Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F35 I0 R1 195 10216.4 8585726 98.3% 8439768 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,633,846 T→G 30.3% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,633,8460TG30.3% 221.1 / 45.5 183N253T (AAC→ACC) yqeBselenium‑dependent molybdenum cofactor biosynthesis protein YqeB
Reads supporting (aligned to +/- strand):  ref base T (32/92);  new base G (55/3);  total (88/95)
Fisher's exact test for biased strand distribution p-value = 7.04e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

CACTTCCAACATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGC  >  NZ_CP022686/1633707‑1633981
                                                                                                                                            |                                                                                                                                       
cACTTCCAACATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑Gt                                                                                                                                         <  4:70059/140‑1 (MQ=255)
        aCATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTAtttt                                                                                                                                   <  6:388930/138‑1 (MQ=255)
          aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCAc                                                                                                                               <  3:166943/140‑1 (MQ=255)
          aTGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCAc                                                                                                                               <  7:523637/140‑1 (MQ=255)
          aTGATCACTTTAAAACACGCATGATACAGACGTAGCTCTTCACCACTGGCTATATCCCACGCACCGCGAATCTCCACCAGCTGGTGTCCGGAATGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCaa                                                                                                                               <  2:957193/140‑2 (MQ=255)
             ctCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCACCGCACCGCGAAACACAACCAGATGGTGTCAGTATGGATGAGAAGTCTTCATCAGCAACCCACCCCGCGC‑CCTattattacactg                                                                                                                            >  2:127565/2‑134 (MQ=255)
             aTCACTTTAAAACCCGCATGATACAGACGTAGCTCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTATTTTacactt                                                                                                                             >  7:853113/1‑138 (MQ=255)
             aTCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGC‑GTTATTTTTAACCTg                                                                                                                            >  3:680082/1‑140 (MQ=255)
             aTCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTAAACTg                                                                                                                            >  3:868437/1‑140 (MQ=255)
               cACTTTAAATCCCGCATGATACAGACGTAGCTCTACACCAATTGCGATATCCCCCGCACCGGGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCTTCAACGCACCCGGCGC‑GTTATTTTTCACCTGTa                                                                                                                          <  1:698767/140‑1 (MQ=255)
               cACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTa                                                                                                                          <  2:838049/140‑1 (MQ=255)
                      aaaCCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGC‑GGTATTTTTAACCTGTAAtttt                                                                                                                     >  6:216106/1‑138 (MQ=255)
                      aaaCCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCGTCATCATCAACCCACCCGGCGC‑GGTATTTTTGACCGGTTATAtt                                                                                                                     >  6:111479/1‑138 (MQ=255)
                            gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGCATCACAACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTACCCTGTAATATTTGTGCCac                                                                                                             >  4:98895/1‑139 (MQ=255)
                            gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGATGGATGAGAAGGCTTCATCTTCAACCCACCCGGCGC‑GTTATTTTTCAACTGTAATATTTATggccc                                                                                                             >  8:239445/1‑136 (MQ=255)
                            gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAGCCCACCCGGCGC‑GGTTTTTTTGACCGGTTATTTTTATGCCAg                                                                                                             >  8:568578/1‑140 (MQ=255)
                            gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGcc                                                                                                               <  5:534992/138‑1 (MQ=255)
                            gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGcc                                                                                                               <  5:744894/138‑1 (MQ=255)
                            gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAg                                                                                                             <  4:486441/140‑1 (MQ=255)
                            gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAg                                                                                                             <  1:775123/140‑1 (MQ=255)
                            gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTTTTTTTGACCCTTAATATTTATTCCac                                                                                                             >  1:81940/1‑139 (MQ=255)
                            gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTTTTTTTAACCAGTAATATTTTTTCCac                                                                                                             >  1:561203/1‑139 (MQ=255)
                            gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGACGC‑GTTATTTTTCACCTGTAATATTTCTGCCAg                                                                                                             <  4:244736/140‑1 (MQ=255)
