Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F36 I0 R1 179 8076.5 8499890 98.3% 8355391 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,110,457 T→G 12.2% K12N (AAA→AAC)  rplM ← 50S ribosomal protein L13

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,110,4570TG12.2% 372.2 / 10.4 201K12N (AAA→AAC) rplM50S ribosomal protein L13
Reads supporting (aligned to +/- strand):  ref base T (75/94);  new base G (27/3);  total (103/98)
Fisher's exact test for biased strand distribution p-value = 2.03e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

GTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGC  >  NZ_CP022686/2110319‑2110596
                                                                                                                                          |                                                                                                                                            
gTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGtt                                                                                                                                             >  4:326194/1‑140 (MQ=255)
   cAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTa                                                                                                                                          <  3:863764/140‑1 (MQ=255)
   cAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGAGTCAACAACATACCAGTCGCGTTTTa                                                                                                                                          <  6:900687/140‑1 (MQ=255)
         gatgatGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTGAACAACATACCAGTCGCGTTTTACGGttt                                                                                                                                    >  5:126174/1‑140 (MQ=255)
         gatgatGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGttt                                                                                                                                    <  4:76724/140‑1 (MQ=255)
         gatgatGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACAGACCAGGCGCGTTTTACGGttt                                                                                                                                    >  2:425094/1‑140 (MQ=255)
         gatgatGTAATCACCGGTAACTACTTGCGGAGTGTATTCAGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTATCAAGACGGCCCAGAGTTTTACCGGTCGCGTCCACAACATACAAGTCGCGTTTTAAGGttt                                                                                                                                    >  6:444014/1‑140 (MQ=255)
         gatgatGTAATCACCGGTAACTACGTGCGGAGTGAATTCCGCTATGTGCTTACCGCGCAGGCGACGAGCCAGATAAGTATCCAGACGGCCCAGAGTTTTACCGGTCACGACAACACCAAACCAGACGAGTTATACggatg                                                                                                                                    >  6:29872/1‑137 (MQ=255)
          atgatgTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTc                                                                                                                                   <  2:527584/140‑1 (MQ=255)
           tgatgTAATCACCGGTATCTACGTGCGGAGCGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGtttat                                                                                                                                  >  4:283807/1‑138 (MQ=255)
             atgGAATCACCGGTATCTAAGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACTAGACAGTGCAGTAGCCAGACGGCCCAGATTTTTACCGGTCGCGACAACAACCTACCAGTCGCGTGTTACGGgttgg                                                                                                                                  >  8:7396/1‑136 (MQ=255)
                tAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAAAATACAAGGAGGGGTGTACGGGGTCTGGttt                                                                                                                             >  3:780903/1‑140 (MQ=255)
                     aCCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTGTGGTTTGGC‑Tg                                                                                                                       >  6:643295/1‑140 (MQ=255)
                     aCCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑Tg                                                                                                                       >  4:1082994/1‑140 (MQ=255)
                     aCCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTGTTAGGGTTTCTGGGTTAGC‑Tg                                                                                                                       >  5:777947/1‑140 (MQ=255)
                         gTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTa                                                                                                                     >  8:897832/1‑138 (MQ=255)
                            tCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACACCATACCAGTCGCGTTTTAGCGTTTCTGGGTTAGG‑AGTAAAAGt                                                                                                                >  5:598129/1‑140 (MQ=255)
                              tACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTTCGGGTTGGGGGTTAGC‑TTTaaaatt                                                                                                                >  8:288002/1‑136 (MQ=255)
                              tACGGGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGttt                                                                                                              <  5:88538/140‑1 (MQ=255)
                                 gTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGCCGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTTTGGTTTGTGGGTTTTC‑GGTATAAGtgttga                                                                                                           >  4:421974/1‑138 (MQ=255)
                                 gTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACAGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCa                                                                                                           >  6:239929/1‑140 (MQ=255)
                                    cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGCGTTTTACCGGTCGCGTCAACAACATACCCGTCGCGTTTTACGGTTTGTGGTTTAGC‑TGTTAAAGTTTTCATTa                                                                                                        >  6:586011/1‑140 (MQ=255)
                                    cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTa                                                                                                        >  4:811715/1‑140 (MQ=255)
                                    cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTa                                                                                                        <  5:475714/140‑1 (MQ=255)
