Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F2 I0 R1 252 7301.5 9636800 98.5% 9492248 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,925,289 A→C 10.1% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,925,2890AC10.1% 361.9 / 5.0 176N156T (AAC→ACC) prpC2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (114/38);  new base C (6/17);  total (120/56)
Fisher's exact test for biased strand distribution p-value = 8.42e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

ATGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTT  >  NZ_CP022686/3925150‑3925428
                                                                                                                                           |                                                                                                                                            
  gcgcACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACaa                                                                                                                                              >  2:541622/1‑138 (MQ=255)
   cgcACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑c                                                                                                                                            >  1:1108696/1‑138 (MQ=255)
     cACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGAGTCTTCTCGACTGTTAGCACTACAGCCACAA‑Cggag                                                                                                                                        >  6:52167/1‑138 (MQ=255)
      aCCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACCCCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCgc                                                                                                                                       >  6:733350/1‑139 (MQ=255)
       ccGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGCTTGTTCTCTAGTGGGATCGCTCACGCCACAA‑CGGCg                                                                                                                                        >  1:540427/1‑138 (MQ=255)
       ccGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCg                                                                                                                                        <  1:729304/138‑1 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCCCGCTGCCAGAAAAAGAGGGGCCCACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAAc                                                                                                                                     >  6:761250/1‑140 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGa                                                                                                                                       >  2:740376/1‑138 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGAGTCTTCTCTACTGGTATCACTACAGCCCCAA‑CGGCgc                                                                                                                                       >  1:480132/1‑137 (MQ=255)
          gCGTTTCCGCGCTCGGCTGCACGCTGCCAGAATAAGCGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGATCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGc                                                                                                                                   >  7:680734/1‑140 (MQ=255)
           cGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACCCCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCGGGCGTCACTTAGTTCGATTCTTGTCTACTGGTATCACTACAGCCCCAA‑CGTCGAACGCa                                                                                                                                  >  4:1165753/1‑140 (MQ=255)
             tttCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATc                                                                                                                                <  3:784718/140‑1 (MQ=255)
                     cTCGGCTTCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCg                                                                                                                          <  2:438278/138‑1 (MQ=255)
                     cTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACGCAGTTTCTGGTGCGCGGGATATTGCCGCCAAACTGCTGGCGTCAGTTAGTTCGATTCTTCGCTACGGGTATCACTACAGCCACAC‑AGGCGAACGCATCCAGCCGGa                                                                                                                        >  5:485291/1‑140 (MQ=255)
                     cTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATTTTGCCGACATACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAC‑CGGCGAACGCTTCCAGCCggg                                                                                                                        >  8:1106958/1‑139 (MQ=255)
                     cTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGa                                                                                                                        <  3:912652/140‑1 (MQ=255)
                       cGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGaaa                                                                                                                      <  5:835091/140‑1 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATgacgac                                                                                                           >  4:912784/1‑140 (MQ=255)
                                    ccAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGCTTCTTCTCTACTGGTATCACTACAGCCACCA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGActc                                                                                                         >  4:1224422/1‑140 (MQ=255)
                                    ccAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAC‑CGGCGAACGCATCCAGCCGGAAACTGATgacgac                                                                                                           >  2:905327/1‑138 (MQ=255)
                                    ccAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATgacgac                                                                                                           >  2:383505/1‑138 (MQ=255)
                                    ccAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGActc                                                                                                         >  4:970036/1‑140 (MQ=255)
                                          aaaGAGGGGCACCCCGTTTCTGGGGCGCGGGATATTGCCGACAAACTGCGGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACACCCACAA‑CGGCGAACGCACCCCGCCGTAAACTGATGACGACTCTATcgg                                                                                                   <  8:32871/140‑1 (MQ=255)
                                              agGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGTCTCTATCGGCGGt                                                                                               <  8:889911/140‑1 (MQ=255)
                                              agGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGt                                                                                               >  7:869579/1‑140 (MQ=255)
                                                gggCACACCGTTTCTGGTGCGCGGGTTATTGCCGAAAAACTTCTGGCGTCACTTTTTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCTAACGCAGCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCa                                                                                             <  4:770440/140‑1 (MQ=255)
                                                gggCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCCCTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCa                                                                                             >  3:1022352/1‑140 (MQ=255)
                                                gggCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGCACGCATCCATCCGGCAACTGATGACGACTCTATCGGCGGt                                                                                               >  1:973728/1‑138 (MQ=255)
                                                  gCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTAAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGATCGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACt                                                                                           >  4:109915/1‑140 (MQ=255)
