Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F35 I0 R1 157 6985.9 8006972 98.3% 7870853 134.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,633,846 T→G 30.5% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,633,8460TG30.5% 177.2 / 51.6 166N253T (AAC→ACC) yqeBselenium‑dependent molybdenum cofactor biosynthesis protein YqeB
Reads supporting (aligned to +/- strand):  ref base T (33/80);  new base G (48/5);  total (81/85)
Fisher's exact test for biased strand distribution p-value = 2.03e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

AACATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGC  >  NZ_CP022686/1633714‑1633974
                                                                                                                                    |                                                                                                                                   
aaCATTATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCCCTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTAttt                                                                                                                                <  5:338949/138‑1 (MQ=255)
 aCATGATCACTTTAAAACACTCATGATATAGACGTAGCTCTAAACCACTGGCGATATCCCCCGCACCTCGAATCACCACCAGCTGGTGTACTGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTAtttt                                                                                                                               <  3:652991/138‑1 (MQ=255)
   aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTc                                                                                                                             <  6:281222/138‑1 (MQ=255)
   aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAc                                                                                                                           <  8:484201/140‑1 (MQ=255)
        cACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGGTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTg                                                                                                                        <  1:355609/138‑1 (MQ=255)
        cACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑Ta                                                                                                                     <  7:470684/140‑1 (MQ=255)
        cACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAACCtt                                                                                                                        >  2:792609/1‑137 (MQ=255)
          cTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCCTCTGGTGTCCGGAAGTAGGAGCCTGCTGCATCATCAACCCACCCGGCGCGTTATTTTAGACCTG‑TAat                                                                                                                   >  8:127452/1‑140 (MQ=255)
                     gacagATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGcc                                                                                                          <  1:182996/134‑1 (MQ=255)
                     gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAAGCCTCCCGTCGCGTTATTTTTCACCTG‑TAATATTTCTGcc                                                                                                          <  4:461360/138‑1 (MQ=255)
                     gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGcc                                                                                                          <  3:612463/138‑1 (MQ=255)
                     gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAg                                                                                                        <  7:35160/140‑1 (MQ=255)
                     gCATGATACAGACGGAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCCGCTGGTGTCCGGACGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCACCTG‑TAATATTTCTGcc                                                                                                          <  6:491103/138‑1 (MQ=255)
                       aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTACACTG‑GAATAATTCTGCCac                                                                                                        >  3:873108/1‑137 (MQ=255)
                       aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAAACAT‑TAATATTTATGcccg                                                                                                        >  6:382017/1‑136 (MQ=255)
                       aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGATTTTTAGCCTG‑TAATATTTATGCCac                                                                                                        >  2:625042/1‑137 (MQ=255)
                       aTGATACAGACGTAGAGCTACACCACTGGCGATATCCCCCGCACCGCGATTCAGCACCAGCTGGAGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAGCTG‑TAATATTTCTGCCAg                                                                                                        <  1:14826/138‑1 (MQ=255)
                       aTGAGACAGACGTAGCGCTACACCCCTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCa                                                                                                      <  8:384542/140‑1 (MQ=255)
                             ccgACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑Taa                                                                                               <  8:914989/138‑1 (MQ=255)
                                  gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATgg                                                                                            <  4:871704/138‑1 (MQ=255)
                                  gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATgg                                                                                            <  6:471846/138‑1 (MQ=255)
                                  gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCg                                                                                          <  7:57061/140‑1 (MQ=255)
                                  gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAAAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATgg                                                                                            <  4:124099/138‑1 (MQ=255)
                                  gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAAGCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATgg                                                                                            <  1:121928/138‑1 (MQ=255)
