Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F36 I0 R1 271 6613.7 10404966 98.5% 10248891 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,367 T→G 18.7% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3670TG18.7% 301.1 / 28.1 194F225V (TTT→GTT) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (39/115);  new base G (36/4);  total (75/119)
Fisher's exact test for biased strand distribution p-value = 5.11e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

CTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAG  >  NZ_CP022686/3734230‑3734505
                                                                                                                                          |                                                                                                                                          
cTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑Gttt                                                                                                                                          >  6:438985/1‑140 (MQ=255)
cTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑Gttt                                                                                                                                          >  6:953572/1‑140 (MQ=255)
   accacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGCGGTTTgg                                                                                                                                        >  5:1184827/1‑140 (MQ=255)
   accacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGgc                                                                                                                                       >  2:204165/1‑140 (MQ=255)
   accacAGCGGCACGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAAAGCGTTATATCGCTTTGCGCGTGAAATGTCGCTGCGCCAGGTGCGTTTTACTTATGATCATCGACGTCGGGC‑GGTTGgc                                                                                                                                       >  8:609877/1‑140 (MQ=255)
      acaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGA‑GGTTGgccgc                                                                                                                                    >  6:1223188/1‑140 (MQ=255)
        aGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTTGccgccc                                                                                                                                  >  4:639854/1‑140 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCCGCGCCGTCGGGC‑GGGTGGgcccccgc                                                                                                                                >  5:780974/1‑140 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGC‑GTGTTGcagcccgc                                                                                                                                >  7:302679/1‑140 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGC‑GGTTGGgcggccgc                                                                                                                                >  2:1348057/1‑140 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GTTTGGccgcccgc                                                                                                                                >  8:1111894/1‑140 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGTccgcccgc                                                                                                                                >  6:554565/1‑140 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGccgcccgc                                                                                                                                >  2:620811/1‑140 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGccgcccgc                                                                                                                                >  6:641559/1‑140 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTGGccgcccgc                                                                                                                                >  4:356477/1‑140 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCCGGC‑GGTTGGccgcccgc                                                                                                                                >  3:1194772/1‑140 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGATTGCACGGCCTGGAGCCGTAGAAGGAGGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTAGTACGGAGGATCAGAGCCGTCGGGG‑GTTTTgccggccgg                                                                                                                                >  8:177019/1‑139 (MQ=255)
          cGGCCCGGTCATTATGGCGGGAGAAATCAATGCCTGGAGCCGTAGAAGGAGGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGAGCCGTCCGGG‑GTTTGGccccgcgc                                                                                                                                >  2:380950/1‑140 (MQ=255)
             cccGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGTCCGCCcgcgcg                                                                                                                             >  1:638483/1‑140 (MQ=255)
               cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTGGGGC‑GGGTGGccgcccccacgcg                                                                                                                           >  3:877631/1‑133 (MQ=255)
               cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTGGCCCCCCGCACGAt                                                                                                                           >  1:1292367/1‑140 (MQ=255)
               cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCCGGG‑GTTTGTCCGCCCGCACggt                                                                                                                           >  4:32931/1‑138 (MQ=255)
                gggcATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGAtt                                                                                                                          <  1:1018923/137‑1 (MQ=255)
                ggTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGAtt                                                                                                                          <  5:438985/140‑1 (MQ=255)
                ggTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGAtt                                                                                                                          <  1:204165/140‑1 (MQ=255)
                   cATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCACCCGCTCGATTTTg                                                                                                                       <  6:425016/140‑1 (MQ=255)
                     ttATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGtt                                                                                                                     <  3:1116996/140‑1 (MQ=255)
                       aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GGTTGTGCGCCCCGCCGAGtttttttt                                                                                                                   >  5:202512/1‑140 (MQ=255)
                       aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGtttt                                                                                                                   <  2:1107301/140‑1 (MQ=255)
                            gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCTGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                              <  7:423228/140‑1 (MQ=255)
