Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F2 I0 R1	145	5829.6	7950970	98.4%	7823754	135.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	3,734,367	T→G	24.5%	F225V (TTT→GTT)	yafD →	endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	3,734,367	0	T	G	24.5%	204.5 / 28.9	163	F225V (TTT→GTT)	yafD	endonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand): ref base T (27/93); new base G (38/5); total (65/98)
Fisher's exact test for biased strand distribution p-value = 2.06e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

CTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAG  >  NZ_CP022686/3734230‑3734505

  caccacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTgg                                                                                                                                         >  4:482951/1‑140 (MQ=255)

   accacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGCGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTTgg                                                                                                                                         >  7:379543/1‑140 (MQ=255)

   accacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGCGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGgc                                                                                                                                        >  7:75300/1‑140 (MQ=255)

   accacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTggg                                                                                                                                        >  5:238890/1‑139 (MQ=255)

     cacaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTggg                                                                                                                                        >  1:725942/1‑137 (MQ=255)

     cacaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGggcg                                                                                                                                      >  5:307563/1‑140 (MQ=255)

      acaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGG‑GTTTGgcgc                                                                                                                                      >  8:914856/1‑138 (MQ=255)

       caGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GTTTGgccg                                                                                                                                      >  1:988825/1‑138 (MQ=255)

          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCTCTGCGACAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGC‑GGTTGGccgcccgc                                                                                                                                 >  5:726559/1‑140 (MQ=255)

          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGccgccccc                                                                                                                                 >  4:174704/1‑138 (MQ=255)

          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGC‑GGTTTGacgcgcgc                                                                                                                                 >  7:528923/1‑140 (MQ=255)

          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGGGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGGTTGGccccccc                                                                                                                                  >  7:528924/1‑139 (MQ=255)

          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCGGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGAGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTG‑GGGG‑GGTTGGgcgcccgc                                                                                                                                 >  8:193582/1‑140 (MQ=255)

           ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑AGGC‑GTTTGGCCGCCCGCt                                                                                                                                <  3:685088/140‑1 (MQ=255)

            gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTTGccgcccgc                                                                                                                                 >  2:807622/1‑138 (MQ=255)

             cccGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGG‑GTTTGGccccccctcg                                                                                                                               >  5:401207/1‑136 (MQ=255)

             cccGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGgcgcccgcgag                                                                                                                              >  6:624512/1‑137 (MQ=255)

               cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTG‑GCGC‑GGTTGGgcgcccccacggg                                                                                                                            >  7:806397/1‑133 (MQ=255)

               cGGGCATTAGGGCGGGAGATTTCAATGGCTGGACCCGTAGAAGGATTAACGCGTAATATAGCTATGAGCGTGAATTGTCGCTGCGCGAGGTGCGTTTTACTGATGTACAGCGCCGTC‑GGGC‑TGTTTGCAGcccgcccgtg                                                                                                                            >  4:866779/1‑138 (MQ=255)

                gggcATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGa                                                                                                                             <  2:526931/135‑1 (MQ=255)

                ggTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGAtt                                                                                                                           <  4:298220/140‑1 (MQ=255)

                 gTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGGGCGCCCCCTCGAt                                                                                                                            >  2:91828/1‑138 (MQ=255)

                     ttATGGCGGGAGATTTCAATGCCTGGAGCCGTATAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCTCTGAGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGtt                                                                                                                      <  3:933242/140‑1 (MQ=255)

                     ttATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGATATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGtt                                                                                                                      <  4:773265/140‑1 (MQ=255)

                      tATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTTGACGCCCGCGCGAtttttttt                                                                                                                     >  5:872095/1‑140 (MQ=255)

                            gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                               <  6:19110/140‑1 (MQ=255)

                            gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                               <  5:25945/140‑1 (MQ=255)

                            gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGGGCGCCCGCACGCGTTTTTTTtata                                                                                                                 >  2:728608/1‑138 (MQ=255)

                            gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑CTTTTGGCGCCCGCGCGATTTTGTTTTATAcc                                                                                                               >  8:346592/1‑140 (MQ=255)

                             ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCg                                                                                                              <  3:161485/140‑1 (MQ=255)

                             ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGGCCGCCCGCGCGATTTTTTTTTGTaa                                                                                                                >  1:154861/1‑137 (MQ=255)

                             ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGGC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCg                                                                                                              <  8:392404/140‑1 (MQ=255)

                              gagaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCATTGCGCGGGAAATGTCGATGCGCCAGGTTCGTTTTACTGATGATCAGCGCCGTC‑CGGC‑GTGTTGGCACCCCCGCGTTTTTTTTATCTaacat                                                                                                             >  6:947051/1‑136 (MQ=255)