                                   tAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCCACCCACCCGGCGCGTTATTTTTCACCTG‑GAATATTTATTCCCGCACGC‑TAATGGCGa                                                                                         >  8:761947/1‑140 (MQ=255)
                                    aGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCg                                                                                          <  5:691623/138‑1 (MQ=255)
                                    aGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCg                                                                                          <  5:146352/138‑1 (MQ=255)
                                    aGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCg                                                                                          <  3:430294/138‑1 (MQ=255)
                                     gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTAACCGG‑TTATATTTATTCCAGCACGC‑GAATTGCGAta                                                                                       >  7:184225/1‑139 (MQ=255)
                                     gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAg                                                                                                        <  8:545255/124‑1 (MQ=255)
                                     gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCGGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGATTTTTCACCGG‑GAATATTTGTGGCAGCCCCC‑GAATGTCgc                                                                                         >  4:191044/1‑137 (MQ=255)
                                       gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTATTTTTAACCTG‑GAATATTTAAGCCAGCACCC‑AAATTGCGaac                                                                                       >  6:812641/1‑136 (MQ=255)
                                       gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTT‑TAATATTTTTGCCAGCACCC‑TAATGGCGAta                                                                                       >  2:566152/1‑137 (MQ=255)
                                       gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTGTTTTTTAACCAG‑TAATTATTGTGGCAGCCCGC‑GAATgggggtc                                                                                       >  4:75545/1‑133 (MQ=255)
                                       gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTGATTTTTCACCAG‑TTATATTTATGCCAGCACGC‑TAATGGCGAtc                                                                                       >  2:333035/1‑138 (MQ=255)
                                       gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTCACCGG‑TAATATTTCTGCCAGCACGC‑TAATTGCGaga                                                                                       >  5:299188/1‑136 (MQ=255)
                                       gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAACCGG‑TAATATTTATGCCAGCCCGC‑GAATGGctgtg                                                                                       >  2:182996/1‑134 (MQ=255)
                                       gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAACCGG‑TAAAATTTATGCCCGCACGC‑AAATTGCgcta                                                                                       >  6:514928/1‑135 (MQ=255)
                                       gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACAGG‑TAATATTTATGCCAGCACGC‑TAATGGCGAtc                                                                                       >  2:854113/1‑138 (MQ=255)
                                       gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACAACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTTTTTTTTCCCTC‑GAATAATTAGGACCGCACGC‑GTATGTCGAtg                                                                                       >  5:229027/1‑137 (MQ=255)
                                       gcTACACCACTGGCGATATCCCCCGCACAGCGAATCACCACCAGCTGGTGTCCGGAAGGATGATAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCACCTG‑GAATTTTTATTCCAGCCCCC‑TAAAggggctc                                                                                       >  4:849686/1‑133 (MQ=255)
                                          acacCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAc                                                                                                                           <  8:721130/101‑1 (MQ=255)
                                          acacCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCAc                                                                                                                           >  7:721130/1‑101 (MQ=255)
                                          acacCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACCTG‑TAATATTTCTGCCAGCACGC‑GAATGGCGATCTcc                                                                                    >  2:273243/1‑138 (MQ=255)
                                             ccacTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCCTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTgc                                                                                 <  1:837277/138‑1 (MQ=255)
                                              cacTGGCGATATACCCCTCACCGCGAATCACCTCCATCTGGTGTACTGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCAACTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTgcg                                                                                <  6:829342/138‑1 (MQ=255)
                                                cTGGCGATATCCCCCGCACCGCGAATCCCCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATGAACCCACCCGGCGCGGTTTTTTTAAACTG‑TAATTTTACTTCCAGGCCGC‑GAATGGCGCTCTCCTgcggcgg                                                                            >  7:442066/1‑139 (MQ=255)
                                                cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGCGGTTATTTTAACCAG‑TTATATTTTTGGCGGCCCCC‑GAATGGCGAACTCCTgccgcct                                                                            >  7:412024/1‑138 (MQ=255)
                                                  ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTAAGATG‑TTTTTTTTATTGCAGCACGA‑GAATGGCGGTCTACTGCGGGGt                                                                            >  2:960792/1‑138 (MQ=255)
                                                  ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTAAACTG‑GAATATTTGAGCCACCACCC‑TAATGGCGATCACCAgcggcgg                                                                            >  4:235397/1‑137 (MQ=255)