                                                    acacCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACt                                                                                           >  2:602219/1‑138 (MQ=255)
                                                    acacCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCAACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACt                                                                                           >  1:690577/1‑138 (MQ=255)
                                                      acCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCt                                                                                       >  3:586916/1‑140 (MQ=255)
                                                          tttCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCAt                                                                                   >  4:197559/1‑140 (MQ=255)
                                                          tttCGGGGGCGCGGTATTTTGCCGCCAACCTGCGGGCGTCACTTAGTGCTATGCTTCTCTCCTGGTACCACTCCCGCCACAC‑CGCCGAACCCACCCCCCCGGAAACTTAGGACGACTCTATCGGCGGTCACTTCCTGCAt                                                                                   <  8:518300/140‑1 (MQ=255)
                                                           ttCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAG‑CACAA‑CGGCGAACGCATCCAGCCGGACACTGATGACGACTCTATCGGCGGTCACTTCCTGCATct                                                                                 >  8:64536/1‑140 (MQ=255)
                                                            tCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCAt                                                                                   >  2:360634/1‑138 (MQ=255)
                                                                gTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATctgctg                                                                             >  4:252681/1‑140 (MQ=255)
                                                                 tGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCtgctgc                                                                            >  7:397142/1‑140 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCCCTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATATGCTGCa                                                                           >  6:20784/1‑140 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATAACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCAATCGGCGGTCACTTACTGCATCTGCTGCa                                                                           >  3:99359/1‑140 (MQ=255)
                                                                     cgGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACgg                                                                        >  4:706207/1‑140 (MQ=255)
                                                                     cgGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACgg                                                                        >  8:746824/1‑140 (MQ=255)
                                                                             tGCCGACAAACTGCTGGCGTCACTTAGTACGAAACTTCTCTACTGGTATAACTACAGCCACAA‑CGCCAAACTCATCCAGACGGAAACTCAAGACAACTCAAACCTCCGTAAATAACAGCATCAGCAGCACGGCAAAAAGc                                                                >  6:108762/1‑140 (MQ=255)
                                                                             ccccGACAACCTGCTGGCGCAACTTCTGCCGATTCTTCTCACCTGGTAGCACACCAGCCACAC‑CGCCGAACCCACCCACCCGCAAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGc                                                                <  7:1063375/138‑1 (MQ=255)
                                                                                 gACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCg                                                              >  2:211732/1‑138 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTCCAGCCACAC‑CGGCGCACGCATCCAGCCGGCAACTGAAGCCTCCTCTATCGGCGGTCACTTCCTGCAGCTGCTGCACGGCGAAAAGCCGTCg                                                           >  8:469629/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGCCGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  7:249160/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGCCTCTATCGGCGGGCAGTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  6:292097/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  6:1158706/1‑140 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  2:2603/1‑138 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  2:493490/1‑138 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGcc                                                         >  8:112579/1‑139 (MQ=255)
                                                                                       cgggtgGCGTCACTTAGTTCTATTATGCTGTACTGGTATCACTCCAGCCACAC‑CGGCTAACGCATCCAGCGGGAAACGGAGCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAg                                                      <  5:884720/136‑1 (MQ=255)
                                                                                              cGTCAATTAGTTCTTTTCTGATCTCATGTTAACAATACACCCACAC‑CGGCTAACGCATCCAGTCGTAAACTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTggg                                                 <  1:1008083/138‑1 (MQ=255)
                                                                                               gTCACTTTGTTCGTTTCTTCTCTCCGGGTTTCACTAACCCCACAA‑CGGCTAACCCATCCCGCCGGAAAATCATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGAAAAGCTGGGaaa                                              <  6:810134/140‑1 (MQ=255)
                                                                                                  tcTTAGTTAGATTCTTATCTAGTGGAATCACTACAGCCACAA‑GGGCGAACTCATCCACCGGTAAACTTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGaaaa                                             <  1:406726/137‑1 (MQ=255)
                                                                                                  aCTTAGTTGGTTTCTGCTCTACTGG‑AACACCAACCCCCCAACCGGCGAACGCATCCCGCGGTAAACTTACCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAACGCCGTCGCAAAGCTGGGAAAAgg                                           <  7:918242/140‑1 (MQ=255)
                                                                                                  aCTTAGTTCTCTTGTTCTCTACTTGTAGCCCTACACCCACAA‑CGGCGTCCGCAGCGAGCCGCAAACTGATGACGCCGCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGACAAgg                                           <  4:1056162/140‑1 (MQ=255)
                                                                                                  aCTTAGTTCGATTCTTCTCTACTGGTATCCCTACAGCCACAC‑CGGCTACCGCATCCAGCCGCAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGaaaa                                             <  2:23306/138‑1 (MQ=255)
                                                                                                  aCTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           >  4:804097/1‑140 (MQ=255)
                                                                                                  aCTTAGTTCGATTATTCTCTCCTGGTATCACTACAGCCACAC‑CGGCGAACGAATCCAGCCGTAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGaaaa                                             <  2:850908/138‑1 (MQ=255)
                                                                                                   attAGTTCGATTCTTCTCTACTGGTATCACAACCGCCCCAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGc                                          <  3:189720/139‑1 (MQ=255)
                                                                                                    ttAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAC‑CGGCGAACGCACCCAGCCGGAAACTTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCg                                         <  7:942947/140‑1 (MQ=255)
                                                                                                       tttCGATTCTACTCTACTGTTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATg                                      <  6:647301/139‑1 (MQ=255)
                                                                                                        ttCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGc                                     >  3:907322/1‑140 (MQ=255)
