Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F12 I0 R1 131 9881.0 7547414 98.3% 7419107 134.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,632,501 T→G 9.7% V166G (GTT→GGT)  arcC → carbamate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,632,5010TG 9.7% 295.2 / 6.7 168V166G (GTT→GGT) arcCcarbamate kinase
Reads supporting (aligned to +/- strand):  ref base T (41/105);  new base G (15/5);  total (58/110)
Fisher's exact test for biased strand distribution p-value = 7.15e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

GGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATA  >  NZ_CP022686/1632364‑1632632
                                                                                                                                           |                                                                                                                                   
ggCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTAGTTGCTTCG‑CCCAAAC‑CGGt                                                                                                                                     >  6:818739/1‑138 (MQ=255)
  cATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTg                                                                                                                                   <  2:504662/138‑1 (MQ=255)
   aTTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCTTTGCTTCG‑CCAAAAC‑CGGTTGa                                                                                                                                  >  2:675792/1‑138 (MQ=255)
       cTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAACACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTAGG‑CCCAAAC‑CCGGTGATATCAt                                                                                                                            >  3:782767/1‑140 (MQ=255)
            cccGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTAG‑CCAAAAC‑CGGTTGATATCATTGaaa                                                                                                                       >  4:710714/1‑140 (MQ=255)
                aCCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTAG‑CCAAAAC‑CGGTTGATATCAATGAAAAAGa                                                                                                                   >  4:496174/1‑140 (MQ=255)
                 ccAATCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTGCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAg                                                                                                                    <  2:339717/138‑1 (MQ=255)
                 ccAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAg                                                                                                                    <  1:211683/138‑1 (MQ=255)
                 ccAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGATGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGaa                                                                                                                  >  4:741640/1‑140 (MQ=255)
                 ccAAGCCGATCGGTTCGTGCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGaa                                                                                                                  <  4:868024/140‑1 (MQ=255)
                 ccAAGACGATCGGTTCGTTCTTAACCGAGCAAGAAGCAGAGCTTCTGACTAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGaaaaac                                                                                                                    <  2:739942/138‑2 (MQ=255)
                  cAAGCCGATCGGTTCGTTCTTTCCCGCGCAAGAAGCGGAGCTCCGACCAAAACCGGGTACCCCGCTGAACGAAGATGCGGGGCCCGGCTTTCTGCGTGGGGTTGCTTCG‑CCAAAAC‑CGGTGGATTAAATTTaaaaaaa                                                                                                                   >  6:342113/1‑136 (MQ=255)
                  cAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTAG‑CCAAAAC‑CGGTTGATATAATTTAAAAAGa                                                                                                                   >  1:332580/1‑138 (MQ=255)
                  cAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTTGTTAG‑GCAAAACACGGTT‑ATATAATTTaaaaaaa                                                                                                                   >  5:809757/1‑136 (MQ=255)
                  cAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGa                                                                                                                   >  5:543975/1‑138 (MQ=255)
                        gATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGt                                                                                                             <  1:49479/138‑1 (MQ=255)
                         aTCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGtt                                                                                                            <  5:249332/138‑1 (MQ=255)
                          tCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTaaa                                                                                                         >  3:756850/1‑140 (MQ=255)
                           cGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTCG‑CCAAAAC‑CGGGTGATATCATTGAAAAAGAAACGGTTaa                                                                                                          >  7:189455/1‑138 (MQ=255)
                           cGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTaa                                                                                                          <  7:357641/138‑1 (MQ=255)
                             gttggtttTTTACTGAGAAAGAAGAATAGATACTGCATAAACAGGTTTACACTGTGAAAGATGATGGGGGTCGCGGGTATGGTCTGGTCGTTGCTTGG‑CCAAAAC‑CGGTTGATTTCATTTAAAAAGAAACGGTTAAAGct                                                                                                      <  4:251634/140‑1 (MQ=255)
                                  ttcTTAACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTg                                                                                                   <  1:614768/138‑1 (MQ=255)
                                       tACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGa                                                                                              <  6:543975/138‑1 (MQ=255)
                                        aCCGCGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCAGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGAt                                                                                             <  7:131359/138‑1 (MQ=255)
