Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A27 F36 I0 R1 164 7919.5 7665810 98.4% 7543157 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,632,501 T→G 12.4% V166G (GTT→GGT)  arcC → carbamate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,632,5010TG12.4% 281.7 / 6.0 168V166G (GTT→GGT) arcCcarbamate kinase
Reads supporting (aligned to +/- strand):  ref base T (40/102);  new base G (21/4);  total (61/107)
Fisher's exact test for biased strand distribution p-value = 1.81e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

CCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTGG  >  NZ_CP022686/1632362‑1632640
                                                                                                                                            |                                                                                                                                            
ccGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGt                                                                                                                                              <  7:853147/140‑1 (MQ=255)
ccGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTGCTTCGCC‑AACACCGGt                                                                                                                                              >  6:496589/1‑140 (MQ=255)
ccGGCATACCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGGAGGTTCGCC‑AAAACCGGt                                                                                                                                              >  4:962753/1‑140 (MQ=255)
              cccGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGGTGGCC‑AAAACCGGGTGATATCATTGaa‑a                                                                                                                               >  3:291787/1‑140 (MQ=255)
                cGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTGGTTTCTTCGCC‑AAAAACGGTTTATATAATTGta‑aaa                                                                                                                             >  7:874237/1‑140 (MQ=255)
                  aCCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGa                                                                                                                           >  5:493065/1‑140 (MQ=255)
                  aCCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGGTGTTTTCATTGAA‑AAAGa                                                                                                                           >  5:556604/1‑140 (MQ=255)
                   ccAAGCCGATCGGTTCTTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGTGTTACACTCTGAAAGAAGATGCGTGTCGCGGCTATCGTCGTGGCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑ACAGaa                                                                                                                          <  3:463180/140‑1 (MQ=255)
                   ccAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGaa                                                                                                                          <  1:637864/140‑1 (MQ=255)
                   ccAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTAGCC‑AAAACCGGTTGATATCATTGAA‑AAAGaa                                                                                                                          >  2:73569/1‑140 (MQ=255)
                      aGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATAATTGAA‑AAAGaaaag                                                                                                                       >  1:314783/1‑138 (MQ=255)
                          gctCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTa                                                                                                                   <  3:730589/138‑1 (MQ=255)
                           ttctgttcgttcTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCTTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTaa                                                                                                                  <  4:853593/136‑1 (MQ=255)
                           aTCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGGTGATATCATTGAA‑AAAGAAACGGTTaa                                                                                                                  >  1:624523/1‑140 (MQ=255)
                           aTCGGTTCGTTCGTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACAATGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCCTTTCATAGCCAAAAACCGGTT‑ATAACAATTAT‑AAAAAAACGGTTaa                                                                                                                  >  4:844365/1‑140 (MQ=255)
                             cGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAg                                                                                                                <  2:659271/140‑1 (MQ=255)
                                    ttcTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTCGCC‑AAAACCGGGTGCTATCATTGTT‑AAAGAAACGGTTAAAGCTCTGGt                                                                                                         >  1:693826/1‑140 (MQ=255)
                                    ttcTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGt                                                                                                         <  1:519792/140‑1 (MQ=255)
                                    ttcTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGt                                                                                                         <  8:694723/140‑1 (MQ=255)
                                    ttcTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACAGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGt                                                                                                         <  8:775703/140‑1 (MQ=255)
                                    tgcTTTACCGCGCAAGCAGAAGAGCTACTGTCAAACCAGGGTTACACTCTGAATGAAGATGCGGGTCGCGGCTAGCGTCGTGTCGTTGCTTCGCC‑AAAAGCGGTTGATATCATTGAA‑ATAGCAACGGTTAAAGCTCTGGt                                                                                                         <  4:684676/138‑1 (MQ=255)
                                         tACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTTCTTCGCC‑AAAACCGGTTGATATAAATGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATg                                                                                                    >  4:893042/1‑140 (MQ=255)
                                          cccGAGCATTAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGc                                                                                                   <  1:924752/139‑1 (MQ=255)
