Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F36 I0 R1 275 10897.2 10515506 98.4% 10347257 135.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,954,923 T→G 19.2% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,954,9230TG19.2% 338.3 / 16.6 208T92P (ACG→CCG) ttdRDNA‑binding transcriptional activator TtdR
Reads supporting (aligned to +/- strand):  ref base T (46/116);  new base G (40/6);  total (86/122)
Fisher's exact test for biased strand distribution p-value = 9.16e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

AATCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCG  >  NZ_CP022686/1954785‑1955057
                                                                                                                                           |                                                                                                                                       
aaTCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑Gtt                                                                                                                                        >  6:363718/1‑140 (MQ=255)
aaTCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑Gtt                                                                                                                                        >  5:668103/1‑140 (MQ=255)
aaTCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑Gtt                                                                                                                                        >  7:1029173/1‑140 (MQ=255)
aaTCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑Gtt                                                                                                                                        >  6:748049/1‑140 (MQ=255)
    aaTTTGGAGAGCGAACAATTCAAAATGCACCTGTAACTCACGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGa                                                                                                                                    <  8:1016326/140‑1 (MQ=255)
     aTTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGAt                                                                                                                                   >  6:671281/1‑140 (MQ=255)
       ttGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTGTTGAt                                                                                                                                   >  2:758277/1‑138 (MQ=255)
       ttGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGAt                                                                                                                                   >  3:480562/1‑138 (MQ=255)
            gatcgaACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCg                                                                                                                              >  3:587522/1‑138 (MQ=255)
             atcgcACAATTCAAAATGGACCTGTAACTGAGGATAAATGCGCATCAGTTCGGTAAAAGCTGGCGCAATATGGCTGCGCCCAAAAACAAAACTGCAACCAATTCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑a                                                                                                                          <  7:232486/140‑1 (MQ=255)
                       tCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGAc                                                                                                                <  7:349618/140‑1 (MQ=255)
                         aaaaTGCACCTGTAACTCAGGATCATTGCGCATCAGTGCGGTAAGAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑CCGTCATCGACCa                                                                                                              <  6:908093/140‑1 (MQ=255)
                         aaaaTGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGAc                                                                                                                <  4:336833/138‑1 (MQ=255)
                           aaTGCACCTGTATCTCAGGATAATTGCGCATCATTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCa                                                                                                              <  1:149155/138‑1 (MQ=255)
                           aaTGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGt                                                                                                            <  5:831686/140‑1 (MQ=255)
                           aaTGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGt                                                                                                            <  7:477908/140‑1 (MQ=255)
                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGt                                                                                                            <  4:1193020/138‑1 (MQ=255)
                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGt                                                                                                            <  1:270134/138‑1 (MQ=255)
                             tGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGATTTTCGGG‑ACGTCATCGACCAgg                                                                                                            >  3:937890/1‑137 (MQ=255)
                               cACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCACCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCt                                                                                                        <  5:173991/140‑1 (MQ=255)
                                aCCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGATTTGCGTG‑ACGTCATCGACCAGTCGcgg                                                                                                       >  5:911803/1‑138 (MQ=255)
                                   tGTAACTCAGGATAATTGCGCATCAGTTCGGTACTAGCTGGCGCAATATGGCTGCGCCCAAAACAAAAACTGCAACCAATGCGGAGCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGa                                                                                                      <  1:754855/138‑1 (MQ=255)
                                       aCTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCAACGCCCAGGCGGTGATATTgg                                                                                                >  8:1136426/1‑140 (MQ=255)
                                          cAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTggg                                                                                               <  1:722579/138‑1 (MQ=255)
                                          cAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTg                                                                                             <  8:983595/140‑1 (MQ=255)
                                            ggATATTTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAg                                                                                           <  8:833060/140‑1 (MQ=255)
                                            ggATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTTATTTGGGTG‑ACGTAATAGAACAGTCGCGGATATTTGTTGAg                                                                                           >  6:1192516/1‑140 (MQ=255)
                                            ggATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCGGATATTTGGTGAg                                                                                           >  7:316555/1‑140 (MQ=255)
                                              aTAATTGCGCATCAGTTCGGTACTAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTGTGATTTGCGTG‑ACGGCATCGAACAGTCGCGGATATTgggggtg                                                                                           >  4:957976/1‑134 (MQ=255)
                                                     cgcATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTcccg                                                                                  >  6:587139/1‑138 (MQ=255)
