Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F36 I0 R1 107 8366.7 6299831 98.3% 6192733 135.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,633,846 T→G 30.1% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,633,8460TG30.1% 133.7 / 31.6 126N253T (AAC→ACC) yqeBselenium‑dependent molybdenum cofactor biosynthesis protein YqeB
Reads supporting (aligned to +/- strand):  ref base T (29/57);  new base G (38/2);  total (67/59)
Fisher's exact test for biased strand distribution p-value = 2.11e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

ATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGG  >  NZ_CP022686/1633717‑1633980
                                                                                                                                 |                                                                                                                                       
ctGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAc                                                                                                                               <  7:65229/139‑1 (MQ=255)
aTGATCTCTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAc                                                                                                                               <  5:131820/140‑1 (MQ=255)
aTGATCACTTTCAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTc                                                                                                                                 <  3:380025/138‑1 (MQ=255)
aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTc                                                                                                                                 <  3:260895/138‑1 (MQ=255)
aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTc                                                                                                                                 <  3:178895/138‑1 (MQ=255)
aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTc                                                                                                                                 <  4:533033/138‑1 (MQ=255)
aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTc                                                                                                                                 <  4:264973/138‑1 (MQ=255)
aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAc                                                                                                                               <  8:445170/140‑1 (MQ=255)
aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTGCATCATCAACCCACCCGGCGCGTTATTTTTCAc                                                                                                                               <  7:328089/140‑1 (MQ=255)
   aTCACTTTAAAACCCGCATGATACAGACGTAGCTCTACACCACAGGCGATATCCCCCGCACATCCAATCACCCCCAGCTGGTGTCCGGAAGGATGAGAAGTCTTCATCATCTACCCACCCGTCGCGTttttttagaaatg                                                                                                                            >  5:211609/1‑133 (MQ=255)
   aTCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTg                                                                                                                            <  8:120783/140‑1 (MQ=255)
           aaacgccGGATGATACAGCCCTAGCGCTTCCCCACTGGCGATTTCCCCGGCACCGCGAATTTCCTCATGCTGGTGTCCTGAAGGATGAGAAGGCTTCTTCATCAACGCTCGCGGTGCGGTATTTTTCACCTGTAATAttt                                                                                                                    <  8:547633/135‑1 (MQ=255)
            aaaCCCGCATGATACAGACGTAGCGCTACACCACTGGCTATATCCCCCTCACCGAGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCGTCATCATCAACCCACCCGGCGCGTTAATTTTAACCAATAATAtt                                                                                                                     >  2:211820/1‑138 (MQ=255)
                  gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCTCAACGCGAATCCCCACAAGCTGGTGTCCGGCAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGcc                                                                                                               <  3:715589/138‑1 (MQ=255)
                  gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGTTATTTTTTAACGGTAATATTTATGCCac                                                                                                             >  5:540299/1‑139 (MQ=255)
                  gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGcc                                                                                                               <  3:223934/138‑1 (MQ=255)
                  gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGcc                                                                                                               <  4:145333/138‑1 (MQ=255)
                  gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAACCTGTAATAtttattgcac                                                                                                             >  8:461711/1‑133 (MQ=255)
                  gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCAGCTGTAATATTTCTGCCAg                                                                                                             >  8:247828/1‑140 (MQ=255)
                  gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTAATTTTCACCAGGTATAATTCTGCCac                                                                                                             >  8:586408/1‑139 (MQ=255)
                  gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGACCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTAATTTTAAACCGTAAAATTTCTGCCac                                                                                                             >  7:43247/1‑139 (MQ=255)
                  gCATGATACAGACGTAGCGCTACACCACTGGCGATAACCCCCGCACCGCGAATCACCACCAGCTGTTGTCCGGAAGGATGAGAAGGCTTCATCATCATCCCACCCTGCGCGTTATTTTTCACCTGTAATATTTCTGCCAg                                                                                                             <  6:768381/140‑1 (MQ=255)
                  ccATGTTACATACGTATCGCTACACCACTGGGGATATAGCCCGCTACGCGAATCACCAGCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACGCGGCGCCTTATTTTTCACCTGTAATATTTCTGCCAg                                                                                                             <  5:421445/139‑1 (MQ=255)
                  ccATGATACAGACGTCGCGCTACCCCACTGGCGAGATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAg                                                                                                             <  6:698660/139‑1 (MQ=255)