                                          cccGACCAAGAAGCAGCGCTACTTACAAAACAGGGGTACACTCTGACAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAT‑AAAGAAACGGTTAAAGCTCTGGTAGATGc                                                                                                   <  5:655833/139‑1 (MQ=255)
                                          aCCGAGCACGAAGCATAGCTACTGACAAAAAAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGc                                                                                                   <  7:825654/140‑1 (MQ=255)
                                            cGAGCAAGACGCAGAGCTACTGACAAAACAGGGTTACACTCTGTAAGTAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGATTCGAC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCg                                                                                                 <  3:98271/140‑1 (MQ=255)
                                             gAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCATCGCC‑CAAACCCGGTGAGATCATTTAA‑AAAGAAACGGGTAAAGCGAGGGTAGATGcccg                                                                                                >  6:115957/1‑138 (MQ=255)
                                              aGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTGGTTTGTTGGCC‑AAAACCGGGTTATTTCATTGAA‑AAAGAACAGGTTAAAGGATGGGTAGTTGCCggc                                                                                               >  1:886917/1‑139 (MQ=255)
                                              aGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGGTCGCC‑CAAACCCGTTGATATCATTGAA‑AAAGAAAACGTTAAATCTCTGGGAGTTGGCggg                                                                                               >  3:437396/1‑139 (MQ=255)
                                              aGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCggc                                                                                               >  2:532996/1‑139 (MQ=255)
                                              aGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGa                                                                                               <  2:285014/140‑1 (MQ=255)
                                              aGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGACGGa                                                                                               >  8:642882/1‑140 (MQ=255)
                                              aGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAAAAAAGGTTAAAGCTCTGGTAGATGCCGGa                                                                                               >  7:540085/1‑140 (MQ=255)
                                              aGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGGTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGa                                                                                               >  6:54387/1‑140 (MQ=255)
                                              aGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGGGGTGCTTGGCC‑CAAACCCGGTGATATCATTTTA‑AAACAAACCGTTAAAGCACTGGTTGAttgcggg                                                                                               >  1:543594/1‑134 (MQ=255)
                                               gCAAGAAGGAGATCTACTGACAATACAGGGTTACACTCTGAAAGAAGATGCGGGGCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGAc                                                                                              <  7:613867/140‑1 (MQ=255)
                                               gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTGGGTGGTTGGCC‑CAAACCGGTTGGTATAATTGAATAAAGAAA‑GGTTTAATCTCAGGTTGATGGCggcc                                                                                              >  7:617814/1‑138 (MQ=255)
                                               gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGAc                                                                                              <  6:539971/140‑1 (MQ=255)
                                               gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGAc                                                                                              <  7:1131/140‑1 (MQ=255)
                                                 cagcagCAGAGCTACTGACAAAACTGGGTTACACTCTGAAATCAGATGCGGGGCGCTGCTATCGTCGTGTCGTTGCTTCTCC‑AAAACCGGGTGATATCATTGAA‑AAAGAAACGGTTAAAGCGCTGGTAGATGCCGGACAg                                                                                            <  6:169344/136‑1 (MQ=255)
                                                        agagCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGGC‑AAAACCGGTTGATTTAATTGTA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGa                                                                                     >  8:690604/1‑140 (MQ=255)
                                                         cagCTACTGACAAAACAGGGTTACACTCTTAAAGAAGATGCGGGTCGCGGCTATCGGCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGAt                                                                                    <  6:637167/139‑1 (MQ=255)
                                                          agCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTGGCC‑AAAAACGGTTGTTATCATTGAA‑AAAGCAACGGGTAAAGCTATGGGAGATGCCGGACAGGTGGTGAta                                                                                   >  5:871035/1‑139 (MQ=255)
                                                          agCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATc                                                                                   <  7:365407/140‑1 (MQ=255)
                                                          agCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCAATGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATc                                                                                   <  6:122754/140‑1 (MQ=255)
                                                              aCTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGGTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCGGTGGTAGATGCCGGCCAGGTGGTGATCACCg                                                                               >  6:808665/1‑140 (MQ=255)
                                                                    aaaaCAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCg                                                                         <  1:526422/140‑1 (MQ=255)
                                                                    aaaaCAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCg                                                                         <  5:748948/140‑1 (MQ=255)
                                                                    aaaaCAGGGTTACACTCTGAAAGAAGATGCGGGGCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCg                                                                         <  2:726910/140‑1 (MQ=255)