                                        aCCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAATCGGTTAAAGCTCTGGTAGAt                                                                                             <  7:404024/138‑1 (MQ=255)
                                        aCCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGAt                                                                                             <  7:644785/138‑1 (MQ=255)
                                        aCCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGAt                                                                                             <  1:540178/138‑1 (MQ=255)
                                         ccGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CCGTTGAAATCAATTAAAAAGAAACGGTTAAAGCTCTGGTataat                                                                                            >  8:404024/1‑134 (MQ=255)
                                          cGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGc                                                                                           <  8:185651/138‑1 (MQ=255)
                                          cGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTGGGTGCTTCGCCCAAAAC‑CGG‑TGATATCATTGTAAAACAAAACGGTAAAGCGCAGGGAGATGc                                                                                           >  1:159726/1‑138 (MQ=255)
                                             gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCgg                                                                                        <  7:143746/138‑1 (MQ=255)
                                             gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGAc                                                                                      <  4:756850/140‑1 (MQ=255)
                                              cAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTCG‑CCAAAAA‑CGGGTGGTATCATTGAAAAATAAACGGTTAAAGCTCTGGGTGATGCCggg                                                                                       >  1:349883/1‑137 (MQ=255)
                                                   agcagcGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCGTCC‑GCAAAAC‑CGGGTGCTATCATTGAAAAAGAAACGGTTAAAGGAGTGGTAGAATCCgggccggg                                                                                  >  7:34206/1‑132 (MQ=255)
                                                       tagCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGAt                                                                            <  4:536217/139‑1 (MQ=255)
                                                        agCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGa                                                                             <  5:9237/138‑1 (MQ=255)
                                                         gCTACTGACAAAACAGGGTGACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGGTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAATCTCTGGCAGATGCCGGACAGGTGGTGAt                                                                            <  7:780715/138‑1 (MQ=255)
                                                          cTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGGAGCTGCCGGACAGGTGGTGAta                                                                           >  7:300678/1‑137 (MQ=255)
                                                          cTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCgg                                                                                        <  5:857621/125‑1 (MQ=255)
                                                              tGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGCGATCACCGtt                                                                     <  3:469118/140‑1 (MQ=255)
                                                                aCAAAACAGGGTTACAGTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGAGCACCGtt                                                                     <  2:368554/138‑1 (MQ=255)
                                                                aCAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTgg                                                                   <  3:898239/140‑1 (MQ=255)
                                                                   aaaCAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGGTGCATCG‑CCAAAAC‑CGTTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCgg                                                                <  3:717908/140‑1 (MQ=255)
                                                                    aaCAGGGTTACACTCTGAAAGAAGATGCCGGTCGAGGCTATCGTCGTGTACTTGCTTGT‑CCAAAAA‑CCGTTGATAAAATTGAAAAAGAAAAGTGTAAAGCAAAGGTAAATGCCGGCCAAGAGGTGAAAACCGTTTGCg                                                                 >  8:700575/1‑138 (MQ=255)
                                                                      cAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggt                                                               <  2:137742/138‑1 (MQ=255)
                                                                         ggTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggt                                                            <  2:677240/138‑1 (MQ=255)
                                                                         ggTTACAATCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAATAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggt                                                            <  7:80607/138‑1 (MQ=255)
                                                                           ttACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTTCTTCG‑CCAAAAC‑CCGTTGATATCATTGAAAAAGAAAAGGTTAAATCTCTGGTAGATTCCGGACAGGTGGGGATAACCGGTGGCggtggtgg                                                          >  2:963433/1‑138 (MQ=255)
                                                                           ttACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggtgg                                                          <  1:351147/138‑1 (MQ=255)
                                                                            tACACTCTGAACGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGACACGGTTAACGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTAt                                                       <  3:182270/140‑1 (MQ=255)
