Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F36 I0 R1 151 8012.5 7490824 98.1% 7348498 134.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,367 T→G 21.1% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3670TG21.1% 208.8 / 26.1 147F225V (TTT→GTT) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (18/94);  new base G (32/2);  total (51/96)
Fisher's exact test for biased strand distribution p-value = 4.13e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

ACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTC  >  NZ_CP022686/3734233‑3734503
                                                                                                                                        |                                                                                                                                          
accacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCTCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTggg                                                                                                                                       >  8:801959/1‑139 (MQ=255)
  cacaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GTTTggg                                                                                                                                       >  3:106661/1‑137 (MQ=255)
  cacaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTgtg                                                                                                                                       >  5:181387/1‑136 (MQ=255)
  cacaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGCGGTTTgg                                                                                                                                        >  5:677952/1‑138 (MQ=255)
  cacaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTCCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGC‑GTTTgtg                                                                                                                                       >  1:647799/1‑136 (MQ=255)
   acaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTG‑GGGG‑GGTTgggc                                                                                                                                      >  5:89025/1‑136 (MQ=255)
   acaGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑Gttttggc                                                                                                                                      >  2:284488/1‑134 (MQ=255)
    caGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGACCGCGTAATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGGTGg‑ccg                                                                                                                                    >  6:318668/1‑138 (MQ=255)
    caGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCGGGGG‑GTTTGg‑cg                                                                                                                                     >  6:75037/1‑137 (MQ=255)
    caGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGg‑gcg                                                                                                                                    >  1:121753/1‑138 (MQ=255)
       cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTTG‑gcgccc                                                                                                                                 >  5:580553/1‑138 (MQ=255)
        ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑ccgcccg                                                                                                                                <  5:523396/138‑1 (MQ=255)
        ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑ccgcccg                                                                                                                                <  2:954916/138‑1 (MQ=255)
         gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTG‑GGGC‑GTTTGG‑gggccccc                                                                                                                               >  6:237021/1‑136 (MQ=255)
         gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTTG‑gcgccccc                                                                                                                               >  2:927816/1‑136 (MQ=255)
         gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTGTGG‑gccccccc                                                                                                                               >  5:77968/1‑136 (MQ=255)
         gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGG‑ccgcccgc                                                                                                                               >  6:37144/1‑138 (MQ=255)
         gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑CGTTGT‑ccccccgc                                                                                                                               >  3:933206/1‑138 (MQ=255)
             ggTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGCAGGATGAACGCGTTATATCGCTTAGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTTATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCAGCTCGAt‑t                                                                                                                        <  7:236537/140‑1 (MQ=255)
              gTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGG‑ccgcccgggagcg                                                                                                                          >  3:940296/1‑132 (MQ=255)
              gTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGC‑GGTTGG‑CCGCCCGCTGGAt                                                                                                                          >  2:725369/1‑138 (MQ=255)
                cATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGC‑CGTTTG‑ACGACCCCACGAt‑tt                                                                                                                       >  4:19655/1‑138 (MQ=255)
                   tATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGt                                                                                                                    >  3:533356/1‑138 (MQ=255)
                    aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGtt                                                                                                                   <  4:926943/138‑1 (MQ=255)
                       gCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTc                                                                                                                <  4:444202/138‑1 (MQ=255)
                        cGGGAGATATCAATGCCTGGAGCCGTAGAAGGATGAACGCGTGATATCGCTTTGCGCGGGCAATGTCCCTGCGCCAGGTGCGTTTTACTGATGATCCTCGCCGTC‑GGGC‑GGGTTG‑CCGCCCCGACCAT‑TGTGTTTTTTAc                                                                                                             >  7:344897/1‑140 (MQ=255)
                         gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTa                                                                                                              <  2:322896/138‑1 (MQ=255)
                         gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTAATGATGATCAGCGCCGTC‑GCGG‑GATTGG‑GCCCCCCCGCCAA‑ttttttttatt                                                                                                              >  2:125107/1‑135 (MQ=255)