                                                                     aaaCAGGGTTACACTGAGAAAGTAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCgg                                                                        <  2:886917/140‑1 (MQ=255)
                                                                     aaaCAGGGTTACACTCTTAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTTCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCTGACAGGTGGTGATCACCGTTGGCgg                                                                        <  2:848633/140‑1 (MQ=255)
                                                                     aaaCAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCgg                                                                        <  4:29442/140‑1 (MQ=255)
                                                                         aGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAAACCGGTGATATAATTTAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggt                                                                    >  3:263887/1‑140 (MQ=255)
                                                                           ggTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggtgg                                                                  <  5:122461/140‑1 (MQ=255)
                                                                           ggTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggtgg                                                                  <  4:136361/140‑1 (MQ=255)
                                                                            gTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCGTAGCC‑AAAACCGGTTGAAATCATTGAA‑AAAAAAACGCTTAAAGCTATGGTAGATGCCGGAAAGGTGGGTATAAACTTTGGCggtgggtgg                                                                 >  4:741883/1‑136 (MQ=255)
                                                                             tttcacTCTGAATGAAGTTTCGGGTCGCGTCTATCGTGGTGTCGTTGGTTCGCC‑ATAACAGGTTGATATCATTGAA‑AAATAAACGGTTAAAGCTCTGGTAGATGCCGGTCAGGTGGTGATCACCGTTGGCggttgtggtt                                                                <  5:497931/137‑2 (MQ=255)
                                                                                     tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTGGTTGCTTCGGC‑AAAACCGGGTGCTATCATTGAA‑AAAGAAACGGTTAAAGCTGTGGGAGATTCCGGACAGGTGGTGATCACCGGTTGCGGTGGTGGGATGCCAGtt                                                        >  2:869065/1‑140 (MQ=255)
                                                                                     tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTGGTTGCTTCGCC‑AAAACCGGTTGATATAATTGAA‑AAAGAAACGGTTAAAGCTCGGGTAGATTCCGGACAGGTGGTGATAACCGTTGGCGGGGGTGGTATCCCTGtt                                                        >  6:908521/1‑140 (MQ=255)
                                                                                     tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTCGCC‑CAAACAGGTTGATATCATTAAA‑AAAGAAAACGATAAATCTCGGGGATATGCCGGGCAGGTGGTGAGAAACGTTGGCGGAGGTAGGATcccgggt                                                        >  5:308838/1‑136 (MQ=255)
                                                                                     tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGt                                                                                                                     >  3:242706/1‑79 (MQ=255)
                                                                                     tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCGACGCC‑AAAACCGGGTGCTATAATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGTCACGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGtt                                                        >  4:205379/1‑140 (MQ=255)
                                                                                     tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCATCGCC‑CAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTAGGGTAGCTGCCTGACAGGTGGTGAAGACCCTGGGGGGGTGTGGTATCACAGtt                                                        >  2:92989/1‑140 (MQ=255)
                                                                                     tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTTGATCGCC‑CAAACCCGGTGATATCATTTTA‑AAAGAAACGGGTTAAGCTCTGGTTGATTCCGGACAGGTGGTGCTAACCGTTGGCGGTGGTGGTATCACTGtt                                                        >  6:361509/1‑140 (MQ=255)
                                                                                     tGAAAGAAGATGCGGGTCGCGGCTATCGTCGTCTAGTTGATTAGCC‑AAAACCCGGTGAAATCAATGAA‑AAAGAAACCGTTAAAACACTGGTATATGACACACAGGTGGTGATCCCCGATGGGCGTGGTGGTAACCCtaat                                                        >  4:373608/1‑137 (MQ=255)
                                                                                     tGAAAGAAGATGCGGGTCGCGGCTATCCTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGt                                                                                                                     <  4:242706/79‑1 (MQ=255)
                                                                                       aaaGAAGATGCGGGTCGCGGCTATCGTCGTGTAGTTTGTTCGCC‑AAAAACCGTTGATATGATTGTA‑AAACAAAAGGTTAAAGCTCTGGGTGATGCCGGACATGTGGGGATAACCCTTGGCGGGGGTTGTATgccgttttt                                                      >  4:636388/1‑134 (MQ=255)
                                                                                           aagaTGCGGGGCGCGGCTATCGGCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGt                                                  <  3:193164/140‑1 (MQ=255)
                                                                                             gaTGCGGGTCGCGGCTATCGTCGTGTCGTTTCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTTAAGCTCGGGTAGATGCCCGACAGGTGGTGATAACCGGTGGCGGGGGTGGTATCCCTGTTATTAGTGa                                                >  8:269715/1‑140 (MQ=255)
                                                                                             gaTGCGGGTCGCGGCTATCGTCGTGGCGTTGCTTCGCC‑CAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGGAGCTGCCGGCCAGGTGGTGATCACCGGTGGCGGGGGTGGTTTCCCTGTTATTAGTGa                                                >  4:51010/1‑140 (MQ=255)
                                                                                               tGCGGGTCGCGGCTATCGTCGTGTCGTTGGGTAGCC‑CAAACCGGGTGATATGATTGAA‑AAAGAAACGGTTAAAGCTCTGGTTGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTTTGCCTGGTATTAGTGTAg                                              >  1:358788/1‑140 (MQ=255)
                                                                                               tGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggtggt                                                                 >  2:454435/1‑121 (MQ=255)
                                                                                               tGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggtggt                                                                 <  1:454435/121‑1 (MQ=255)
