Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F13 I0 R1 150 9496.0 7621532 98.4% 7499587 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,633,846 T→G 27.4% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,633,8460TG27.4% 207.1 / 45.8 169N253T (AAC→ACC) yqeBselenium‑dependent molybdenum cofactor biosynthesis protein YqeB
Reads supporting (aligned to +/- strand):  ref base T (33/86);  new base G (47/2);  total (81/88)
Fisher's exact test for biased strand distribution p-value = 1.86e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

TCCAACATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGC  >  NZ_CP022686/1633711‑1633981
                                                                                                                                       |                                                                                                                                        
tCCAACATGATCACTTTAAAACCCTCATGATGCAGACGTAGCGCTACACCACTTGCGATATCCCCCGCACCGCGAATCACCACCAGCTGTTGACCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCTGCGCGTTAtt                                                                                                                                      >  1:947809/1‑140 (MQ=255)
   aaCATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCATCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTAttttt                                                                                                                                   <  1:403048/140‑1 (MQ=255)
      aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAc                                                                                                                                <  1:945614/140‑1 (MQ=255)
      aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAc                                                                                                                                <  7:394156/140‑1 (MQ=255)
      aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAc                                                                                                                                <  4:406066/140‑1 (MQ=255)
       tGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAcc                                                                                                                               <  7:895247/140‑1 (MQ=255)
         aTCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAAccag                                                                                                                             >  1:895558/1‑138 (MQ=255)
           cACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCACCTgaa                                                                                                                           >  4:143992/1‑138 (MQ=255)
               ttAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGCAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑Aatat                                                                                                                      <  3:245542/140‑1 (MQ=255)
                     cccGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTGCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGc                                                                                                                <  4:369430/140‑1 (MQ=255)
                     cccACATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGA‑AATATTTCTGc                                                                                                                <  2:350082/140‑1 (MQ=255)
                        gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAg                                                                                                             <  8:402757/140‑1 (MQ=255)
                        gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGAACAGT‑AATATTTATTCCac                                                                                                             >  3:587324/1‑139 (MQ=255)
                        gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACCTGT‑AATATTTCTGCCaa                                                                                                             >  2:20093/1‑139 (MQ=255)
                        gCATGATACAGACGTAGCGCTACACCACTGGAGATATCCCCCGCACCGCGAATCACCACCAGCTTGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAg                                                                                                             <  7:211144/140‑1 (MQ=255)
                        gCATCATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACAGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAg                                                                                                             <  4:189757/140‑1 (MQ=255)
                        ccATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAg                                                                                                             <  6:473529/139‑1 (MQ=255)
                                cAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTaa                                                                                                     <  4:264514/140‑1 (MQ=255)
                                cAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAACCTGT‑AATATTTATGCCAGCACGCTaa                                                                                                     >  8:789176/1‑140 (MQ=255)
                                     gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCg                                                                                                <  8:690315/140‑1 (MQ=255)
                                     gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCg                                                                                                <  1:315842/140‑1 (MQ=255)
                                     cgaGCGCTACACCACTGGCGATATACCCCGCACCGCGAATCACCACCAGCTTGTGTCCGGAACGATGAGAATGCCTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCg                                                                                                <  2:515955/138‑1 (MQ=255)
                                      tAGCGCTACACCACTGGCGATATCCCCCGCACCGCGCATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTGTTTTTTCACCGGG‑AATATTTATGCCAGCCCGCGAATggggg                                                                                               >  8:826084/1‑137 (MQ=255)