                            gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                              <  1:1125437/140‑1 (MQ=255)
                            gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                              <  2:952253/140‑1 (MQ=255)
                            gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTGTTGCCGCCCCCGCGATTTTTTTTTGTAcc                                                                                                              >  3:695065/1‑140 (MQ=255)
                             ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCg                                                                                                             <  4:1265911/140‑1 (MQ=255)
                             ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCg                                                                                                             <  3:375695/140‑1 (MQ=255)
                              gagaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCGGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GGTTGTGCCCCCGCTGGATtttttttttgtacgg                                                                                                            >  7:405923/1‑133 (MQ=255)
                              gagaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GTTTGGCCGCCCGCGCGATTTTGTTTtatatgct                                                                                                            >  1:732985/1‑136 (MQ=255)
                                 aTTGCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGt                                                                                                         <  5:1079976/140‑1 (MQ=255)
                                    tCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGCTGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTg                                                                                                      <  7:207689/140‑1 (MQ=255)
                                               atccGTAGAAGGATGATCGAGTTATATCGCTTTGCGCTGGAAATGTCGCTGCGCCAGGTGCGATTTACTCATTATCAGCGCCGGCGTGC‑GTTTGGCCGCACGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  3:488645/138‑1 (MQ=255)
                                               aGCCGTAGAAGGCTGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  2:559361/140‑1 (MQ=255)
                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  3:28899/140‑1 (MQ=255)
                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  6:718127/140‑1 (MQ=255)
                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  5:489456/140‑1 (MQ=255)
                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  1:767187/140‑1 (MQ=255)
                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  4:499588/140‑1 (MQ=255)
                                               aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  3:714436/140‑1 (MQ=255)
                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGGCGGCCGCGCGGTTTTTTTTTGTACCGTGGTGTGGACGTCAGCggcgggt                                                                                      >  6:334839/1‑134 (MQ=255)
                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                      <  1:567690/140‑1 (MQ=255)
                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                      <  6:352520/140‑1 (MQ=255)
                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                      <  1:269576/140‑1 (MQ=255)
                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                      <  5:600129/140‑1 (MQ=255)
                                                    tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTTGGCGCCCGCGCGATTTTGTTTTGTAACGTGGTGTGAACGTCAGCGAAGcgt                                                                                      >  6:1038214/1‑138 (MQ=255)
                                                         ggATGACCGCGTTATATCGCTTTGCGCTGGAAATGTCGCTGCGCCAGGTTCGTTTTACTGTTGATCAGCGCCGTCGGTC‑GTTTGGCCGCCCGCTCGATGTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  4:755515/140‑1 (MQ=255)
                                                         ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  7:179841/140‑1 (MQ=255)
                                                         ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  5:127677/140‑1 (MQ=255)
                                                         ggATGAACGCGATATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  2:931627/140‑1 (MQ=255)
                                                            tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGAGC‑GGTTGGCCGCCCCCGCGATTTTGTTTTGTAACGGGGGCTGCTCGTCAGCGCAgctgcttgtctg                                                                              >  4:244283/1‑134 (MQ=255)
                                                              aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GGTTTGCCGCCCGCGCGGTTTTTTTTTGTTCCGTGGTGTGATCGTCAGCGCAGCTTCTgggatggt                                                                            >  3:534108/1‑133 (MQ=255)
                                                                    ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTTGCCCCCCGCTCGCTTTTTTTTTATAACCTGGTGTGAAACTCAGCCAAGATTATGTACTTGTTAcccg                                                                      >  2:973279/1‑140 (MQ=255)
                                                                      atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTTCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                    <  1:888367/140‑1 (MQ=255)
                                                                      atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                    <  7:146654/140‑1 (MQ=255)
                                                                          cGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                                <  7:1153654/140‑1 (MQ=255)
                                                                            cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GGTTGGGCGCCCGCTCGGTGTTTTTTTAAAACGTGGTCTGAACGTAAGCGCAGCATAGGTTATGGTTTAgcgcgcgagcc                                                              >  6:910707/1‑135 (MQ=255)
                                                                            cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGG‑GGTTGGGCGCCCGCGCGAGTTTGTTTTATTACGGGGTCGGGACGTCAGCGCAGCGTGTGTTAGGGTTACGCGCGCGTCCg                                                              >  7:1013835/1‑140 (MQ=255)
                                                                            cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTGGCCGCCCGCGCGATTTTTTTTTGTACCGTGGTGTGAACGTAAGCGAAGCGTGTGTTCTGGGTTAGCGCGCGTCCg                                                              >  8:336599/1‑140 (MQ=255)
                                                                             tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGGCGCCCGCTCCATTTTTTTTTATAAACTGGTATGGACGTAAGCGCAGCTTATGTTAAGGGTACACGCGCAGCCGa                                                             >  2:822087/1‑140 (MQ=255)
                                                                             tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGGCGCCCGCGCGATTTTTTTTTCTACCGGGGTCTGGACGTCAGCGAAGCGTATGTAATGGTTACGCGCGCTTGCGa                                                             >  4:132677/1‑140 (MQ=255)
                                                                              ttGCGCGGGAAATGTCGCTTCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                                            <  8:642621/140‑1 (MQ=255)