                    aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCCACCCACCCGGCGCGTTTTTTTTCACCAGGAATATTTCTGGCAg                                                                                                             >  4:188944/1‑138 (MQ=255)
                    aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCAGGAATATTTATGCCac                                                                                                             >  3:669544/1‑137 (MQ=255)
                    aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGTTTTTTAACCCGTTATATTTTTGCCAg                                                                                                             >  3:786039/1‑138 (MQ=255)
                          cAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCt                                                                                                       <  4:37647/138‑1 (MQ=255)
                               gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTCACCTGTAATTTTTATTCCAGCACACTAATgg                                                                                                  >  3:800136/1‑138 (MQ=255)
                               gTAGCGCTACACCACTGGAGATATCCGACGCACCGCTAATCACCACTAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGct                                                                                                <  6:537229/140‑2 (MQ=255)
                                  gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCTCCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCAGGCGCGTTATGTTTCAAGTGTAATAATTCTGGCCGCTCGCTAATGGCGAtg                                                                                             >  6:672139/1‑139 (MQ=255)
                                  gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTGACCTGGAATATTTATTCCCGCACCCTGATGGCGAtc                                                                                             >  5:91078/1‑140 (MQ=255)
                                  gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCTCCAGTAATATTTATTCCCGCCCCCGAATGGccaga                                                                                             >  7:262210/1‑136 (MQ=255)
                                    gcTACACCACTGGCGATATCCCCCGCACCGCGACTCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTCACCGGTTTAATTTATGACAGCCCGCGTATGGCGAtg                                                                                             >  2:799055/1‑137 (MQ=255)
                                    gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTTTGTTTTACCGGGTATTTTTATGCCAGCACACGATTGGCgctg                                                                                             >  3:173793/1‑135 (MQ=255)
                                    gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTAACAAGTAATATTTTTGACAGCACCCTAATTGCGAta                                                                                             >  2:724688/1‑137 (MQ=255)
                                    gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACCGGTAATATTTATGCCCGCACGCGAATGGCGAta                                                                                             >  2:276052/1‑137 (MQ=255)
                                    gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACCTGTAATATTTCTTCCAGCACGCTAATTGCgcta                                                                                             >  3:463651/1‑135 (MQ=255)
                                    gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTTACCGGGTATATTTATGACAGCACCGGAATGGCGaac                                                                                             >  2:542102/1‑136 (MQ=255)
                                    gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTTAAATGTAATTTTTCTTGCAGCACGCGAATGGGGAtg                                                                                             >  3:471456/1‑137 (MQ=255)
                                    gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACCGGTAATATTTATGACAGCACGCTAATGGCGAta                                                                                             >  3:230335/1‑137 (MQ=255)
                                       acacCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTATGCCAGCACGCTAATGGCGCTCTCCTg                                                                                        >  8:234100/1‑140 (MQ=255)
                                        caccacTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTGACCTGGTATATTTATGCCAGCACCCTAATGGGGCTCTCCTgc                                                                                       >  5:34064/1‑140 (MQ=255)
                                         accacTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTTACCGGTAATATTTATGCCCGCACGCTAATGGCGATCTACTg                                                                                        >  1:264983/1‑138 (MQ=255)
                                             cTGGCGATATGCCCCGCACCGCGAAGCACCACCCGCTGGGGTCCGGAAGGATGAGAAGGCTTCTTCATCAACCCACCCGGCGCGGTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTgcggc                                                                                    <  2:721792/138‑1 (MQ=255)
                                             cTGGCGATATCCCCCGCACCGCGAATCACCACCCGCTGGTGTCCGGAAGGAGGAGAAGGCTTCATCATCCACCCCCCCGGCGCGGTAGTTTTATCCTGTTATATTTATGCCAGCCCGGTAAAGGCGAGCGCCGTCGGCGt                                                                                  >  8:189814/1‑140 (MQ=255)
                                             cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTATTTTTTTCCAGTTATATTTATGCCCCCACCCGAATTGGGGTCTCCTgcggg                                                                                    >  1:310888/1‑137 (MQ=255)
                                               ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGCGTGATTTTTCACCTTTAATATTTCTGCCATCCCGCGAATGGCGATCTCCGGCGGCGt                                                                                  >  4:586112/1‑138 (MQ=255)
                                               ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACCTGTAATATTTATGGCAGCACGCAAATGGCGATCTCCTgcggcgg                                                                                  >  4:276557/1‑137 (MQ=255)
                                               ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTGACCAGTAAAATTTATGCCAGCACGCGAATGGCGATCTCCAgcggcgg                                                                                  >  2:220485/1‑137 (MQ=255)
