  >  NZ_CP022686/3734229‑3734505
                                                                                                                                           |                                                                                                                                          
 cTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGgtt                                                                                                                                          >  1:982204/1‑140 (MQ=255)
   acccacATCGGCACGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATTTCGCTGCGCCAGGTGCGTTTTAATGATGATCAGCGCCGTCGGGCCGTTTg                                                                                                                                         >  4:59298/3‑140 (MQ=255)
    accacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGTTTggg                                                                                                                                       >  4:750963/1‑139 (MQ=255)
           cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTTGcggcacgc                                                                                                                                >  1:429961/1‑140 (MQ=255)
           cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGAGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGTTTTGccgccccc                                                                                                                                >  1:783523/1‑138 (MQ=255)
            ggCCCGGGCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGGCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCt                                                                                                                               <  7:174766/140‑1 (MQ=255)
                cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTGTGGGCGCCCGCGCGag                                                                                                                           >  3:437909/1‑139 (MQ=255)
                cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTGGCCGCCCGCGCGAt                                                                                                                           >  4:396394/1‑140 (MQ=255)
                 ggTCAATATGGAGGGAGAATTCAATCCCTGGAGCCGTAGAAGGATGAACTCGTTATATAGCTTTGAGCGTGAAATGTCGCTGCGCCAGGTTCGTTTTACTGATGATAAGCGCCGTCGGC‑AGTTTGGCCGCCCGATCGAtt                                                                                                                          <  6:287818/140‑1 (MQ=255)
                      ttATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGtt                                                                                                                     <  1:563937/140‑1 (MQ=255)
                      ttATGGCGGGAGATTTCAATGACTGGAGCCGTAGAAGGATGTACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCGAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGtt                                                                                                                     <  7:416690/140‑1 (MQ=255)
                        aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGtttt                                                                                                                   <  2:442294/140‑1 (MQ=255)
                            cGGGAGATTTCAATGCCTGGAGCCGTAGAAGTATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTTGCCGCCCCCTCGATTTTGtttttttg                                                                                                               >  1:704540/1‑136 (MQ=255)
                             gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGTTTTGCCGGCCCCGCGCTttttttttattcc                                                                                                              >  8:479670/1‑135 (MQ=255)
                             gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTGGCCGCCCGCTCGATTTTGTTTtataac                                                                                                              >  6:279808/1‑138 (MQ=255)
                              ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGACAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCg                                                                                                             <  6:440632/140‑1 (MQ=255)
                              ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGGCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGGCGGG‑CGGTGGGCCGCCCGCGCGATTTTGTTTGCTACCg                                                                                                             <  8:389941/140‑1 (MQ=255)
                               gagaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTTGGCGCCCGCGCGATTTTTTTTTGTAGCGt                                                                                                            >  6:727477/1‑140 (MQ=255)
                               gagaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTGGGCGGCCCCGCGgttttgttttgttacgg                                                                                                            >  3:760382/1‑133 (MQ=255)
                                    ttCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGTTTTGCCGCCCCCGCGATTTTGTTTTGTAACGTGGTCt                                                                                                       >  2:908072/1‑140 (MQ=255)
                                     tCAATGCGTGGAGCCGTAGAAGGATGAACTCGTTATATCGCGTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTg                                                                                                      <  7:57586/140‑1 (MQ=255)
                                        aTGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAAc                                                                                                   <  2:568903/140‑1 (MQ=255)
                                           ccTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTGGCCGCCCGCGCGCTTTTGTTTTATACCGTGGGCTGGACGTc                                                                                                >  4:936506/1‑140 (MQ=255)
                                            cTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCCGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGG‑CGGTTGGCCGCCCGGACGATTTTTTTTTATACAGTGGTCTTaaactca                                                                                               >  4:28149/1‑135 (MQ=255)
                                                agacGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCTCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTAACGTGGTCTGAACGTCAGCGa                                                                                           <  2:830428/137‑1 (MQ=255)
                                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGATATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  6:772045/140‑1 (MQ=255)
                                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  2:832039/140‑1 (MQ=255)
                                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  8:186602/140‑1 (MQ=255)
                                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  2:951851/140‑1 (MQ=255)
                                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  2:972221/140‑1 (MQ=255)
                                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  4:158723/140‑1 (MQ=255)
                                                aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  5:33789/140‑1 (MQ=255)
                                                aGCCGTAGAAGGATGAAAGCGTTATAGCGCTTTTCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  4:176696/140‑1 (MQ=255)
                                                     tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGGTTGGGCGCCCCCGCGCTTTTTTTTTTTTACGGGGGGTGAACCTGAGCGCAGCtt                                                                                      >  3:852498/1‑140 (MQ=255)