                                                                              ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGGCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                                            <  1:1328877/140‑1 (MQ=255)
                                                                               tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGAtttt                                                                                                                        <  4:703127/79‑1 (MQ=255)
                                                                               tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GGTTTGCCGCCCGCGGGATTTTTTTTTGTACCGGGGGCGGAACCTCAGCGACGCTTCGGGTAAGGGTACGCGCGCTTCCGATc                                                           >  1:1268611/1‑140 (MQ=255)
                                                                                  gcgGGAAATGTCGCGGCGCCAGGTGCGTTTTACTGCTGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGGGGTCTGAATGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                                        <  1:394933/140‑1 (MQ=255)
                                                                                       aaaTGTCGCGGCGCCAGGTGCGTTGTACTGAGGCTCAGCGCCGTCGGGC‑GTTTGGCCTCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCTCTTCCGATCACAATCCg                                                   <  1:352864/140‑1 (MQ=255)
                                                                                           gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                               <  1:1028865/140‑1 (MQ=255)
                                                                                           gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                               <  2:186659/140‑1 (MQ=255)
                                                                                           gTCGCTGCGCCAGGTGCGGTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                               <  6:257367/140‑1 (MQ=255)
                                                                                                tGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGtt                                          <  5:910707/140‑1 (MQ=255)
                                                                                                     cAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                     <  4:1065352/140‑1 (MQ=255)
                                                                                                         tGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                 <  2:850589/140‑1 (MQ=255)
                                                                                                               ttACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                           <  8:447899/140‑1 (MQ=255)
                                                                                                               ttACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                           <  1:499000/140‑1 (MQ=255)
                                                                                                                   tgatgaTCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGTTAGATTCAGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                       <  6:192061/140‑1 (MQ=255)
                                                                                                                     atgatCAGCGCCGTCGGGC‑GGTTGGGCGCCCGCGCGGTTTTTTTTTGTACCGTGGTGTGAACGTCAGCCCAGCGTGTGGTCTGGTTTAGCGCGCGTGCGATGACAATCCGCGACTCGTTGAATTCATTCCCCGCAAGccg                     >  2:1314615/1‑139 (MQ=255)
                                                                                                                      tgatCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                    <  6:15218/140‑1 (MQ=255)
                                                                                                                      tgatCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                    <  3:132677/140‑1 (MQ=255)
                                                                                                                      tgatCAGCGCCGTCGGGC‑GGTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGACCGTCAGCGAAGCTTCTGTAATGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                    <  5:1016076/140‑1 (MQ=255)
                                                                                                                       gatCAGCGCCGTCTGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGa                   <  5:548203/140‑1 (MQ=255)
                                                                                                                           aGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTTCCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  8:1124236/140‑1 (MQ=255)
                                                                                                                           aGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  4:141770/140‑1 (MQ=255)
                                                                                                                           aGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  5:1089787/140‑1 (MQ=255)
                                                                                                                           aGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  5:695559/140‑1 (MQ=255)
                                                                                                                           aGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGCTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  3:1079317/140‑1 (MQ=255)
                                                                                                                             cgcCGTCGGGC‑GTTTGGCCGCCCGCGCGGTTTTGTTTTGTACCGGGGGCTGAACGTCAGCGAAGCTTATTTACTGGTTACGCGCGCGTCCGAGCACAAAACGCAACGCGTTGAATTAAGTACCCGCAATCCGGataaata             >  8:791660/1‑140 (MQ=255)
                                                                                                                              gcCGTCGGGC‑GTTTTGCCGCCCGCTAGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  6:1050698/140‑1 (MQ=255)
                                                                                                                              gcCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  2:479620/140‑1 (MQ=255)
                                                                                                                              gcCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  1:19852/140‑1 (MQ=255)
                                                                                                                                cGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTTCCGTGGTCTGAACGTCAGCGAAGCTTCTGTTCTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg          <  8:902624/140‑1 (MQ=255)
                                                                                                                                      gC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc    <  1:1142812/140‑1 (MQ=255)
                                                                                                                                      gC‑GGTTGGCCGCCCGCTGGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGTAGCTTCTGTACTGGTTACGCGCGCGTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc    <  7:989006/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGC‑GTTTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAG  >  NZ_CP022686/3734230‑3734505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A