                                                       cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGCGGTTTTGTTTTG‑GTG‑GCGTCATAGCCCAGTCGCGGATATTTGGTGTGGAtttaaaccg                                                                                >  5:533924/1‑134 (MQ=255)
                                                       cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTAATCGCCCAGTCGCGGATATTGGGTGTGGATTTCCAg                                                                                  >  4:58952/1‑138 (MQ=255)
                                                       cATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGATTTGCGTT‑GCGTCATCAACCCGTCGCTGATTATTGGTGAGGAATTCCcgcg                                                                                >  8:1250425/1‑140 (MQ=255)
                                                        aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAgcgc                                                                               <  5:1218354/140‑1 (MQ=255)
                                                        aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAgc                                                                                 <  2:716599/138‑1 (MQ=255)
                                                        aTCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAgc                                                                                 <  4:999679/138‑1 (MQ=255)
                                                         tCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑CCGGAATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAgcg                                                                                >  1:665237/1‑138 (MQ=255)
                                                             ttCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATg                                                                            <  4:181249/138‑1 (MQ=255)
                                                             ttCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATg                                                                            <  4:537870/138‑1 (MQ=255)
                                                             ttCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATg                                                                            <  4:687096/138‑1 (MQ=255)
                                                             ttCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGtt                                                                          <  5:412812/140‑1 (MQ=255)
                                                              tCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTTATTTGGGTG‑GCCTCATAGCCCAGTCGGGGTGATTTGGGGGGGTTTTTCAGCGCCTGt                                                                           >  4:1337085/1‑138 (MQ=255)
                                                                  tAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑TCGTCATCGACCAGTCGCTGATATTTGGTGAGGATTTACATCGCATTTTAGtta                                                                     >  7:866558/1‑138 (MQ=255)
                                                                       gCTGGCGCAATATGGCTGCGCCCAACACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGGTTGATTTGCGTGCACG‑CAGCGCCCAGTCGCCGCTTTTGGGTGTGTCTTTGCAGCGCATTGTCGTaacacac                                                                >  6:1019503/1‑137 (MQ=255)
                                                                       gCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGG‑GTTTTGATTTGCGTG‑ACGTCATCGCCCAGTCGGGGATATTGTGTGAGGTTTTCCAGCGCATGTTAGTAACAACg                                                                >  8:624612/1‑140 (MQ=255)
                                                                       gCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTaacaa                                                                  <  3:14593/138‑1 (MQ=255)
                                                                       gCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTTAATTGCGTG‑ACCTCATCGCCCAGGCGCGGGTATTGGGGGAGGATTTACCGCGCATGTTCGGAAAAACg                                                                >  6:1152815/1‑140 (MQ=255)
                                                                         tGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGATTTGCGTG‑GCCTCATCGCCCAGTCGCTGATATTGGGTGAGGATTTCCACCGCCTGTTCGTAACAACg                                                                >  2:712066/1‑138 (MQ=255)
                                                                         tGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGGTTGATTTGGGTG‑CCGTCCTCGCCCACTCGCGGATATTGGGTGGGGGTTTAAAGCGCATGTTGGTaacaacc                                                                >  3:1158830/1‑137 (MQ=255)
                                                                          ggCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGc                                                               <  3:17794/138‑1 (MQ=255)
                                                                           gcgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGGGTG‑ACGGCATCGACCAGTCGCTGATTTTGGGTGGGGGTTTCCAGCGCATGTTGGTAACAACCCTgg                                                            >  7:592438/1‑140 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCTAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                           <  8:903838/140‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCTATGCGGATCATCCCCTCCGGGAGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGGCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                           <  7:1165756/140‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTg                                                             <  4:911833/138‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTg                                                             <  2:1352136/138‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTg                                                             <  4:1042487/138‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTg                                                             <  3:707676/138‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                           <  6:695710/140‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                           <  8:481291/140‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                           <  5:277580/140‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                           <  6:139570/140‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                           <  5:95055/140‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                           <  5:709642/140‑1 (MQ=255)
                                                                            cgcAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTGTTTATTTGCGTG‑ACGTCATCGACCACGAGCTGATATTTGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGc                                                           >  7:946474/1‑140 (MQ=255)
                                                                              cAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑CCGTAATCGCCCAGTCGCTGATTTTTGGTGAGGATTTACAGCGCTTGTTCGTAACAACGCgggg                                                           >  2:109805/1‑137 (MQ=255)
                                                                              cAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTggg                                                           >  2:625918/1‑137 (MQ=255)
                                                                                 tatGGCTGCGCCCAAAACCAAAACTGCCACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTTCGTG‑ACGTCATCGACCAGGCGCGGATATTGGGTGTGGGTTTCCCGCGCATGTTCGTAACAACGCTGGccccat                                                      >  6:534097/1‑136 (MQ=255)
