Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F35 I0 R1 195 10216.4 8585726 98.3% 8439768 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,110,457 T→G 15.6% K12N (AAA→AAC)  rplM ← 50S ribosomal protein L13

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,110,4570TG15.6% 350.4 / 13.6 211K12N (AAA→AAC) rplM50S ribosomal protein L13
Reads supporting (aligned to +/- strand):  ref base T (71/99);  new base G (34/4);  total (107/104)
Fisher's exact test for biased strand distribution p-value = 4.85e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGC  >  NZ_CP022686/2110320‑2110596
                                                                                                                                          |                                                                                                                                           
ttCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑Gttt                                                                                                                                           >  4:539799/1‑140 (MQ=255)
  cAGAACGTTGATGTAATCACCGGTATCTTCGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTa                                                                                                                                         <  4:163167/140‑1 (MQ=255)
  cAGAACGATGATGTAATCACCGGTATCTACGTGGGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGTCGAGCCAGGTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTa                                                                                                                                         <  8:369981/140‑1 (MQ=255)
  cAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTa                                                                                                                                         <  7:10584/140‑1 (MQ=255)
       cGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAAACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACgg                                                                                                                                      <  6:352380/138‑1 (MQ=255)
            atgTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTACGGTTTCt                                                                                                                                 >  6:285548/1‑138 (MQ=255)
                 aTCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGttt                                                                                                                            >  6:484174/1‑138 (MQ=255)
                 aTCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGttt                                                                                                                            <  5:786051/138‑1 (MQ=255)
                    aCCGGTATCTACTTGCGGAGTGTATTCCGCTTTGTGATTACCGCGCAGCCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTCCCGGTCGAGTCAACAACAGAGCTGTCGC‑GGTTTAGGGTTGCTTGGTTAGCTg                                                                                                                       >  4:82699/1‑140 (MQ=255)
                      cGGTATCTCCGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGCCGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATGCCCGTCGC‑GTTTTACGGGTGGTGGttttggtg                                                                                                                       >  6:24698/1‑133 (MQ=255)
                      cGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTACGGTTTGTTGTTTAGCTGtt                                                                                                                     >  3:1053132/1‑139 (MQ=255)
                      cGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGGCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGtt                                                                                                                     >  1:1069535/1‑139 (MQ=255)
                        gTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTaaa                                                                                                                   >  2:526253/1‑140 (MQ=255)
                           tCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGt                                                                                                                >  7:249329/1‑140 (MQ=255)
                                gTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGCCGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTGTACGGTTTCTGGTTTAGCGGTAAAAGTTTTCa                                                                                                           >  4:64331/1‑140 (MQ=255)
                                gTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTTCGGGTTCTGGTTTAGGTGGAAAAGttttga                                                                                                           >  2:1050793/1‑138 (MQ=255)
                                   cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGCGCCAGTTCAGTAGCCAGACGGCCCAGAGTTATACCGGTCGCGTCAACAACATACCAGTCGC‑GATTTAAGCTTTCTGGGTTAGCGGTAAAATttttgaata                                                                                                        >  1:207233/1‑135 (MQ=255)
                                   cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTa                                                                                                        <  7:959997/140‑1 (MQ=255)
                                   cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTAACGGTTCTGGATTATGAGTTAAAGTTTTAAttt                                                                                                        >  1:346656/1‑139 (MQ=255)
                                   cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTACGGGTTAAGGTTTATAGGTAAAAGTTTTAATTa                                                                                                        >  1:215918/1‑140 (MQ=255)
                                   cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGGCGC‑GGTTTACGGGTTCTGGGTTAGGGGTAAAATTTTTAAttt                                                                                                        >  7:92827/1‑139 (MQ=255)
                                   cGGAGTGTATTCCGCTTTGTGCATACCGCGCACGAGACCAGCCAGAACCGAATCCAGACGGCCCAGAGTTTTACCGGTCGCGGCAACACCATACCAGTCCC‑GTTTTACCGATTCTGGTTGAGCTCTAAAAGattaaattt                                                                                                        >  4:903362/1‑134 (MQ=255)
