Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F2 I0 R1 64 5414.3 5027226 98.2% 4936735 134.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,954,923 T→G 13.9% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,954,9230TG13.9% 134.0 / 3.9 93T92P (ACG→CCG) ttdRDNA‑binding transcriptional activator TtdR
Reads supporting (aligned to +/- strand):  ref base T (31/47);  new base G (13/2);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 1.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGC  >  NZ_CP022686/1954787‑1955056
                                                                                                                                        |                                                                                                                                       
tCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGtt                                                                                                                                        >  7:195093/1‑138 (MQ=255)
tCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTACGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGtt                                                                                                                                        >  7:188722/1‑138 (MQ=255)
   aTTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTg                                                                                                                                     <  4:79349/138‑1 (MQ=255)
     ttGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTgtt                                                                                                                                   >  1:573558/1‑136 (MQ=255)
     ttGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑t                                                                                                                                  >  2:557410/1‑138 (MQ=255)
     ttGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑t                                                                                                                                  >  2:566856/1‑138 (MQ=255)
     ttGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑t                                                                                                                                  >  7:452170/1‑138 (MQ=255)
        tcgatcgaACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACAAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTg                                                                                                                               >  4:416720/1‑138 (MQ=255)
          gatcgaACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTTA‑TTTTCg                                                                                                                             >  3:554430/1‑138 (MQ=255)
          gatcgaACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGCCGCAATATGGCTGCGACCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCg                                                                                                                             >  7:446709/1‑138 (MQ=255)
                 cAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGAGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTc                                                                                                                      <  7:299854/138‑1 (MQ=255)
                 cAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCAGCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTc                                                                                                                      <  2:539236/138‑1 (MQ=255)
                     tCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCTCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCg                                                                                                                  <  1:546223/138‑1 (MQ=255)
                     tCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCg                                                                                                                  <  1:566856/138‑1 (MQ=255)
                     tCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCg                                                                                                                  <  3:47722/138‑1 (MQ=255)
                     tCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGAc                                                                                                                <  6:316537/140‑1 (MQ=255)
                       aaaaTGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGAc                                                                                                                <  7:271295/138‑1 (MQ=255)
                         aaTGCACCTGTAACTCAGGATAATTGCGCATCAGGTCGGTCATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCACTGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGt                                                                                                            <  5:429752/140‑1 (MQ=255)
                           tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTTCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGt                                                                                                            <  1:326431/138‑1 (MQ=255)
                           tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGt                                                                                                            <  4:494862/138‑1 (MQ=255)
                           tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGt                                                                                                            <  4:2316/138‑1 (MQ=255)
                           tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATCCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGt                                                                                                            <  1:194017/138‑1 (MQ=255)