                                    cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTa                                                                                                        <  3:217371/140‑1 (MQ=255)
                                    cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTGTTAGGGTGTGTGGTTTAGC‑TGGAAAAGTTTTAAttt                                                                                                        >  5:931262/1‑139 (MQ=255)
                                    cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTTCGGGTGTTGGTTTTGCGTGT‑AAAGttttatttt                                                                                                        >  5:396296/1‑135 (MQ=255)
                                    cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACGGTTTCTGGTTTATC‑TGTAAAAGTTTTAATTa                                                                                                        >  1:299858/1‑140 (MQ=255)
                                      gAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGGTTAGC‑TGTAAAAGTTTTCAttt                                                                                                        >  8:550388/1‑137 (MQ=255)
                                           tATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCtt                                                                                                 <  1:287323/140‑1 (MQ=255)
                                           tATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCtt                                                                                                 <  3:968065/140‑1 (MQ=255)
                                           tATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCtt                                                                                                 <  1:563365/140‑1 (MQ=255)
                                           tATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCtt                                                                                                 >  2:36036/1‑140 (MQ=255)
                                           tATTCCGCTGTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTGGCGGGTTCTGGTTTAGG‑TGGATAAGTTTTCATTAAAAgg                                                                                                   >  8:1011377/1‑137 (MQ=255)
                                             ttCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGGCGCGTCATCAACATACCCGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCtt                                                                                                 <  8:450581/138‑1 (MQ=255)
                                              tCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTATCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTAcc                                                                                              <  6:667563/140‑1 (MQ=255)
                                              tCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTAcc                                                                                              <  4:514420/140‑1 (MQ=255)
                                                cGCTTTGTGCTTACCGCGCAGGCGTCGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTAcc                                                                                              <  8:322021/138‑1 (MQ=255)
                                                 gCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGGCGCGTGTTTCGGGTTCTGGTTTAGC‑GGGAAAAGATTTGATTAAAAGCTTGcccca                                                                                           >  2:201889/1‑138 (MQ=255)
                                                   tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTTCGGTTTGTGGTTTAGC‑TGTTAAAGTTTTAATTAAAAGCTTTCCCaata                                                                                         >  2:926326/1‑140 (MQ=255)
                                                   tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCaata                                                                                         <  6:150372/140‑1 (MQ=255)
                                                   tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCaata                                                                                         <  2:668607/140‑1 (MQ=255)
                                                   tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGGTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCaa                                                                                           >  8:486205/1‑138 (MQ=255)
                                                   tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGGTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCATACCCaata                                                                                         >  2:1017288/1‑140 (MQ=255)
                                                   tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTAAGGTTTGTGGTTTATC‑TGTAAAAGTTTTAATTAAAATCATAACCaata                                                                                         >  3:10765/1‑140 (MQ=255)
                                                   tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTTCGGTTTGTGGTTTAGC‑TTTAAAAGTTTTAATTAAAATCGTACCCaata                                                                                         >  3:326356/1‑140 (MQ=255)
                                                   tgtgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTAACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCaata                                                                                         <  5:973622/138‑1 (MQ=255)
                                                     tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAAGATACCAGTCGCGGTTTTAGGTTTCTGGGTTAGA‑TGTAAAAGTTTTGATTAAAATCTTACACaataaa                                                                                       >  3:948762/1‑140 (MQ=255)
                                                     tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCaataaa                                                                                       <  4:119349/140‑1 (MQ=255)
                                                     tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACGGTTTCTGGTTTTGC‑TGTAAAATTTTTCATTTAAAGCTTACCCaataaa                                                                                       >  4:393747/1‑140 (MQ=255)
                                                     tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACGGTTTCTGGTGTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACACaata                                                                                         >  7:903315/1‑138 (MQ=255)