                            ccaagATACAGACGTAGCGCGACACCCCTGCCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCTTCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGcc                                                                                                               <  6:144561/134‑1 (MQ=255)
                              aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCa                                                                                                           <  1:859329/140‑1 (MQ=255)
                                    cAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCt                                                                                                       <  6:774447/138‑1 (MQ=255)
                                    cAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTaa                                                                                                     <  8:464178/140‑1 (MQ=255)
                                       aCGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTTACCAGTAATATTTATGACAACAAGCaaaatg                                                                                                  >  2:793271/1‑137 (MQ=255)
                                         gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCg                                                                                                <  1:56861/140‑1 (MQ=255)
                                         gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCg                                                                                                <  7:276437/140‑1 (MQ=255)
                                         gTAGAGCTACACCACTTGCGATATCCCCCGCACAGCTAATCACCACCAGCTGGTGTCCGGAATGATGAGAAGTCTTCATCATCAAACCACCCGGCGC‑GTTATTTTTCACCTGTATTATTTCTGCCAGCACGCTAATgg                                                                                                  <  5:593717/138‑1 (MQ=255)
                                         caggcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCg                                                                                                <  3:749888/137‑1 (MQ=255)
                                          tAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTGAGTTTTCACCGGTTATAATTATGCCAGCACCCTAAAGGCgg                                                                                               >  1:144065/1‑139 (MQ=255)
                                          tAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTAACCTGTAATATTTGTGGCAGCACGCGAATGGCgg                                                                                               >  3:405870/1‑139 (MQ=255)
                                           aGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGATAAGGCTTCATCATCAAACCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCg                                                                                                <  6:46430/138‑1 (MQ=255)
                                           aGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGAt                                                                                              <  1:962074/140‑1 (MQ=255)
                                            gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTTTTTTTCAACGGTAATTATTCTTCCCGCCCGCGAATGGGGaaa                                                                                             >  3:806637/1‑138 (MQ=255)
                                            gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTAttttt                                                                                                                                  <  1:626981/103‑1 (MQ=255)
                                            gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTGACCTGTAATATTTATTCCAGCAAGCTAATGGGGAta                                                                                             >  3:773909/1‑139 (MQ=255)
                                            gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTCACCTGTAATATTTCTGCCAGCACGCGAATGGCGCtc                                                                                             >  2:982862/1‑140 (MQ=255)
                                            gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTCACAGGTAATATTTATGCCAGCACGCTAATGGCGAtc                                                                                             >  4:640595/1‑140 (MQ=255)
                                            gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTACCCTGTTATATTTATGGCAGCACGCGAATGGCGAtc                                                                                             >  4:675576/1‑140 (MQ=255)
                                            gcgcTACACCACTGGCGATATCCCCAGCACCGCGAATCACCACCAGCTGGTGTCCTGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCCCCTGTAATTTTTTTGGCAGCACGCGTATGGCGAtg                                                                                             >  4:886666/1‑139 (MQ=255)
                                            gcgcTACACAACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACACGGCGC‑GGTTTTTTTCACAGGGTATATTTATGCCAGCCCGCGAAttgagctc                                                                                             >  8:365664/1‑133 (MQ=255)
                                             cgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGC‑GGGGTTTTTGGCCTGGAATTTTTTTGGCCGCCCGCGAATGGCGGtca                                                                                            >  7:227364/1‑139 (MQ=255)
                                             cgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTTTTTTTAACCTGGAATATTTATGGCCGCCCGCTAATTGCgctaa                                                                                            >  7:705749/1‑136 (MQ=255)
                                              gcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCAGTAATATTTCTGCCCGCACGCTAATGGCGAta                                                                                             >  5:845695/1‑137 (MQ=255)
                                                 acacCACTGGCGCTATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTg                                                                                        <  8:277728/140‑1 (MQ=255)
                                                  caccacTGGCGATATCCCCCGCACCGCGAATCTCCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTGACCGCTAAAATTTCTGCCCGCCCGAGAAAGGGGCTCTCCAgc                                                                                       >  3:211022/1‑140 (MQ=255)
                                                    ccacTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCAGTAATATTTATGCCAGCACGCGAATGGCGATCTACTgc                                                                                       >  5:664763/1‑138 (MQ=255)