                                                                                                          cGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCGATCGGCCGTCACTTCCTGCATCTGCTGCCCGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGc                                     >  2:75468/1‑138 (MQ=255)
                                                                                                            aTTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 >  7:449872/1‑140 (MQ=255)
                                                                                                            aTTCTTCTCTACTGGTATCACAACAGCCACAA‑CGGCTAACGCATCCAGCCGGAAACTCAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  5:1174319/140‑1 (MQ=255)
                                                                                                             ttcttcTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatata                                >  7:141767/1‑139 (MQ=255)
                                                                                                             ttcttcTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatata                                >  3:229606/1‑139 (MQ=255)
                                                                                                              tcttctCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtct                               >  5:1152048/1‑140 (MQ=255)
                                                                                                              tctcctCTAGTGGTTCCAATACCGCCAAAA‑CGCCGTCCTCTTCCCGCCGGTAACTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtct                               <  8:505218/140‑1 (MQ=255)
                                                                                                               cttctCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAACAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtc                                >  2:66847/1‑138 (MQ=255)
                                                                                                                ttctCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGCCTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCAGTCGCAAAGCTGGGAAAAGGCGATGCATAtct                               >  1:813921/1‑138 (MQ=255)
                                                                                                                 tctcTACTGGTATCACTACACCCCCAA‑CGGCGAACGCAGCCAGCCGTAAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtctc                              <  1:642480/138‑1 (MQ=255)
                                                                                                                 actctACTGGAAGCAATCCAGCCACAC‑CGGCGAACCCATCCACCCGTAAACTGAGCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtctc                              <  1:857907/137‑1 (MQ=255)
                                                                                                                   tctAGTGTTAGCACTACAGCCACAC‑CGGCGAACCAATCCCGCGGGAAACTGTTGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  2:1071077/138‑1 (MQ=255)
                                                                                                                   taaaCTGGTATACCTACAGCCACAC‑CGGCGACCGAACCCCGCCGTAAACTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTg                          <  8:242235/137‑1 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAA‑CGGCGCACGCATCCAGCCGGAAACTGTTGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCGGGTGCTGt                   >  4:142833/1‑140 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGt                   >  3:764542/1‑140 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGt                   >  7:141998/1‑140 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGt                   >  6:307360/1‑140 (MQ=255)
                                                                                                                            aTCACTACATCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAATCTGGTAAAAGGCGATGCATATCTAGCTGGTGCTGt                   >  2:324990/1‑138 (MQ=255)
                                                                                                                            aTCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTAc                 >  6:842464/1‑140 (MQ=255)
                                                                                                                              cACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTAc                 >  2:728127/1‑138 (MQ=255)
                                                                                                                               aCTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACg                >  1:24873/1‑138 (MQ=255)
                                                                                                                               aCTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  5:329740/1‑140 (MQ=255)
                                                                                                                               aCTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  6:37782/1‑140 (MQ=255)
                                                                                                                               aCTACAGACACAA‑CGGCGAACGCATCCAGCCTGAAACTGAGGAGGACTCTATCGGCGGTCAATTCCTACATCTGGTGCACGGCGATAAGCGGTCGCAAAGGTGTGAAAAGGCGAGGCATATATCGCTGGTGCTGTCCGcc              >  8:717585/1‑140 (MQ=255)
                                                                                                                                   cAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGa            >  1:118342/1‑138 (MQ=255)
                                                                                                                                    ggCCCCAC‑CGGCCACCTCATCCAGCCGCATCGTGATGACGACGATATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGaa           <  2:112418/137‑1 (MQ=255)
                                                                                                                                    aGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         >  4:354861/1‑140 (MQ=255)
                                                                                                                                    aGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGAACGGCTAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         >  4:695534/1‑140 (MQ=255)
                                                                                                                                         caA‑CGGCGCACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTTCAGCTGCTGCACGGCGAAAAGCAGTCGCAAAGGTGGGAAAAGGCGCTGCATATCTCGCTGGTGCTGTACGCCGAACACGAgg    >  8:501095/1‑139 (MQ=255)
                                                                                                                                         caA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAACGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGt    >  8:158060/1‑140 (MQ=255)
                                                                                                                                         caA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGt    >  8:1016119/1‑140 (MQ=255)
                                                                                                                                         caA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGt    >  6:512711/1‑140 (MQ=255)
                                                                                                                                         caA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGATGGGAAAAGGCGATGCATATCTCGCTGGAGCTGTACTCCGACCACGAGt    >  4:852524/1‑140 (MQ=255)
                                                                                                                                           a‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCg             <  2:1230673/129‑1 (MQ=255)
                                                                                                                                           a‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGttt  >  4:45734/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
ATGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTT  >  NZ_CP022686/3925150‑3925428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A