                                                       tgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGGCGCGTTTTACGGTTTGTGGGTTAGC‑TGGAAATGTTTTCATTAAAAGCATACACaataaaaa                                                                                     >  1:696504/1‑138 (MQ=255)
                                                            aCCGCGCAGGCGCCGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACCTACCAGGCGCGTTTTACCGGTTCGGGGTGAGC‑TGGAAAAGTTTTCCTTAACACCTTAAGCAAAAAATAGATaa                                                                                >  5:137585/1‑139 (MQ=255)
                                                            aCCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTaa                                                                                >  1:113326/1‑139 (MQ=255)
                                                                      cgacgaGCCAGTGCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTTGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGa                                                                      <  5:676607/140‑1 (MQ=255)
                                                                       gaggaGCCAGTTCAGTAGACAGACGGCGAAGAGTTTTACCGGTCGCGTCAACAACATACCAGTTGCGTTTTACGGGTTCTGGTTTAGC‑TTTAAATGTTTTCTTTATATGCTTACGCATTAAATAGTTACACGTTGGTGaa                                                                     <  1:835165/137‑1 (MQ=255)
                                                                       gacgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGaa                                                                     <  1:892294/140‑1 (MQ=255)
                                                                       gacgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGaa                                                                     <  2:55728/140‑1 (MQ=255)
                                                                       gacgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGaa                                                                     <  6:986796/140‑1 (MQ=255)
                                                                       gacgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGGCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGaa                                                                     <  4:902551/140‑1 (MQ=255)
                                                                               aGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGGTTCTGGGTTAGC‑GGTATAAGTTTTCATTAAAATCGTACCCAATAAATAGTTACAAGTTGGGGAACACCCaaa                                                             >  1:1072357/1‑140 (MQ=255)
                                                                                 ttCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCGGGTTTAGC‑TGTTAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTAAACGTTGGTGAACACCCcaa                                                             >  8:690214/1‑138 (MQ=255)
                                                                                      tAGCCTGACGGCAGAGAGTGTTACCGGTCGCGTCAACAACATACCAGTCGCTTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACACAAACGTCTTc                                                      <  5:528865/140‑1 (MQ=255)
                                                                                      tAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTc                                                      <  1:726082/140‑1 (MQ=255)
                                                                                        gCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCaa                                                    <  4:842821/140‑1 (MQ=255)
                                                                                             ccGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCTCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAttgtt                                               <  3:683167/139‑1 (MQ=255)
                                                                                             aCGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAttgtt                                               <  3:536717/140‑1 (MQ=255)
                                                                                             aCGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAttgta                                               <  3:1082997/140‑2 (MQ=255)
                                                                                              cGGCCCAGAGTTTTAACGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACGCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCATttgttg                                              <  2:770906/140‑1 (MQ=255)
                                                                                              cGGCCCAGAGATTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGATACACGTTGGGGAACACCCAAACGTCTTCAAttgt                                                <  7:461420/138‑1 (MQ=255)
                                                                                                     tagTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TTTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGGTGGTGAACACCCAAACGTCTTCAATTGTTGAGGtt                                         <  7:665721/137‑1 (MQ=255)
                                                                                                      agTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTcac                                      <  1:597634/140‑1 (MQ=255)
                                                                                                           tACCGGTCGCGTCAACAACATACCAGTCGCGGTTTAACGGTTGTGGTTTATG‑GGTAAAAGTTTTGATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACCCCCCAAAGGCGTCCAATGTTGAGGGTCACACGAc                                 >  5:829946/1‑140 (MQ=255)
                                                                                                             ccGGTCGCGTCAACAACATACCAGTCGCGTTTTAAGGTTTATGGTTTTTC‑TGTAAAAGTTTTCATTAAAATAATACCCAAAAAATAGTTAAAATTTGGTTAACACCCAAACGTCTTCAATTTTTGAGGTTAACAAGaa                                 >  7:172237/1‑137 (MQ=255)
                                                                                                                gTCGCGTCAACAACATACCAGTCGCGGTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTTAAAGCTTACCCAATAAATAGTTACAAGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGt                            >  2:80234/1‑140 (MQ=255)
                                                                                                                    ctgcaacaaCATACCAGTCGCGGTTTACGGTTGCTGGTTTATC‑TGTAAAAGTTTTCATTAACAGCTTCCCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCgg                        <  5:725423/137‑1 (MQ=255)
