Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F36 I0 R1 149 10545.8 7272710 98.3% 7149073 134.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,632,501 T→G 8.6% V166G (GTT→GGT)  arcC → carbamate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,632,5010TG 8.6% 285.9 / 2.9 162V166G (GTT→GGT) arcCcarbamate kinase
Reads supporting (aligned to +/- strand):  ref base T (50/91);  new base G (15/3);  total (65/97)
Fisher's exact test for biased strand distribution p-value = 1.64e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

GCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGA  >  NZ_CP022686/1632365‑1632630
                                                                                                                                        |                                                                                                                                  
gCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCGTAGCCAAAACCGGtg                                                                                                                                   >  2:285538/1‑137 (MQ=255)
   ttCCTCAACCCGAACCAGCCGAGCGGTTCGTTATTTACCGATCAAGAATAATAGATACTGACAAAAAATGGTTACACTCTGAACTAAGATGCGTGTCGCGTCTATCGTCGTGTCGTTGCTTAGCAAAAACAGGTTGat‑at                                                                                                                             <  5:361590/140‑1 (MQ=255)
        cAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCAt                                                                                                                          >  7:287317/1‑138 (MQ=255)
          aCCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGaa                                                                                                                      <  6:417858/140‑1 (MQ=255)
                ccAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAg                                                                                                                  <  8:719052/138‑1 (MQ=255)
                ccAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAg                                                                                                                  <  1:928334/138‑1 (MQ=255)
                  aaGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTCGCCAAAACCGGGTTAG‑ATCATTGAAAAAGaa                                                                                                                >  3:826746/1‑138 (MQ=255)
                     ccGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTGGGCAAAACCGGTTGATAATCA‑TGAAAAATAAACg                                                                                                             >  7:438109/1‑138 (MQ=255)
                      cGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGtt                                                                                                          <  5:340720/140‑1 (MQ=255)
                        ctCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGtt                                                                                                          <  3:877116/137‑1 (MQ=255)
                        aTCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGtt                                                                                                          <  1:885820/138‑1 (MQ=255)
                        aTCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTaa                                                                                                        >  6:261178/1‑140 (MQ=255)
                         tCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGAAGCGGGTCGCGGATATCGTCGTGTCGTTGAATAGCCAAAAACGCTTTAT‑ATAATTTAAAAATAAAAGTTTaaa                                                                                                       >  6:310144/1‑140 (MQ=255)
                           ggTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTAGTTCGCCAAAACCGGGTGAT‑ATAATTTAAAAAGAAACGGTTaaa                                                                                                       >  1:630815/1‑138 (MQ=255)
                            gttcgttcTTTACCGAGCAAGAAGCAGAGCTACTGACAAAAAAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCTGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAg                                                                                                      <  3:507433/138‑1 (MQ=255)
                                 ttcTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACCCTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGt                                                                                               <  5:44831/140‑1 (MQ=255)
                                 ttcTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAAAGGTTAAAGCTCTg                                                                                                 >  4:362415/1‑138 (MQ=255)
                                 gtcTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGGCGCGGCTATCGTCGTGTCGTTTCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGt                                                                                               <  5:521299/139‑1 (MQ=255)
                                         cGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTGCTTAGCCAAAAACGGTTGAT‑ATCATTGAAAAATAAACGGTTAAAGCTATGGTAGAttc                                                                                         >  1:33288/1‑136 (MQ=255)
