Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I0 R1 203 11425.2 8793538 98.4% 8652841 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,925,289 A→C 20.0% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,925,2890AC20.0% 232.7 / 18.4 143N156T (AAC→ACC) prpC2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (79/32);  new base C (3/29);  total (82/61)
Fisher's exact test for biased strand distribution p-value = 2.42e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

ATGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTT  >  NZ_CP022686/3925150‑3925428
                                                                                                                                           |                                                                                                                                            
aTGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGCCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACaa                                                                                                                                              >  8:239461/1‑140 (MQ=255)
aTGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACaa                                                                                                                                              >  8:226604/1‑140 (MQ=255)
   cgcACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAAc                                                                                                                                             <  4:751317/138‑1 (MQ=255)
      aCCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTGTTCTGGTATCACTACCGCCACAACGGCgc                                                                                                                                        >  1:753412/1‑139 (MQ=255)
      aCCGGCGTTTCCGCGCTCGGCGGCACGCTGCCAGAAAAAGAGGGGCCCACCGTTTCTGGTGCGCGGGATATTGCCGACACACTGCTGGCGTCACTTAGTTCGAGTCTTAGCTACTGGTATCACTACAGCACCAACGCCGa                                                                                                                                        >  8:1029204/1‑140 (MQ=255)
       ccGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCg                                                                                                                                         <  4:487379/138‑1 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGa                                                                                                                                        >  3:84494/1‑138 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAAc                                                                                                                                      >  6:502256/1‑140 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTAAAGCCACAACGGCGa                                                                                                                                        >  3:136269/1‑138 (MQ=255)
        cGGCGTTTCCGCGCTCGGCAGCACGCTGCCAGAAAAAGAGGGGCACACCGATTCTGGTGCGCCGGATATAGCCGACACACTGCAGGCGTCACTTAGTTCCATACTTCTCTACGGCTATCACCACAGCCACCACGGCGCAc                                                                                                                                      >  6:236796/1‑140 (MQ=255)
           cGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCa                                                                                                                                   >  6:177576/1‑140 (MQ=255)
           cGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCa                                                                                                                                   >  6:172260/1‑140 (MQ=255)
           cGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTGCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGcgcacgca                                                                                                                                   >  8:383927/1‑140 (MQ=255)
             tttCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATc                                                                                                                                 <  7:226604/140‑1 (MQ=255)
                     cTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑a                                                                                                                        <  1:262174/140‑1 (MQ=255)
                     cTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑a                                                                                                                        >  6:139928/1‑140 (MQ=255)
                       cGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑aaa                                                                                                                      <  1:11405/140‑1 (MQ=255)
                        ggCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCCCTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAGCGCCTCCAGCCGG‑AAAc                                                                                                                     >  7:416259/1‑140 (MQ=255)
                             cACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATACAGCCGG‑AAACtgatg                                                                                                                >  2:472922/1‑140 (MQ=255)
                               ggCTGCCATAAAAATAGGGGCAAACCGTTTCTGGTGCGCGGTATATTGCCTACAAACTGCTGGCGTCACTTTGTTCTATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGT‑AAACTGATgac                                                                                                              <  2:730897/139‑1 (MQ=255)
                               cGCTGCCAGAAAATGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGGTGGCGTCACTTAGTTCGATTCTTCTATACTGGTATCACTACCGCCACACCGGCGAACGCATCCAGCCGG‑AACGTGATgac                                                                                                              <  2:48736/140‑1 (MQ=255)