                                     gAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGCCGGCCCAGAGTTTTACCGGTCGCGTCAACAACCTACCAGTCGC‑CTTTTACGGGTTCGGGTTTAGCTGTAAAAGTTTTCATTa                                                                                                        >  6:812688/1‑138 (MQ=255)
                                     gAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACAAACCAGTAGC‑GATTTAAGGTTGATGGTTTTGCATTAAAAAGTTTCagtt                                                                                                        >  5:1029622/1‑135 (MQ=255)
                                        tttagtCCGCGTAGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTGTACCGGCCGCGTCAACAACATACCAGTCGC‑GTGTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGc                                                                                                   <  4:86651/135‑1 (MQ=255)
                                          tATTCCGCTTTGTGCTTACCGCGCAGGCGACTAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCtt                                                                                                 <  3:841368/140‑1 (MQ=255)
                                           aTTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTa                                                                                                <  4:708690/140‑1 (MQ=255)
                                              ccGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGGTTCTGGTTTAGCTGGAAAAGTTTTCATTAAAAGCGTAc                                                                                               >  6:756754/1‑138 (MQ=255)
                                               cGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTTGCGG‑TTGTGGTTTAGCTGTAAAAGTTTTAATTAAAATCTTACCCa                                                                                            >  3:268463/1‑140 (MQ=255)
                                                gCTTTGTGCTTACCGCGCAGGCGCCGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTATGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTAACCaa                                                                                           >  4:579309/1‑140 (MQ=255)
                                                gCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGGCGC‑GGTTTTCGGTTTGTGGTTTAGCTGTAAAAGTTTTGATTAAAAGCGTTCCCaa                                                                                           >  8:297879/1‑140 (MQ=255)
                                                gCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACAGGTCGCGTCAACAACATACCAGGCGC‑GTTTTAAGGTTTCTGGTTTAGCGGTTAAATATTTCATTTAAAGCTTACCCaa                                                                                           >  7:740708/1‑140 (MQ=255)
                                                 cTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTTAGGTTTATTGTTTTTCTGTAAAAATTTTAATTAAAAGCTTACCCaaa                                                                                          >  3:176245/1‑139 (MQ=255)
                                                  tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaata                                                                                         <  8:195114/140‑1 (MQ=255)
                                                  tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaata                                                                                         <  1:1010194/140‑1 (MQ=255)
                                                  tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTAAGGTTTCTGGGTTTGCTGTATAAGTTTTGATTTAAATCTTACCCaata                                                                                         >  1:562802/1‑140 (MQ=255)
                                                  tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGACGC‑GGTTTACGGTTTCTGGTTTATAGGTAAAAGTTTTAATTAAAAGCTTACACaata                                                                                         >  1:572964/1‑140 (MQ=255)
                                                  ttggtgCTTACCGCGCCGGCGACGAGCCAGTGCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGGCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaata                                                                                         <  3:252273/137‑1 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTCGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTAAGGTTTCTGGTTTAGCGGTAAAAGTTTTCATTAAAAGCTTACCCcataaa                                                                                       >  2:338159/1‑140 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTTCGGTTTGAGGTTTTGGTGGAAAAATTTTAATTAAAATCTTACCCaaaa                                                                                         >  5:970307/1‑136 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTTCGGTTTCTGGTTTAGGTGTAAAAGTTTTCATTAAAAGCTTCCCAaataaa                                                                                       >  2:293531/1‑140 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaataaa                                                                                       <  4:39356/140‑1 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGGAAAAGTTTTCATTAAAAGCTTACCCaataaa                                                                                       >  4:826256/1‑140 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTGTTATGGTTTATGGTTTAGGTGGTAAATTTTTGATTTAAAGCTTAACCaaaaaa                                                                                       >  3:887517/1‑140 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTGTTACGGTTTCTGGGTTAGCTGGAAAAGTTTTCATTAAAAGCTTACCCaataaa                                                                                       >  2:233731/1‑140 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTTCGGTGTCTGGTTTAGCTGTAAAAGTTTTGATTTAAAGGTTACCCaata                                                                                         >  5:630100/1‑138 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTAAGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaaaaaa                                                                                       >  3:102670/1‑140 (MQ=255)
                                                           aCCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCACCAACATACCAGGCGC‑GGTTTACGGTTTAGGGGTTAGCTGGAAAAGGTTTGATTAAAAGCTTACCCAATAAAAAGTTaa                                                                                >  3:234761/1‑139 (MQ=255)
                                                           aCCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGGCAACAACATACCAGTCGC‑GGTTTTCGGGTTGTGGGTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTaa                                                                                >  3:691271/1‑139 (MQ=255)
