Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F2 I0 R1 233 6461.8 9239822 98.3% 9082745 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,925,289 A→C 15.6% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,925,2890AC15.6% 288.7 / 10.7 170N156T (AAC→ACC) prpC2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (89/49);  new base C (2/29);  total (91/79)
Fisher's exact test for biased strand distribution p-value = 1.45e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

ATGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTT  >  NZ_CP022686/3925150‑3925428
                                                                                                                                           |                                                                                                                                            
     cACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCg                                                                                                                                         >  1:605773/1‑140 (MQ=255)
      aCCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACCGCCACAACGGCgc                                                                                                                                        >  7:556392/1‑139 (MQ=255)
      aCCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGa                                                                                                                                        >  2:298360/1‑140 (MQ=255)
       ccGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCg                                                                                                                                         <  5:525932/138‑1 (MQ=255)
       ccGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGACACAACGGCGaa                                                                                                                                       <  1:417410/140‑1 (MQ=255)
       ccGGCGTTTCCGCGCGAGTCTTCACCCTGCCAGAATAAGAGGGGCACACCGTTTCTGGTGCGCTGGATAGTGCCTTAAAACTGCTGGCGTCTCTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCg                                                                                                                                         <  6:1024992/138‑1 (MQ=255)
             tttCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCAGACCGTTTCTGGTGCGCGGGTTATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATc                                                                                                                                 <  2:725816/140‑1 (MQ=255)
                ccGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATcc                                                                                                                                <  5:796214/138‑1 (MQ=255)
                  gcgcTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGcc                                                                                                                            >  4:473540/1‑140 (MQ=255)
                     cTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑a                                                                                                                        >  8:264440/1‑140 (MQ=255)
                       cGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑aaa                                                                                                                      <  4:387505/140‑1 (MQ=255)
                       cGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑aaa                                                                                                                      <  1:950244/140‑1 (MQ=255)
                       cGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑aaa                                                                                                                      <  8:534802/140‑1 (MQ=255)
                             cACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACtgatg                                                                                                                >  7:25733/1‑140 (MQ=255)
                               ggCTGCCAGAAAACGAGGGGCACACGGTTTCTGGGGCGGGGTATTTTGCGGAAAAAGTGGTGGCGGCAATTTGTGCGATTTTTCTATACTGGTATCACTACAGCCACAACGGCGAACGCATCCACCCGT‑AAAGTGATgac                                                                                                              <  2:996954/139‑1 (MQ=255)
                               cGCTGCCAGAAAAAGTGGGGCACACCGTTTCTGGGGGGGGGGATTTTGCCTAAAACCTGCTGGCGTCACTTTGTTCTATTCTTCTATACTGTTATCACTACTGCCACAACGGCGAACGCATCCAGCCGT‑AAACtgatg                                                                                                                <  6:861556/138‑1 (MQ=255)
                                gCTGCCAGAAAAAGAGGGGCACCCCGGTTCTGGTGCGCGGGATATTGCCGACAAACTGCGGGCGTCACTTTGTTCGATTCTTCTCTACTGGTAGCACTACAGCCACAACGGCGACCGCAGCCAGCCGG‑CAACTGATgacg                                                                                                             >  3:787167/1‑140 (MQ=255)
                                gCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATgacg                                                                                                             >  1:461270/1‑140 (MQ=255)
                                 cTGCAACAAAAAGAGGGGCACACCGTTTCGGGTGCGCGGTATATTGCCGACAAACTGCTGGCGACACTTAGTTCTATTCTTCTCTACTGGTATCACTACAGCCACAACGGCTAACGCATCCAGCCGG‑AAACTGAGgacga                                                                                                            <  4:615400/140‑1 (MQ=255)
                                  tGCCAGAAAAAGCGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGCCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATgccgac                                                                                                           >  4:859845/1‑140 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACGTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACACCGGCGAACGCATCCAGCCGG‑AAACTGATgacgac                                                                                                           >  3:1101198/1‑140 (MQ=255)
                                    ccAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGTGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCGTCTCTACTGGTATCACGACAGCCACAACGGCGACCGCATCCAGCCGG‑AAACTGATgacgcc                                                                                                           >  6:370550/1‑136 (MQ=255)