                                            tcAAGAAGCAGAGCTACTTACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCgg                                                                                      <  7:245860/137‑1 (MQ=255)
                                            gaaagacgAAGACCTACTGACAAAACTCGGTTACACTCTGAAAGAAGATGAGTTTCGCTGAGATCGTCGTGTCGATGATTCGCCAAAACCGGTTTAT‑ATCATTGAAAAAGAATCGGTTAAAGCTCTGGTAGATGCCgg                                                                                      <  7:125498/136‑1 (MQ=255)
                                            gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCTCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACGGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCGCTGGTAGATGCCgg                                                                                      <  3:208491/138‑1 (MQ=255)
                                            gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCTGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAATCTCTGGTAGATGCCgg                                                                                      <  1:400042/138‑1 (MQ=255)
                                            gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCgg                                                                                      <  1:177047/138‑1 (MQ=255)
                                            gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCgg                                                                                      <  2:139065/138‑1 (MQ=255)
                                            gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCGCTGGTAGATggccg                                                                                      >  4:416623/1‑134 (MQ=255)
                                            gCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGGTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCgg                                                                                      <  8:584107/138‑1 (MQ=255)
                                             cAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTTCCTCGCCAAAACCGGTTGAT‑AACATAGAAAAAGAAACGGATACAGCGCTGGTAGCTTCCggg                                                                                     >  7:223356/1‑137 (MQ=255)
                                             cAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTCCCCAAAACCCGTTGAT‑ATAATTGAAAAAGAAACGGTTTATGCGCTGGTAGATGCCggg                                                                                     >  1:910737/1‑137 (MQ=255)
                                             cAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTAGTTTCTTCGCAAAAAACGGTTGAT‑ATAATTTAAAAAGAAACGGTTAAAGCACTGGTATATGCCggg                                                                                     >  7:224051/1‑137 (MQ=255)
                                             cAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTTCGTGGCCAAAACCGGGTGAG‑TTCATTTAAATAGAAACGGTGAAAGCTCTGGTAGATGCCggg                                                                                     >  3:111020/1‑137 (MQ=255)
                                             cAAGAAGCAGAGCTACGGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCAGAGCCAAAAACCGTTCAT‑ATCAATGAAAAACAAACCGTTAAAGCTCTGGTAGAAGCCggc                                                                                     >  3:685528/1‑137 (MQ=255)
                                                      cagCTACTGACAAAACAGGGGTACACTCTGAAAGTAGATGCGGGTCGAGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGgtggtg                                                                            <  3:511679/137‑1 (MQ=255)
                                                       cgCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGGTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGa                                                                           <  7:286675/137‑1 (MQ=255)
                                                       cgCTACTGAAAAAACAGGGGTACACTCTGAAAGACGATGCGGGTCGCGGCTATCGTCGTGTCTTTGCTTCGCCAAAACCGGTAGAT‑ATCATTGAAAAATAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGa                                                                           <  1:262618/137‑1 (MQ=255)
                                                       agCTACTGTCAATACAGTGTTACTCTCTGAAAGATGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGACAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTATAGCTCTGGTAGATGCCGGACAGGTGGTGa                                                                           <  2:358661/138‑1 (MQ=255)
                                                       agCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTCGCCAAAACCGGTTGAT‑ATAATTTAAAAAGAAACGGTTAAAGCTCTGGTAGATGGCGGCCAGGTGGTGAta                                                                         >  5:723572/1‑139 (MQ=255)
                                                       agCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGa                                                                           <  1:385963/138‑1 (MQ=255)
                                                       agCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATc                                                                         >  5:716522/1‑140 (MQ=255)
                                                       agCTACTGACAAAACAGGGTTACACTCAGAAAGCAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATc                                                                         <  5:625299/140‑1 (MQ=255)
