Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F13 I0 R1 150 9496.0 7621532 98.4% 7499587 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,110,457 T→G 11.9% K12N (AAA→AAC)  rplM ← 50S ribosomal protein L13

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,110,4570TG11.9% 367.5 / 11.4 217K12N (AAA→AAC) rplM50S ribosomal protein L13
Reads supporting (aligned to +/- strand):  ref base T (79/107);  new base G (29/2);  total (108/109)
Fisher's exact test for biased strand distribution p-value = 3.20e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

CGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAG  >  NZ_CP022686/2110318‑2110595
                                                                                                                                           |                                                                                                                                           
    cAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTa                                                                                                                                         <  7:258377/140‑1 (MQ=255)
    cAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTa                                                                                                                                         <  4:184223/140‑1 (MQ=255)
    cAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGGTTTACCGGTCGCGTCTACAACATACCAGTCGCGTTTTa                                                                                                                                         <  4:239099/140‑1 (MQ=255)
      gAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACg                                                                                                                                       <  8:206195/140‑1 (MQ=255)
         cGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGtt                                                                                                                                    <  8:820163/140‑1 (MQ=255)
            tgatgTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCt                                                                                                                                 >  1:209322/1‑140 (MQ=255)
            tgatgTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACGGTTTCt                                                                                                                                 >  4:651536/1‑140 (MQ=255)
            tgatgTAATCACCGGTATCTACGTGCGGAGGGTATTCCGCGTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTGTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCt                                                                                                                                 <  7:812052/140‑1 (MQ=255)
                 tAATTACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCTCGTCAACAACATACCAGGAGGGTTTTACGGtttaggtttt                                                                                                                            >  5:657856/1‑133 (MQ=255)
                 tAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGttt                                                                                                                            <  4:801229/140‑1 (MQ=255)
                   aTCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAg                                                                                                                          >  5:755222/1‑140 (MQ=255)
                   aTCACCGGTATCTACGTGCGGAGTGTATTCCGCTGTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAg                                                                                                                          <  5:50726/140‑1 (MQ=255)
                      aCCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGCGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTg                                                                                                                       >  1:589104/1‑140 (MQ=255)
                        cGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATAACAGTCGCGTTTTAAGGTTTCTGGTTTAGCTGTa                                                                                                                     >  5:508098/1‑140 (MQ=255)
                        cGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTAAACAACATACCAGTCGCGTTTTACGGTTTATGGTTTAGCTGTa                                                                                                                     >  6:510047/1‑140 (MQ=255)
                                  gTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTGTTACGGTTTCTGGTTTAGCTGTAAAAGgtttga                                                                                                           >  6:890396/1‑138 (MQ=255)
                                     cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTa                                                                                                       <  6:256875/140‑1 (MQ=255)
                                     cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTa                                                                                                       <  7:172586/140‑1 (MQ=255)
                                     cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTa                                                                                                       <  8:342266/140‑1 (MQ=255)
                                     cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTa                                                                                                       <  3:772941/140‑1 (MQ=255)
                                     cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTa                                                                                                       <  3:646021/140‑1 (MQ=255)
                                     cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACGGTTTATGGTTTTGCTGTAAAAGTTTT‑CATTa                                                                                                       >  2:690988/1‑140 (MQ=255)
                                     cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACGGTGTATGGTTTAGCTGTAAAAGTTTT‑CATTa                                                                                                       >  3:145926/1‑140 (MQ=255)
                                     cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTAAGGTTTCTGGTTTAGCTGTTAAATGTTT‑AATTa                                                                                                       >  1:46482/1‑140 (MQ=255)