                                                  ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTTACCAGTTTAAATTATTCCCAG‑ACGC‑TAAATGGGATCTCCTgccgggg                                                                            >  1:383440/1‑133 (MQ=255)
                                                  ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTGACCTG‑TAATATTTATGCCAGCACGC‑GAATGGCGATCTCCTGCGGCGt                                                                            >  4:993581/1‑138 (MQ=255)
                                                  ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGACCTG‑TAATTATTATGCCCCCACGC‑ATATGGCGGTATGCTtcggcgg                                                                            >  6:855155/1‑137 (MQ=255)
                                                  ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGTTTTTTGACCTG‑TAATATTTATGGCCGCCCCC‑TAATTGGGATCTCCTGCGGCGt                                                                            >  4:529428/1‑138 (MQ=255)
                                                   gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGtt                                                                           <  1:906402/138‑1 (MQ=255)
                                                   gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGtt                                                                           <  4:519647/138‑1 (MQ=255)
                                                     gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTc                                                                         <  3:290477/138‑1 (MQ=255)
                                                     gATATCCCCAGCACCGCGAATCACCACCAGATGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCTACTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTGCTGCGGCGTTTc                                                                         <  5:473405/138‑1 (MQ=255)
                                                          cccccGCACCTCGAATCACCACCAGCTGGTGTCCGGTAGGATGAGAAGGCTTCATCATCAACCCACCCGTCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCAc                                                                    <  4:602686/138‑1 (MQ=255)
                                                          cccccGCACCGCGAATCTCCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACTC‑TAATGGCGATCTCCTGCGGCGTTTCCGCAc                                                                    <  4:701763/138‑1 (MQ=255)
                                                          cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTATTTTGAACGG‑TAAAAATTAAGCCCGCACGC‑TAAATGCGCTGTACTGCCGCGTTTACCCAc                                                                    >  3:404501/1‑138 (MQ=255)
                                                          cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACCTG‑TAATATTTATGCCAGCACGC‑TAATGGCGCTATCCGGCGGCGGTTCCGCAc                                                                    >  6:88230/1‑138 (MQ=255)
                                                          cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                  <  8:211222/140‑1 (MQ=255)
                                                          cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACCTG‑TTATTTTTATGGCAGCACGC‑TAATTGGGATATACTGCGGCGGTTACGCAc                                                                    >  3:465537/1‑138 (MQ=255)
                                                          cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCGTCATCATCAACCCACCCGGCGAGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCAc                                                                    <  2:429487/138‑1 (MQ=255)
                                                          cccccGCACCGCGAATCACCACCAGCTGGAGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAAACTG‑TAATATTTATGCCAGCCCGC‑TAATGGCGATCTACTGCGGCGTTTCCGccc                                                                    >  2:442156/1‑136 (MQ=255)
                                                                cACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTAtgt                                                              <  1:273243/138‑1 (MQ=255)
                                                                cACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTAtgt                                                              <  1:698029/138‑1 (MQ=255)
                                                                  ccGCGAATCCCCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTAtgtt‑g                                                           <  4:289735/138‑1 (MQ=255)
                                                                   cgcgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAACCAG‑TATTATTTATTCAAGCACCCATAAT‑GCGATAAACTGCGGCGGTTCAGCACCTAtttt‑gtta                                                        >  7:150310/1‑138 (MQ=255)
                                                                     cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACCTG‑TTATATTTATGCCAGCACGC‑GAATGGCGATCGACTGCGGCGTTTCCGCCCCAATGTT‑GTaa                                                        >  3:711191/1‑138 (MQ=255)
                                                                     cgAATCACCACCAGCGGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAGACCCCCCGGCGCGTTATTTTTCGCCGG‑TAATAATTCTGCCCGCAAGC‑TAAAGGCCATAGCCTGCGGCGATTCCGCCCCTATGGT‑GGAAcc                                                      >  8:599211/1‑140 (MQ=255)
                                                                       aaTCACCACCAGCTGGTGTCTGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTAACCTG‑TAATATTTCTGACACCACGC‑GAATGGCGATCACCATCCGCGGATCCACCCCTATGTT‑TTaaac                                                      >  6:158030/1‑136 (MQ=255)
                                                                       aaTCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGTTTTTTGACCTG‑TATTATTTCTTGCCGCCCGC‑TAATGTGGGTCTCCTGCGGGCGTTGAGCACCGATGTT‑GTAAcc                                                      >  3:417721/1‑138 (MQ=255)
                                                                           accaccAGCTGGTTTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCGGCGGCGTTTCCGCACCTATGTT‑GTAACCAACg                                                  <  4:196336/138‑1 (MQ=255)
                                                                           accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACg                                                  <  6:272412/138‑1 (MQ=255)
                                                                           accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACg                                                  <  1:525898/138‑1 (MQ=255)