                                                             tGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTAGCCAAAACCGGTTGAT‑ATAAATGAAAAAGAAACGGGTAAATCTGTGGTTGATGCGGGACAGGGGGTGATAACCg                                                                     >  1:257343/1‑138 (MQ=255)
                                                             tGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCGTAGCCAAAACCGGGTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTATGGTAGATGCCGGACAGGTGGTGATAACCg                                                                     >  2:360899/1‑138 (MQ=255)
                                                               aCAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGGGGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGtt                                                                   <  8:308806/138‑1 (MQ=255)
                                                                cAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGGTGAT‑ATCATTGAAAAAGAAACGGTTTAAGCTCTGGTCGATGGCGGACAGGTGGTGATCACCGTTg                                                                  >  4:811805/1‑138 (MQ=255)
                                                                  aaaCAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCgg                                                              <  6:716522/140‑1 (MQ=255)
                                                                  aaaCAGGGTTACACTCTGAAAGAAGATGCGGGGCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGATGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATTCCGGACAGGTGGTGATCACCGTTGGc                                                                <  4:687311/138‑1 (MQ=255)
                                                                   acccgggTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCg                                                               <  4:296274/134‑1 (MQ=255)
                                                                   aaCAGGGTTACACGATGAAAGAAGATGCGGGGCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTAAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggt                                                             <  6:456830/140‑1 (MQ=255)
                                                                     cAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTGGTTGCTTCGCCAAAAACGGTTGTT‑ATAAATTAAAAAGAAACGGTTAAAACTCTGGTAGTTGCCGGACAGGTGGGGCTGACCGTTGggggg                                                             >  7:855413/1‑137 (MQ=255)
                                                                     cAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGTTCGCCAAAACCGGGTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTATGGGTGATGCCGGACAGGTGGTGATCACCGTTGGCggg                                                             >  2:656493/1‑137 (MQ=255)
                                                                     cAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCGTCGCCAAAACCGGTTTAT‑ATCATTGCAAAAGAAACGGGTAAAGCGGTGGGAGAAGCCGGACACGGGGGGATCACCGGTTGCggg                                                             >  3:184065/1‑137 (MQ=255)
                                                                         gTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGGATCTACAAAAAAGGTTGAT‑AAAAATGAAAAATAAAACGGTAAATCTCTGTTAGATGCCGGACATGTTGTGATCAACGGTTGCgttggtggt                                                       >  6:590063/1‑140 (MQ=255)
                                                                         gTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggtg                                                         <  8:716242/138‑1 (MQ=255)
                                                                          ttACACTCTGAAAGAAGATGCGGGTCGCGTCTATCGTCGTGGCGTTTCTTAGCCAACACCGGTTGCT‑ATCATTTAAAAAGAAACGGGGAAAGCAATTGTAGATTCCGGCCAGGGTGTGAACAACGGGGGCcgttgtgg                                                        >  2:453344/1‑138 (MQ=255)
                                                                          ttACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAATGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggtgg                                                        <  2:156363/138‑1 (MQ=255)
                                                                           tACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATAATTGTAAAATAAACGGTTAAAGCGCTGGTAGATGCCGGGCAGGTGGTGATCACCGGTGGCggtggtggt                                                       >  1:651149/1‑138 (MQ=255)
                                                                           tACAATCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTTCTTCGCCAAAAACGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGAGCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCggtggtggt                                                       <  7:343009/138‑1 (MQ=255)
                                                                             cacTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGGTGAT‑ATAAATGAAAAAGAAACGGTTTAAGCTCTGGGAGATGCCGGGCAGGTGGTGATCACCGTTGGCGGGGGTGGTAt                                                     >  2:321434/1‑138 (MQ=255)
                                                                             cacTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCGTCGCCAAAACCCGTTGAA‑ATGAATGAAAAAGAAACCGTTTAAGCGCAGGTAGATGCCCGACACGGGGTGATAACCCGTGGCgggggtggttt                                                     >  7:595767/1‑136 (MQ=255)