                                      tAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTTTTTTTTACCTGT‑TATATTTCTGCCAGCACGCGAATGGCgg                                                                                               >  8:198204/1‑139 (MQ=255)
                                       aGCGATACACAACTGTCGATATCCCCCGCACCGCGAATAAGCCCCAGCTCTTGTCCGGAAGGATGAGAAGGCTTCATCATCAAACCCACCGGCGATTGATTTTTCACCGCTAAATATACATG‑CAGCACGCCAATGGCgct                                                                                              >  2:516216/1‑138 (MQ=255)
                                        gcgcTACACCCCTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTACATCATCAACCCACCCGGCGCGGTTTTTTTAACCAGT‑AATATTTCAGCCAGCACCCAAATTGCgcta                                                                                             >  4:644593/1‑137 (MQ=255)
                                        gcgcTACACCACTGGCGATATTCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTTCCAGCACGCTAATGGCGAtc                                                                                             >  4:83546/1‑140 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCCCCAGCTGGTGTCCGGAAGGCTGAGAAGGCTTCATCCTCAACCCACCCGGCGCGGTATTTTTGCCCAGG‑AATATTTCTCCCCGGACCCTAATggggcac                                                                                             >  7:676312/1‑135 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTTTTTTTTCCCGGT‑AATATTTCTGGCAGCCCGGGAATgggggta                                                                                             >  3:598056/1‑135 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGCGGTTTTTTTGAACCGG‑AATATTTTTGGCAGCACGCCAATGGCggac                                                                                             >  1:661361/1‑137 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTAttttt                                                                                                                                   <  7:411249/103‑1 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTAttttt                                                                                                                                   >  8:411249/1‑103 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACCTGT‑AATATTTATGCCAGCAAGGTAATGGCGAta                                                                                             >  2:575921/1‑139 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGAACTGT‑AATATTTCTGCACGCACGCTAATGGCGAtc                                                                                             >  4:738374/1‑140 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTGTTTTTTAAACAGG‑AATATTTATGACCGCACGCTAAAGGCgttg                                                                                             >  1:748844/1‑137 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTTAACAGG‑TATATTTATGCCAGCACGCGAATGGCGCtc                                                                                             >  3:795485/1‑140 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGTTTTTTAACCGGG‑TATAATTATTGCAGCACCCGAATGGCgcac                                                                                             >  2:840507/1‑137 (MQ=255)
                                        gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAACGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCAGCTGT‑AAATTTTCTGCCACCCAGCGAATGGGGAtg                                                                                             >  1:229821/1‑139 (MQ=255)
                                             acacCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGGATTTTTGACCTGT‑TATTTTTATGCCAGCCCCCGAATGGCGGTGTccgg                                                                                        >  6:40930/1‑138 (MQ=255)
                                             acacCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTg                                                                                        >  4:954330/1‑140 (MQ=255)
                                                ccacTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTgcgg                                                                                     <  4:437281/140‑1 (MQ=255)
                                                ccacTGGAGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTgcgg                                                                                     <  1:231185/140‑1 (MQ=255)
                                                   cTGGCGATATCCCCCGGACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCAGCAACCCCCCCGGCGCGTGATTTTTCACCTGT‑ATTATTTCTGGCCGCCCGCAAAAGGAGCTCGACTgcggcgg                                                                                  >  6:383877/1‑139 (MQ=255)
                                                   cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATTAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCGGT‑AATATTTATGCCGACACGCTAATGGCGATCTCCTGCGGCGt                                                                                  >  4:384631/1‑140 (MQ=255)
                                                   cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGCGGTTTTTTTGACCTGT‑TATATTTATGGCAGCACGCGAATTGCGATATCCAgcggcgg                                                                                  >  5:417879/1‑139 (MQ=255)
                                                   cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTGACCAGT‑AATATTTATGCCAGCCCGCTAATGGCGCTCTCCTgcggcgg                                                                                  >  7:166681/1‑139 (MQ=255)
                                                   cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGt                                                                                  >  4:720621/1‑140 (MQ=255)
                                                   cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTTACCTGT‑TATATTTATTCCAGCCCGCTAAAGGCGCTGTCCTtcggcgg                                                                                  >  4:475904/1‑139 (MQ=255)
                                                   cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAAACAGT‑TATATTTAATCCAGCACGCTAATTGGGATCTACTGCGGCGt                                                                                  >  1:794173/1‑140 (MQ=255)