                               cGCTGCCAGAAAAAGAGGGGAACACCGTTGCTGGGGGGCGGGTTATGGCCTAAAAACTGCTGGGGTAACTTAGTTCGATTAGTCTATACTGGTAACAATACCGCCACACGGGCGAACTAATCCCGCCGT‑AACCTTATgac                                                                                                              <  8:98479/140‑1 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACACCGGCGAACGCATCCAGCCGG‑AAACTGATgacgac                                                                                                           >  5:251717/1‑140 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATgacgac                                                                                                           >  6:821072/1‑140 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTGCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATgacgac                                                                                                           >  7:648767/1‑140 (MQ=255)
                                            agagGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATcggcg                                                                                                 >  7:25159/1‑140 (MQ=255)
                                              gggggCACCCGTTTTCTGGGGCGGGGGATTTTGCCTACAAACGGCTGGCGTCACTTTGTTCTTTTATTCTCTCCTGTTATCACTCCCGCCACACGGGCGAACGCATCCCGCCGT‑AAACTGATGACGACTCTATCGGCGGt                                                                                               <  8:586480/139‑1 (MQ=255)
                                              gggggCACACCGTTTGTGGTGCGCGTGATATTGCCGACAATCTGCTGGCGGCATTTTGTGCTATTCTTCTCTACTGGTATCACTACACCCACAAGGGCGACCGCACCCAGCGGG‑AAACTTTTGACGACTCTATCGGCGGt                                                                                               <  8:733688/139‑1 (MQ=255)
                                              agGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCCGCCGG‑AAACTGATGACGACTCTATcggcggg                                                                                               >  8:481874/1‑139 (MQ=255)
                                                ggccccacCGTGTCTTGGGCGCGGTAAATTCCCTAAACACTGCGGGCTTCACTATGTTCTAATCTTCTCAACTGGAATAACTACAGCCACAACGCCCAACGAATCCCGCCGT‑AAACTGATGACGACTCTATCGGCGGTCa                                                                                             <  2:836912/135‑1 (MQ=255)
                                                  gCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACt                                                                                           >  7:262751/1‑140 (MQ=255)
                                                  gCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCAAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCAcg                                                                                           >  7:867114/1‑139 (MQ=255)
                                                  gCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGAGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCAca                                                                                           >  1:847075/1‑139 (MQ=255)
                                                                gTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGCCGACTCTATCGGCGGTCACTTCCTGCATctgctg                                                                             >  8:975341/1‑140 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCtgctgcc                                                                           >  2:257495/1‑139 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCa                                                                           >  8:393234/1‑140 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTAGATTCTTCTCTACTGGTATCACTACAGCCAAAACGGCGAACGCATCCCGCCGG‑AAACTGATGACGACTCTATCGGCGGGCACTTCCTGCATCTGCTGCa                                                                           >  6:527750/1‑140 (MQ=255)
                                                                    gcgGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTTGTCTCACTACAGCCACACCGGCGAACGCATCCAGCCGC‑AACCTGATGACGACTCTATCGGCGGTCACTTCCTGCATCtgctgcc                                                                           >  4:394289/1‑137 (MQ=255)
                                                                             tGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGc                                                                >  8:342494/1‑140 (MQ=255)
                                                                             ggCCGACAACCTGCTGGCGCCACTTAGTTCGATTCTTCTCTACTGGTACCACTACCCCCACAACGGCTAACGCATCCAGCCGC‑AAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGc                                                                <  1:582490/139‑1 (MQ=255)
                                                                                  ataaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAATACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGCCTCTATCGGCGGTCACTGCCTGCATCTGCTGCACGGGGAAAAGCCGTCg                                                           >  8:969218/3‑140 (MQ=255)
                                                                                  aCAAAGTGCTGGCGTCACTTAGTTCTATTTTTCTCAACTGGTAACACTACACCCACACCGGCTAACGCATCCAGCCGT‑AAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGt                                                             <  3:730164/138‑1 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGCCTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  8:928002/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  2:370202/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  6:13026/1‑140 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  3:526451/1‑138 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCa                                                         >  2:563090/1‑140 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACAACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACTACTCTATCGGCGGTCACTTCCTGCATCTGCTGCCCGGCGAAAAGCCGTCGCa                                                         >  7:869602/1‑140 (MQ=255)
                                                                                    aaaCTGCGGGCGTCACTTAGTTCGATTCTTCTCAACTGGTATCACAACACCCACAACGGCCAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCa                                                         <  8:350514/140‑1 (MQ=255)
                                                                                       ctgcggGCGTCACTTTGTTCGATTCTTCTCTACTGGTATCACACCAGCCACACCGGCGACCGCACCCCCCCGC‑AAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAg                                                      <  1:5006/140‑1 (MQ=255)