                              aCCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGA‑TTTGGCTGACGTAATCGCCCAGTCGcgg                                                                                                       >  6:556492/1‑138 (MQ=255)
                                cTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGTCTCATCCCATCCGGGCGCGTTTTTT‑TTTGCTTGACGTAATAGCACAGTCGCTg                                                                                                       >  8:381011/1‑138 (MQ=255)
                                cTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGA‑TTTGCGTGGCGTCATCGCCCAGTCgggg                                                                                                       >  2:281946/1‑135 (MQ=255)
                                         aGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑ATTGCGTGACCTCATCGACCAGTCGCTGA‑TATTGGGt                                                                                             >  4:31581/1‑138 (MQ=255)
                                          ggATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCCGTCGCTGA‑TATTGGGGGAg                                                                                          >  6:349934/1‑140 (MQ=255)
                                           gATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTTA‑TTTGGTTGGCGTCATCGGCCAGGAGCTGG‑TTTTGGGTgg                                                                                           >  1:631828/1‑137 (MQ=255)
                                            aTAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGAGTGACGTCATAGACCACTAGCAGA‑TATTtgggtag                                                                                          >  3:552712/1‑134 (MQ=255)
                                            aTAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGACCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTGTTT‑TTTGCGGGACGTCATCGACCAGTCACTGA‑TATTGGGGGat                                                                                          >  7:40464/1‑137 (MQ=255)
                                              aaTTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGa                                                                                        <  8:52623/138‑1 (MQ=255)
                                                ttGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGAtt                                                                                      <  8:318317/138‑1 (MQ=255)
                                                ttGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGAtt                                                                                      <  7:174012/138‑1 (MQ=255)
                                                  gcgcATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTc                                                                                    <  8:321111/138‑1 (MQ=255)
                                                     cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAACACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTTC‑TTTGCGTGCCGTGATCTACCAGTCTCGGC‑TATTGTGTGAGGATTTCCAg                                                                                 >  3:214986/1‑138 (MQ=255)
                                                     cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTTATTTTGGGTG‑CGTCATCCACCCGTCGGGGG‑ATTTGGGGGAGGAtttaacgcg                                                                               >  5:68942/1‑134 (MQ=255)
                                                     cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTTA‑TTTTGGTGGCGTCATAGGCCAGTCGCGGATTATTGGG‑GAGGATTTTCatcc                                                                               >  5:36448/1‑137 (MQ=255)
                                                     cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTTCGTGACGTCATCGACCAGGCGCTGA‑TATTGGGTGAGGATTTCAAgcg                                                                               >  6:305140/1‑140 (MQ=255)
                                                      aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAgcgc                                                                              <  5:188375/140‑1 (MQ=255)
                                                      aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAgc                                                                                <  7:333594/138‑1 (MQ=255)
                                                      aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCGGA‑TATTGGGTGAGGATTTCCAgcgc                                                                              <  6:511044/140‑1 (MQ=255)
                                                       tCAGTTCGGTAATAGCTTGCGCAATATGGCTGCGCCCAACACCAAAACTGCAACCAATGCGGCTCATCCCCTCCGGGCGCGTGTTGA‑TTTGCGTGACGTCATCGACCCGTGGCGGC‑TATTGGGTGTCGATTTCCacgc                                                                               >  8:543853/1‑135 (MQ=255)
                                                       tCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAgcg                                                                               >  7:246618/1‑138 (MQ=255)
                                                       tCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATAGACCAGTCGCTGA‑TATTTGGTGAGGATTTCCAtcg                                                                               >  3:375104/1‑138 (MQ=255)
                                                       tCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGA‑TTTGGGTGGCCTCATCGACCAGTCGGGGA‑GAATGGGTGGGGGTTTACAgcg                                                                               >  1:150003/1‑138 (MQ=255)
                                                          gTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTTGGTGACGTAATCGACCAGTCGCTGA‑TATTTGGTGAGGATTTACAGCGCAt                                                                            >  1:450035/1‑138 (MQ=255)