                                                     cacTGGCGATATACCACGCACCGCGAATCCCCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTgcg                                                                                      <  6:580479/138‑1 (MQ=255)
                                                         ggCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTAACCTGTAATATTTATGCCAGCACGCTAATGGCGCTCTCCAGCGGCGt                                                                                  >  6:640920/1‑138 (MQ=255)
                                                            gagatCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCATCATCATCAACCCCCCCGGCGC‑GTGATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCg                                                                             <  1:471230/137‑1 (MQ=255)
                                                            gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCg                                                                             <  1:1035685/140‑1 (MQ=255)
                                                            gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCg                                                                             <  2:380710/140‑1 (MQ=255)
                                                            gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGGTTCCg                                                                             <  4:320327/140‑1 (MQ=255)
                                                            gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATATCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTc                                                                               <  5:481333/138‑1 (MQ=255)
                                                               atCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGC‑GGTATTTTTAAACAGTTATATTTGTTCCCCCCACCGAATGGCGATCTCCTGAGGCGattaccccc                                                                          >  7:471021/1‑134 (MQ=255)
                                                               atCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTTTTTTTGACCGGTTATTATTATTCCAGCACGCGAATGGGGGTCTCCAGCGGCGGTTCCGCAc                                                                          >  1:759830/1‑140 (MQ=255)
                                                                 cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGGTTTTTTGACCTGTTATATTTCTGCCAGCCCGCTAATGGCGAGCTACAGCGGCGTGTACGccc                                                                          >  5:813411/1‑136 (MQ=255)
                                                                 cccccGCACCGCGAATCACCACCAGCTGGGGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                        <  2:441844/140‑1 (MQ=255)
                                                                 cccccGCACCGAGAATCACCACCAGCGGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCt                                                                        <  7:970910/140‑1 (MQ=255)
                                                                  ccccGCACCGCGAATCACCACCAGCTTGTGTCCTGTAGGATGAGAAGGCTGCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACGTa                                                                       <  1:286492/140‑1 (MQ=255)
                                                                     cGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACACACCCGGCGC‑GGTTTTTTTACCCTGTAATATTTATGCCACCACGCTAAAGGCGATCTCCTCCGGCGTTACCGCACCTAtgt                                                                    >  2:910838/1‑140 (MQ=255)
                                                                      gCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTCAACTGTAATATTTATGCCAGCCCGCTAATGGCGATCGCCTGCGGCCTTTCCGCCCCTAtt                                                                     >  6:65865/1‑137 (MQ=255)
                                                                            cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTTTTTTTCACCGGTAATATTTCTGCCAGCACGCTATTGGCGATCTCCTGCGGCGTTTCCGCACCGAAGTTGTAAcc                                                             >  2:688852/1‑140 (MQ=255)
                                                                            cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTGACCTGTAATATTTCAGCCAGCACGCGAATGGCGATAGCCGGCGGCGTTTCCGCACCGATGTTGGaaac                                                             >  8:637280/1‑138 (MQ=255)
                                                                            cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTAAACGGTTATAATTATTCCAGCCCCCTAAATGGGATATCCAGCGGCGGTTACGGCCCGAtgttgttaac                                                             >  1:34752/1‑136 (MQ=255)
                                                                            cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTGACCTGTAATATTTATTCCCGCACGCTAATGGCGATCTCCTGCGGCGGTTACGCCCCTATTTTGTAAcc                                                             >  8:399457/1‑140 (MQ=255)
                                                                                  cccaccaGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGA‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACg                                                         <  5:846186/137‑1 (MQ=255)
                                                                                  accaccAGCTGGTGTCCGGAAGGATGAGCAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACggg                                                       <  3:48932/140‑1 (MQ=255)
                                                                                  accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACggg                                                       <  1:102297/140‑1 (MQ=255)
                                                                                  accaccAGCTGGGGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCACCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACggg                                                       <  2:320857/140‑1 (MQ=255)
                                                                                          cTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTa                                               <  3:719061/140‑1 (MQ=255)
                                                                                          cTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTCACCAGTAATAATTCTGCCAGCACCCAAATGGGGATATCCTGCGGGGTTTCCGCCCCTATTTTTGAACCAACGGGCGCATGTa                                               >  4:768616/1‑140 (MQ=255)