                                                                            tACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAACCGGTTAAAGCTCTGGTAGATGCCGGACAGGGGGTGAGCACCGTTGGCggtggtggt                                                         <  5:67786/138‑1 (MQ=255)
                                                                            tACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAA‑CCGGTGATATCATTTAAAAAGAAAAGGGTAAAGCGCTGGTAGATGACGGACAGGTGGTGGTACCCGTTGGCgggggtggt                                                         >  6:807595/1‑138 (MQ=255)
                                                                            tACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCGTAG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggtggt                                                         >  1:7992/1‑138 (MQ=255)
                                                                               acTCTGAATGACGATGCGGGGCGAGGGTAGCGTCGTGTCGTTGCTGCG‑CCAATAG‑CGGTTGTTATCATTGAAAAAGAAACGGCTAAAGCGCTTGTAGATGCCGGACAGGTGGTGATCACAGTTGGCGGTGGTGGTATc                                                      <  2:105432/138‑1 (MQ=255)
                                                                                ctctGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACCGGTGGGGCTCACCGGTGGCggtggtgggttcc                                                     >  2:497492/1‑133 (MQ=255)
                                                                                ctctGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTGCTTCG‑CCAAAAC‑CGGTTGATATAAATGAAAAAGAAACGGGTTAAGCGCTGGTTGATGGCGGGCAGGTGGTGATCACCGTTGGCGGTGGTGGTATcc                                                     >  8:786993/1‑138 (MQ=255)
                                                                                  ctGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCt                                                   <  6:849076/138‑1 (MQ=255)
                                                                                   tGAAAGAAGTAGCTGGTCTCGGCTATCGTCGTGGCGTTGCTTCG‑CCAAAAC‑AGGTTGATATCATTGAAAAATAAACGGTTAAATCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTg                                                  <  8:587952/138‑1 (MQ=255)
                                                                                   tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTCG‑CCAAAAC‑CGGTTGATATAATTGTAAAATAAACGGTTAAAGCTATGGTTGATGCCGGACAGGTGGTGATCACCCGTGGGGGGGGTGGTATACCTGtt                                                >  3:122245/1‑140 (MQ=255)
                                                                                   tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTTAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCCTTGGCGGTGGTGGTATACCTGtt                                                >  4:956109/1‑140 (MQ=255)
                                                                                   tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGGTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCCGACAGGTGGTGATCACCGTTGGCGGTGGTGGGATACCTGtt                                                >  4:310390/1‑140 (MQ=255)
                                                                                   tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGATTCG‑CCAAAAC‑CGGGTGATATCATTGAAAAAGAAACGGTTAAATCTCTGGTAGATGCCGGGCAGGTGGTGATCACCGTTTGCGGTGGTGGTTTCACTggt                                                >  3:533708/1‑138 (MQ=255)
                                                                                     aaaGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTTCTTCG‑CCAAAAC‑CCGGTGATATCATTTAAAAAGAACCGGTTAAAGCGCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATACCAGtt                                                >  8:366741/1‑138 (MQ=255)
                                                                                     aaaGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGCTATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGCTTCCCGAAAGGTTGTGATCACCGTTGGCGGTTGTGGTATCCCAGtt                                                >  8:587648/1‑138 (MQ=255)
                                                                                     aaaGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTTAAGGTCTGGTATGTTCCGGACAGGTGGTGATCCCCGTTGGCGGTGGTGGTATACCTGtt                                                >  8:326450/1‑138 (MQ=255)
                                                                                     aaaGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTGCTTCG‑CCAAAAC‑CCGTTGATATAATTGAAAAAGAAACGGTTAAAGCTATGGTTGATGCCGGACAGGGGGGGATCACCGTTGGCGGGGGTGGTATACCGGtt                                                >  6:843521/1‑138 (MQ=255)
                                                                                        taagaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTATAGCTCTGGTTGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTAtt                                             <  5:202347/137‑1 (MQ=255)
                                                                                        gaagaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTAtt                                             <  8:841704/138‑1 (MQ=255)
                                                                                         aagaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGt                                          <  3:266262/140‑1 (MQ=255)
                                                                                         aagaTGCGGGTCGAGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑AGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTc                                            <  7:860910/138‑1 (MQ=255)
                                                                                           gaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCTTTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGt                                          <  6:363245/138‑1 (MQ=255)
                                                                                           gaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGt                                          <  8:17477/138‑1 (MQ=255)
                                                                                           gaTGCGGGTCGAGGCTATCTTCGTGTCGGTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACTGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGa                                        <  3:162407/140‑1 (MQ=255)
                                                                                           gaTGCGGGGCGCGGCTATCGGCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGt                                          <  6:159492/138‑1 (MQ=255)