                                                          gTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTTA‑TTTGGGTGTCGTGATAGACCAGGCGGGGA‑TATTGGGTGGGGGTTTGCAggggat                                                                            >  1:446989/1‑133 (MQ=255)
                                                           ttCGTTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATg                                                                           <  2:298833/138‑1 (MQ=255)
                                                           ttCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATAATCCCCTCCGGGCGCGTTTTGA‑TTTGCTTGACGTCATAGACCAGTCGCTGA‑TATTGTGTGAGGATTTCCAGCGCATGtt                                                                         <  5:314408/140‑1 (MQ=255)
                                                           ttCGGTAATAGCTGGCGCAATATGGATGCGACAAAAACCAAAACTGCAACCAAAGCGGATCATCCCCTCCGGGCGCGGTTTGA‑TATGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGtt                                                                         <  6:374288/140‑1 (MQ=255)
                                                             cGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTTT‑TTTTGGTGTCGTCATCGACCAGTCGGAGT‑AATTGGGGGAGGGTTTGCACCGCATGTTCg                                                                       >  5:191953/1‑140 (MQ=255)
                                                                  aTAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGT‑TTTGCGTGACGTCATCGACCAGTGGCGGA‑TTTTTGGGGTGGGTTTGCACGGGATGTTTGTtaca                                                                  >  5:116005/1‑140 (MQ=255)
                                                                    aGCTGGCGCAATATGGCTGCGCTCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGGGACGACATCGACAACTCGCTGA‑TATTGTGGGTGGCTTTCCAGCGCATGTTGGTaaca                                                                  >  3:450742/1‑138 (MQ=255)
                                                                       tGGCGCAATATGGCTGCGCCCAACACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑GTTGGGTGGCGTAATCGACCCGTCGGAGA‑TTATGGGTGTGGATTTCCTGCGCATGTTCGTTACAACg                                                               >  2:55419/1‑138 (MQ=255)
                                                                          cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCTGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                          <  5:496701/140‑1 (MQ=255)
                                                                          cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGGCGTCATCGCACACTCGCTGA‑TATTGGGTGAGGATTTCCCGCGCATGGTAGTAACAACGCTGGc                                                          >  6:74940/1‑140 (MQ=255)
                                                                          cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                          <  5:589144/140‑1 (MQ=255)
                                                                          cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                          <  5:249808/140‑1 (MQ=255)
                                                                          cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGGGACGTCATCGACCAGTGGCTGA‑TATTGGGTGAGGATTTGCAGCGCATGTTGGTAAAAACCGTGGc                                                          >  6:109130/1‑140 (MQ=255)
                                                                          cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGA‑TTTGGGGGGCGTCATCGGCCAGTCGGGGG‑TATTGGGGGTGGTTTTCCAGCGCATGGTGGTTACAACCCTg                                                            >  4:179207/1‑138 (MQ=255)
                                                                          cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGA‑TTTGCGGGACGGCATCGACCAGTCGCGGT‑GATTTGGGGAGGGTTTCCAGCGCATGTTAGTAACAACGCTg                                                            >  3:473352/1‑138 (MQ=255)
                                                                          cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTACGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTg                                                            <  8:337928/138‑1 (MQ=255)
                                                                            cAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGA‑TTTGCGGGGCGTCCTCGCCCAGGCGGGGA‑TATTGGGTGAGGTTTTACAGCGCATGTTGGTTACAACGCTggg                                                          >  3:511058/1‑137 (MQ=255)
                                                                                atGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACt                                                      <  8:246618/138‑1 (MQ=255)
                                                                                    cTGCGCCCAAAACCAAAACTGCAACCACTTCGGATCATACCCTCCGGTCGCGTTGAGA‑TTTGCGTGACGTCATCTACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTtca                                                  <  7:376492/138‑1 (MQ=255)
                                                                                    cTGCGCCCAAAACCAAAACTGCAACCAATGCTGATCATCCACTCCGTGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTtca                                                  <  7:484847/138‑1 (MQ=255)
                                                                                     tGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGT‑TTTTGGTGACGTCATCGGCCAGTCGCGGG‑TATTGGGTGTGGGTTTACAGCGCATGTTGGTTACAACCCTGGCCACGTtaag                                                 >  1:447038/1‑138 (MQ=255)
                                                                                      gcgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTACTCGCCCAGTCGCTGA‑TATTGGGTGAGGAATTACAGCGCAAGTTGGTAACAACGCGGGCCCCGTtcagt                                                >  4:334830/1‑138 (MQ=255)
                                                                                         cccAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGGCGCTGA‑TATTGGGTGAGGATTTCCAGCGCAAGTTCGTAACAACGCGGGCCACTTtcagtcag                                             >  2:448204/1‑138 (MQ=255)
