Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F12 I0 R1 131 9881.0 7547414 98.3% 7419107 134.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,224 T→G 16.4% K188N (AAA→AAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2240TG16.4% 220.3 / 13.0 135K188N (AAA→AAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (21/89);  new base G (22/3);  total (43/92)
Fisher's exact test for biased strand distribution p-value = 1.25e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

CTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGT  >  NZ_CP022686/1674087‑1674354
                                                                                                                                         |                                                                                                                                  
cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCGGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGtgt                                                                                                                                  >  3:37676/1‑138 (MQ=255)
  ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCATGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCACCTTACATTTTTTGTTTTTAGTGTAGAGTAGGttttt                                                                                                                                >  3:260071/1‑140 (MQ=255)
   gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGTCCTGACGGCGACGCTGATACTCTTGCCGTGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTg                                                                                                                               <  3:649716/140‑1 (MQ=255)
   gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGtttt                                                                                                                                 <  6:188937/138‑1 (MQ=255)
   gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGtttt                                                                                                                                 <  7:260788/138‑1 (MQ=255)
   gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGtttt                                                                                                                                 <  6:783503/138‑1 (MQ=255)
     gcagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGATTCGGtttttt                                                                                                                               >  2:263116/1‑137 (MQ=255)
        gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCtt                                                                                                                          >  3:302942/1‑140 (MQ=255)
                  gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGcggt                                                                                                                <  3:163731/140‑1 (MQ=255)
                        cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAGTTGTGGGGcggtcggggg                                                                                                          >  3:369115/1‑137 (MQ=255)
                           tGCCTGGTTGCATTTGCTCTACCAGGGCCTGACGTCGACGCTGAAACTCTTGCCGGGATATATCAATCATAACACTCTCCTTACATTTTTTGTTTTTTGTGTTGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                         <  7:163792/138‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTAGCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTTGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGctg                                                                                                       <  3:318339/140‑3 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGTGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCTGTCGGTTGc                                                                                                         <  1:843494/138‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                         <  2:685865/138‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                         <  7:96747/138‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                         <  6:705714/138‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                         <  7:652314/138‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                       <  4:264378/140‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                       <  3:430869/140‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGGCGGTTGc                                                                                                         <  8:282741/138‑1 (MQ=255)
                           tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCATACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGGCGGTTGc                                                                                                         <  2:42449/138‑1 (MQ=255)
                           tGCCGGGTTGCATTTGATCTACGAGGGCCTGCCGGGGAAGCTGAAACTGTTGCCGGTATATATCTCTCATAACACTCTCCTTAAATTTTTTGTTTTTATTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                         <  7:712450/138‑1 (MQ=255)
                           ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                         <  5:214225/137‑1 (MQ=255)
                           ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                         <  2:482711/137‑1 (MQ=255)
                             ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                       <  2:630555/138‑1 (MQ=255)
                             ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                       <  1:933015/138‑1 (MQ=255)
                             ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                       <  6:66319/138‑1 (MQ=255)
                             ccGGGTTGCAGTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCTATCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                     <  4:836145/140‑1 (MQ=255)
                              cGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCGGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGGCGGTTGCGGa                                                                                                      <  7:170337/138‑1 (MQ=255)
                                  ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCAGGAGCGGGCGGGTGCGGATGAGTa                                                                                                >  4:3756/1‑140 (MQ=255)
                                   tGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGt                                                                                                 <  2:784237/138‑1 (MQ=255)
                                     cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGAGGTGGGTTTCGGATGAGTTAAg                                                                                             >  4:452246/1‑140 (MQ=255)
                                         tgttctACCAGGGCCTGTAGGCGACGCTGTAACTCTTGCCGGTATATATCACTCATTAAACTCTCCTTTCATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCTCGTGCTGTTGCGGATGAGTAAAGgt                                                                                           <  8:53204/135‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGTCGACGCTGAAACTCTTGCCGGTATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                         <  4:600711/140‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                         <  4:630653/140‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                           <  7:624997/138‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                           <  2:196769/138‑1 (MQ=255)