                          ggAGATTTCACTGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTAc                                                                                                             <  5:395052/138‑1 (MQ=255)
                          ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTTCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTAc                                                                                                             <  4:387731/138‑1 (MQ=255)
                          ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTGG‑GCGCCCCGGGGCT‑TTTGTTTTGTaa                                                                                                             >  3:273634/1‑137 (MQ=255)
                          ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTGG‑CCGCCCCCTCGCT‑TTTTTTTTGTaa                                                                                                             >  4:917920/1‑137 (MQ=255)
                          ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTGATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTAc                                                                                                             <  2:418712/138‑1 (MQ=255)
                            agaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGG‑GCGCCCGCTCGAT‑TTTTTTTTGTAccc                                                                                                           >  5:309786/1‑137 (MQ=255)
                              aTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTg                                                                                                         <  5:426923/138‑1 (MQ=255)
                              aTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTg                                                                                                         <  1:17947/138‑1 (MQ=255)
                                            cgCCGTAGAAGGATGAACGCGTTATATCGCTTTGCTCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  7:400370/139‑1 (MQ=255)
                                            cgCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  6:214756/137‑1 (MQ=255)
                                            cgCCGTAGAAGGATGAACGCGTTATATCGCTTGGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCCGCGa                                                                                         <  7:97266/139‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  4:260215/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  5:858608/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  3:27614/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  7:234714/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  7:326913/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑CGGC‑GGTTGG‑CCGGCCGCGCGATATTTGTTTATTA‑CGTGGTCTGGACGGCAGc                                                                                           >  2:669369/1‑138 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTGTACTGATGATCAGCGCCTTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  3:643465/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGGCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  2:755982/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCGTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGc                                                                                           <  2:21365/138‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGATTTTCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑GCGCCCGCTCGAT‑TTTTTTTTCTACCGTGGTCTGAACGGCAGCGa                                                                                         <  8:470687/140‑1 (MQ=255)
                                              ccGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  5:810448/138‑1 (MQ=255)
                                              ccGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                         <  3:594468/138‑1 (MQ=255)
                                                 tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGT‑CCGCCCCCGCGAA‑TTTTTTTTATAACGGTGTCTGAAAGTCATCGactcat                                                                                    >  8:605152/1‑135 (MQ=255)
                                                 tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGGTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                    <  7:786308/140‑1 (MQ=255)
                                                  aGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAAGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCt                                                                                     <  4:878865/138‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCTAAGCTTCTg                                                                                 <  4:60312/138‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                 <  5:107649/138‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                 <  5:120810/138‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                 <  4:178353/138‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                 <  4:366765/138‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTg                                                                                 <  6:836464/138‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                               <  7:159070/140‑1 (MQ=255)
                                                           aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GTTTTG‑CCGCCCGCGCGAG‑TTTTTTTTGTAGCGTGGGCTGAACGTCAGCGCAGCGTCTGttagggt                                                                          >  8:206767/1‑134 (MQ=255)
                                                           aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTGGCCCGCCCG‑GCGCT‑TTTGTTTTTTACCGGGGGGTGAAAGTCAGCGAAGGGTGTGTAcatgt                                                                          >  8:744988/1‑136 (MQ=255)
                                                             cgcgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTGG‑GCGCCCGCGGGAG‑TTTTTTTTGTTACGTGGTGTGAAAGTCAGCGCAGCTTATGTACTTGt                                                                          >  2:367941/1‑138 (MQ=255)
                                                                 ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcg                                                                      <  4:237341/138‑1 (MQ=255)
                                                                 ttATATCGCTTTGCGCGGGAAATGTCGCGGAGCCAGGTGCTTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TGTGTTTTCTACCGTTGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcg                                                                    <  7:317580/140‑1 (MQ=255)
                                                                   atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgcg                                                                  <  8:260912/140‑1 (MQ=255)
                                                                   atatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcg                                                                    <  6:82225/138‑1 (MQ=255)
                                                                    tatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcgc                                                                   <  1:367941/138‑1 (MQ=255)
                                                                       cGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                              <  7:101202/140‑1 (MQ=255)