                                                                                 tatGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACtt                                                      <  5:1106722/140‑1 (MQ=255)
                                                                                 tatGGATGCGCCCAAAACCAACAATCCAACCAATGAGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGGG‑ACGTCATCGACCAGTCGCTGATATTGGGCGCGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACtt                                                      <  7:803780/140‑1 (MQ=255)
                                                                                  atGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCGGATATTGGGGGAGGATTTACAGCGCATGTTCGTAAAAACCCTGGGCCCttt                                                     >  8:739617/1‑140 (MQ=255)
                                                                                      cTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGTCGC‑GTTTTGATTTTCGGG‑ACGTCATAGACCCATCGCTGATAATGGGTGGTGATTTCCAGCGCAGGTTCGTAACAACGCTGGCCACATtaagt                                                 >  8:79472/1‑140 (MQ=255)
                                                                                        gcgcCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTtcagt                                                 <  3:513290/138‑1 (MQ=255)
                                                                                           cccAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTTCGTG‑TCGTCATAGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTtacgtcag                                              >  2:860579/1‑138 (MQ=255)
                                                                                           cccAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGGGTG‑ACATAATCGACCAGTCGGTGATATTTGGTTTGGATATGCAAGCCATGGTCGAAAAAACGCAGGCCACTTACAGTAAgcg                                            >  6:394666/1‑140 (MQ=255)
                                                                                           cccAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑TCGTCATCGACCAGTCGCTGATATTGGGTGAGGCTTTCCAGCGCATGTTAGTAACAACGCTGGCCACTTtaagtcag                                              >  3:728384/1‑138 (MQ=255)
                                                                                            ccaaaaccaaaaCTGCAACCAATTCTGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGGCATCGACCAGGCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACTCTGGCCACTTTCAGTCAgcgc                                           <  8:310922/140‑1 (MQ=255)
                                                                                             caaaaccaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTTGGTGAGGATTTACAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAgcg                                            >  4:279206/1‑138 (MQ=255)
                                                                                             caaaaccaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGcc                                          <  6:1103799/140‑1 (MQ=255)
                                                                                             caaaaccaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGTTTTGGGTG‑ACGTCATCGACCCGTGGCTGTTTTTGGGTGGGGTTTTGCCGCGGCTGTTCGTTACAACCCGGGGCCCTTTGAGTCAgcc                                            >  4:386514/1‑137 (MQ=255)
                                                                                             caaaaccaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGcc                                          <  7:514780/140‑1 (MQ=255)
                                                                                             caaaaccaaaacTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GGTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGGTCGTAACAACGCTGGCCACTTTCAGTCAgcg                                            >  4:1282406/1‑138 (MQ=255)
                                                                                                            aaCCTATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACGAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                             <  4:806272/138‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                           <  6:94895/140‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                           <  6:162858/140‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                           <  7:214842/140‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                             <  3:131016/138‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                             <  3:389229/138‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                             <  2:346116/138‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                             <  2:150181/138‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                             <  1:1195670/138‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTGCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                             <  2:1188569/138‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGGCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcgg                             <  1:712066/138‑1 (MQ=255)
                                                                                                            aaCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGAATTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCgagcggag                           <  8:1208490/140‑1 (MQ=255)
                                                                                                             aCCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGGGTG‑ACGTGATCGACCAGTCGCTGATATTGGGTGAGGATTTGCAGCGCATGTTCGTTACAAAGCTGGCCACGTTCAGTCAGCGCCCCGCCCCgagcggg                            >  2:479320/1‑137 (MQ=255)
                                                                                                                  tGCGGATCATCCCCTCCGGGCGC‑GGTTTGATTTGCGTG‑ACGTCATAGCCCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCAATTTCGTAACAACCCTGGCCACTTTCCGTCAGCGCCACGCCGCGCGCGGCGCGGttg                     >  6:964354/1‑139 (MQ=255)
                                                                                                                      gctcatcCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCAGCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGGCAGCGCCACGCCGCGAGCGGAGCGGTTcag                   <  1:1281086/136‑1 (MQ=255)
                                                                                                                      gATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcagca                 <  8:290054/140‑1 (MQ=255)
                                                                                                                      gATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTcagca                 <  5:145342/140‑1 (MQ=255)
                                                                                                                                ttcgtgcgc‑GGTTTGATTTGCGTG‑ACGTCATAGACAAGTCGATTATATTTGTTGATGCTTTACAGATAATGTGAGTAACAACGCTGGACACTTGAAGTAATCTCCACGCCTCGAGCGTAGCGGTTCAGAAGAGTTGTTGc       <  6:1076097/135‑1 (MQ=255)
                                                                                                                                    ggCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCa     <  1:43827/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
AATCAATTTGTCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATGCGGATCATCCCCTCCGGGCGC‑GTTTTGATTTGCGTG‑ACGTCATCGACCAGTCGCTGATATTGGGTGAGGATTTCCAGCGCATGTTCGTAACAACGCTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGAGTTGTTGCCAGCG  >  NZ_CP022686/1954785‑1955057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A