                                                             cgcgCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGGTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAAAACTTaa                                                                                >  6:546513/1‑137 (MQ=255)
                                                                     ggacgaGCCAGTTCAGTAGCCAGAAGGCCCAGAGTTTTAGCGGTCGCGTCAACAACTTACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGa                                                                      <  2:17364/139‑1 (MQ=255)
                                                                      gacgaGCGAGTTCAGTTGCCAGACGGCCCAGAGTTTTACAGGTCGCGTCAAAAACATCCCAGTCGC‑GTTATACGGTTTCTGGTTTAGCTGTATTAGCTTTCATTAAAAGCTTACCCAATAAATAGTTACAAGTTGGTGaa                                                                     <  4:260370/140‑1 (MQ=255)
                                                                      gacgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTATAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGaa                                                                     <  1:275202/140‑1 (MQ=255)
                                                                      gacgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGGAAAAGTTTTCATTAAAAGCTTACCCACTAAATAGTTACACGTTGGTGaa                                                                     <  1:665723/140‑1 (MQ=255)
                                                                      gaagaGCCAGTTCAGTAGCCAGACGGCCCAGAGTGTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTTGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTg                                                                       <  5:780216/135‑1 (MQ=255)
                                                                       acgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAac                                                                    <  1:780868/140‑1 (MQ=255)
                                                                             cAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACAccc                                                                <  5:376897/138‑1 (MQ=255)
                                                                             cAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCaa                                                              <  2:567235/140‑1 (MQ=255)
                                                                               gTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGATACACGTTGGTGAACACCCAAAc                                                            <  4:333848/140‑1 (MQ=255)
                                                                                     tAGCCAGACGTCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTc                                                      <  2:58495/140‑1 (MQ=255)
                                                                                     tAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATTGTTACACGTTGGTGAACACCCAAACGTCTTc                                                      <  8:1017563/140‑1 (MQ=255)
                                                                                     tAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTATACCGGTTCAGGTTTAGCTGTAAAAGTTTTCATTTAAAGCTTAACCAATAAATAATTACACGTTGGTGAACACCCAAACGTCtta                                                      >  8:321172/1‑139 (MQ=255)
                                                                                     tAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAAAAACATACCAGTCGC‑GGTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCtta                                                      >  8:890166/1‑139 (MQ=255)
                                                                                       gCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCaa                                                    <  8:249329/140‑1 (MQ=255)
                                                                                       gCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTTCGGTTTCTGGGTTAGCTGGAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACTTAGGTGAACCCCCAAACGTCtac                                                      >  6:987001/1‑136 (MQ=255)
                                                                                       gCCAGACGGCCAATAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAATCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCaa                                                    <  8:569710/140‑1 (MQ=255)
                                                                                            aCGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GGTTTACGGGTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTAAACGTTGGTGAACACCCAAACGTCTTCAAttgtt                                               >  8:719330/1‑140 (MQ=255)
                                                                                                    tagTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGtt                                         <  6:931826/137‑1 (MQ=255)
                                                                                                    gagTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTTGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTca                                       <  3:417770/140‑1 (MQ=255)
                                                                                                    gagTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTca                                       <  7:192636/140‑1 (MQ=255)
                                                                                                    gagTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTca                                       <  1:427607/140‑1 (MQ=255)
                                                                                                    gagTTTTACCGGTCGCGGCAACAACATACCAGTCGC‑GGTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTca                                       <  8:790837/140‑1 (MQ=255)
                                                                                                     tgTTTTACCGGTCGCGTCAACTACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTc                                        <  5:503060/137‑1 (MQ=255)
                                                                                                     agTTTTACCGGTCGCGTCAACAACTTACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAATGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTcac                                      <  3:738894/140‑1 (MQ=255)
                                                                                                          tACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTATGGTTTAGCTGTTAAATTTTTAATTTAAATCTTTACCAATAAATAGTTACACGTTGGTGAACACCCCAACGTCTTCAATTTTTGAGGTTCACCCgca                                 >  1:786756/1‑138 (MQ=255)