                                      aGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTTCCGAAAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACAACAGCCAAAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGActc                                                                                                         >  6:4451/1‑138 (MQ=255)
                                              cggggCACACCTTTTCTGGGGCGCGTGATATTGCCTAAAAACTGCTGGCGTCACTTAGTTCGATTATTCTCTACTGGTATCACAACCCCCCCAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGt                                                                                               <  2:256466/139‑1 (MQ=255)
                                              cgggccccacCGTTTCTGGTGCGCGGGATATTCCGGAAAACCTGCTGGAGTCACTTTGTTCTTTTATTCTATATTGGTATCAATACCGCCACAACGGCGAAGGATTCAAGCCTT‑AAACTTATGACGACTCTATCGGCGGt                                                                                               <  8:507343/133‑1 (MQ=255)
                                              agGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGAGGACGACTCTATcggcggg                                                                                               >  8:70240/1‑139 (MQ=255)
                                                gggCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGCCGCCTCTATCGGCGGTCa                                                                                             >  2:1156101/1‑140 (MQ=255)
                                                 ggCACACCGTTTCTGGTGCGCGGTATATTGCCGACAAACTGCTGGCGTCACTTAGTTCTATTCTTCTCTACTGGTATCACTACAGCCACACCGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCAc                                                                                            <  1:294015/140‑1 (MQ=255)
                                                  gCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACt                                                                                           >  7:106349/1‑140 (MQ=255)
                                                  gCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGGCAca                                                                                           >  1:234498/1‑139 (MQ=255)
                                                     ccccGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACATCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGCCGACGCTATCGGCGGTCCCTTcc                                                                                        >  8:658108/3‑140 (MQ=255)
                                                     cacCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTcc                                                                                        >  1:51005/1‑140 (MQ=255)
                                                      acCGTTTCTGGGGCGCGGTATATTGCCCACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCAACTGGTATCACTACCGCCACACCGGCGAACGCATCCAGCCGG‑AAACTGATCACGACTCTATCGGCGGTCACTTCCt                                                                                       <  7:268916/140‑1 (MQ=255)
                                                        ggTTTCGGGTGCGGGGGATAGTGCCGACAAACTGCGGGCGTCATTTAGTTCGATTCTTCTCTACTGGTATCACTACACCCACACCGGCGAACGCATCCAGCCGT‑AAACTGATGACGACTCTATCGGCGGTCACTTCCt                                                                                       <  6:381064/137‑1 (MQ=255)
                                                          tttCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCCCTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGc                                                                                     >  6:651717/1‑138 (MQ=255)
                                                            tCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATct                                                                                 >  2:428985/1‑140 (MQ=255)
                                                                gTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTATCTACTGGTATCACTACAGCCACCACGTCGAACGCATCCAGCATG‑AAACTGATGACGACTCTATCGGCGGTCACTTCATGCATctgctg                                                                             >  1:951458/1‑140 (MQ=255)
                                                                  gcggGGGATATTGCCGACAACCTGGGGGCGTCATTTAGTGCGATTCTTCTCTACTGGTATCACTACAGCCACACCGGCGAACGCAACCCGCCGT‑AAACTTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCa                                                                           <  2:1020747/140‑1 (MQ=255)
                                                                  gcggGGGATATGGCCGACAAACTGCGGGCGGCACTTAGTTCGTTTCTACTCTCCTGGTATCACTACACCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATctgctg                                                                             <  6:69911/138‑1 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTTGCGTCACTTAGTTCGATTCTTCACTACTGGTATAACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCAACAgcagca                                                                           >  1:1049498/1‑140 (MQ=255)
                                                                  gcgcGGGATATGGCCTAAAAACTGCGGGCGTCACTTTGTTCGATTGTTCTTTACTGGTCTCACTACTCCCAAAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCa                                                                           <  7:648118/140‑1 (MQ=255)
                                                                    gcgGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCATCCGG‑AAACAGATTACGACTCTATCGGCGGTCACTTCCTGCATCAGCTTCa                                                                           >  5:29878/1‑138 (MQ=255)
                                                                    gcgGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCa                                                                           >  5:63799/1‑138 (MQ=255)
                                                                    gcgGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTAGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCa                                                                           >  6:204205/1‑138 (MQ=255)
                                                                     cgtgATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACgg                                                                        >  7:227176/4‑140 (MQ=255)
                                                                     cgGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACgg                                                                        >  3:846192/1‑140 (MQ=255)
                                                                       ggATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACgg                                                                        >  5:280670/1‑138 (MQ=255)