                                                                                                 cGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGt                                            <  6:733760/140‑1 (MQ=255)
                                                                                                 cGGGTCGCGGCTATCGTCGTGTAGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGt                                            <  7:142841/140‑1 (MQ=255)
                                                                                                    gTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAAc                                         <  8:372544/140‑1 (MQ=255)
                                                                                                       gcgGCTATCGTCGTGTCGTTGCTTAGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCAt                                      <  2:901634/140‑1 (MQ=255)
                                                                                                       gcgGCTATCGTCGTGTCGTGGCTTCGCC‑AAAACCGGTTGATATCATAGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCAt                                      <  1:926384/140‑1 (MQ=255)
                                                                                                        cgGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGGTTATATAATTGAA‑AAAGAAACGGTTAAAGCTCTGGTTTATGCCGGGCAGGTGGTGATCACCGGTGGCGGGGGGGGTATCCCGGTTTTTAGTGAATGTAACCaaa                                     >  8:436871/1‑138 (MQ=255)
                                                                                                            tATCTTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCg                                 <  8:874237/140‑1 (MQ=255)
                                                                                                            tATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCg                                 <  5:345801/140‑1 (MQ=255)
                                                                                                            tATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCg                                 <  1:220774/140‑1 (MQ=255)
                                                                                                              tcgtcgTGTAGTTTCTTCGCC‑AAAAACCGTTTATATCATTTAA‑AAACAAACGGTTAAAGCTATTGTATATTCCGGACATGTGGTGATAACCGTTGGCGGTGGTGGTATCCCTGTTATTAGTTAAGGTAAACATCTGAgtg                               >  8:336181/1‑140 (MQ=255)
                                                                                                                     gtcgtTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAg                        <  1:5845/140‑1 (MQ=255)
                                                                                                                       cgtTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAgcg                      <  8:166595/140‑1 (MQ=255)
                                                                                                                       cgtTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAgcg                      <  6:409233/140‑1 (MQ=255)
                                                                                                                       cgtTGCGTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAgcg                      <  6:101337/140‑1 (MQ=255)
                                                                                                                           gCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGt                  >  2:968946/1‑140 (MQ=255)
                                                                                                                             ttCGCC‑AAAACCGGTTTTTTTCATTGAA‑AAAGAAACGTTTAAAGCTCTGGTAGATGCCGGTCAGGTTGTGATCACCGTTGGCGGTGTTGGTATCCCTGTTATTCGTGAATGTAACCATATGCGTGGTGCCAGCGAGGTTa                <  6:499231/140‑1 (MQ=255)
                                                                                                                             ttCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTa                <  7:25655/140‑1 (MQ=255)
                                                                                                                             ttCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGAGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTa                <  2:424406/140‑1 (MQ=255)
                                                                                                                             atCGCC‑AAAACCGGTTTATATCATTGAA‑AAAGAAACGGTTAAAGCTCTTGTAGATGCCGGACAGGTGTTTATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGACAGCGCGGTTa                <  6:202135/139‑1 (MQ=255)
                                                                                                                                    aaaaCCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATa          <  7:326883/140‑1 (MQ=255)
                                                                                                                                    aaaaCCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGAACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATa          <  7:682910/140‑1 (MQ=255)
                                                                                                                                        ccGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTTCGTGGTGCCAGCGCGGTTATCGATAAAGa      >  3:79832/1‑140 (MQ=255)
                                                                                                                                        ccGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGa      <  4:933391/140‑1 (MQ=255)
                                                                                                                                        ccGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGGAACCATCTGCGGGGTGCCAGCGCGGTTATCGATAAAGa      <  2:573922/140‑1 (MQ=255)
                                                                                                                                         cGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGAc     <  4:576474/140‑1 (MQ=255)
                                                                                                                                           gTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTg   >  4:534103/1‑140 (MQ=255)
                                                                                                                                           gTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTg   >  5:604216/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCC‑AAAACCGGTTGATATCATTGAA‑AAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGATAAAGACTGG  >  NZ_CP022686/1632362‑1632640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A