                                               ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTCACCAGTTATATTTATGCCCGCACGCTAATGGCGATCTCCTGCGGCGt                                                                                  >  2:413114/1‑138 (MQ=255)
                                               ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGACCTGGATTATTTGTGCCCGCACCCTAAAGGCGATATCCAgcggcgg                                                                                  >  3:746980/1‑137 (MQ=255)
                                               ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCAACCGGCGCGTTATTTTTAACCTGTTATATTTCTGGCAGCACGCTAATGGCGATCAACAGCGGCGt                                                                                  >  4:462907/1‑138 (MQ=255)
                                                gCGATATCCCCCGCCCCGCGAATCACCCCCAGCTGGTGTCCGGAAGGAGGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTAATTTTCACCTGTAATCTTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                               <  8:680269/140‑1 (MQ=255)
                                                gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGtt                                                                                 <  1:326000/138‑1 (MQ=255)
                                                gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTATCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTATTAAACAGAAATATTTATGGCCGCACGCAAATGGCGAAAACCTGCCGCGgtta                                                                               >  6:405273/1‑139 (MQ=255)
                                                  tatatCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGTAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                               <  1:584964/137‑1 (MQ=255)
                                                  gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCTCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                               <  3:113952/138‑1 (MQ=255)
                                                  gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                               <  1:683161/138‑1 (MQ=255)
                                                  gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                               <  3:399581/138‑1 (MQ=255)
                                                  gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCg                                                                             <  7:428986/140‑1 (MQ=255)
                                                  gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTGACCTGGTATATTTGTGGCCGCCCGCTAATGGCGCTCTACTGCGGCGTTTGCg                                                                             >  6:738337/1‑140 (MQ=255)
                                                     atCCCCTGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAACCGGTTATATTTATGGCAACACGCAAATTGGGGTCTACTGCGGGGTTTACCCAc                                                                          >  7:404898/1‑140 (MQ=255)
                                                     atCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGc                                                                            <  1:364935/138‑1 (MQ=255)
                                                       cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCAc                                                                          <  4:293304/138‑1 (MQ=255)
                                                       cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCAc                                                                          <  4:372128/138‑1 (MQ=255)
                                                       cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACCTGTAATATATATGCCAGCACGCGAATGGCGATCTCCTGCGGCGTTTCCGCAc                                                                          >  4:715017/1‑138 (MQ=255)
                                                       cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGTTTTTTTGCCTGGTATATTTTTTGCCGCACGCGTATGGGGTTCTTCGGGGGGGGTTCCGCAc                                                                          >  1:729871/1‑138 (MQ=255)
                                                       cccccGCACCGCGAATCACCACCAGCTGGTATCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑t                                                                       <  5:405273/140‑1 (MQ=255)
                                                       cccccGCACCGCGAATCACCACCAGCGGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACCTGTAATATTTCTGCCCGCCCGCGAATGCCGATCTCCAGCGGCGGTTACGccc                                                                          >  4:698655/1‑136 (MQ=255)
                                                            gCACCGCGAATCACCCCCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCATCCCACCCGGCGCGTTATTTTTCACAAGTTATATTTATTCCAGCCCGCGAATGGCGCTCTCCAGCGGCGTTTGCCCACC‑TAtt                                                                    >  2:364935/1‑137 (MQ=255)
                                                             cACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TAtgttg                                                                 <  6:656238/140‑1 (MQ=255)
                                                              aCCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTGACCAGTTATATTTATGCCAGCACCCGGATTGGGATCAGCAGCGGCGGGTGCGCACC‑GAtttt                                                                  >  3:136035/1‑138 (MQ=255)
                                                               ccGCGACTCCCCACCCGCAGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGTCGCGTTATTTTTCACCTGTATTATTTCAGCCTGCACGCTAATGGCGATCTCCTGCTGCGTTTCCGCACC‑TAtgttg                                                                 <  1:69484/138‑1 (MQ=255)
                                                                cgcgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAAACTGTTATATTTATTCCACCACGCGAATTGGGCTCTACAGCGGCGTTTGCGCACC‑AAtttttt                                                                >  2:733502/1‑136 (MQ=255)
                                                                  cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTATTTTTACCCTGTAATAGTTCTGCCACCCCGCGAAAGGCGCACTCCAGCGGCGGTTACCCCCA‑AATGTTGTAAcc                                                            >  6:335368/1‑140 (MQ=255)
                                                                  cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACCGGTAATATTTATGGCAGCACGCGAATGGCGATCTCCTGCGGCGGTTACGCACC‑TATGTTGTAAcc                                                            >  6:442969/1‑140 (MQ=255)