                                     cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGACGCATTTTTCCGTTTCTGGTTTAGCGGGAAAAATTTT‑CAttt                                                                                                       >  3:878750/1‑139 (MQ=255)
                                     cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGGCAACACCATACCAGTCGCGGTTTTAGGTGTCTGGGTTAGATGTTAAATTTTT‑CAttt                                                                                                       >  8:490309/1‑139 (MQ=255)
                                     cGGAGTGTACTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTCCCGGTCGCGGCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTa                                                                                                       >  8:61798/1‑140 (MQ=255)
                                         gtgtATTCCGCTTTGTGCTTACCGCACAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACCACATACAAGTCGCGGTTTACGGGGTATGGGGTAGCTGTTAAAGTTTT‑CAGTATAAg                                                                                                   >  7:563692/1‑140 (MQ=255)
                                            tATTCCGCTTTGTGCTTACCGCGCAGGCGACTAGCCAGTTCAGTAGCCAGACGGCCCAGAGGTTTACCGGTCGCGTCAGCAACATCCCAGTCGCGTTTTAACGTTTCGGGTTGAGCCGGTAAATTTTT‑AATTAAAAGCtt                                                                                                >  8:763727/1‑140 (MQ=255)
                                            tATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTTCGGTTTATGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCtt                                                                                                >  2:596913/1‑140 (MQ=255)
                                            tATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCtt                                                                                                <  2:150066/140‑1 (MQ=255)
                                              ttCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTAc                                                                                              <  3:426393/140‑1 (MQ=255)
                                               tCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTAcc                                                                                             <  5:597908/140‑1 (MQ=255)
                                                  gCTTTGTGCTTACCGCGCAGGCGACGAGCAAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTGTGGGTTAGCTGTAAAAGTTTT‑AATTAAAAGCTTAcccct                                                                                          >  2:628762/1‑138 (MQ=255)
                                                    tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCaata                                                                                        >  4:959458/1‑140 (MQ=255)
                                                     ttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCaataa                                                                                       <  2:435302/140‑1 (MQ=255)
                                                      tgtgCTTACCGCGCAGGCGACGAGCCAGTTCTGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCaataaa                                                                                      <  1:640500/140‑1 (MQ=255)
                                                      tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCTGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTAT‑CATTAAAAGCTTACCCaataaa                                                                                      <  4:536283/140‑1 (MQ=255)
                                                      tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCaataaa                                                                                      >  4:84417/1‑140 (MQ=255)
                                                      tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACAAGTCGCGTTTTTCGGTTTATTCGTTAGCTGTAAAAGTTTT‑CATTAAAATCTTACCCaataaa                                                                                      >  6:711603/1‑140 (MQ=255)
                                                      tgtgCTTACCGCGCAGGCGACGAGCCAGGTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAAGAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCaataaa                                                                                      <  4:264624/140‑1 (MQ=255)
                                                        tgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGGTTGTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTAACCaaaaaata                                                                                    >  5:769720/1‑140 (MQ=255)
                                                         gCTTACCGCGCAGGCGACGAGCCAGTTCAGTCGCCAGACGGCACAGAGTTTTACCGGTCGCGTCAAAAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTACAAGTTTT‑CATTAAAAGCTTACCCAATAAATAg                                                                                   <  5:496058/140‑1 (MQ=255)
                                                         gCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAg                                                                                   <  1:297742/140‑1 (MQ=255)
                                                           ttACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAATTTTT‑CATTAAAAGCTTACCCAATAAATAGtt                                                                                 <  4:213092/140‑1 (MQ=255)
                                                            tACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATGAATAGTTa                                                                                <  5:472439/140‑1 (MQ=255)
                                                             aCCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTTCGGTTTCTGGTTTATCTGTAAAAGTTTT‑CATTAAAAGCTTACACAATAAATAGTTaa                                                                               >  1:544509/1‑139 (MQ=255)
                                                             aCCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTAAGGTTTCTGGTGTAGCTGTAAAACTGTT‑CATTAAAATCTGACCCAATAAATAGTTac                                                                               >  8:679863/1‑140 (MQ=255)
                                                             aCCGCGCAGGCGACGAGCCAGTTCAGTAGACAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTTCGGGTTCAGGTTTATCAGGAAAAGTTTTAAATTAAACGGTTA‑CCAATAAATAGttta                                                                               >  8:350277/1‑138 (MQ=255)
