Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F35 I0 R1 304 9232.4 10623078 98.3% 10442485 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,925,289 A→C 13.7% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,925,2890AC13.7% 341.6 / 13.6 186N156T (AAC→ACC) prpC2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (111/45);  new base C (3/27);  total (114/72)
Fisher's exact test for biased strand distribution p-value = 3.05e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

ATGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTT  >  NZ_CP022686/3925150‑3925428
                                                                                                                                           |                                                                                                                                            
aTGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTAGATTCTTCTCTACTGGTATCACTACAGCCACaa                                                                                                                                              >  1:102742/1‑140 (MQ=255)
aTGCGCACCGGCGTTTCCGCGCTCGGCTCCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGACACTTAGTTCGATTGTTCTCTACTGGTATCACTACACCCACaa                                                                                                                                              >  8:1160960/1‑140 (MQ=255)
  gcgcACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCCCACCGTTTCTGGTGAGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATACTTCTCTACTGGTATCACTACAGCCACaa                                                                                                                                              >  3:399828/1‑138 (MQ=255)
  gcgcACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACaa                                                                                                                                              >  4:387075/1‑138 (MQ=255)
     cACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTGTTCTCTTCTGGTATCACTACAGCCCCAA‑CGGCg                                                                                                                                        >  5:1025011/1‑140 (MQ=255)
     cACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCGGGCGTCACGTAGTTCGATTCTGCTCTACTGGTTTCCCTCCTGCGACAA‑CGGCg                                                                                                                                        >  7:821598/1‑140 (MQ=255)
      aCCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGGTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGAGTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGa                                                                                                                                       >  2:155167/1‑140 (MQ=255)
       ccGGCGTTTCCTCGCTCGGCTGCAAGCTGCCATAAAAAGAGGGGCACTCCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGaa                                                                                                                                      <  1:858162/140‑1 (MQ=255)
       ccGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCg                                                                                                                                        <  4:256638/138‑1 (MQ=255)
       ccGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGaa                                                                                                                                      <  2:990054/140‑1 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGa                                                                                                                                       >  4:461215/1‑138 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAAc                                                                                                                                     >  6:1024175/1‑140 (MQ=255)
          gCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGGTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGGCACTTTGTTCGATTCTTCTCTACTGGGATCACTACAGCCACAA‑CGGCGAACGc                                                                                                                                   >  6:286452/1‑140 (MQ=255)
             tttCCGCGGTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACTA‑CGGCGAACGCATc                                                                                                                                <  7:218333/140‑1 (MQ=255)
             tttCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATc                                                                                                                                <  1:471619/140‑1 (MQ=255)
                 cgcgCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTATTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCa                                                                                                                              >  4:1352603/1‑138 (MQ=255)
                  gcgcTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGcc                                                                                                                           <  8:272056/140‑1 (MQ=255)
                  gcgcTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGcc                                                                                                                           <  5:641269/140‑1 (MQ=255)
                     cTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGa                                                                                                                        <  2:943132/140‑1 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAC‑CGGCGAACGCATCCAGCCGGAAACTGATgacgac                                                                                                           >  5:344877/1‑140 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATgacgac                                                                                                           >  2:1039875/1‑140 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATgacgac                                                                                                           >  6:635048/1‑140 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATgacgac                                                                                                           >  2:381043/1‑140 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATCGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAACCTGATcacgac                                                                                                           >  2:513747/1‑140 (MQ=255)
                                    ccAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACACACTGCTGTCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACGGAGgacgcgtc                                                                                                         >  1:1208387/1‑136 (MQ=255)
                                    ccAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATgacgac                                                                                                           >  4:1225693/1‑138 (MQ=255)
                                    ccAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATgacgac                                                                                                           >  4:1120246/1‑138 (MQ=255)
                                       gAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAATCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTAt                                                                                                      >  1:745542/1‑140 (MQ=255)
                                       gAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTATTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTAt                                                                                                      >  8:713868/1‑140 (MQ=255)
                                       gAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTAAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTAt                                                                                                      >  6:807087/1‑140 (MQ=255)
                                            agagGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATcggcg                                                                                                 >  8:306717/1‑140 (MQ=255)