                                                                               ctctGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGa                                                                           >  2:276853/1‑114 (MQ=255)
                                                                                    aaaGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCgg                                                                                      <  3:720682/98‑1 (MQ=255)
                                                                                    aaaGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGGGGTATCCCTGtt                                              >  2:590483/1‑138 (MQ=255)
                                                                                    aaaGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGGTAAAGCTCTGGGAGAAGCCGGACAGGTTGTGATCACCGTTGGCGGTGGTTGGATCCCTGtt                                              >  4:416447/1‑138 (MQ=255)
                                                                                    aaaGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCGTCGCCAAAACCGGTTGAG‑ATCAATGAAAAAGAAACGGGTAAAGCACTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGtt                                              >  8:290944/1‑138 (MQ=255)
                                                                                    aaaGAAGATGCGGGTCGCGGCTATCGTCGTGTAGTTTAGTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACCGTTAAAGCTATTTTTGAAGCCCGACAGGATGTGAAAACCCTTGGCGGTTGTGGTTTGCCTGtt                                              >  2:769539/1‑138 (MQ=255)
                                                                                    aaaGAAGATGCGGGTCGCGGCTATCGTCGTGGCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAATAAGAAACGGTTAAAGCTCTGGTAGTTGGCGGACAGGGGGTGATCACCGGTGGCGGGGGGTGTTTCCCTGtt                                              >  2:609538/1‑138 (MQ=255)
                                                                                      agaagaTGCGGGTCGCGGCTATCGTCGTGTCGTTTAGTAGCCAAAACCCGTTGTT‑ATAATTTAAAAAAAAACCGTTTAAGCACGGGGAGGTGGCGGACAAGGGGTGATTACCCGTGGCGGTGGTGGGGTACCCGTTAt                                            >  3:479530/1‑138 (MQ=255)
                                                                                        aagaTGCGTGGCGCGGCTTTCGTCGAGGCGTTGCTTCGCCAAAAGCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTTGTGGTATCCCTGTTATTCGt                                        <  6:632604/140‑1 (MQ=255)
                                                                                        aagaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTc                                          <  7:36169/138‑1 (MQ=255)
                                                                                          gaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCg                                                                                       >  5:454567/1‑91 (MQ=255)
                                                                                          gaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCg                                                                                       <  6:454567/91‑1 (MQ=255)
                                                                                          gaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGt                                        <  8:743229/138‑1 (MQ=255)
                                                                                          gaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGACAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGt                                        <  1:914453/138‑1 (MQ=255)
                                                                                          gaTGCGGGTCGCGGCTATCGTCGTGTCGTTGCTGCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGACACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTTGTATCCCTGTTATTCGt                                        <  7:188871/138‑1 (MQ=255)
                                                                                          gaTGCGGGGCGCGGCGATCGTCGGGTCGTTGCTTCGCCAAAACCGGTTGAA‑ATCATTGAAAAAGAACCGGTTAACGCTCAGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGt                                        <  8:688236/138‑1 (MQ=255)
                                                                                            tGCGGGTCGCGGCTATCGTCGTGTCGTTTCTTCGCCAAAACCGGTTGAT‑ATCATTGTAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGGGATCACCGTTGGCGGTGGTGGTATCCCGGTTATTCGTGa                                      >  1:582741/1‑138 (MQ=255)
                                                                                              cGGGTCGCGGCTATCGTCGTGTGGTTGCTTCGCCCAAACCGGGTGAT‑ATAATTTAAAAAGAAACGGGTAAACCTATGGGAGATGACGGCCCGGTGGTGATCACCGTTGGGGGGGGTGGTATACCGGTTATTAGGGAAg                                    >  2:847759/1‑138 (MQ=255)
                                                                                              cGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAg                                    <  1:775866/138‑1 (MQ=255)
                                                                                              cGGGTCGCGGCTATCGTCGTGGCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAg                                    >  4:75093/1‑138 (MQ=255)
                                                                                              cGGGTCGCGGCTATCGTCGTGGCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTAGCCCTGTTATTCGTGAAGGt                                  <  5:533620/140‑1 (MQ=255)
