Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F35 I0 R1 271 8871.0 10354886 98.4% 10189207 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,734,367 T→G 22.9% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,734,3670TG22.9% 199.9 / 31.4 151F225V (TTT→GTT) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (15/99);  new base G (35/2);  total (50/101)
Fisher's exact test for biased strand distribution p-value = 4.65e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

ACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAG  >  NZ_CP022686/3734233‑3734505
                                                                                                                                        |                                                                                                                                           
accacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTg                                                                                                                                         >  1:558116/1‑138 (MQ=255)
accacAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTg                                                                                                                                         <  2:558116/138‑1 (MQ=255)
    caGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGgccgcc                                                                                                                                   <  1:582343/140‑1 (MQ=255)
     aGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑CGGGGG‑TTGGgcgccc                                                                                                                                  >  3:678174/1‑140 (MQ=255)
     aGCGGCCCGGTCATTATGGCGGGAGATGTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAGATGTCGCTGCGCCAGGGGCGTTTTACTGATGATCAGCGCCGT‑G‑GGGGGT‑GTGGccgccc                                                                                                                                  >  2:309534/1‑140 (MQ=255)
       cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑G‑GGGGGG‑GTGGgcgcccgc                                                                                                                                >  8:267481/1‑140 (MQ=255)
       cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGT‑TTGGgcgccccc                                                                                                                                >  4:864949/1‑138 (MQ=255)
       cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑GTGGgcgcccgg                                                                                                                                >  8:471811/1‑139 (MQ=255)
       cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGAGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGccgcccgc                                                                                                                                >  6:1034405/1‑140 (MQ=255)
       cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTCTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGgcgccccc                                                                                                                                >  8:397324/1‑138 (MQ=255)
       cGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGAGTTATATCGCTTTGCGCGGGAAATGTCGCTGCTCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑CGGCGTATTGgcaccacc                                                                                                                                 >  3:186626/1‑134 (MQ=255)
        ggCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCt                                                                                                                               <  5:332110/140‑1 (MQ=255)
         gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTc                                                                                                                              <  3:642596/140‑1 (MQ=255)
          cccGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGG‑TTGGccccccgcggg                                                                                                                             >  4:1219332/1‑137 (MQ=255)
          cccGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGG‑TTGGCCGCCcgcgcg                                                                                                                             >  4:494057/1‑140 (MQ=255)
          cccGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGG‑GTGTCCGcgcgcgcg                                                                                                                             >  7:825906/1‑140 (MQ=255)
            cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGTCC‑GGGGGT‑TTGCcccccccgcgaa                                                                                                                            >  7:939285/1‑134 (MQ=255)
            cGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGG‑TTGGCCGCCCGCGCGAt                                                                                                                           >  4:1013412/1‑140 (MQ=255)
              gTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGG‑TTTGCCGCCCGCGCGAatt                                                                                                                         >  1:1017811/1‑140 (MQ=255)
                   tATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGTACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGG‑GTTGCCCCCCCCGCGAGTtttttt                                                                                                                    >  1:516582/1‑140 (MQ=255)
                   tATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑TTGGGCCCCCGCGCGATTGTGttt                                                                                                                    >  7:4520/1‑140 (MQ=255)
                    aTGTCGGTAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGAGTTATATCGCTTTGCGCGGGAAATTTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGtttt                                                                                                                   <  4:755027/140‑1 (MQ=255)
                    aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGtttt                                                                                                                   <  4:1227120/140‑1 (MQ=255)
                        cGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑CGGGGGGT‑TTGCCCGCCC‑CGAGAATTTGTTTTCTaa                                                                                                               >  6:687315/1‑139 (MQ=255)
                        cGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGT‑TTGGGCGCCCCCGCCATTTTGTTTTCTaa                                                                                                               >  2:257978/1‑139 (MQ=255)
                        cGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑GTGGGCGCCCGCGCGATTTTGTTTTTTaa                                                                                                               >  8:66339/1‑139 (MQ=255)
                         gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑TGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                              <  6:927168/140‑1 (MQ=255)
                         gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                              <  7:66339/140‑1 (MQ=255)
                         gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTAcc                                                                                                              <  6:1095779/140‑1 (MQ=255)