                                            agagGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATcggcg                                                                                                 >  6:244811/1‑140 (MQ=255)
                                              tggggCACACCGTTTCTGGGGCGGGGGATATTGCCGAAAAACTGCTGGCGGCACTTAGTTCGATTCTTCTCTACTGGTATCACTACCGCCACAA‑CGGCGAACGCATCACGCCGGAAACTGAGCACGACTCTATcggcg                                                                                                 <  4:999384/137‑1 (MQ=255)
                                              gggggAACACCTTTTCTGGGGGGCGGTATATTGCCGAAAACCTGCTGGCGTCAATTAGGTCTATTCTTCTCACCTGGTATCACTACACCCACAC‑CGGCTAACGCAGCCAGCCGTAAACTGATGACGACTCTATCGGCGGt                                                                                               <  8:687900/139‑1 (MQ=255)
                                              cggggCACACCTGTGCTGGTGCGCGGGATATTGCCCACAAACTGCTGGCTCCACTAATTTCGTTTCTTATCTACTGGTATAACAACACCCACAA‑CGGCGAACCCATCCAGCCGCAAACTGATGACGACTCTATCGGCGGt                                                                                               <  8:1070817/139‑1 (MQ=255)
                                              cggggCACACCGTTTCTGGTGCGCGGTATATTGCCGACAAACTGCTGGCGTCACTTATTTCTATTGTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCACCCAGCCGTAAACTGAGGACGACTCTATCGGCGGt                                                                                               <  2:559975/139‑1 (MQ=255)
                                                gggCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGt                                                                                               >  4:1107458/1‑138 (MQ=255)
                                                  gCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCAcg                                                                                           >  1:221969/1‑139 (MQ=255)
                                                   caccCCGTTGCTGGTGCGCGGTATATTGCCCACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACACCCGCCACAA‑CGGCTAACGCAGCCAGCCGTAAACTGATGACGACTCTATCGGCGGTCAc                                                                                            <  3:719989/138‑1 (MQ=255)
                                                       ccGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCTCTTCCTg                                                                                      >  7:1123613/1‑140 (MQ=255)
                                                        ggTTTCTGGTGCGCGGTATAGTCCCGACAAACTGCTGGCGGCACTTAGTTCGATAATTCTCTACTGTTATCACAACCGCCACAA‑CGGCGACCGCACCCAGCCGTAAACTGATCACGACTCTATCGGCGGTCACTTCCt                                                                                       <  3:1168885/137‑1 (MQ=255)
                                                        cGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGc                                                                                     >  6:839745/1‑140 (MQ=255)
                                                                gTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATctgctg                                                                             >  8:398121/1‑140 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑Cgg                                                                                                                                          >  8:72678/1‑77 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCCCTTCCTGCATCtgctgcc                                                                           >  5:1132807/1‑139 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATctgctg                                                                             >  4:811801/1‑138 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCAACTGGTATAACTACAGCCCCAA‑CGGCGAACGCATCCAGCCCGAAACTGATTACCACTCAATCGGCGGTCACTTACTGCATcagcagaa                                                                           >  7:618491/1‑136 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTATCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCa                                                                           >  6:956510/1‑140 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCAGGCGTAACTTAGTTCGATTATTAACTACAGGTAACACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGAAGACTCTATCCGCGGTCAATTACTGCCTCAGCTTCa                                                                           >  5:576048/1‑140 (MQ=255)
                                                                  gcgaGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGCACGCATCCAGCCGGAAACTGATGACGACTCTATCTGCGGTCACATCCTGCATAtgctgcc                                                                           >  7:1218053/1‑139 (MQ=255)
                                                                            tggCCGACACACTGCGGGCGTCACTTAGTGCGATTCTCCTCTACTGGTATCACTACCCCCACAC‑CGGCGAACGCATCCAGCCGTAAACTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGaaa                                                                   <  4:611151/136‑1 (MQ=255)
                                                                             tGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGc                                                                >  8:347881/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  1:398364/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  8:475290/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTATTATCTACTGGTATCACTACAGCCACAA‑CGGCGAACGAATCCAGCCGGAAACTGATGACGCCTCTATCGGCGTTCACTTCCTGCATCTGCTGCACTGCGAAAAGCCGTCg                                                           >  2:13339/1‑140 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  3:1037888/1‑138 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  3:470754/1‑138 (MQ=255)
                                                                                              cGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTggg                                                 >  4:420045/1‑138 (MQ=255)
                                                                                                  acttacttCTATTATTCTCAACTGGTATCACTACACCCACAC‑CCGCGAACGCAACCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           <  2:640462/140‑1 (MQ=255)
                                                                                                  aCTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           >  2:1064992/1‑140 (MQ=255)
                                                                                                    ttATTTCTATTCTTCTCTACTTGTATCACTACAGCCACAA‑CGGCTAACCCATCCCGCCGTAAACTTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           <  4:1032661/138‑1 (MQ=255)
                                                                                                       gTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATg                                      >  1:343627/1‑140 (MQ=255)
                                                                                                       gTTCGATTCTTCACTACTGTAATCAAAACCCCCAAAA‑CGGCGACCGCACCCAGCGGTAAACTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATg                                      <  1:422711/140‑1 (MQ=255)
                                                                                                        ttCGATTCTTCTCTACTGGTATCACTACAGCCACAC‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGc                                     >  6:762038/1‑140 (MQ=255)
                                                                                                            tttcttcTCTACTGGTATCACTACCGCCACAC‑GGGCTAACGCATCCAGCCTTAAACTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  5:1222197/139‑1 (MQ=255)
                                                                                                            tttcttcTCTACTGGTATCACTACAGCCCCAA‑CGGCTAACGCATCCAGCCGGAAACTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  5:80073/139‑1 (MQ=255)
                                                                                                            tttcttcTCTACTGGTATCACAACACCAACAA‑CGGCTAACGCATCCAGCCGTAAACTTATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  2:398364/139‑1 (MQ=255)
                                                                                                             ttcttcTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTACTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtc                                >  6:481265/1‑140 (MQ=255)