                                                                                        ggctgGCGTCACTTAGTTCGATTCTGCTCTCCTGGTATCACTACAGCCACAACGGCGAACGCATCCCGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGc                                                     <  8:29555/139‑1 (MQ=255)
                                                                                                  aCTTCGTTCGATTCTTCTCAACTGGTATCACTACAGCCACACCGGCGAACGCATCCCGCCGG‑AAACTTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGATGGGAAAAgg                                           <  8:946582/140‑1 (MQ=255)
                                                                                                  aCTTAGTTCGATTCTTCTCTCCTGGTAACACTACAGCCACAACGCCGCACGCATCCCGCCCG‑CAACTGATGACGCCTCTATCCGCGGTAGCTTACTGCCTATGCTGCACTGCGAAACGGCGTCGCAAAGCTGGGAACAgg                                           >  8:305716/1‑140 (MQ=255)
                                                                                                  aCTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           >  5:129454/1‑140 (MQ=255)
                                                                                                   tttAGTTCTATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGT‑AAACTTATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGc                                          <  7:293555/139‑1 (MQ=255)
                                                                                                    ttAGTACGATTCTTCTCTCCTGGTATCACACCACCCACACCGGCGAACGCATCCAGCCGG‑AAACTGCTGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCg                                         <  2:263651/140‑1 (MQ=255)
                                                                                                      tgtgcGATTATTCTCTACTGGTATCACTACAGCCACACCGGCTAACGCAACCAGCCGT‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCg                                         <  3:608921/134‑1 (MQ=255)
                                                                                                        ttCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGCTGc                                     >  6:246595/1‑140 (MQ=255)
                                                                                                        ttCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTAAAGGCGGTCACTGACTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGc                                     >  1:832447/1‑140 (MQ=255)
                                                                                                         tCGATTCTTCTCTACTGGTATCACTACAGCCACACCGGCGAACGCATCCCGCCGT‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATg                                      <  4:940571/138‑1 (MQ=255)
                                                                                                            aTTCTTCTCTACTGGTATACCTACAGCCACACCGGGGAACGCATCCCGCCGG‑AACGTGATGACGACTCTATCGGCGGTCTCTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCat                                   <  4:209097/138‑1 (MQ=255)
                                                                                                            aTTCTTATCTACTGGTATAACTACAGCCACAACGGCTAACTCAACCAGCCGT‑AAATTTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  1:595179/140‑1 (MQ=255)
                                                                                                             ttcttcTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTACATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtc                                >  8:125148/1‑140 (MQ=255)
                                                                                                              tcttctCTAGTGGTATCCAGACAGCCAAACCGGCGACCGCAAAACTACGT‑AACCTCATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtct                               <  5:884076/140‑1 (MQ=255)
                                                                                                                 tcttTAGTGTTAACAATACTGCCAGAAGGGCTAACGATTCCTGCGTT‑AAACTTTTCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTTCACGGCGAAAAGCCGTCGCATAGCTGGGAAAAGGCGATGCATATCTCGc                            <  1:1022619/140‑1 (MQ=255)
                                                                                                                 tctcaccTGGTATCAAAACCGCCACACCGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  5:502256/134‑1 (MQ=255)
                                                                                                                 tctcTACTGGTATCACACCCGCCACAACGGCGAACGCATCCCGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  8:747529/140‑1 (MQ=255)
                                                                                                                 tctcTACTGGTATCAAAACAGCCACAACGGCGAACTCAACCCGCCGT‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtctc                              <  4:894595/138‑1 (MQ=255)
                                                                                                                 tctaTACTGGTCACAATCCAGCCACAACGCCGAACGAATCCCGCCGT‑AAACTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtctc                              <  3:549801/138‑1 (MQ=255)
                                                                                                                  ctctACTGGTATCACTACAGCCACAACTGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGTCGATGCATATCTCGCt                           >  2:104618/1‑140 (MQ=255)
                                                                                                                  ctctACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCt                           >  2:893667/1‑140 (MQ=255)
                                                                                                                   tctAGTGGAATCACTCCAGCCACAACGGCTACCGCATCACGCCGG‑AAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTg                          <  5:357142/140‑1 (MQ=255)
                                                                                                                   tcaaCTGGTACCACAACAGCCACAACGGCGAACCCATCCTGCCGT‑AACCTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  3:854665/135‑1 (MQ=255)
                                                                                                                   tcaaCTGGAATCACTACAGCCCCACCGGCGAACGCATCCCCCCGT‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTg                          <  7:51905/137‑1 (MQ=255)