                          ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCg                                                                                                             <  3:8736/140‑1 (MQ=255)
                          ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCg                                                                                                             <  6:507503/140‑1 (MQ=255)
                          ggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCg                                                                                                             <  2:188587/140‑1 (MQ=255)
                            agaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTg                                                                                                           <  1:232125/140‑1 (MQ=255)
                                 tCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTg                                                                                                      <  3:620868/140‑1 (MQ=255)
                                            cgCCGTAGTAGGTTGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCTGCGCGGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGTACGTCAGCGa                                                                                           <  7:899520/139‑1 (MQ=255)
                                            cgCCGTAGAAGGCTGAACGCGTTATCTCGCTTTGCGCGGGCAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCAGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  2:1220217/139‑1 (MQ=255)
                                            cgCCGTAGAAGGATGAACGCGTTATATCGCTGTGCGCGGGAAATGTCGCTGCGCCAGGTGCGGTTTACTGATGATCAGCGCCGT‑A‑GGGCGT‑TTGGCAGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  2:451007/139‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATCTCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  5:1179521/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  4:8803/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  5:48821/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  1:295636/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  8:878586/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  7:415944/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  2:931587/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  2:1192796/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  2:1189213/140‑1 (MQ=255)
                                            aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGa                                                                                           <  1:897507/140‑1 (MQ=255)
                                              ccGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCTGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAg                                                                                         <  6:533775/140‑1 (MQ=255)
                                                 tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑TTTGGCGCCCGGAGGCTTTTTTTTTTTACCGGGGTGTGAACGTGAGCGAAGcgg                                                                                      >  8:467279/1‑138 (MQ=255)
                                                 tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑TTGGGCGCCCCCGCGCTTTTTTTTTATACAGTGGTGTGTACGTAAGCCAAGcat                                                                                      >  1:179912/1‑138 (MQ=255)
                                                 tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCtt                                                                                      <  1:363699/140‑1 (MQ=255)
                                                 tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCCG‑GATGCCGCCCCCGCGCTTTTTTTTTATACACTGGGATGAAAGTCAGCGCAGcat                                                                                      >  3:828088/1‑138 (MQ=255)
                                                      ggaggcaCGCGATATATCGCTTTGTTAGGGTAATGTCGGCGCGCCCGGTGCGTTTGAGTGCTGTTGAGGGCCGG‑C‑GGTCGT‑TTTGCCGCCCGCTCGATTTTGTTTTCTACCGGTGTCTGATCGTCAGCGATTCTTCTGtt                                                                                 <  8:1175447/134‑2 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTTCGCGGGAATTGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCTCCGT‑C‑GTGAGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  7:136507/140‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGATATGTCGCTGCGCCAGGTGAGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTATCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  6:868593/140‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  8:213869/140‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  3:386110/140‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  2:1278307/140‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  6:573537/140‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTa                                                                                 <  5:434458/140‑1 (MQ=255)
                                                      ggATGAACGCGTTATATCGCTTTGAGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTTGTCTGAACGTCAGCGTAGCTTCTGTa                                                                                 <  2:822875/140‑1 (MQ=255)
                                                         tGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGG‑TTGGCCGCCCGCGCGATTTTGTTTTATACAGGGGTGTGACCGGAAGCGAAGCTTGTTTAcag                                                                              >  4:810936/1‑138 (MQ=255)
                                                          gAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑TTGGGCGGCCGCGCGCTTTTTTTTTTTTCCGGGGTGTGTACCTCAGCGACTCTTATGTAATgg                                                                             >  5:364702/1‑140 (MQ=255)
                                                           aaCGCGTTATATCGCTTTGCGCGTGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑GTTGCCGACCGCGCGATTTTGTTTTATAAACAGGTCTTAAACTCAGCGAAGGATCTGTTCAGGt                                                                            >  4:590011/1‑140 (MQ=255)
                                                           aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGT‑TTTGGCGCCCCCGCGGTTTTTTTTTTTAACGGGGTCTGGACCTCAGCCACGCTTATGTTATGGt                                                                            >  1:321257/1‑140 (MQ=255)
                                                           aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑GTGGGCGCCCGCGGGCGTTTTTTTTGTTCCGGGGGGGGGACGGCAGCGAAGCTTCTGttgtggg                                                                            >  3:1160161/1‑134 (MQ=255)
                                                                 ttATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTAcgcg                                                                      <  8:1189691/140‑1 (MQ=255)
                                                                         cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑TTGGGCGCCCGCTCGCTTTTTTTTTGTACCGTGGTGTGAAAGTCAGCGCAGCTTCTGTACTGGTTAAGCGCGGGTCCg                                                              >  2:59048/1‑140 (MQ=255)
                                                                         cTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑TTGGGCGCCCGCGCGAGTTTGTTTTTTTCCGGGGGGTGAACGGCACCGCAGCGTCGGTTAGGGTTTAgcgcgcggccg                                                              >  2:905858/1‑135 (MQ=255)
                                                                          tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGG‑TTGGCCGCCCGGGCCATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCGTGAGTACTGGTTTCGCGCGCTAccca                                                             >  6:72408/1‑138 (MQ=255)