                                                                             ggCCGACACACTGCTGGCGTCCCTTTTTTCGATTATTCTCTACAGCATTAACTCACGCCACATCGGCGAACCCATCCCGCCGC‑AAACTGCGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGc                                                                <  8:578358/139‑1 (MQ=255)
                                                                                  caaacctgctgGCGGACATTAGTGCGATTCTTCTCTCCTGGTTTCACAACACCAACACCGGCTACCGCAGCCACCCGG‑AACCGGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGt                                                             <  6:1034692/133‑1 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTTTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  8:460914/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  8:142177/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  7:84918/1‑140 (MQ=255)
                                                                                    aaaCTGCGGGCGTCACTTAGTGCGATTCTTCTCAACTTGAATCACACCAGCCCCACCGCCGAACGCACCCCGCCGT‑AAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           <  5:889602/138‑1 (MQ=255)
                                                                                    aaaCTGCGGGCGCCACTTCGGGCGATGCTTCTCACCTGGTCCCACCACCCCCACACCGGCTAACCCACCCAGCCGG‑AAACTGAGCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCa                                                         <  7:1027129/140‑1 (MQ=255)
                                                                                       ctccgggCGCCACTTCGTCCGACTCTACCCAACTGGAAACACAACCCCCACACCCGCGAACGCATCCCGCCGC‑AAACTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCaa                                                        <  6:965366/133‑1 (MQ=255)
                                                                                        gggtgccGTCAGATATTTAGATTATTCTAAAGTTGTATCCCTACCCCCACACCGGCTAACGAATCCCCCCGT‑AAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGc                                                     <  7:158874/134‑1 (MQ=255)
                                                                                               gTCACTTAGTTCTATTCTTCTCTCGTGGTACCCATACACCAACAACGGCTAACGCAT‑CATCCGGTAAACTGTGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGa                                                <  5:71490/138‑1 (MQ=255)
                                                                                                  aattAGTTCGATTCTTCTCTACTGTTATCACTACCGCCCCAACGGCGAACCAAACAAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           <  4:444485/138‑1 (MQ=255)
                                                                                                  aattAGTTCGATTCTTCTCTACTGGTATAACTACCGCCACAAGGGCGAACGCATCCAGCCGT‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           <  2:744106/138‑1 (MQ=255)
                                                                                                  aataaGTTCGATTCTTCACTCATGTTATCAATCCAGCCACACCGGAGAACCCAACCAGCCGC‑AAACTTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGaaaa                                             <  5:229328/134‑1 (MQ=255)
                                                                                                  aCTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGCCGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           >  4:163741/1‑140 (MQ=255)
                                                                                                  aCTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCAATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           >  2:616349/1‑140 (MQ=255)
                                                                                                  aCTTAGTTCGATTCTTCTATACTGGTATCACTACCGCCACAACGGCTAACGCATCCAGCCGG‑AAACTTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGaaaa                                             <  5:1116628/138‑1 (MQ=255)
                                                                                                   cTTAGTGGGATTCTTCTCTACTGGTATCACTACACCCACAACGGCGAACGATTCCAGCCGT‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGc                                          <  7:588321/140‑1 (MQ=255)
                                                                                                    ttAGTTGGATTCTTCTATACTGGAAACACTACAGACACACCGGACCACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGGCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCg                                         <  2:136031/140‑1 (MQ=255)
                                                                                                            aTTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 >  7:84242/1‑140 (MQ=255)
                                                                                                            aTTCTTCTCTACTGGTAACCCTACAGCCACACCGGCGACCGCATCCAGCCGG‑AAACTGTTGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  2:506881/140‑1 (MQ=255)
                                                                                                            aTTCTTCTCTACTGGTAACACTACCGCCACACCGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCat                                   <  6:820612/138‑1 (MQ=255)
                                                                                                            aTTCTTCTATACTGTTATCATTACAGCAACCAGGCAGAAGGCTTCCAGCGGC‑AAAGTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  7:547118/140‑1 (MQ=255)
                                                                                                             ttcttcTCTACTGGTATCACTACAGCCACAACGGCGAATGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGTTCACTTCCTGCATCTGCTGCACTGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtc                                >  4:233774/1‑140 (MQ=255)
                                                                                                              tcttctCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 >  6:62414/1‑138 (MQ=255)
                                                                                                              tcttctATACTGGTATCAAAACCGCCCCACGGGCGAACGCATCCCGCGGG‑AAACTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtct                               <  2:33870/140‑1 (MQ=255)
                                                                                                                ttctCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCg                             >  2:1115964/1‑140 (MQ=255)
                                                                                                                ttctCTACTGGTATCACTAAAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtctcc                             >  4:501331/1‑139 (MQ=255)