                                          ccTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGA‑GTTTTGCCGCCCGCTCGAGTTTGTTTTATAACGAGGGCTGAACCTc                                                                                                 >  5:543229/1‑140 (MQ=255)

                                            tGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GTTTTGGCGCCCCCGCGATTTTTTTTTTTAAACTGTTCTGAACGta                                                                                                 >  1:207122/1‑137 (MQ=255)

                                            tGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGGGCGCCCGCGCGATTTTTTTTTATAACGTGGTCTGAAAGTCac                                                                                               >  3:512294/1‑139 (MQ=255)

                                               tgCCGTAGAAGGATGACCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCTATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                            <  8:216505/139‑1 (MQ=255)

                                               tgCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCGCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                            <  4:170062/139‑1 (MQ=255)

                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                              <  2:725747/138‑1 (MQ=255)

                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                            <  4:285007/140‑1 (MQ=255)

                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                            <  8:467801/140‑1 (MQ=255)

                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                            <  4:415243/140‑1 (MQ=255)

                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                            <  7:823161/140‑1 (MQ=255)

                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                            <  3:779038/140‑1 (MQ=255)

                                               aGCCGTAGAAGGATGAACGCGTGATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                              <  2:51278/138‑1 (MQ=255)

                                               aGCCGTAGAAGGAGGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                              <  1:3128/138‑1 (MQ=255)

                                                gCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGaa                                                                                           <  5:200771/140‑1 (MQ=255)

                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTG‑GGGG‑GGTTTGGCGCCCGCTCGCGTTTGTTTTATTCCGGGGTCTGACAGTGAGCGCAgggt                                                                                       >  3:552178/1‑137 (MQ=255)

                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTG‑GGGG‑GGTTGGGCGCCCGGGCGCTTTTTTTTTTTAACGTGGTCTGAAAGTAAGCGAAgggt                                                                                       >  4:991347/1‑137 (MQ=255)

                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                       >  7:221744/1‑140 (MQ=255)

                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                       <  4:452918/140‑1 (MQ=255)

                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGGGCGCCCCCTCGCTTTTTTTTTATTACGTGGTCTGGACGTCAGCGAGGcgt                                                                                       >  8:249905/1‑138 (MQ=255)

                                                         ggATGAACGCGTTATATCTCTTTGCGCGGGAAATGGCGCTGCGCCAGGTGCGTTTTACTGCTGATCAGCGCCGGC‑GGGC‑GGGTGGCCGCCCGCTCGATTTTGATTTCTCCCGTGGGCTGAACGTCAGCGAAGCTTCT‑g                                                                                   <  1:944017/138‑1 (MQ=255)

                                                         ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTa                                                                                 <  3:302358/140‑1 (MQ=255)

                                                         ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTa                                                                                 <  6:765537/140‑1 (MQ=255)

                                                         ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTa                                                                                 <  4:502823/140‑1 (MQ=255)

                                                           aTGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTGGGCGCCCCCGCGCTTTTTTTTTTTAACCGGGGGTGGACGGCAGAGCAGCTTcgggaag                                                                                >  3:847824/1‑134 (MQ=255)

                                                            tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTTGCCGACACCTCGAATTTTTTTTCTTGCGTGGTGAGAAGCTCAGCTCAGGATCT‑GTTCTg                                                                              >  4:90331/1‑140 (MQ=255)

                                                              aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GTTTGGCCGCCCCCGCGATTTTTTTTTTTACCGTTGTGTGAAAGTAAGCGCAGCATAT‑GTTATGGt                                                                            >  8:34155/1‑140 (MQ=255)

                                                              aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGGTGTCCCCCCCGCCCCGTTTGTTTTGTGACGAGGTGGGGACGTCAGCGAAGCGTGT‑GGTCTGGt                                                                            >  3:2462/1‑140 (MQ=255)

                                                              aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTTGGCGCCCGCGCGCGTTTGTTTTATTCCGTGGTCTTAACGTCAGCGAAGCTTCT‑GTaaaggg                                                                            >  7:603647/1‑135 (MQ=255)

                                                                cgcgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTTGCCCCCCCCACCCATTTTG‑TTTGTACACGGGTGTGAAACTCACCGAAGCGTGT‑GTAATGGt                                                                            >  1:968502/1‑138 (MQ=255)

                                                                     tataTCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTGGCCGCCCGCACGCTTTTTTTTTGTTACGTGGTGTGAACGTAATAGAACCTTCA‑GTACTTGtttagc                                                                       >  2:339955/1‑134 (MQ=255)

                                                                      atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTAcgcgcg                                                                    <  6:163215/140‑1 (MQ=255)

                                                                      atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTAcgcgcg                                                                    <  6:516242/140‑1 (MQ=255)

                                                                           gCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTA‑GGGC‑GGTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGACCGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTcc                                                               <  7:550175/140‑1 (MQ=255)