                                                                                                                        tGGTATCACTCCACCCACAACGGCGACCGCACCCCGCCTG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGTAAAGGCGATGCATATCTCGCTGGTGCt                     <  5:177576/140‑1 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAACGGCGAACGCCTCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCCCGGCGAAAAGCCGTCGCAAAGCTGGGAAAAAGCGATGCATATCTCGCTGGTGCTGt                   >  5:73426/1‑140 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGCCTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGt                   >  7:276850/1‑140 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTTCTGt                   >  6:326857/1‑140 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGt                   >  1:38673/1‑140 (MQ=255)
                                                                                                                           aaTCCCTACACCCACACCGGCGCACCCAGCCAGCCGG‑AACCTGCTCACGACTCTCTCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTa                  <  5:437929/139‑1 (MQ=255)
                                                                                                                            aTCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTAc                 >  2:980774/1‑140 (MQ=255)
                                                                                                                            aTCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTAACGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCCTCGCAAAGCTGGGAAAAGTCGATGCATATCTCGCTGGTCCTGTAc                 >  2:769266/1‑140 (MQ=255)
                                                                                                                              cACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCat                                   >  4:951121/1‑120 (MQ=255)
                                                                                                                              cACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTAc                 >  4:184067/1‑138 (MQ=255)
                                                                                                                              cACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTAc                 >  4:915640/1‑138 (MQ=255)
                                                                                                                               aCTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACg                >  4:1083888/1‑138 (MQ=255)
                                                                                                                               aCTACAGCCAAACTGGCGACAGCAACCACCCTT‑AACCTTCTGACGACTCTATCGGATGTAAATTCCTGCATATGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGATGGTGCTGTACGcc              <  2:549314/140‑1 (MQ=255)
                                                                                                                                 tACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGa            >  7:784964/1‑140 (MQ=255)
                                                                                                                                 tACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGG‑AAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGaa           >  2:1096376/1‑140 (MQ=255)
                                                                                                                                  accgCCACACCGGCTAACGCAT‑CACCCGGTAAACTCATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAATGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGaa           <  1:762656/137‑1 (MQ=255)
                                                                                                                                    ccccACAACCGCGACCTCACCAAGCCGG‑AAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGaa           <  3:621884/136‑1 (MQ=255)
                                                                                                                                    aGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         >  5:932929/1‑140 (MQ=255)
                                                                                                                                     gCCACACCGGCGAACCCATCCAGCCGT‑AAACTGTGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAac          <  3:659179/138‑1 (MQ=255)
                                                                                                                                     cccACACCGGCGAACCCATCCACCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAac          <  3:101987/137‑1 (MQ=255)
                                                                                                                                      ccACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGACCACg       >  1:283517/1‑140 (MQ=255)
                                                                                                                                      ccACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACg       >  2:380669/1‑140 (MQ=255)
                                                                                                                                      ccACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTACTGCATCTGCTGCACGGCGAATAGCCGTCGCAAAGCTGGGAAAATGCTCTGCTTATCTCGCTGGTGCTGTACGCCGAgca         >  4:583083/1‑138 (MQ=255)
                                                                                                                                       cacaACGGCGACCGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAacac        >  3:135540/1‑138 (MQ=255)
                                                                                                                                           aCGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGttt  >  7:787674/1‑140 (MQ=255)
                                                                                                                                           aCGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGt    >  4:1039807/1‑138 (MQ=255)
                                                                                                                                           |                                                                                                                                            
ATGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTT  >  NZ_CP022686/3925150‑3925428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A