                                                                                                                 tctcTACTGTTATCACTACCCCCACACCGGCTAACGCATCCAGCGGT‑AAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtctc                              <  6:951906/138‑1 (MQ=255)
                                                                                                                 tctcTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  2:605773/140‑1 (MQ=255)
                                                                                                                 tctcTACTGGTATCACAACACCCACACCGCCGAACGCAACCAGCCGG‑AAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  8:373468/140‑1 (MQ=255)
                                                                                                                 tctcTAATGTTATCACTACCGCAACACGGGCGACCGCAGCCAGCCGC‑AACCTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  3:667345/140‑1 (MQ=255)
                                                                                                                  ctctACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCACAAAGCTGGGAAAAGGCGATGCATATCTCGCt                           >  4:429240/1‑140 (MQ=255)
                                                                                                                      aCTGGTATCACTACAGCCACAACGGCGAACGCAGCCACCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTg                       <  2:1010739/140‑1 (MQ=255)
                                                                                                                         ggTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTg                    >  1:1136095/1‑140 (MQ=255)
                                                                                                                         ggTATCACTACAGCCACAACGGCGAACGCATCCAGACCG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCAGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGAGc                      >  5:718095/1‑138 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGc                                                                >  4:111742/1‑95 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGt                   >  4:106449/1‑140 (MQ=255)
                                                                                                                               aCTACATCCACAACGGCTAACGCATCCAGCCGG‑AAACTGATGACGACTCTATAGGCGGTCACTTCCTGCATCTGCTGCACGACGAAAAGCCGTCGCAAAGCATGGAAAAGGCGATGCATATCTCGCTCGTGCTGTACGcc              >  8:1051525/1‑140 (MQ=255)
                                                                                                                               aCTACAGCCACAACGGCGCACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAACGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  8:275837/1‑140 (MQ=255)
                                                                                                                               aCTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  3:129174/1‑140 (MQ=255)
                                                                                                                               aCTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  3:47046/1‑140 (MQ=255)
                                                                                                                               aCTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCCGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  4:355345/1‑140 (MQ=255)
                                                                                                                                 tACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGTTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAATCTGGGTAAAGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  6:1076800/1‑138 (MQ=255)
                                                                                                                                    cgCCACAACGCCGAACCCATCCCGCCTG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         <  1:772110/139‑1 (MQ=255)
                                                                                                                                    cgCCAAAACGGCTAAGCCATGCAGCCTT‑AAATTGTTGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGATAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATGTCGCTGGTGCTGTACGCCGAaca         <  7:402157/139‑1 (MQ=255)
                                                                                                                                    aGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         >  4:189221/1‑140 (MQ=255)
                                                                                                                                    aGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         >  1:252680/1‑140 (MQ=255)
                                                                                                                                    aGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCATGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         >  4:914051/1‑140 (MQ=255)
                                                                                                                                     gCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAacac        >  1:355253/1‑140 (MQ=255)
                                                                                                                                     gCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCACGCTGGTGCTCTACGCCGAacac        >  4:949811/1‑140 (MQ=255)
                                                                                                                                     gCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGAATATTCTGCACGCCGAAAAGCCGTCGCAAAGCTGGGAAAAGACGATCCATATCTCGCTGGTCCTGTACGCCCAacaa        >  4:770500/1‑139 (MQ=255)
                                                                                                                                     cccACACGGGCGAACGCAGCACCCCGG‑AAAATGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCGGGTGCTGTACGCCGAacac        <  2:321186/139‑1 (MQ=255)
                                                                                                                                     cccACACCGGGCAACCCACCCAGCCGT‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAacac        <  1:194441/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
ATGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAACGGCGAACGCATCCAGCCGG‑AAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTT  >  NZ_CP022686/3925150‑3925428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A