                                                                                                              tcttctCTACTGGTATCACTACCGCCCCAC‑CGGCGAACGCATCCCCCCGCAAACTCATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtct                               <  8:620741/140‑1 (MQ=255)
                                                                                                                 tctctcCTGGTATCACTACAGCCCCAC‑CGGCGAACGCACCCCGCCGCAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  7:492212/140‑1 (MQ=255)
                                                                                                                 tctctcCTGGAAACACACCAGCCCAAA‑CGCCTAACCCAGCCCGCCGCAAACGTAGCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  5:870139/140‑1 (MQ=255)
                                                                                                                 tctcTAGTGTTATCACAACA‑CCACAACCGCCTAACTCATCCCGCCGTAAACTCAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATAtctc                              <  3:20265/138‑1 (MQ=255)
                                                                                                                 tctcTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            >  2:39519/1‑140 (MQ=255)
                                                                                                                 tctcTACTGGAATCACAACCCCCACAC‑CGGCCAACGCATCCAGCCGGAAACTTATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  7:52875/140‑1 (MQ=255)
                                                                                                                  ctctACTGGTATCACTACAGCCACAA‑CTGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGACGTAGCAAAGCTGGGAAAAGGCTATGCATATATCgat                           >  8:111610/1‑138 (MQ=255)
                                                                                                                  ctctACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCt                           >  1:1276803/1‑140 (MQ=255)
                                                                                                                   tctACTGGTATCACTACAGCCAAAA‑CGGCTACCGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTg                          <  7:316801/140‑1 (MQ=255)
                                                                                                                   tctACGGGAATCACAACACCCACAA‑CGGCGACCGCAGCAAGCCGGAAACTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTg                          <  1:1020978/140‑1 (MQ=255)
                                                                                                                      agtGGTAACAATACAGCCACAC‑CGGCTAACGCATCCTGCCGTAAAGTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTgg                         <  4:874522/136‑1 (MQ=255)
                                                                                                                      aCTGGTATCAATACAGCCACAC‑CGGCGAACGCATCCAGCCGGAAAGTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTg                       <  6:408965/140‑1 (MQ=255)
                                                                                                                          gTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGt                   >  8:85553/1‑140 (MQ=255)
                                                                                                                            aTCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAATCCGTCGCATAGCTGGGAAAAGGCGATGCATATCTCGCTGTTGCTGTAc                 >  2:1242399/1‑140 (MQ=255)
                                                                                                                            aTCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGGTGCATATCTCGCTGGTGCTGTAc                 >  7:94548/1‑140 (MQ=255)
                                                                                                                              cACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTAc                 >  4:324663/1‑138 (MQ=255)
                                                                                                                                 tACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGa            >  7:906269/1‑140 (MQ=255)
                                                                                                                                  cctcccACAC‑CGGCCAACGCAGCCTCCCGTAACCTGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTAGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCg             <  3:272085/134‑1 (MQ=255)
                                                                                                                                    cgCCACAA‑GGGATAACCCATCCAGCCGTAACGTCTTGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGTAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         <  8:612196/139‑1 (MQ=255)
                                                                                                                                    aGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGaccc         >  6:1108128/1‑137 (MQ=255)
                                                                                                                                    aGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGAGATGCATATCTCGCTGGTGCTGTACGCCGAaca         >  8:897113/1‑140 (MQ=255)
                                                                                                                                     cccACAC‑CGGCTAACGAATCCAGCCGTAAAGTCATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAacac        <  6:1059388/139‑1 (MQ=255)
                                                                                                                                      ccACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTTGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         >  3:608586/1‑138 (MQ=255)
                                                                                                                                      ccACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAacacc       >  1:1330343/1‑139 (MQ=255)
                                                                                                                                      ccACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGACGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         >  4:870261/1‑138 (MQ=255)
                                                                                                                                        acaA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACg       >  3:1272544/1‑138 (MQ=255)
                                                                                                                                         caA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATTCATATCTCGCTGGTGCTGTACGCCGAACACGAGt    >  1:370915/1‑140 (MQ=255)
                                                                                                                                          aa‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGtt   >  1:1159220/1‑140 (MQ=255)
                                                                                                                                           a‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGATAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGttt  >  1:1035787/1‑140 (MQ=255)
                                                                                                                                           a‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGt    >  3:901371/1‑138 (MQ=255)
                                                                                                                                           a‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGt    >  4:437105/1‑138 (MQ=255)
                                                                                                                                           a‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGAAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGttt  >  2:1041679/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
ATGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCACTACAGCCACAA‑CGGCGAACGCATCCAGCCGGAAACTGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTT  >  NZ_CP022686/3925150‑3925428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A