                                                   cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGT‑TTTTTCACCGGT‑TATATTTGTGCCAGCACGCTAATGGGGAACTCCTGCGGCGtt                                                                                 >  2:291363/1‑140 (MQ=255)
                                                      gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTGAACTGT‑AATATTTATGCCCGCACGCTAATGGCGATCTACATCGGCGgttg                                                                               >  1:707418/1‑139 (MQ=255)
                                                      gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                               <  3:901074/140‑1 (MQ=255)
                                                      gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                               <  2:640787/140‑1 (MQ=255)
                                                      gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACCAGT‑TAAATTTTTGCCCCCACGCAAAAGGCGATCACCATCGGCGtttg                                                                               >  2:114574/1‑139 (MQ=255)
                                                      gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGGGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                               <  1:469264/140‑1 (MQ=255)
                                                      gCGATATCCCCCGCACCGCGAATCACCACCAGCAGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                               <  7:281999/140‑1 (MQ=255)
                                                        gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCg                                                                             <  3:64403/140‑1 (MQ=255)
                                                        gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCg                                                                             <  4:118054/140‑1 (MQ=255)
                                                        gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCg                                                                             <  8:479671/140‑1 (MQ=255)
                                                           atCCCCCGCACCGCGAATCCCCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGCGTTATTTTTAACCTGT‑AATATTTCTGCCAGCACGCTAAAGGCGATCTCCTGCGGAGTTTCCGccc                                                                          >  8:206006/1‑138 (MQ=255)
                                                           atCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCAc                                                                          <  4:852412/140‑1 (MQ=255)
                                                           atCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCGGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCAc                                                                          >  4:837084/1‑140 (MQ=255)
                                                           atCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTCACCTGT‑AATATTTATGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCCCAc                                                                          >  6:590548/1‑140 (MQ=255)
                                                             cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                        <  5:865868/140‑1 (MQ=255)
                                                             cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                        <  7:28712/140‑1 (MQ=255)
                                                             cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                        <  7:4415/140‑1 (MQ=255)
                                                             cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                        <  7:622772/140‑1 (MQ=255)
                                                             cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTGCATCATCACCCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCATGCGGCGTTTCCGCACCt                                                                        <  7:732072/140‑1 (MQ=255)
                                                             cccccGCACCGCGAATCACCACCAGCTGGTGGCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                        <  2:555897/140‑1 (MQ=255)
                                                                ccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTTAACAGT‑AATATTTATTGCACCACCCGAATGGCGATCTACAGCGGCGGTTACGCCCCCAtt                                                                     >  3:270430/1‑139 (MQ=255)
                                                                     ccGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTa                                                                <  4:238227/140‑1 (MQ=255)
                                                                      cgcgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGATTTTTCACCTGT‑AATATTTATGCCAGCCCGCGAATGGCGATCTACAGCGGCGTTTCCGCACCTATTTTGTaa                                                               >  8:836234/1‑140 (MQ=255)
                                                                        cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTAACCAGT‑AATATTTATTCAAGCACACTAATGGGGATCAACTTCAGCGGTTGCGCACCAATGTTTTAAcc                                                             >  7:299807/1‑140 (MQ=255)
                                                                        cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAAcc                                                             >  2:944552/1‑140 (MQ=255)
                                                                        cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTGACCGGT‑AATATTTCTGCCACCACGCGAATGGCGCTCTCCAGCCGGGTTTACGCCCCTATGTTGTTAcc                                                             >  4:310194/1‑140 (MQ=255)
                                                                              accaccATCTGGTGTCCGGAAGGATGAGAAGGCTTAATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACggg                                                       <  7:510425/140‑1 (MQ=255)