                                         tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGCTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                           <  7:476418/138‑1 (MQ=255)
                                         acagctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                           <  7:411979/134‑1 (MQ=255)
                                           ctctACCAGGGCCTGACGGTGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                       <  4:180109/140‑1 (MQ=255)
                                           ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGTATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                         <  8:827185/138‑1 (MQ=255)
                                           ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGTGGATGAGTAAAGGTGTCg                                                                                       <  3:781150/140‑1 (MQ=255)
                                           ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                         <  5:778650/138‑1 (MQ=255)
                                           ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                         <  5:337144/138‑1 (MQ=255)
                                           ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                         <  1:141928/138‑1 (MQ=255)
                                           ctctACCAGGGCCGGACGGCGACGATGAACCTCTTGCCGGTATATATCAATCATAACACTCTCCTTACATTTTTTGTTTTTAGATTAGAGTCGGTTATTGTACTTCTGGCGCTGGCGGTAGCGGATGAGTAAAGgtgt                                                                                         <  2:876633/138‑1 (MQ=255)
                                                 cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTTGAGTCGGTTTTTGTTCTTCTGGCGCGGGGGGTTGGGGTTTAGTTAAAgtgtgggggg                                                                                   >  5:627412/1‑132 (MQ=255)
                                                 cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTGTTGTGGCGCGGTGGGGTGGGGTTGTGTAAAGGTGtggtggg                                                                                   >  7:37013/1‑137 (MQ=255)
                                                 cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTAATTGTTGCGCGGGGGGGTGGGGATGAGTTAAGGTGtggtggg                                                                                   >  5:500743/1‑137 (MQ=255)
                                                      ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAAc                                                                              <  1:888388/138‑1 (MQ=255)
                                                        tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcg                                                                          <  4:579012/140‑1 (MQ=255)
                                                          aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGGTATTCTGGCGGGGTCGGTTGGGGTTGTGTTAAGGTGTCGTGGGATAACAgcg                                                                          >  7:733094/1‑138 (MQ=255)
                                                          aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTACGTCGGGCGCGGGCTGTTTGGGATGAGGAAACGTGTCGTGGCATaaacgcg                                                                          >  5:466920/1‑133 (MQ=255)
                                                           cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcgc                                                                         <  8:682594/138‑1 (MQ=255)
                                                                aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                    <  5:413614/138‑1 (MQ=255)
                                                                aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                  <  4:357770/140‑1 (MQ=255)
                                                                  gCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                  >  8:302184/1‑138 (MQ=255)
                                                                       aaCTCTGTCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGCGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAGGAGTAAAGGTGTCGTGGGATAACAGCGCGGCAACACGGACGt                                                           <  4:166639/140‑1 (MQ=255)
                                                                            ttGCCGGGAGATATCACTCATAACACTCTCCTTACATATTTTGTTTTTAGTGTAGAGTCGGTGTTTGTGGTTATTGAGCGGTCAGGTTCGGATGAGTAAATGTGTCGTGGCATAACAGCGCGGCAACACGGACGTTCTCg                                                      >  4:255983/1‑140 (MQ=255)
                                                                              gCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAAAACCGCGGCAACACGGACGTACtag                                                      >  2:680761/1‑136 (MQ=255)
                                                                               ccGTGATATATCACTCATCACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTATAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCga                                                     <  5:286838/138‑1 (MQ=255)
                                                                                cGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTCTTCTTGCGCGGGCGGTGGCGGATGAGTTAATGTGTCGTGGGATAACAACGCGGCAACACCGACGTTCTCgat                                                    >  7:777939/1‑138 (MQ=255)
                                                                                 gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                   <  6:682962/138‑1 (MQ=255)
                                                                                 gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                   <  6:229344/138‑1 (MQ=255)
                                                                                    atatatCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTATTCTGGGGCGGTGGGGTGCGGATGAGTAAAGGTGTCGTGGCCTAAAAGCGCGGCAACACCGACGTACTGGATGAacta                                              >  3:735962/1‑139 (MQ=255)
                                                                                      atatCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTAGTTATGGCGCGGTCGGTTGGGGTTGAGTAAATGTGTCGTGGGCTAACACCGCGGCAACCCGGACGTACTCGATGAtcta                                              >  1:241798/1‑137 (MQ=255)
                                                                                            cTCATACAACTATCCTATCTTTTTTTTTTTTTTGTTTAGAGTCGGTTTTTGTAATTCTGCCGGGGTCGGTGGCGGATGAGTAAATGTGTCGGTGCATAACAGCGCGGCAACACGGACGAACTCTATTTTCTATTAAAGCg                                      <  4:674835/140‑1 (MQ=255)
                                                                                            cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAg                                        <  7:148720/138‑1 (MQ=255)
                                                                                             tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGc                                       <  8:200424/138‑1 (MQ=255)
                                                                                             tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGc                                       <  5:882561/138‑1 (MQ=255)
                                                                                              actAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGGTGCTGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                      <  2:40949/136‑1 (MQ=255)
                                                                                               aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTACTTATGGCGCGGTCGGTTGCGGATGAGTAAAGTGGTCGTGGGATAACACCGCGGCAACACGGACGTACTCGATGATCTCTTCAACCGACa                                   >  4:519724/1‑140 (MQ=255)