                                                                                         cccAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCAGGGCGCGTTTTTA‑ATTTCGTGACGTCATCGACCAGTCGCTGA‑TAATGGGTGAGGATTTACAGCGCATGTTGGTAACAACCCTGGCCACATtaagtcac                                             >  4:193949/1‑137 (MQ=255)
                                                                                           caaaaccaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGGTTGA‑TTTTCGTGACGTCATCGCCCAGACGAAGA‑AATTTGGGGGAGATTTGCACCCCATGTTCGTTAAAACGCTGGCCACATTAAGGCAacg                                           >  2:199991/1‑138 (MQ=255)
                                                                                               accaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCCGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCAc                                       <  8:551368/138‑1 (MQ=255)
                                                                                                     acTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATAGCCCACTCGCTGA‑TATTTGGTGAGGGTTTACAGCGCATGTTCGTTAAAACGCTGGCCCCATTCAGTCAGCGCCACCCcgcg                                 >  2:202389/1‑138 (MQ=255)
                                                                                                        gCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTATCAGTCAGCGCCACGCCGCgagc                              <  4:620848/138‑1 (MQ=255)
                                                                                                          aaacAATGCGGATCATCCCCTCCGTGCGCGTTTTGA‑TTAGCGTGACGTCATCTACCAGTCGCAGA‑TATTGGGTGAGGATTTCCAGCGCATGTGCGTAACAACGCGGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                            <  2:161818/135‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCTTCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                            <  8:86295/138‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCCTCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                            <  4:47778/138‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTTGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                          <  6:318874/140‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                          <  5:360523/140‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                          <  5:312886/140‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                            <  7:219582/138‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                            <  8:102938/138‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                            <  8:551636/138‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGGGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                            <  7:52169/138‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCCTCCGGGCGCGGTTTGA‑TTTGCGTGACGTCATCGACCAGGCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                            <  2:277579/138‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCCCATCCGGGCGCGTTGTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                            <  2:6854/138‑1 (MQ=255)
                                                                                                          aaCCAATGCGGATCATCACCTCCGTGCGCGTTTTGA‑TTTGCGGGACGTCATCGACCAGACGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGATCGTAACAACGCTGGCAACTTTCCGTCAGCGCCACGCCGCgagcgg                            <  8:82557/138‑1 (MQ=255)
                                                                                                           aCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGCCCAGGCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAAAAACGCTGGCCACTTTCAGTCAGCGCCACCCCGCgcgcggg                           >  2:392258/1‑137 (MQ=255)
                                                                                                             cAATGCGGATCATGCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggagc                         <  8:254280/138‑1 (MQ=255)
                                                                                                             aaaTGCGGATCCTCCCCTCCGGGCGCGTTTTGA‑TTTGCGGGACGTCATCGACCTGTCGCTGA‑TATTGGGGGAGGATTTCCAGCGCATGTTCGTAACAACGCGGGCCACTTTCAGGCAGCGCCACGCCGCtagcggagc                         <  8:322727/137‑1 (MQ=255)
                                                                                                                    gATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGTCCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGATCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGAGGAGCGGTTcagca                <  5:458459/140‑1 (MQ=255)
                                                                                                                    gATCATCCCCTCCGGGCGCGTTGTGA‑TTTGCCTGACGGCATCGCCCAGTCTCGGA‑GATTGGGTGAGGATTTCCACCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcag                  <  1:533340/138‑1 (MQ=255)
                                                                                                                                 gggCGCGTTTTGA‑TTTGGGTGACGTGATCGACCAGTCGCTGA‑TATTTGGTGTGGATTTACAGCGCATGTTCGTAACAACGCTGGCCACTTTAAGTAAGCGCCCCGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGcc     >  2:40074/1‑138 (MQ=255)
                                                                                                                                  ggCGCGTTTTTC‑TTTGGGTGAAGTCATCGACCAGGCGGTGA‑TATTGGGTGAGGATTTCCCGCGGATGTTGGTAAAAACCCTGGCCCCTGTGCGTGCGCGCCACCCCGCCAGCGGAGCGGTTGAGCAGAGGTGTTGCGAGc  >  6:460239/1‑140 (MQ=255)
                                                                                                                                    cgcgTTTTGA‑TTTTCGTGACGTCATCGACCAGTCGGGGA‑TATTTGGTGAGGTTTTCCCGCGCATGTTAGTTACAAACCGGGGCAATTTAAGTAACCGCCCCGCCGCGAGCGGAGCGGGTCAGCCGAGgtgttggccgc  >  8:508865/1‑133 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGA‑TTTGCGTGACGTCATCGACCAGTCGCTGA‑TATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGC  >  NZ_CP022686/1954787‑1955056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A