                                                                         cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GTTTGG‑GCGGCCGCGCGCT‑TTTTTTTTTTACCGGGGTGGGAAACGCAGCGCAGCGTGGGTTCTGGTTAgacgcgggggcg                                                            >  8:696347/1‑134 (MQ=255)
                                                                          tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTCCTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTcc                                                             <  3:473104/138‑1 (MQ=255)
                                                                           ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGG‑CCGCCCGCTCGAT‑TTTGTTTTGTAACGTGGTCTGAACGGCAGCGAAGCGTAGGTACTGGTTTCgcgcgcggacg                                                            >  4:887891/1‑133 (MQ=255)
                                                                            tGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGG‑GGTTGG‑GCGCCCCCGCGAA‑TTTTTTTTTAACCGGGGGGGGAACCTCAGCGAACCGTCTGTGCTGGTTAACCGCGCTGCCgc                                                           >  2:248941/1‑137 (MQ=255)
                                                                                        gTCGCTGCGCCATGTGCGTTTTACTGATGATCAGCGCCTTC‑GGGA‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCATCGAAGCTTCTGTACTGGTTACGCTCGATTCCGATCTCAATCCGCt                                               <  5:45999/138‑1 (MQ=255)
                                                                                           gCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACt                                            <  1:436867/138‑1 (MQ=255)
                                                                                           gCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACt                                            <  3:815266/138‑1 (MQ=255)
                                                                                                  cAGGTGTGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GGTTGG‑CCTCTCGCTCGAT‑TTTGTTTTCTACTGTTGTCTTATATTCAGCGAAGCTTCTGTACTTGTTACGCGCGCTTCCTATCTCAATCCTCTAATAGTTTaa                                     <  2:481112/138‑1 (MQ=255)
                                                                                                    ggTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                                   <  6:66544/138‑1 (MQ=255)
                                                                                                           tttACTGATGCTCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGATTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTccc                            <  6:54149/138‑1 (MQ=255)
                                                                                                           tttACTGATGAGCAGCGCCGTC‑GGGC‑GTGTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCCGTccc                            <  2:460275/138‑1 (MQ=255)
                                                                                                            ttACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAA‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCg                           <  5:71475/138‑1 (MQ=255)
                                                                                                              aCTGATGATCAGCGCCGTC‑GTGC‑GTTTGG‑CCGCCCGCGCGAT‑TTTGTATTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                         <  2:324706/138‑1 (MQ=255)
                                                                                                              aCTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                         <  2:780096/138‑1 (MQ=255)
                                                                                                                tgatgaTCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCaa                       <  4:421728/138‑1 (MQ=255)
                                                                                                                tgatgaTCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCaa                       <  1:50555/138‑1 (MQ=255)
                                                                                                                  atgatCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                     <  4:639775/138‑1 (MQ=255)
                                                                                                                        cgcgcCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata               <  6:916029/137‑1 (MQ=255)
                                                                                                                        aGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa             <  8:50953/140‑1 (MQ=255)
                                                                                                                          cgcCGTC‑GGGC‑GGTTGG‑CCGCCCGCTCGAT‑TTTGTTTTGTACCGGGGTGTGAACGTCAGCCAAGCTGGTGGTATTGTTACGCGGGCGTACGAGCCACATCCGCGTCTCGGTGAATTGAGTACCCGCAAGCCTGataaata           >  8:132132/1‑140 (MQ=255)
                                                                                                                           gcCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGGT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaat            <  1:823815/138‑1 (MQ=255)
                                                                                                                           gcCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTTGTCTGAACGTCTGCGAAGCTTCTGTACTGGTTACGAGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataatt            <  1:125107/138‑3 (MQ=255)
                                                                                                                           gcCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaat            <  5:356122/138‑1 (MQ=255)
                                                                                                                            ccGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaata           <  5:386036/138‑1 (MQ=255)
                                                                                                                             cGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa          <  1:390318/138‑1 (MQ=255)
                                                                                                                             cGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg        <  7:744988/140‑1 (MQ=255)
                                                                                                                                    gC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATg    <  1:261789/138‑1 (MQ=255)
                                                                                                                                    gC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATg    <  3:755046/138‑1 (MQ=255)
                                                                                                                                    gC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCATTCCCGGCAAGCCTGATAAATAAGGTATGTc  <  7:180413/140‑1 (MQ=255)
                                                                                                                                       gTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCAATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc  <  1:284488/138‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
ACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTC‑GGGC‑GTTTGG‑CCGCCCGCTCGAT‑TTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTC  >  NZ_CP022686/3734233‑3734503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A