                                                                                                                    cgTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTa                                                                                                <  7:865886/68‑1 (MQ=255)
                                                                                                                     gTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCg                         <  8:373760/138‑1 (MQ=255)
                                                                                                                      tCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATATTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCa                      <  3:465397/140‑1 (MQ=255)
                                                                                                                            acaTACCAGTCGCGTTTTACGGTTTCTGTTTTAGC‑TGTAATAGTTTGCATTAAAAGCTAACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGCGGTGCACACGACAAAGTCCGGCAAACCTa                <  6:373695/140‑1 (MQ=255)
                                                                                                                            acaTACAAGGCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAAcc                  <  7:341357/138‑1 (MQ=255)
                                                                                                                              aTACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAcc              <  1:213921/140‑1 (MQ=255)
                                                                                                                                aCCAGTCTCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAcccc            <  1:622149/140‑1 (MQ=255)
                                                                                                                                 ccAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCt           <  6:471510/140‑1 (MQ=255)
                                                                                                                                  cAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAttg                                                 <  7:51782/101‑1 (MQ=255)
                                                                                                                                    gTCGCGTTTTACGGGTTCTGGTTTAGC‑TGTAAAATTTTTCATTAAAAGCATACCCAATAAATAGTTACACATTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACCCGACAAAGTCCCGCACACCTACCCCTTCg        >  4:203437/1‑140 (MQ=255)
                                                                                                                                     tCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAt                                                   >  4:68316/1‑96 (MQ=255)
                                                                                                                                     tCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGa       >  2:947719/1‑140 (MQ=255)
                                                                                                                                     tCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTAACCAATAAATATTTACACGTTGGTGAAAACCCAAAAGTCTTCAATTTTTTAGGTTAAAACGACAAAGTACGGCAAACCTACCCCTTCGa       >  5:323866/1‑140 (MQ=255)
                                                                                                                                     tCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGGTTTCATTAAAATCTTACCCAATAAAAAGTTACACGTTGGTGAACCCCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAACCCTACCCCATCgc       >  6:788921/1‑139 (MQ=255)
                                                                                                                                     tCGAGTTTTACGGTTTCCGGTATAGA‑TGTAAAAGTTTTAAGTAAAAGCATACCCAAAAAATAGTTACAAGTTGGTGCACACCCAAACGTCTTAAATTTTTGAGGTTCACACGACAAAGTCAGGAAAACCTACCCCttagc       >  2:255671/1‑137 (MQ=255)
                                                                                                                                      ggcgTTTTACGGTTTCTGGTTTAGC‑TGTAAAATTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGGTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCg        <  7:807616/137‑1 (MQ=255)
                                                                                                                                      ggcgTGTTACGGTTTCTGGTTTAGC‑TGTAATAGTTTTCATTAAAAGCTAACCCAATAAATAGTTACACGTTGGGGAACACCCAAACGTCTTAAATTCTTGAGGGTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa      <  5:218699/139‑1 (MQ=255)
                                                                                                                                      cgcgTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa      <  3:657217/140‑1 (MQ=255)
                                                                                                                                      cgcgTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa      <  3:178530/140‑1 (MQ=255)
                                                                                                                                      cgcgTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGGCTTCAATTTTTGAGGTTCCCACGACAAAGTCCGGCAAACCTACCCCTTCg        >  8:864371/1‑138 (MQ=255)
                                                                                                                                       tcgTTTTAACGATTCAGGTTTAGC‑TGTTAAATTTTTCATTAAAAGCTTACCAAAAAAATAGATACACCTTGGTGAACTCCCAAACGAATTCAATTGTTGAGGTTGACAACGCAAAGTACGGCAAACCAAACCCTTcaa       >  8:796836/2‑136 (MQ=255)
                                                                                                                                       gcgTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAAAAAATAGTTACACCTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAAt     >  4:732372/1‑140 (MQ=255)
                                                                                                                                       gcgTTTTACGGGTTCTGGTTTAGC‑TGTAAAAGTTTTAATTAAAATCTTACCCAATAAATAGTTACACGTTGGTGAACACCCACACGTCTTAAATTGTTGAGGTTCACACGAAAAAGTCCTGCAAACCTACCCATTCGa       >  8:630618/1‑138 (MQ=255)
                                                                                                                                       gcgTTTTAAGGTTTCTGGTTGAGC‑TGTAAAAATTTTCATTAAAAGATTAACAAATAAATAGTTAACCATTGGGGAAAACCCAAACGTCGTCAATTTTTGAGGGTCACACGACAAAGTCCGGCAAACCGACCCCGTCGa       >  8:156539/1‑138 (MQ=255)
                                                                                                                                          |                                                                                                                                            
GTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGC‑TGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGC  >  NZ_CP022686/2110319‑2110596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A