                                                                  cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCGTCATCATCAACCCACCCGGCGCGGTATTTTTGACCTGTAATATTTCATCCACCCCCCGAATGGCGAGATCCGTCGGCGTTTGCGCACC‑AATGTTGTAGcc                                                            >  6:209942/1‑140 (MQ=255)
                                                                   gAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGATTCCGCACC‑TATGTTGTAAc                                                             <  4:141775/138‑1 (MQ=255)
                                                                        accaccAGCTGGTGTCCGGAAGGATGAGAAGTCTTCATCATCAACCCACCAGGGGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACg                                                        <  4:718083/138‑1 (MQ=255)
                                                                        accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACg                                                        <  2:483610/138‑1 (MQ=255)
                                                                               gCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACCAGTTATATTTATTCCCGCACCCGAATTGGGGACTCCAGCGGGGTTTCCGCACCATAT‑TTGTAACCAACGGGCGCCTgg                                               >  8:261583/1‑139 (MQ=255)
                                                                                  ggTGTCCGTAATGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGGAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACGGGCGCATGTaaa                                            <  8:369984/140‑1 (MQ=255)
                                                                                  ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACGGGCGCATGTaaa                                            <  8:26996/140‑1 (MQ=255)
                                                                                  ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACGGGCGCATGTaaa                                            <  7:495790/140‑1 (MQ=255)
                                                                                  ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACGGGCGCATGTaaa                                            <  5:507377/140‑1 (MQ=255)
                                                                                  ggTGGCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACGGGCGCATGTa                                              <  3:75642/138‑1 (MQ=255)
                                                                                      tCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTAACCTGTAATATTTATTCCAGCACCCTAATGGCGAACTCCTGCGGCGTTTCCGCCCC‑GATGTTGTAACCAACGGGCGCAAGGAAACg                                          >  4:85698/1‑138 (MQ=255)
                                                                                          gAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGAACGGTAATATATATGACAGCACGCAAATTGGGTTCTACTGCGGCCTTTCCGCACC‑AAAGTTGTTAACAACGGGCGCATGTAAACCGGCa                                      >  4:544270/1‑138 (MQ=255)
                                                                                           aaGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATATCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACGGGCGCATGTAAACGGGCAata                                   <  8:678016/140‑1 (MQ=255)
                                                                                               aTGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTGACCGGTTATATTTCTGCCCGCACGCTAATGGCGATATACAGCGGCGTTTCCGCACC‑AATGTTGTTAACAACGGGCGCCTGTAAAAGGGCAatatt                                 >  2:38553/1‑137 (MQ=255)
                                                                                                tGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACCGGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGttg                              >  8:623895/1‑139 (MQ=255)
                                                                                                           catcatcaACCCACCCGGCGCGTTATTTTTAACCTGTTATATTTATGCCAGCCCCCGAATGGCGGTCTCCAGCGGGGTTGACGCACC‑TATGTTTGAACCCACGTGGGCATTTTAACGGGGAATAAGTTGATGAGCCAc                     >  2:521562/1‑138 (MQ=255)
                                                                                                           catcatcaACCCACCCGGCGCGTTATTTTTAACCGGGTATATTTATGCCCGCCCGCGTATTGCGTGCTCCTGCGGCGGTTGCGCACC‑CAAGTTGGAACCCACGGGCGCATGGAAACGGGGAATAATTTGTTGAGcccc                     >  2:321736/1‑136 (MQ=255)
                                                                                                             tcatcaACCCACCCGGCGCGTTATTTTTAACCTGTAATATTTATGCCAGCACGATAATGGCGATCTCCAGCGGCGTTTACGCACC‑AATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATTTTATTCAGCCACGc                   >  2:216143/1‑138 (MQ=255)
                                                                                                                  aaCCCACCCGGCGCGTTATTTTTGACCTGTTATATTTCTGCCCGCACGCTAATGGCGATATCCAGCGGCGGTTCCGCACA‑AATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTATTCAGCCACGCCGTtctg            >  8:6507/1‑139 (MQ=255)
                                                                                                                   acccacccGGCGCGTTATTTTTAACCTGGAATATTTGTTCCCGCACGCGAATGGGGATCTACTGCGGCGGTTGCGGCCC‑TATTTTGTACCCCACCGGCGGAGGTAAAAGGGCTATATGTTGTTCCGCCCCGCCGTtatcc           >  8:419721/1‑139 (MQ=255)
                                                                                                                        cccGGCGCGTTTTTTTTAACCTGTAATATTTATGCCAGCACGCTAATGGCGCTATACTGCGGGGTTTGCGCCCC‑TATTTTTTAACCAACGGGCGCCTGTAAACGGGCCATATTTTCTTAAGCCCCGCCATTGTACCGCaa      >  6:336768/1‑140 (MQ=255)
                                                                                                                            gcgcGTTATTTTTAACCTGTAATATTTATGCCCGCCCGCTAATGGCGATCTCCAGCGGCGTTTCCGCACC‑GATGTTGTAACCAACCGGCGCATGTAAACGGGCAATAAGTTCTTCAGCCACCCCATTCTCCCGCaaattg  >  8:675176/1‑136 (MQ=255)
                                                                                                                             cgcgTTATTTTTCACCTGTAATATTTCTGCCAGCACTCTAATGGCTATCTCCTGCGGCGTTTCCGCACG‑TATGTTGTAACCAACGGGCGCATGTAAACGGGGAATATGTTCTTCAGCCAAGCCTTTCTCACGCAATTg   <  2:50780/138‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                       
ATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACC‑TATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGG  >  NZ_CP022686/1633717‑1633980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A