Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F36 I0 R1	179	9550.8	8038540	98.3%	7901884	136.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	1,954,923	T→G	19.9%	T92P (ACG→CCG)	ttdR ←	DNA‑binding transcriptional activator TtdR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	1,954,923	0	T	G	19.9%	204.3 / 21.3	142	T92P (ACG→CCG)	ttdR	DNA‑binding transcriptional activator TtdR
Reads supporting (aligned to +/- strand): ref base T (35/76); new base G (28/3); total (63/79)
Fisher's exact test for biased strand distribution p-value = 3.28e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

AATCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCC  >  NZ_CP022686/1954785‑1955053

aaTCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGtt                                                                                                                                    >  1:536490/1‑140 (MQ=255)

     aTTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGAt                                                                                                                               <  8:254078/140‑1 (MQ=255)

        tGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTTAtttt                                                                                                                            >  3:854710/1‑139 (MQ=255)

            gatcgaACAATTCAAAATGCACCTGTGACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTg                                                                                                                        <  5:365798/140‑1 (MQ=255)

            gatcgaACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTg                                                                                                                        <  3:212668/140‑1 (MQ=255)

             atcgaACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGa                                                                                                                       <  4:895546/140‑1 (MQ=255)

                   cAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCAt                                                                                                                 <  5:828536/140‑1 (MQ=255)

                    aaTTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACCTCATc                                                                                                                >  1:250533/1‑140 (MQ=255)

                       tCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGAc                                                                                                             <  7:570822/140‑1 (MQ=255)

                       tCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGAc                                                                                                             <  4:328228/140‑1 (MQ=255)

                       tCAAAATGCAACTGTAACTCAGGATAATTGCGAATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGAGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGAc                                                                                                             <  8:927540/140‑1 (MQ=255)

                        cAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGAcc                                                                                                            >  1:144793/1‑140 (MQ=255)

                         aaaaTGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCa                                                                                                           <  6:58134/140‑1 (MQ=255)

                           attGCTCCTGTAACTAAGGATAATTGCGCATCAGTTCGGTAATAGCTGGGGAAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGTCCAGt                                                                                                         <  7:700508/138‑1 (MQ=255)

                           aaTGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGt                                                                                                         <  1:1037724/140‑1 (MQ=255)

                           aaTGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGt                                                                                                         <  2:72535/140‑1 (MQ=255)

                             ttcACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCg                                                                                                       <  4:152731/138‑1 (MQ=255)

                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTTATTTGCGTGACGTCATCGACCAGtag                                                                                                       <  6:632589/140‑3 (MQ=255)

                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCg                                                                                                       <  2:144793/140‑1 (MQ=255)

                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCg                                                                                                       <  7:881822/140‑1 (MQ=255)

                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCg                                                                                                       <  7:521634/140‑1 (MQ=255)

                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCg                                                                                                       <  5:118072/140‑1 (MQ=255)

                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCg                                                                                                       <  3:974760/140‑1 (MQ=255)

                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCg                                                                                                       <  3:855538/140‑1 (MQ=255)

                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCg                                                                                                       <  3:345888/140‑1 (MQ=255)

                              gCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGATGAGACCAAACCCAAAACTGCAACCAATGCGGATCAACCCCTCCGGGCGCGTTTTGAATTTCCTGAACTCATCGACCatgtca                                                                                                      >  2:650099/1‑135 (MQ=255)

                                aCCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGGGTTTTGTTTTGGGTGAAGTCAGAGACCCGTCGccg                                                                                                    >  1:822263/1‑138 (MQ=255)

                                    gTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGatat                                                                                                <  1:863664/140‑1 (MQ=255)

                                     tAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACCTAATCGCCCAGTCGCTGATAtt                                                                                               >  4:234064/1‑140 (MQ=255)

                                     tAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGGGTGACGTCATCGCCCAGTCGCGGCTAtt                                                                                               >  8:432793/1‑140 (MQ=255)

                                     tAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGACGTCATCGACCCGTCGCTGATAtt                                                                                               >  4:935115/1‑140 (MQ=255)

                                        cTCAGGATAATTGCTCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTggg                                                                                            <  8:532975/140‑1 (MQ=255)

                                        cTCAGGATAATTGCGCAGCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTggg                                                                                            <  5:466419/140‑1 (MQ=255)

                                          cAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTg                                                                                          <  3:598214/140‑1 (MQ=255)

                                            ggATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTTATTTGCGTGAAGTCATAGACCAGTAGCAGAAATTTGGTG‑Ag                                                                                       >  3:457244/1‑140 (MQ=255)

                                            ggATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTGTTTATTTGCGTGTCGTCATCGGCCCGTCGCGGATATTtggggtg                                                                                        >  4:154578/1‑136 (MQ=255)

                                            ggATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTTAGTTGAGTGTCGTCATAGACCAATCGCGGATATTgggggcg                                                                                        >  5:297654/1‑136 (MQ=255)

                                            ggATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGGTTGATTTGGGTGACGTCATCGACCAGTCGCGGATTTTGGGTggg                                                                                        >  5:693805/1‑138 (MQ=255)

                                                aaTTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGAtt                                                                                   <  4:425031/140‑1 (MQ=255)

                                                  ttGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGTTTTGCGTGACGTCAAAGACAAGTCGCTGTTTTTTGGTG‑AGGAttta                                                                                 >  5:683051/1‑139 (MQ=255)

                                                  ttGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTc                                                                                 <  3:912375/140‑1 (MQ=255)

                                                  ttGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGGG‑AGGATTTc                                                                                 <  8:469243/140‑1 (MQ=255)

                                                       cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCg                                                                                                       >  8:364964/1‑114 (MQ=255)

                                                       cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCg                                                                                                       <  7:364964/114‑1 (MQ=255)

                                                       cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGATGGCCCTCCTCCCCCATGGGCTGAAAATGGGTG‑AGGATTTCCatca                                                                            >  3:922367/1‑137 (MQ=255)

                                                       cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTTGGTGAAGTCAACGACCCCGCGCGGGTATTGTGTG‑TGGAtttaaagcc                                                                            >  7:848545/1‑134 (MQ=255)

                                                       cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGACGTCCGAGGCCAGTCGGAGGTATTTGGTGCAGGAttgcagcg                                                                             >  7:341703/1‑134 (MQ=255)

                                                       cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGACGTCAACGCACAGTCGCTGATATTTGGTG‑ATGATTTCCAgcc                                                                            >  4:159475/1‑139 (MQ=255)

                                                        aTCAGTTCGGTACTAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGAGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAgcgc                                                                           <  2:973551/140‑1 (MQ=255)

                                                        aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAACCTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAgcgc                                                                           <  5:592625/140‑1 (MQ=255)

                                                        aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAgcgc                                                                           <  2:596676/140‑1 (MQ=255)

                                                        aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAgcgc                                                                           <  4:450988/140‑1 (MQ=255)

                                                                  tAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATAGACCAGTCGCTGATATTGGGTG‑TGGATTTCCAGCGCCTggtggtta                                                                 >  4:49008/1‑133 (MQ=255)

                                                                     tAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGCCGTCATCGACCAGTCGCAGGTTTTTGGTG‑TGGATTTGCAGCGCATGTTGGTaacaa                                                              >  3:63212/1‑140 (MQ=255)

                                                                     tAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGCCGTCAACGACCAGTCGGGGATATTGGGGG‑AGGAGTTACCCCCGATGTTGGTaacaa                                                              >  2:5020/1‑140 (MQ=255)

                                                                       gCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCACCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTTACGTCATGGCCCAGGAGCTGATATTGGGGG‑AGGAGTTACAGCGCGTGTTCGTtacaacc                                                            >  4:71086/1‑139 (MQ=255)

                                                                       gCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACg                                                            <  5:981080/140‑1 (MQ=255)

                                                                       gCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGGGACGTCAGCGACCAGTCGCTGATATTGTGTG‑AGGATTTACATCGCAGTTTCGTaaaaacc                                                            >  6:910905/1‑139 (MQ=255)