                                                              ccGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTaca                                                                              <  4:488393/140‑1 (MQ=255)
                                                              ccGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTaca                                                                              <  2:52126/140‑1 (MQ=255)
                                                                gcgcAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACg                                                                            <  6:487997/140‑1 (MQ=255)
                                                                gcgcAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACg                                                                            <  1:679241/140‑1 (MQ=255)
                                                                gcgcAGGCGACGAGCCAGGTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACg                                                                            <  6:142856/140‑1 (MQ=255)
                                                                  gcAGGCGACGCGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTGGTACCGGTTCTGGGTTAGCTGTAAAAGTTTT‑AATTAAAAGCTTACCCAATAAATAGGTACACGtt                                                                          >  2:455903/1‑140 (MQ=255)
                                                                       cgacgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTGTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGa                                                                     <  2:254119/140‑1 (MQ=255)
                                                                        gacgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGaa                                                                    <  2:404961/140‑1 (MQ=255)
                                                                               cAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCaa                                                             <  3:476146/140‑1 (MQ=255)
                                                                                 gTTCAGTAGCCAGACGGACCAGAGTTGTACCGGTCGCGTCAACAACATACCAGGCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAAc                                                           <  6:162637/140‑1 (MQ=255)
                                                                                  ttCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTAAGGGTTCTGGTTTAGCTGTAAAAGTTTT‑AATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGACCACCCacacc                                                          >  6:742819/1‑139 (MQ=255)
                                                                                       tAGCCAGACGGCCCAGAGGTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACGGTTTCTGGGTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATACTTAAACGTTTGTGAACACCCAAACGTCTTc                                                     <  1:619071/140‑1 (MQ=255)
                                                                                            aGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGGTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAttg                                                >  4:303656/1‑140 (MQ=255)
                                                                                            aGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCAGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACACAATAAATAGTTTCACGTTGGTGAACACCCAAACGGCTACAAtat                                                >  4:56571/1‑138 (MQ=255)
                                                                                              aCGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTATT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAttgtt                                              <  1:392373/140‑1 (MQ=255)
                                                                                                 gCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAg                                           <  6:380000/140‑1 (MQ=255)
                                                                                                   ccAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGt                                         <  6:46141/140‑1 (MQ=255)
                                                                                                    cAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGGTTCTGGTTTATCTGTTAAAGTTTT‑CATTAAAAGCGTACCCAATAAAAAGTTACACGTTTGTGTACACCCAAACGTCGTCAATTTTTGAGGtt                                        >  8:713682/1‑140 (MQ=255)
                                                                                                      gagTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATCGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTca                                      <  2:125208/140‑1 (MQ=255)
                                                                                                            tACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCGGGTTTTGCTGTAAAATTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGAc                                >  5:586300/1‑140 (MQ=255)
                                                                                                             aCCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACa                               <  7:648899/140‑1 (MQ=255)
                                                                                                                 gTCGCGTCAACAACATACCAGTCGCGTTTTACGGGTTCTGGTTTAGGTGTAAAAGTTTT‑CATTAAAAGCTTAACCAATAAATAGTTTCACGTTGGTGAACAGACAAACGTCTGCAATTGTTTAGGTTCACACGAAAAAGt                           >  8:538159/1‑140 (MQ=255)
                                                                                                                   cgcgTCAACAACATACCAGTCGCGTTTTACGGTTTGTGGTTTAGCTGTAAAAGTTTT‑CATTAAAACCATACCCAATAAATATTTACACGTTGGGGAACACCCAAACGTCTTCAATTGTTTAGGTTAACACGCCAAAGTcc                         >  3:107583/1‑140 (MQ=255)
                                                                                                                   cgcgTCAACAACATACCAGTCGCGTTTTACGGTTTATTGTTTAGCTTTAAAATTTTT‑CATTAAAAGCGTACCCAAAAAATAGTTAAACGTTTGTGAACACCCCAACGAATAGAATTGTTGAGGTTCACACGACAAACTcc                         >  6:597213/1‑140 (MQ=255)
                                                                                                                   cgcgTCAACAACATACCAGTCGCGTTTTACGGGTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTAACCAATAAATAGTTAAACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTcc                         >  4:116746/1‑140 (MQ=255)