                                                                                  gCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCAt                                           <  5:493584/138‑1 (MQ=255)
                                                                                   cTGGTGTCCGGAACGATGAGAAGGCGTCATCATCAACCCACGCGGCGCGTTATTTTTCTAGTG‑TAATATTTCTGCCAGTACGC‑TAATGGAGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCATg                                          <  6:222266/138‑1 (MQ=255)
                                                                                    tGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCATGt                                         <  3:5372/138‑1 (MQ=255)
                                                                                     ggTGTCCGGAAGGATGAGTAGTCTTCATCATCAACCCACACGGCTCTTTATTTTTGTCGTG‑TATTATTTGTGCCTGCACGC‑TAATGTCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GGAACCAACGGGCGCATGTa                                        <  6:623624/138‑1 (MQ=255)
                                                                                     ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGAGCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCATGTa                                        <  2:540596/138‑1 (MQ=255)
                                                                                         tCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCATGTAAACggg                                  <  7:853020/140‑1 (MQ=255)
                                                                                         tCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCGG‑TAATAATTCTGCCAGCACGC‑GAATGGCGCTCTCCTGCGGCGTTTCCCCACCAATGTT‑GTTACCAACGGGCGCAAGTAAACg                                    >  6:893932/1‑138 (MQ=255)
                                                                                         tCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACCTG‑TAATATTTATGGCAGCACGC‑TAATGGGCATCTGCTGCGGCGTTTACGCCCCAATGTT‑TTAACCAACGGGCGCATGTAAACg                                    >  4:367056/1‑138 (MQ=255)
                                                                                            ggAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTTACCAG‑TAATATTTGTTACCACCCGC‑TAATTGTGATCTCCAGCGGGGTTTACGCCCCGATGTTGGTAACC‑ACCGGCGGATGTTAAAgggg                                 >  3:31405/1‑137 (MQ=255)
                                                                                             gAAGGATGAGAAGGCTTCATCATCAACCCACCAGGCGCGTTGTTTTTCACCTG‑TAATATTTGTGGCAGCAAGC‑TAATTGCGATAGCCTTCTGCGTTTCAGGACCTATTTT‑GTAAACAAAGGGGGCATTTTAACtggggat                              >  8:151086/1‑136 (MQ=255)
                                                                                              aaGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCATGTAAACGGGCaa                               <  1:438413/138‑1 (MQ=255)
                                                                                              aaGGATGAGAAGGCTTCATCATCAACCCACCCGGAGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCATGTAAACGGGCaa                               <  1:433505/138‑1 (MQ=255)
                                                                                               aGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTGACCAG‑TAATATTTATGCCAGCCCGC‑TAATTGCGATCTCCTGCGGCGTTTACGCACCAATTTT‑TTAAACAACGGGCGCATGTAAAAGGGCaaa                              >  4:653464/1‑137 (MQ=255)
                                                                                                   tGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAAACTG‑TAATATTTAAGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGGTTCCGCACCTATTTT‑GTAACCAACGGGCGCATGTAAACGGGCAATAtttta                        >  8:753342/1‑139 (MQ=255)
                                                                                                   tGAGAAGGCTTCATCATCAACCCACCCGGCGCGTGTTTTTTCACCAT‑TAATAATTATGGCAGCAAGC‑GAAATGCGATATCCATCGGCCTTTCCCCACCTATGAT‑GTAACCAACGGTCGCAAGTAAACGGGGAAAATGt                          >  4:902548/1‑138 (MQ=255)
                                                                                                         ggCTGCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCa                    <  1:601398/138‑1 (MQ=255)
                                                                                                          gCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACCAG‑TAATATTTATGCCCGCACCC‑TAATGGCGCTCTCATGCGGCCTTTACGCACCTATGTT‑GTAACCAACCGGGGCCTGTAAACGGGCAATATGTTATTCac                   >  4:992504/1‑137 (MQ=255)
                                                                                                          gCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACCAG‑TTATATTTATGCCAGCACGC‑GAAATGCGATCTCCTGCGGCGTTTACGCACCTATGTT‑GTAACCAACGGGCGCAGGTAAACGGGCAATATGTTATTCAg                   >  4:694628/1‑138 (MQ=255)
                                                                                                             tcatcatcaACCCACCCGGCGCGGTATTTTTAACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGGTTCCGCACCTATTTT‑TTAACCAACGGGCGCATGTAAACGGGCAATATTTTATTAAGccc                >  6:974546/1‑137 (MQ=255)
                                                                                                              catcatcaACCCACCCGGCGCGTTTATTTTGAACAT‑TAATAATTCTGCACGCCCGC‑AAATTGCGATATACTGCCGCGGTTACGCACCGATTTT‑GTAACCCACGGGGGGATGTAAACGGGCGATATGTTGTTGAGcccc               >  4:316806/1‑136 (MQ=255)
                                                                                                              catcatcaACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGc             <  8:519250/140‑1 (MQ=255)
                                                                                                              catcatcaACCCACCCGGCGCGTTATTTTTAAACTG‑TAATAATTATGCCAGCCCCA‑TAAATGCGATCTACAGCGGCGTTTAAGCACCGATGTT‑GTAAACAACGGGCGCATTTTAACCGGGAATATTTTCTTAACCCAc               >  2:55396/1‑138 (MQ=255)
                                                                                                                           ccgggCGCGTTATTTTTCACCTG‑TAATATTTAAGCCAGCACGA‑TAATGGCGATATCCTGCGGCGTTTCCTCACCTATGTT‑GTAAGCAACGGGCGCATGTAAACGGGCAATATTTTCTTAAGACACTACTTTATAACGc  <  2:172072/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
AACATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACCTG‑TAATATTTCTGCCAGCACGC‑TAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTT‑GTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGC  >  NZ_CP022686/1633714‑1633974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A