                                                                             tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTcc                                                               <  1:634630/138‑1 (MQ=255)

                                                                              ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGAt                                                            <  4:780530/140‑1 (MQ=255)

                                                                               tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTGGACCCCCCCTCGATTTTGTTTTCTAACGTGGTATGCACGTCAGCGAAGCTTGT‑GTTCTGGTTAAGCGCGCGTCCgcta                                                           >  6:542739/1‑137 (MQ=255)

                                                                                  gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATca                                                          <  1:277445/138‑1 (MQ=255)

                                                                                    gggAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAAt                                                      <  4:860952/140‑1 (MQ=255)

                                                                                              gCTGCGCCAGCTGCGTTTTACTGATGATCAGCTCAGTC‑GGGC‑GTTTGGCCGCCCGCTCTATTTTGTTTTCTACCGTTGTCTGAACGTCAGCGAAGCTTCT‑GTTCTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCg                                            <  6:116003/140‑1 (MQ=255)

                                                                                                tGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGtt                                          <  7:131026/140‑1 (MQ=255)

                                                                                                tGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGtt                                          <  3:174704/140‑1 (MQ=255)

                                                                                                    ccAGGTGCGGTTTACTGTTGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAt                                      <  5:145746/140‑1 (MQ=255)

                                                                                                     cAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                     <  3:406907/140‑1 (MQ=255)

                                                                                                     cAGGTGCGGTTTACTGATGATCAGCGCCTTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                     <  5:764988/140‑1 (MQ=255)

                                                                                                            gTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGGCCGCCCCCGCCAATTGTTTTTCTAACGTTGGCAGAAAGGCAGCGCAGCTTGG‑GTGCGGGTTACCCGCGCGTCCCCTCACCAACCGCGACGAGTTGAAATTAGTcac                              >  4:352361/1‑138 (MQ=255)

                                                                                                             ttttACTGATGATCAGCGCCGTC‑GGGG‑GGTTGGCCGCCCGCTCGCTTTTGTTTTGTAACGTGGTCTGAAAGTCAGCGCAGCTTAG‑GTAAGGGTTTCGCCCGCTTCCGCTCACAATACGCTACTCGTTGAATTCAGTcccc                             >  4:589798/1‑139 (MQ=255)

                                                                                                               ttACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCg                             <  1:846735/138‑1 (MQ=255)

                                                                                                               ttACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                           <  6:634037/140‑1 (MQ=255)

                                                                                                                   tgatgaTCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                       <  7:49852/140‑1 (MQ=255)

                                                                                                                     atgatCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                     <  5:902432/140‑1 (MQ=255)

                                                                                                                     atgatCAGCGCCGGC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGCACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                     <  3:333616/140‑1 (MQ=255)

                                                                                                                      tgatCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                    <  4:409292/140‑1 (MQ=255)

                                                                                                                         tCAGCGCCGTC‑GGGC‑GTTTGGGCGCCCGCTCGAATTTGTTTTGTTACGTGGGGGGAACCTCAGCGACGCGTCTGGTCGGGGTTAC‑CGCGCCTCCGCTACCAATGCCCGTCGAGTTGTAGTCAGTCCCCGCAAGCCAGa                   >  2:701696/1‑138 (MQ=255)

                                                                                                                          cAGAGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataa                <  6:974204/140‑1 (MQ=255)

                                                                                                                           aGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  7:396152/140‑1 (MQ=255)

                                                                                                                           aGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata                 <  2:65473/138‑1 (MQ=255)

                                                                                                                              gcCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  4:1009976/140‑1 (MQ=255)

                                                                                                                              gcCGTC‑GGGC‑GTGTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGGCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  6:587454/140‑1 (MQ=255)

                                                                                                                              gcCGTC‑GGGC‑GGTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaat              <  2:919637/138‑1 (MQ=255)

                                                                                                                               ccGTC‑GGGC‑GTTGGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAg           <  8:536109/140‑1 (MQ=255)

                                                                                                                                cggc‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGCACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg          <  3:407903/137‑1 (MQ=255)

                                                                                                                                cggc‑GGGC‑GGTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg          <  6:809832/137‑1 (MQ=255)

                                                                                                                                cGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  2:708868/138‑1 (MQ=255)

                                                                                                                                cGTC‑GGGC‑GGTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg          <  6:403902/140‑1 (MQ=255)

                                                                                                                                       gC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATg      <  2:998386/138‑1 (MQ=255)

                                                                                                                                       gC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc    <  7:672950/140‑1 (MQ=255)

                                                                                                                                       gC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc    <  4:982317/140‑1 (MQ=255)

CTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCT‑GTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAG  >  NZ_CP022686/3734230‑3734505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A