                                                                                                                   cgcgTCAACAACATACCAGTCGCGGTTTAAGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTAACCAATAAAAATTTAAAAGTTGGTGAACACCCAAACGTCATAAATTGTTGAGGTTCACACGACAAAGTca                         >  7:60120/1‑139 (MQ=255)
                                                                                                                     cgTCAAAATCATACCAGTCGCTGTTTACGGTTTCTGGTTTCGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCgg                       <  7:463289/140‑1 (MQ=255)
                                                                                                                      gTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGc                      <  1:978645/140‑1 (MQ=255)
                                                                                                                      gTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGc                      <  7:30657/140‑1 (MQ=255)
                                                                                                                      gTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGc                      <  4:508074/140‑1 (MQ=255)
                                                                                                                             acaTACCAGTCGCGTTTTCCGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTa               <  1:268838/140‑1 (MQ=255)
                                                                                                                                tACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAccc            >  4:904145/1‑140 (MQ=255)
                                                                                                                                tACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAccc            >  4:868387/1‑140 (MQ=255)
                                                                                                                                tACCAGTCGCGTTTTACGGTTTATGGTTTAGCTGTAAAAGTTTT‑CAATAAAATCTTACCAAATAAATAGTTACACGTTGGTGAAAACCCAAACTTCTTAAATTGTTTAGGTTCACACGACAAAATCCTGCAAACCTAccc            >  6:52981/1‑140 (MQ=255)
                                                                                                                                 aCCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAcccc           <  3:542258/140‑1 (MQ=255)
                                                                                                                                 aCCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAATTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAcccc           <  7:373732/140‑1 (MQ=255)
                                                                                                                                  ccAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCt          <  3:84417/140‑1 (MQ=255)
                                                                                                                                   cAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCgg                       <  8:271915/126‑1 (MQ=255)
                                                                                                                                   cAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCtt         >  2:939372/1‑140 (MQ=255)
                                                                                                                                     gTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATATTTACACGTTGGTGAACACCCAAACGGCTTCAATTGTTGAGGTTCCCCCGACAAAGTCCGGCCAACATACCCCTTCg       >  8:662681/1‑140 (MQ=255)
                                                                                                                                     gTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTAACCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTAACACGACAAAGTCCGGCAAACCTACCCCATcc       >  2:217426/1‑139 (MQ=255)
                                                                                                                                      tCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGa      <  7:70402/140‑1 (MQ=255)
                                                                                                                                      tCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCAAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGa      >  3:386357/1‑140 (MQ=255)
                                                                                                                                       cgcgTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa     <  1:130711/140‑1 (MQ=255)
                                                                                                                                       cgcgTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa     <  8:396143/140‑1 (MQ=255)
                                                                                                                                       cgcgTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa     <  3:394183/140‑1 (MQ=255)
                                                                                                                                       cgcgTTTTAAGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa     <  5:814328/140‑1 (MQ=255)
                                                                                                                                        gcgTTTTACGGGTTCTGGGTTAGCGGTAAAAGTTTT‑CAGTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCACACCTACCCCTGAGAAt    >  6:131098/1‑140 (MQ=255)
                                                                                                                                         cgTTTTACGCTTTATGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTAACCAAAAAAAAGTTAAACGTAGGTGAACCCCCAAACCTCATCAATTTTTGAGCTTCACCCGACAAACTCCGGCCAACCTCCCCCTTCGACTa   >  2:381242/1‑140 (MQ=255)
                                                                                                                                          gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAg  <  5:253661/140‑1 (MQ=255)
                                                                                                                                          gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAg  <  3:275534/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTT‑CATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAG  >  NZ_CP022686/2110318‑2110595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A