                                                                                                           aCCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACa                                <  2:1105856/140‑1 (MQ=255)
                                                                                                               gTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGt                            <  2:585555/140‑1 (MQ=255)
                                                                                                               gTCGCGTCAACAACATACCAGTCGC‑GTTTTAAGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTAAACGTTGGTGAACAACCCAACGTCTTAAATTGTTTAGGTTCACACGACaaatt                            >  4:1048051/1‑138 (MQ=255)
                                                                                                                   ggTCAATAACATACCAGTCGA‑GTTTTATGGTTTCTGGTTTAGCTTTAAAAGTTTTCATTAAAAGCTTACCCAATAAATATTTTCACGTTGGTGAACACCTAAACGTGTTCAATTGTTGAGGTTCACACGTCAAAGTCCgg                        <  8:720505/139‑1 (MQ=255)
                                                                                                                   cgTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCgg                        <  1:935740/140‑1 (MQ=255)
                                                                                                                    gTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGTTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCAAATAAATAGTTACACGTTGGTGAAAACAAAAACGTCTTCAATTTTTGAGGTTCACACGACAAAGTCCg                         <  6:1029622/138‑1 (MQ=255)
                                                                                                                           acaTACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTa                <  4:133625/140‑1 (MQ=255)
                                                                                                                            caTACCAGTCGC‑GGTTTACGGGTTCTGGTTTAGCTGTAAAATTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGGCGTCAATTGTTGAGGTTCACACGCCAAAGTCCGGGAAACCTAc               >  2:896966/1‑140 (MQ=255)
                                                                                                                             aTACCAGTCTC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTa                <  6:857034/138‑1 (MQ=255)
                                                                                                                             aTACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTc                                                      <  2:682298/100‑1 (MQ=255)
                                                                                                                             aTACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAcc              <  2:125654/140‑1 (MQ=255)
                                                                                                                                ccAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTa                                                                                 >  6:199098/1‑70 (MQ=255)
                                                                                                                                ccAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGGTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAACTCCGGCAAACCTAccc             >  5:667600/1‑138 (MQ=255)
                                                                                                                                   gTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAATCTAAACAAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTTAGGTTAACACGACAAAGTCCGGCAAACCTACCCCTTCg        >  7:433390/1‑140 (MQ=255)
                                                                                                                                   gTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACACAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGATGTTCACACGACAAAGTCCGGCCAACCTACCCCTTCg        >  4:1029285/1‑140 (MQ=255)
                                                                                                                                    tCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGa       >  4:779798/1‑140 (MQ=255)
                                                                                                                                     cgc‑gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCg        <  5:849062/138‑1 (MQ=255)
                                                                                                                                     cgc‑gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa      <  7:862116/140‑1 (MQ=255)
                                                                                                                                     cgc‑gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa      <  1:959600/140‑1 (MQ=255)
                                                                                                                                     cgc‑gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa      <  8:424330/140‑1 (MQ=255)
                                                                                                                                     cgc‑gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa      <  1:526253/140‑1 (MQ=255)
                                                                                                                                     cgc‑gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa      <  3:806136/140‑1 (MQ=255)
                                                                                                                                     cgc‑gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGGGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa      <  7:307489/140‑1 (MQ=255)
                                                                                                                                     cgc‑gGTTTACGGTTTCTGGTTTAGCTGGACAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCg        <  5:218705/138‑1 (MQ=255)
                                                                                                                                          ttttACCGTTTATGGTTTATCAGTAAAAGTTTTAAATAAAAGCTTACCCAAAAAAAAGTTACACGTTTGTGAACACCCAAACGTCTTCAATTGTTGAGGGTCACACGACAAAGTACGGCAAACCTACCCCTACCAATatc  >  2:978930/1‑138 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGC‑GTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGC  >  NZ_CP022686/2110320‑2110596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A