                                                                           gcgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCTATGCGGATCATCCCCTCCGGGAGCGGTTTTATATTCGTGACCTCATAGAACAGTCGATGGTATTGGGTG‑ATGAATTACAACGCCTGTTCCTAAAAACCCtcg                                                        >  8:422217/1‑138 (MQ=255)

                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTTCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTTGTTTGCGTGACCTCATAGCCCAGTCGCAGATTTTTGGTG‑CGGATTTACAGCGAATGTTAGTAACAACGCAGGc                                                       >  8:132185/1‑140 (MQ=255)

                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGCTTTCCAGCGCATGTTCGTAACAACGCTGGc                                                       >  8:635088/1‑140 (MQ=255)

                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                       <  2:445368/140‑1 (MQ=255)

                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                       <  1:813234/140‑1 (MQ=255)

                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTGTTGATTTGCGTGTCGGGCTCGACCAGTCGCGGATTTTGGGGG‑TGGTTTTACAGCCCATGTTGGTAACAACGCTGGc                                                       >  1:965163/1‑140 (MQ=255)

                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGACGTCATAGACCAGGCGCGGCTATTTGGTG‑GGGATTTCCAGCGCAAGTTAGTAACAACCCGGGc                                                       >  8:595448/1‑140 (MQ=255)

                                                                                atctcgCTGCGCCCAAAACCAAAACTGAAACCAATGCGGATCATCCCCTCCGGGCGCGGTGTGATTTGAGTTAAGTAATAGACCAGGAGCTGATATTGGGTG‑AGGATTTACAGCGAATGTTCGTAAAAACACTTGCCaat                                                   >  8:593725/6‑138 (MQ=255)

                                                                                         cgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTTCGTGACGTCATAGACCAGTAGCTGATATTGGGTG‑AGGATTTACAGCGCATGTTAGTAACAACGCTGGCCCCATtaagtcaa                                          >  6:903075/1‑139 (MQ=255)

                                                                                         cgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGGCCAGGCGCGGATTTTGGGTG‑AGGATTTGCAGCGCATGTTCGTAACAAACCTGGCCCCTTtcagtgag                                          >  7:430123/1‑140 (MQ=255)

                                                                                         cgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGCCCAGTCGCTGATATTGGGTG‑AGGAGTTCCAGCGCATGTTCGTAACAACCCTGGCCACTTtcagtccg                                          >  2:296095/1‑138 (MQ=255)

                                                                                         cgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCGGATATTGGGTG‑AGGATTTACAGCGCATGTTCGTAACAACGCTGGCCACTTtcagtcag                                          >  3:229846/1‑140 (MQ=255)

                                                                                           cccAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGGTTTGCGTGGCGTCCTCGCCCAGGCGCTGATTTTGGGGG‑AGGGTTTACGGCGCCTGTTCGTTACCACGCGGGCCCCTTtcaggcagag                                        >  3:72210/1‑138 (MQ=255)

                                                                                             caaaaccaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGcc                                      <  1:225602/140‑1 (MQ=255)

                                                                                                aaccaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTGTTTTTCGTGACGGCATCGACCACTCGCAGATAATGGGTT‑ATGATTTACCGCGCATGTTAGTAACAACCCTGGCCCCTTTCAGTAAGCGccccc                                   >  5:936079/1‑137 (MQ=255)

                                                                                                  ccaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCAcgcc                                 <  2:326392/140‑1 (MQ=255)

                                                                                                    aaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTGTGATTTGCGTTACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTGCGTAACAACGCTGGCCACTTTCAGTCAGCGCCAcgccgc                               <  5:482037/140‑1 (MQ=255)

                                                                                                       acTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgag                            <  3:323661/140‑1 (MQ=255)

                                                                                                       acTGAAACCAATGCGGATCATCCCCTCCGGGCTCTTTTTGATTTGCGTGAAGTCATCGACCATTCTCTGATCTTGGGGG‑AGGATTTACAGCTCATGTTCGTAACAACGCTGGATACTTTCAGTCATCGACACGCCGCgag                            <  6:217413/140‑1 (MQ=255)

                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTGAGCGCCACGCCGCgagcggag                       <  2:715854/140‑1 (MQ=255)

                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                       <  8:848545/140‑1 (MQ=255)