                                                                                                   cacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTATGGCGCGGTGGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCCATGATCTCTTCAAGccgcaat                                 >  2:48660/1‑132 (MQ=255)
                                                                                                    cctctCCTTACATTTTTTGTTTTTAGTGTAGTGGCGGTTTTTGTTCTTCTGGCGCGGTCGGTTGCGGATGAGTAAATGTGGCGTGGGATAAGAGAGCGGCAACTCGGACGTACTCGATTAGCTCTTCAAGCGACATTTCa                              <  4:892411/139‑1 (MQ=255)
                                                                                                    acTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGGACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                <  7:306505/138‑1 (MQ=255)
                                                                                                      tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                              <  5:97046/138‑1 (MQ=255)
                                                                                                       ctcCTTACATTTTTTGTTTTTAGTGTAGCGTCTGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                             <  8:557359/138‑1 (MQ=255)
                                                                                                       ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGGGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                             <  1:368893/138‑1 (MQ=255)
                                                                                                        tcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGct                          <  3:561185/140‑1 (MQ=255)
                                                                                                         ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTCTTGGGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTGGTGGCATAACAGCGCGGCAACCCGGCCGTACTCGATGATGTATTCAAGCGACATTTaaacc                           >  5:270447/1‑136 (MQ=255)
                                                                                                         ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTATGGCGCGGTCGGTTGCGGATGAGTTAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATATCTTAAAGCGACAATTCAAGc                           >  6:97046/1‑138 (MQ=255)
                                                                                                         ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGTCGCGGGCGGTTGCGGATGAGTAAACGTGTAGTGGCATAACATCCCGGCAACACGGACGTACTCGAAGATCTCTTAAAGCGACATTTCaaac                           >  8:249997/1‑136 (MQ=255)
                                                                                                         ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACGTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGGGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGctc                         >  4:361835/1‑140 (MQ=255)
                                                                                                         ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTAATTAGGGCGCCGGGGGGGGCGTCTTATTATAGGTGGGGTGGCAAAACACCGCCGCCACCCGGGCGAACTCGTGTATCAATTAAAGCTACATTTAAAGc                           >  6:492202/1‑138 (MQ=255)
                                                                                                         ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGGGTTTGTTATTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGGGTCGGGGCAGAACAGCGCGGCACCCCCGCCGTACTCGAAGAACTGGTCAAGCGCCAATTAAAGc                           >  7:93017/1‑138 (MQ=255)
                                                                                                          cTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTACTGGGGCGGGCGGTTGCGGTTGAGTAAAGGTGTCGTGGCATAAAAGCGCGGCCACCCCGCCGGACTCGATGAACTCTTAAACCGCCAATTaaaccacg                        >  4:463260/1‑135 (MQ=255)
                                                                                                           ttACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGTCGGTTGAGGATTAGTTAAGGTGTCGTGGCAAAAACGCGCGGCACCACCGACGTACTCGATGATCACTTAAAGCGACATTTAAAGctg                         >  8:406818/1‑137 (MQ=255)
                                                                                                             aCATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCtt                       <  2:923168/138‑1 (MQ=255)
                                                                                                             aCATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                     <  4:28874/140‑1 (MQ=255)
                                                                                                                ttttttGTTTTTAGTGTAGAGTCGGGTTTTGTACTTAAGGCCCGGTCGGATGGGGATGAGTAAATGTGTCGTGGACTAACAGCCCGGCAACACCGACGTAAACGATTCTCTCATAAAGAGACAATTCAAGATCATCCTgt                  >  4:524241/1‑139 (MQ=255)
                                                                                                                  ttttGTTTTTAGTGTAGAGTCGGTTTTTTTAATTATGGCGCGGTCGGGTTGGGATGAGGAAAGGGGTAGTGGCAAAACAGCGCGGCACAACGGCCGGACTCGCAGTTCTCTTCACTCGACATTTAAAGCTATTACAGAta                >  4:881380/1‑139 (MQ=255)
                                                                                                                    ttGTTTTTAGTGTAGAGTCGGGTTTTGTAAATATGGCGCGGTGGGGTTCGGATGAGGAAAAGTGTAGTGGGCTAACCGAGCCGCCACCCGGACTTACTCGATGTTCTGTTCAACCGACCATTCAAACTCGTccgtcgg                >  2:790277/1‑133 (MQ=255)
                                                                                                                         tttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgt           <  1:523888/138‑1 (MQ=255)
                                                                                                                         tttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGGTGCGGTTGAGTATAGGTGTCGTGGCCTAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgt           <  2:98899/138‑1 (MQ=255)
                                                                                                                          ttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtc          <  6:552949/138‑1 (MQ=255)
                                                                                                                             gtgtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc       <  6:175719/138‑1 (MQ=255)
                                                                                                                             gtggAGAGTCGGTTTTTGTACTTCTTGAGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGAGACATTTCAAGCTCTTCCTGAtcttcgtcttc       <  8:566503/138‑1 (MQ=255)
                                                                                                                             gggtAGCGTCGGTTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc       <  8:516537/136‑1 (MQ=255)
                                                                                                                               gtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt     <  1:110456/138‑1 (MQ=255)
                                                                                                                                  gagTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTcgtcgt  <  6:580460/138‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
CTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGT  >  NZ_CP022686/1674087‑1674354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A