                                                                                           caTGCGGGGAGCGGCTATCGTCGTGTCGTGTCTTCG‑CCAAAAC‑CTGGTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTTGGATCCCTGTTATTCGt                                          <  5:401333/137‑1 (MQ=255)
                                                                                                     gcgGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTTAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTTGTGGTATCCCTGTTATTCGTGAAGGTAAcc                                <  2:831397/138‑1 (MQ=255)
                                                                                                      cgGCTATCGTCGTGTCGGTTCGTCG‑CCCAAAC‑CGGGTGGTATCATTGAAAAAGAAACGGGTAAAGCTGTGGTAGATGCCGGACAGGTGGGGCTCAACGTTGGCGGTGGTGGGTTCCCTGTTTTTCGTGAAGGTAACCATc                             >  4:14734/1‑140 (MQ=255)
                                                                                                          tATCGTCGTGTCGTTGCTTCT‑CCAAAAC‑CGGTTGATATCATTTAAAAAGAAACGGTTAAAGCTCTGGGAGATGCCGGACAGGGGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTg                           <  6:766231/138‑1 (MQ=255)
                                                                                                          tATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACCGGTGGTGATCACCGTTGGCGGTGGTGGTAGCCCTGTTATTCGTGAAGGTAACCATCTGct                         <  4:431639/140‑2 (MQ=255)
                                                                                                          tATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTg                           <  5:909327/138‑1 (MQ=255)
                                                                                                          tATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTg                           <  2:613489/138‑1 (MQ=255)
                                                                                                          tATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTg                           <  6:546125/138‑1 (MQ=255)
                                                                                                          tATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCg                         <  4:208781/140‑1 (MQ=255)
                                                                                                          tATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATACGTGAAGGTAACCATCTg                           <  8:722082/138‑1 (MQ=255)
                                                                                                          tATCGTCGTGTCGTTGCTTCC‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACTGTTAAAGCTCTGGTAGATGCCGGTCAGGTGGTGATCACCGTTGGCTGTGGTGGTATCCCTGTTATTCGAGAAGGTAACCATCTg                           <  2:322018/138‑1 (MQ=255)
                                                                                                          tATCGTCGTGTCGTTGCGTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTg                           <  6:642587/138‑1 (MQ=255)
                                                                                                          tATCGTCGTGTCGGTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCg                         <  3:683607/140‑1 (MQ=255)
                                                                                                                     cgtTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAgcg              <  3:577568/140‑1 (MQ=255)
                                                                                                                     cgtTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAg                <  8:442782/138‑1 (MQ=255)
                                                                                                                      gtTGCTTCG‑CCAAAAC‑CGGTTGAGATCATTGAAAAAGAAACGGGTAAAGCTCTGGTAGATTCCGGACCTGTGGTGATAACCGGTGGCGGTGGGGGTAGCCCAGGTATTAGTGGAAGTTACCATCTTCGTGGTGCCAgc               >  2:85290/1‑138 (MQ=255)
                                                                                                                           ttCG‑CCAAAAC‑CTGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTa        <  4:411351/140‑1 (MQ=255)
                                                                                                                           ttCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGt          <  2:213289/138‑1 (MQ=255)
                                                                                                                           ttCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGt          <  6:446885/138‑1 (MQ=255)
                                                                                                                           gtCG‑ACAAAAC‑CGGTTGTTATCCTTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCAGGACAGGTGGTGAGCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTa        <  4:53897/139‑1 (MQ=255)
                                                                                                                            tCG‑CCAAAAC‑CGGTTGATATCATTGAAAAACAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGtt         <  1:757634/138‑1 (MQ=255)
                                                                                                                            tCG‑CCAAAAC‑AGGTTTATATCATTGAAAAAGTAAGGGTTAAAGCTCTGGAAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGtt         <  8:721817/138‑1 (MQ=255)
                                                                                                                                ccAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggt                                                               <  6:695987/81‑1 (MQ=255)
                                                                                                                                  aaaaC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGa    <  2:7992/138‑1 (MQ=255)
                                                                                                                                  aaaaC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGa    <  2:557573/138‑1 (MQ=255)
                                                                                                                                  aaaaC‑CGGGTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGa    <  6:883792/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                   
GGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCG‑CCAAAAC‑CGGTTGATATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATA  >  NZ_CP022686/1632364‑1632632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A