                                                                              accaccAGCTGGTGTCCGGACGTATGAGAAGGCGTCATCATCAACCGACTCGGCGCTTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCTATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACggg                                                       <  1:57307/140‑1 (MQ=255)
                                                                              accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACggg                                                       <  8:507779/140‑1 (MQ=255)
                                                                              accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACggg                                                       <  3:818176/140‑1 (MQ=255)
                                                                              accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACggg                                                       <  2:378263/140‑1 (MQ=255)
                                                                                caccaGCTGGTGTCCGGAAGGATGCGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGgcg                                                     <  7:863789/140‑1 (MQ=255)
                                                                                     gCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTCACCGGT‑AATATTTGTGCCCGCACGCTAATGGGGCTCTCCGGCGGCGTGTGCGGACCTATGTTGTTTCCCACGGGGGCGTgg                                                >  2:772437/1‑139 (MQ=255)
                                                                                        ggggtCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGAGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                             <  5:708476/137‑1 (MQ=255)
                                                                                        ggTGTCCGGAATGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTCCCAGCACGCTAATGGCGATCTCCTGCGGCGGTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                             <  4:230904/140‑1 (MQ=255)
                                                                                        ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                             <  3:228592/140‑1 (MQ=255)
                                                                                        ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                             <  1:385409/140‑1 (MQ=255)
                                                                                         gtgtCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAAc                                            <  8:293581/140‑1 (MQ=255)
                                                                                          tgtCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTACTGCGGCGTTTc                                                                               >  8:384465/1‑104 (MQ=255)
                                                                                            tCCGGAAGGATGAGAAGGCTTCATCATGAACCCACCCGGCGCGTTATTTTTCACGTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCAGCTATGTTGTAACCAACGGGCGCATGTAAACggg                                         <  5:420675/140‑1 (MQ=255)
                                                                                            tCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACggg                                         <  8:10471/140‑1 (MQ=255)
                                                                                                 aaGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTTT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTTTGTTGTAACCAACGGGCGCATGTAAACGTGCAata                                    <  2:674510/140‑1 (MQ=255)
                                                                                                 aaGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAata                                    <  3:720621/140‑1 (MQ=255)
                                                                                                    gATGAGAAGGCTTCATCATCAACACACCCGGCGCGGTTAGTTTAACCGGT‑AATATTTATGCCACCACACTTATGGGGCAAGCCTGCGCCGGTTCCGCCCCACTAGTGTATCAAACCGGCGCCTCTAAAACGGCAAAATGt                                 >  6:301138/1‑140 (MQ=255)
                                                                                                      tGAGAAGGCTTCATCATCAACCCACCCGGCGCGTGATTTTTCACCTGT‑AATATTTCTGCCAGCCCGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACAAACGGGCGCATGTAAACGGGCAATATGttc                               <  4:724439/140‑1 (MQ=255)
                                                                                                      tGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAAACTGT‑AATATTTATGCCAGCACGCTAATGGCGATCTCCTGCGGGGTTTCCGCACCAATGTTGGAACCAACGGGCGCATGTAAACGGGCCATATGttg                               >  6:550860/1‑139 (MQ=255)
                                                                                                                 catcatcaACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGc                    <  7:836234/140‑1 (MQ=255)
                                                                                                                 catcatcaACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCAGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTGCTTCAGCCACGc                    <  5:887658/140‑1 (MQ=255)
                                                                                                                     atcaACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCttt                <  7:365224/140‑1 (MQ=255)
                                                                                                                       caACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTATTCCAGCACGCGAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTTTTAACAACGGGCGCATGTTAACCGGCAAAATGTTCTTAAGCCCCGCCGTtcg              >  8:63649/1‑139 (MQ=255)
                                                                                                                              cccGGCGCGTTATTTTTCACCTGT‑AATATTTAAGCCCGCACCCTAATGGCGATATCCAGCGGCGTTTGCGCACCGATTTTGTAAACAACGGGCGCAAGTTAAAAGGCAATAATTTATTAAGCCACCCCATTCTCACGCaa       >  6:857609/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                        
TCCAACATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGT‑AATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGC  >  NZ_CP022686/1633711‑1633981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A