                                                     tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTTGGCGCCCGCGCGATTTTTTTTTATACCGTTGTGTGAACGTCAGCGAAGcgt                                                                                      >  6:752335/1‑138 (MQ=255)
                                                     tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACAGTTGTCTGAACGGCAGCGAAGCtt                                                                                      <  1:385361/140‑1 (MQ=255)
                                                        aaGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                   <  6:403243/140‑1 (MQ=255)
                                                         aGGATGAACGCGTTATCTCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGTG‑CGTTTGGCCGCCAGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGt                                                                                  <  4:190280/140‑1 (MQ=255)
                                                          ggATGACCGCGTTATATCTCTGTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCGCGATTTTGTTTTCTCCCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  8:368101/140‑1 (MQ=255)
                                                          ggATGAACTCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  6:447539/140‑1 (MQ=255)
                                                          ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  7:761984/140‑1 (MQ=255)
                                                          ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  3:599778/140‑1 (MQ=255)
                                                          ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGACAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  4:696838/140‑1 (MQ=255)
                                                          ggATGAACGCGGTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  5:245194/140‑1 (MQ=255)
                                                            aTGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTTGCCGCCCCCGGGCTTTTTTTTTATAACCTGGTCGTAACGTCAGCGAAGCTTCTGTaat                                                                               >  5:83515/1‑138 (MQ=255)
                                                            aTGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTGTGTACt                                                                               >  6:550358/1‑140 (MQ=255)
                                                             tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGTTTGGGCGCCCGCTCGTTTTTGTTTTATGACGGGGGCGGCACCTGAGCGCAGCGTCTGttgtg                                                                              >  6:484037/1‑136 (MQ=255)
                                                             tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGGGTGGCCGCCCCCACGCTTTTTTTTTATACCGGGGTGTGTACGTGACCGAAACATCTGTaatt                                                                              >  2:311505/1‑137 (MQ=255)
                                                               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCTCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTGTCCGCCCGCTAGATTTTTGTTTATAACTTGGTGTGACAGTAAGAGAAGCGTCTgatcgtgt                                                                            >  4:553536/1‑133 (MQ=255)
                                                               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGTGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTTCCGTGGTCTGAAAGTCAGCGAAGCTTATGTTCTGGt                                                                            >  8:720813/1‑140 (MQ=255)
                                                               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGG‑GGGTTGGGCGCCCGCGCCATTTTTTTTTGTTCAGGTGTGGGAAAGGCAGCCAAGCGTCTGTTATGGt                                                                            >  3:147744/1‑140 (MQ=255)
                                                               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGTTTTGCCGCCCGCGCGAGTTTTTTTTTTTCCGTGGTCGGAACGTCAGCGCAGCGTCTggtggggg                                                                            >  6:532307/1‑133 (MQ=255)
                                                               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGGTTTGCCCCCCGCGCGATTTTGTTTTTTAACGGGGTGTGGACGGCAGCGAAGCGGATGTTCTGGt                                                                            >  6:788695/1‑140 (MQ=255)
                                                               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTGGGCGCCCCCGCGCTTTTGTTTTGTTCCGGGGGGGGAACGTCAGCGCAGCGTGTGTACTggg                                                                            >  6:18946/1‑139 (MQ=255)
                                                               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTGGGCGCCCCCCCGATTTTGTTTTTTAACGGGGGGGGAACGTCAGCGACGCGTCTggtacggt                                                                            >  5:695042/1‑133 (MQ=255)
                                                               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTGGCCGCCCGCGCGATTTTGTTTTATAACGAGGTCGGAACGTCAACGAAGCGTCTGTACAGGt                                                                            >  4:223447/1‑140 (MQ=255)
                                                               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCCGG‑GGGTTTGCCGCCCGCGCGATTTTGTTTTCGACCCTGGTCTGAACGTCAGCGAAGCGTCGTTACTggg                                                                            >  3:837832/1‑139 (MQ=255)
                                                                     ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcg                                                                      <  2:394709/140‑1 (MQ=255)
                                                                     ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTTGGCGCCCGCGCGCTTTTGTTTTGTACCGTGGTCTGAACGTCAGCGCAGCGTCTGTACTGGTTTcgcg                                                                      >  4:6479/1‑140 (MQ=255)
                                                                     ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGGTTGGCCGCCCGCGCGAGTTTTTTTTATTCCGTGGTCTGTACGGGCGCGGCGCTTCTGGTCGGGTTTcgcg                                                                      >  1:388336/1‑140 (MQ=255)
                                                                     ttAGATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGGGCGTTTTACTGATGATCAGCGGCGTCGGG‑CGTTTGGCCGCCCGCGCGATTTTGTTTTCTACGGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcg                                                                      <  1:459317/140‑1 (MQ=255)
                                                                       atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                    <  7:501029/140‑1 (MQ=255)
                                                                       atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                    <  7:389612/140‑1 (MQ=255)
                                                                           cGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                                <  6:548960/140‑1 (MQ=255)
                                                                            gCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTcc                                                               <  4:952025/140‑1 (MQ=255)
                                                                             cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCGCGATTTTTTTTTTTACCGTGGTATGAACGTCAGCGAAGCTTCTGTTCAGGTTAAGCGCGCTTccc                                                              >  4:554940/1‑139 (MQ=255)
                                                                              tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGCGG‑CGGTGGGGCGGCCGCGCCATTTTGTTTTTGAGCGGGGGGTGAAAGTCAGGGACGGGTGTGTAAGGGTTTCGGGCGCtggcgc                                                             >  3:207811/1‑135 (MQ=255)
                                                                              tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGGTTGGCCGCCCGCGGGCTTTTGTTTTGTTCCGTGGGGGGCACGTCCGCGGAGCGTGGGTAATGGGTACgcgcgcgtgcgc                                                             >  4:464448/1‑134 (MQ=255)