                                                                                               ggggCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAgg                                   <  4:348215/138‑1 (MQ=255)
                                                                                                ggTCGCGGCTATCGTCGTGGCGTTGGTTGGCCAAAACCGGGTGAT‑ATCATTGAAAAAGGAACCGTTTAAGCGCTGGTAGATGCCGGCCAGGTGGGGGTCACCGGTGGGGGGGGTGGGTTGCCTGGTATTGGTGGAggg                                  >  7:860895/1‑137 (MQ=255)
                                                                                                   cgcgGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGGTGAT‑TTCATTGAAAAAGAAACGGGTAAAGCTCTGGGTGATGCCGGCCAGGTGGTGATAACCGTTTGGGGTGGTGGTATCCCGGGTATTAGTGTAGGTTACCa                             >  6:160106/1‑140 (MQ=255)
                                                                                                         tATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTg                         <  7:297239/138‑1 (MQ=255)
                                                                                                         tATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTg                         <  7:384081/138‑1 (MQ=255)
                                                                                                         tATCGTCGTGTCGTTGCTTCGCCAAAACCGCTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTg                         <  8:374279/138‑1 (MQ=255)
                                                                                                         tATCGTCGTGTCGTTGCTTCGCAAAAACCGTTTGAT‑ATCTTTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTg                         <  7:757793/138‑1 (MQ=255)
                                                                                                         tATCGTCGTGTCCGTGCGTCGCCAAACCCGGGTGAT‑ATCATTGCAAAAGAACCGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTg                         <  3:270390/138‑1 (MQ=255)
                                                                                                                  gtggatGCTTCGCCAAAACCGGCTGAT‑ATCATTGATAAAGATCAGGTTTAAGCTCTGGTAGATGCCGGACATGCGGTGATCACCGTTGGCGGTGGGGGTATCCCAGTTATTCGTGAAGGTAACCATCTGCGTTGTGcc                <  2:126144/133‑1 (MQ=255)
                                                                                                                    ggtTGCTTCGCCAAACCCGGATGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAg              <  8:688643/137‑1 (MQ=255)
                                                                                                                    ggtTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAgcg            <  5:160106/139‑1 (MQ=255)
                                                                                                                    cgtTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGc                                                                <  1:206531/88‑1 (MQ=255)
                                                                                                                    cgtTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGc                                                                >  2:206531/1‑88 (MQ=255)
                                                                                                                    cgtTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAgcg            <  5:512623/140‑1 (MQ=255)
                                                                                                                          ttCGCCATAATCTGTTGAT‑ATCATTGAAAAAGAATCTGTTTAAGATCTTGAAGTTGCCGGACAGGTGGTCCTCACCTTTGGCGGTGTTGGTATCCCTGCTTTTGTGGAAGGGAACCATCTGCGATGTGCCAGCGCGGt        <  3:687343/138‑1 (MQ=255)
                                                                                                                          ttCGCCAAAACCGGTTGAT‑CTCATTGAAAAAGAAAGGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGt        <  7:518790/138‑1 (MQ=255)
                                                                                                                          ttCGCCAAAACCGGTTGAT‑ATCATTGAATAATAAACTGTTAAAGCTATGGTAGATGCCGGACATGTGGTGATCACCGCTGGCTGTGGTGGTATCCAAGTTATTCGTGAAGGTAACCATCTTAGTGGTGCCAGCGCGGt        <  2:332393/138‑1 (MQ=255)
                                                                                                                          ttCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAATGTAACCATCTGCGTGGTGCCAGCGCGGt        <  7:363856/138‑1 (MQ=255)
                                                                                                                               cAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCg   <  3:127556/138‑1 (MQ=255)
                                                                                                                                aaaaCCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGa  <  1:764343/138‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                  
GCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCTACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCGGGTCGCGGCTATCGTCGTGTCGTTGCTTCGCCAAAACCGGTTGAT‑ATCATTGAAAAAGAAACGGTTAAAGCTCTGGTAGATGCCGGACAGGTGGTGATCACCGTTGGCGGTGGTGGTATCCCTGTTATTCGTGAAGGTAACCATCTGCGTGGTGCCAGCGCGGTTATCGA  >  NZ_CP022686/1632365‑1632630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A