                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                       <  4:854710/140‑1 (MQ=255)

                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                       <  8:662792/140‑1 (MQ=255)

                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                       <  5:789565/140‑1 (MQ=255)

                                                                                                            aaCCAATGCGGAGCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                       <  1:157689/140‑1 (MQ=255)

                                                                                                            aaCCAAGGCTGATCATCCCCTCCGGGCGCGGTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGGTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                       <  5:176688/140‑1 (MQ=255)

                                                                                                               cAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGagcggagcgg                    <  6:13613/140‑1 (MQ=255)

                                                                                                                 aTGCGGATAATCCCCTCGGGGCGCGGTTTGATTTGCGTGAGGTCATCGACCAGTCGCTGATATTGGGTT‑AGGATTTCCAGCGCATGTTCTTAACATCGCTGGCCTCTTTCTGTGAGCGCCACGCGGCGAGCGGAGCGGtt                  <  7:745819/140‑1 (MQ=255)

                                                                                                                  tGCGGATCATCCCCTCCGGGCGCGTGTTGATTTGCGTGACGTCATAGACCCGTCGCGGATATTGGGTG‑TGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACATTAAGTCAGCGCCACCCCGCGagcggggcgggtg                 >  6:344421/1‑137 (MQ=255)

                                                                                                                    cGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcag               <  6:465903/140‑1 (MQ=255)

                                                                                                                      gATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATTTTGGGTG‑ATGATTTCCAGCGCATGTGCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCTGAGCGGTTcagca             <  1:360145/140‑1 (MQ=255)

                                                                                                                      gATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGTTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcagca             <  4:1021923/140‑1 (MQ=255)

                                                                                                                      gATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcagca             <  3:612958/140‑1 (MQ=255)

                                                                                                                      gATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcagca             <  1:396191/140‑1 (MQ=255)

                                                                                                                      gATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGCTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGTAGCGGTTcagca             <  7:40123/140‑1 (MQ=255)

                                                                                                                        tcatcCCCTCCGGGCGCGTTTTGTGTTGCGTGAAGTCATCGACCCGTGCCGGATATTGGGTG‑TGGGTTTGCCACGCCTGTTCGTAACAAACCTGGGCCAGTTCAGTCAGCGCCCCCCCGCCCGCGGGGCGGTGtagcagc           >  4:637717/1‑139 (MQ=255)

                                                                                                                         catcCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGGCGCGGATATTGGGTG‑AGGATTTGCCGCGCATGTTCGTAACAACGCTGGGCACATTCAGTCAGCGCCACCCCGCGAGCGGCGCGGTTCAGCagag          >  6:171000/1‑140 (MQ=255)

                                                                                                                         catcCCCTCCGGGCGCGGTTTGATTTTCGTGAAGTAATAGAACAGTCGCGGATATTGGGTG‑AGGATTTACAGCGCATGTTAGTAACAACCCTGGCCACATTAAATCATCCACACCCCGCGAGCGGAGCGGTTCAGCcgag          >  2:969370/1‑140 (MQ=255)

                                                                                                                                 ccGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGcc  <  1:640215/140‑1 (MQ=255)

                                                                                                                                 ccGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGcc  <  4:189758/140‑1 (MQ=255)

                                                                                                                                 ccGGGCGCGGTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTTGGTG‑AGGATTTACAGCGCATGTTCGTAACAACGCTGGCCCCATTAAGTCAGCGCCACGCCCCGAGCGGAGCGGTTCAGCAGAGTTGTTGcc  >  2:717115/1‑140 (MQ=255)

                                                                                                                                 ccGGGCGCGGTTTGAGTTGCGTGGCGTCATCGCCCAGTCGCGGATATTGGGTG‑GGGGTTTGCAGCGCCTGTTGGTAACAACGCGGGGCCCGTTCAGGCAGCGCCCCGCCGCGAGCGGAGCGGTTGAGGAGCGTTGTTGcc  >  8:425707/1‑140 (MQ=255)

AATCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGTTTTGATTTGCGTGACGTCATCGACCAGTCGCTGATATTGGGTG‑AGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCC  >  NZ_CP022686/1954785‑1955053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A