                                                                                 gcgcGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATca                                                          <  2:179169/140‑1 (MQ=255)
                                                                                   gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCTCCCGCTCGATTTTGATTTCTACAGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATaaca                                                        <  5:458959/140‑1 (MQ=255)
                                                                                   gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                                        <  5:279808/140‑1 (MQ=255)
                                                                                     gggCAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGACGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAAt                                                      <  1:320541/140‑1 (MQ=255)
                                                                                       gataTGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATcc                                                    <  7:281474/137‑1 (MQ=255)
                                                                                            gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                               <  4:469089/140‑1 (MQ=255)
                                                                                                      cAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑GGGTTGGGCGCCCCCGCGCTTTTTTTTTCTACCGTGGTGTGAACCTAAGCCACGCGTATGTTAAGGGTTCGCGCGCGTCCGATCCCCATACGCGACTAGTTGGAtt                                     >  4:800658/1‑140 (MQ=255)
                                                                                                      cAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCTCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                     <  2:585689/140‑1 (MQ=255)
                                                                                                      cAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                     <  5:418924/140‑1 (MQ=255)
                                                                                                      cAGGTGCGTTTTACTGATGATCAGCGAAGTCTGG‑CGTTTTGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCAAAATCCGCTACTCGTTGAAtt                                     <  7:629932/140‑1 (MQ=255)
                                                                                                                ttACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                           <  2:189929/140‑1 (MQ=255)
                                                                                                                    tgatgaTCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                       <  6:103768/140‑1 (MQ=255)
                                                                                                                      atgatCAGCGCCGTCGGG‑GGTTTTGCCGCCCGCTCGATTTTTTTTTATACCGTGGTCTGAACGTCAGCCCAGCTTCTGTACTGGTTAAGCGCGCGTCCGCTAACAAAACACAACTAGTTTAAATAAATCCCGGCCAGccg                     >  2:920096/1‑139 (MQ=255)
                                                                                                                      atgatCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCTAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCTCTTCTCGTTGAATTCAGTCCCGGCAAGCCt                     <  1:854242/140‑1 (MQ=255)
                                                                                                                      atgatCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                     <  2:5387/140‑1 (MQ=255)
                                                                                                                      atgatCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                     <  8:715105/140‑1 (MQ=255)
                                                                                                                      atgatCAGCGCCGGCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                     <  6:712305/140‑1 (MQ=255)
                                                                                                                       tgatCAGCTCCGTCGGG‑CGTTTGGCCGCCCGCTCGCTTTTGTTTTCTTCCGTGGTCTGAACGTCATCGAAGCTTCTGTACTGGTTACGCGCGCGTACGAGCACCATCCGCTACTCGTTGAATTCAGTCACGGCAAGCCTg                    <  6:129583/140‑1 (MQ=255)
                                                                                                                        gatCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCGCGAGTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGa                   <  5:380694/140‑1 (MQ=255)
                                                                                                                            cgcgcCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  1:581823/139‑1 (MQ=255)
                                                                                                                            aGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  2:128404/140‑1 (MQ=255)
                                                                                                                            aGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTGTTCTACCGTGGTCTGAACGTCAGCGAAGCTGCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  6:208168/140‑1 (MQ=255)
                                                                                                                               gcctgcGGG‑CGTTTGGCCGCCCGCTAGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCTCTTCCGATCACAATGCGCTACTCGTTGAATTCAGTCCCTGCAAGCCTGataaataa            <  8:25982/135‑1 (MQ=255)
                                                                                                                               gcCGTCGTG‑CGTCTGGCCGCCCGCTCGAGTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAATCAGTCCCGGCAAGCCTGataaataa            <  8:268443/140‑1 (MQ=255)
                                                                                                                               gcCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  3:113102/140‑1 (MQ=255)
                                                                                                                               gcCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  5:903126/140‑1 (MQ=255)
                                                                                                                               gcCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  1:303944/140‑1 (MQ=255)
                                                                                                                                      gg‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGt     <  8:807102/140‑1 (MQ=255)
                                                                                                                                       g‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc    <  3:232244/140‑1 (MQ=255)
                                                                                                                                       g‑CGTTTGGCCGCCCGCTCGATTTTGTGTTCTACCGTGGTCTGCACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATggc    <  8:114754/140‑3 (MQ=255)
                                                                                                                                          gTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAg  <  4:110289/140‑1 (MQ=255)
                                                                                                                                          gTTTGGCCGCCCGCTAGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAATCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAg  <  2:426618/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGG‑CGTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAG  >  NZ_CP022686/3734229‑3734505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A