                                                                          tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGG‑TTGGCCGCCCGCGCGATTTTGTTTTCTTCCGTGGTCTGCACGACAGAGAAGCATCTGTGATGGTTAAGCGCGCtgacga                                                             >  6:542912/1‑135 (MQ=255)
                                                                               gcgGGTAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACAGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                                        <  7:168196/140‑1 (MQ=255)
                                                                               gcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                                        <  3:622957/140‑1 (MQ=255)
                                                                                        tacccTGCGCCAGGTGCGTTTTACTGATGATAAGCGCCTT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTTGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCAAc                                               <  6:953684/136‑1 (MQ=255)
                                                                                        gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                               <  8:761756/140‑1 (MQ=255)
                                                                                        gTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTAc                                               <  2:1114862/140‑1 (MQ=255)
                                                                                             tGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGGGG‑GTGGGCGCCCGGGCGATTTTTTTTTGTACAGTGGTCGGGACGTCAGCGCAGCGTATGTACTTGTTACCCGCGCGGCCGATCACAAAACGCGACTCggg                                          >  4:63689/1‑138 (MQ=255)
                                                                                                           tttACTGATTATCAGCGCCGT‑C‑GGGCTG‑TTGGACTCACGCTCTATTTTGTTTTCTACAGTGGTCTGAAAGTCAGAGAAGCTTCTGTACTGGTTACGCTCGCTTCCTATCACAATCCGCTACTCGTTGAATTCAGTCCAgg                            <  4:264912/140‑1 (MQ=255)
                                                                                                              aCTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCaa                         <  6:353545/140‑1 (MQ=255)
                                                                                                                  atgatCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGAtt                                                                                                                          <  1:782408/39‑1 (MQ=255)
                                                                                                                  atgatCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                     <  6:760477/140‑1 (MQ=255)
                                                                                                                  atgatCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                     <  5:72408/140‑1 (MQ=255)
                                                                                                                  atgatCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt                     <  2:1153227/140‑1 (MQ=255)
                                                                                                                  atgatCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCGCGAtt                                                                                                                          >  2:782408/1‑39 (MQ=255)
                                                                                                                   tgatCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                    <  8:739633/140‑1 (MQ=255)
                                                                                                                   tgatCAGCGCCGG‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                    <  3:1193773/140‑1 (MQ=255)
                                                                                                                   tgatCAGAGCCGT‑G‑TGGCGG‑TTGGCCGCACGCTCGATTGTGTTTTCTAGCGGGGTCTGATCGTCAGCGACGCGTCTGGACTGGTTACGCGCGCTTCCGATCTCTATCCGGTACTCGGTGAATGCTGTCCCGGCAAGccgg                    <  4:1141269/140‑3 (MQ=255)
                                                                                                                      tCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata                 <  8:384244/140‑1 (MQ=255)
                                                                                                                        cgcgcCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTGTTCTACCGTGTTCTGAACGTCAGCGAAGCTTCTGTACTGGGTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  1:781145/139‑1 (MQ=255)
                                                                                                                        aGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  1:915132/140‑1 (MQ=255)
                                                                                                                        aGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaa               <  3:148478/140‑1 (MQ=255)
                                                                                                                           gcCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  8:620531/140‑1 (MQ=255)
                                                                                                                           gcCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa            <  3:426426/140‑1 (MQ=255)
                                                                                                                              cggc‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg          <  5:526930/137‑1 (MQ=255)
                                                                                                                                     gCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc    <  4:458737/140‑1 (MQ=255)
                                                                                                                                     gCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTc    <  1:1124405/140‑1 (MQ=255)
                                                                                                                                     gCGT‑TTGGCAGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGTTATTTc    <  5:1096193/140‑1 (MQ=255)
                                                                                                                                     gCGT‑GTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGACCGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCTATCACAATCCGCTACTCGTTGATTTCAGTATATGAAAGCATGATAAATAAGGTATGTc    <  2:1084026/140‑1 (MQ=255)
                                                                                                                                       gT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAg  <  1:999938/140‑1 (MQ=255)
                                                                                                                                       gT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGGTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAg  <  2:442787/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
ACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACTGATGATCAGCGCCGT‑C‑GGGCGT‑TTGGCCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGCGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAG  >  NZ_CP022686/3734233‑3734505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A