                                                                                            ggTGTGCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                             <  7:984214/140‑1 (MQ=255)
                                                                                            ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                             <  2:709178/140‑1 (MQ=255)
                                                                                            ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                             <  1:582488/140‑1 (MQ=255)
                                                                                            ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                             <  3:954057/140‑1 (MQ=255)
                                                                                            ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTa                                               <  5:346218/138‑1 (MQ=255)
                                                                                             gtgtCCGGAAGGATGAGAAGGCTTCATCATCCACCCACCCCGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAAc                                            <  2:359890/140‑1 (MQ=255)
                                                                                              tgtCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTAACCTGTAATATTTATTCCAGCACGCTCATGGCGAGATCCAGCGGCGTTTCCGCCCCATAGTTGTAACCAACCGGGGCTTGTTAAcc                                           >  8:686025/1‑139 (MQ=255)
                                                                                                tCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACggg                                         <  8:759096/140‑1 (MQ=255)
                                                                                                  cGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCa                                       <  4:732168/140‑1 (MQ=255)
                                                                                                  cGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTGTTCACCTGTAATATTTCTGCCAGCACTCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGTCGCATGTAAACGGGCa                                       <  4:472716/140‑1 (MQ=255)
                                                                                                     aaGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAata                                    <  2:1098744/140‑1 (MQ=255)
                                                                                                     aaGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAata                                    <  2:629929/140‑1 (MQ=255)
                                                                                                      aGGATGCGAAGGCTTCATCATCAACCCACCCGGAGC‑GTTATTTTTCACCTGTAATATTTCTGCCATCACGCTAATGGCGATCTCCTGCGGCGTTGCCGCACCTATGTTGTAACCAACGGGCGCATTTAAACGGGCAat                                     <  6:933518/138‑1 (MQ=255)
                                                                                                            agaAGGCTTCATCATCAACCCACCCGGCGC‑GGTATTTTTCAACTGTAATATTTATTCCAGCACGCTAAAGTAGATCTCCTGAGGCGTTTACGCACATATTTTGTAACAAACGGGCGCATGTAAAATGTCAATAtttta                               >  6:458906/1‑137 (MQ=255)
                                                                                                            agaAGGCTGCATCATCAACCCACCCTGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGttc                               <  6:944783/138‑1 (MQ=255)
                                                                                                                ggCATCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATAATTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCa                           <  6:542812/138‑1 (MQ=255)
                                                                                                                ggCATCATCATCAACCCACCCGGCGA‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGc                         <  8:119014/140‑1 (MQ=255)
                                                                                                                  cTTCATCATCAACCCACCCGGCGC‑TGTTTTTTTGACATTTAAAATTTATGCCCGCACCCTAAATGCGCTATACTGCCGCGATTACGCACCAATGTTTTAACCAACGGGCGCATGTAAACGGGCAATATGttattaccccc                       >  2:400592/1‑134 (MQ=255)
                                                                                                                   ttCATCATCAACCCACCCGGCGC‑GGTATTTTTGACCAGTAATATTTATGCCAGCACGCGAATGGCGCTCTCCTGCGGCGGTTCCGCCCCTATGTTGTAACCCACCGGCGCCAGTAAACGGGCAATATGTTCTTCAGcccc                      >  2:1073495/1‑138 (MQ=255)
                                                                                                                       tcatcaACCCACCCGGCGC‑GGTTTTTTTGAACTGTTATATTTATGCCAGCACGCGAATGGCGATATCCAGCGGCGGTTACCCACCAATTTTGTAACCAACGGGCGGAGGTTAACGGGCAATATGTTATTAAGCCACGc                    >  6:925830/1‑138 (MQ=255)
                                                                                                                           caACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTtct              <  2:831162/140‑1 (MQ=255)
                                                                                                                             acccacccGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCa            <  3:11568/140‑1 (MQ=255)
                                                                                                                                  cccGGCGC‑GGTATTTTTGACCTGTAATATTTCTGACAGCACGCTAATTGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCACCGGGCGCCTGTAAACGGGCAATATGTTCTTCAGCCACGCCATTCTCACGCaa       >  2:776683/1‑140 (MQ=255)
                                                                                                                                  cccGGCGC‑GGTATTTTTAACCTGTAATATTTAATCCAGCACGCGAAAGGCGAACGCCTGCGGCGTTTACGCACCTATGTTGTAAACAACGGGCGCATGTAAACGGGCAAAATTTTCTTCAGCCACGCCATTCTCACGcca       >  2:933824/1‑138 (MQ=255)
                                                                                                                                   ccGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAAt      <  3:94978/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                       
CACTTCCAACATGATCACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGC‑GTTATTTTTCACCTGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGGCGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGC  >  NZ_CP022686/1633707‑1633981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A