Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A27 F36 I0 R1 164 7919.5 7665810 98.4% 7543157 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,224 T→G 10.0% K188N (AAA→AAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2240TG10.0% 338.5 / 7.5 175K188N (AAA→AAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (42/110);  new base G (19/3);  total (61/114)
Fisher's exact test for biased strand distribution p-value = 1.69e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

TTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTA  >  NZ_CP022686/1674085‑1674355
                                                                                                                                            |                                                                                                                                   
ttCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGt                                                                                                                                     <  3:3871/140‑1 (MQ=255)
  cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGttt                                                                                                                                   >  7:376827/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTTCATTTTTTGTTTTTAGTGTAGAGTC‑GGttttt                                                                                                                                 >  7:460491/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGttttt                                                                                                                                 >  8:140702/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGgtttt                                                                                                                                 >  7:571305/1‑140 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGgttttt                                                                                                                                >  7:867545/1‑139 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTg                                                                                                                                <  5:796623/140‑1 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTg                                                                                                                                <  1:450236/140‑1 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTg                                                                                                                                <  4:53036/140‑1 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCAATTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTg                                                                                                                                <  2:648871/140‑1 (MQ=255)
       gcagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGtt                                                                                                                              >  1:671053/1‑139 (MQ=255)
         agcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGCCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACt                                                                                                                            >  3:219115/1‑140 (MQ=255)
          gcaAAAATCAGCGCGGCGCTGCCTGGTTGCATTTGCTCTACAAGGGCCTGACGCCGACGCTGAAACTCTTTCCGGGATATATAACTCATAACACTAGCCTTTCATTTTTTGTTGTGAGTGTAGAGGC‑GGGTTTTTTACtt                                                                                                                           >  2:224974/1‑140 (MQ=255)
          gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTg                                                                                                                                >  7:598776/1‑135 (MQ=255)
          gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTGACtt                                                                                                                           >  1:301312/1‑140 (MQ=255)
          gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACtt                                                                                                                           >  6:149441/1‑140 (MQ=255)
                aTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGgcg                                                                                                                     <  4:18859/140‑1 (MQ=255)
                aTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGgcg                                                                                                                     <  5:446666/140‑1 (MQ=255)
                    gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGcggt                                                                                                                 <  5:242126/140‑1 (MQ=255)
                    gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGcggt                                                                                                                 <  6:662497/140‑1 (MQ=255)
                    gcgcGGAGCTGCCGGGGTTGATTTGATATACCATGGCCTGACGGCGACGCTGAAACTCTTTCCGGGATATATCACTCATAACTCTCTCCTTACATTTTTTGTTTTTAGTGTATAGTC‑GGTTTTTGTACTTCTGGCGcggt                                                                                                                 <  1:239768/140‑1 (MQ=255)
                      gcggcgCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGcggtcg                                                                                                               <  5:577043/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                        <  4:696649/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                        <  4:897030/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                        <  4:218793/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                        <  6:274996/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                        <  2:860731/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                        <  6:783278/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                        <  1:264374/140‑1 (MQ=255)
                             ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                        <  2:424354/139‑1 (MQ=255)
                               ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                      <  5:149441/140‑1 (MQ=255)
                               ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                      >  4:369392/1‑140 (MQ=255)
                               ccGGGTTGCATTTGCTCTACCAGGGACTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                      <  4:163183/140‑1 (MQ=255)
                                cGGGTTGCATTTGCGCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATg                                                                                                     <  2:315267/140‑1 (MQ=255)
                                      gCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTaaa                                                                                               <  4:911737/140‑1 (MQ=255)
                                      gCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTaaa                                                                                               <  4:795070/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                          <  8:834425/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                          <  4:846127/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                          <  8:398328/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                          <  8:452127/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                          <  2:309138/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                          <  3:607280/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                          <  2:700850/140‑1 (MQ=255)
                                            gCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTTATTCTGGCGCGGTCGGTTTGGGATTTGTaaatgggtg                                                                                         >  1:532438/1‑134 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACTCTGAAACTCTTGCCGGGATATATAACTCATAACACTGTCATTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                        <  4:164522/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                        <  8:419440/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                        <  5:676536/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTTCTTAGGGGGCGGTCGGGTGGGGATGTGTAAAAGTGTCg                                                                                        >  3:437936/1‑140 (MQ=255)
                                                  ccAGGGCCTGACGGCGACGCTGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTGAAATTTTGG‑GGGGTTTGTTGCGGATTTGTATATGTGTGGTGTCa                                                                                   >  3:855163/1‑140 (MQ=255)
                                                      ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTACTTAAGGCGCGGGCGGTTGCGGATGAGTAAAAGTTTCGAGGCATAAc                                                                               >  8:758575/1‑140 (MQ=255)
                                                       gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACGTGTTGCGCGGTCGGTTTCGGTTGAGTAAAGGGGTCGTTGAATAAcc                                                                              >  5:324006/1‑139 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTACTTATGGCGGGGTGGGTTGCGGATGAGTTAAGGTGTCGTGGCATAAAAgcgag                                                                         >  8:455013/1‑138 (MQ=255)
                                                             cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                        <  6:861528/140‑1 (MQ=255)
                                                                cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                     <  6:740090/140‑1 (MQ=255)
                                                                cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTGTTTGTTAATATGGGCGGGTCGGTGGCGGAATAGGAAATGGGTAGTGGGATAACAGCGCGGCaa                                                                     >  3:407528/1‑140 (MQ=255)
                                                                 gACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGGGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAac                                                                    >  4:925382/1‑140 (MQ=255)
                                                                  aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                   <  8:603229/140‑1 (MQ=255)
                                                                  aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                   <  6:193958/140‑1 (MQ=255)
                                                                  aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                   <  2:985803/140‑1 (MQ=255)
                                                                  aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                   <  6:744219/140‑1 (MQ=255)
                                                                   cGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAacac                                                                  <  5:775405/140‑1 (MQ=255)
                                                                    gCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACg                                                                 <  1:119730/140‑1 (MQ=255)
                                                                     cTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACgg                                                                <  8:802131/140‑1 (MQ=255)
                                                                      tGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTTCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTGGTGGCATAACAGAGCGGCAACACggc                                                               >  1:873181/1‑139 (MQ=255)
                                                                        aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACg                                                             <  8:558704/140‑1 (MQ=255)
                                                                        aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACg                                                             <  4:638047/140‑1 (MQ=255)
                                                                                 ccGTGATATATCACTCATAACACTCTCATTACATTTTTTGATTTTAGTGTAGAGTC‑GGATTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATATCAGCGCGGCAACACGGACGTACTCgatg                                                    <  6:446262/140‑1 (MQ=255)
                                                                                   gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                  <  1:211894/140‑1 (MQ=255)
                                                                                   gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                  <  5:789820/140‑1 (MQ=255)
                                                                                      atatatCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGAtctc                                               <  3:902247/140‑1 (MQ=255)
                                                                                         aatCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTc                                            <  4:149433/139‑1 (MQ=255)
                                                                                            aaCTAATCACACTCTCCTTTCATTTTTTGTTTTTTGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGTTTGCGGATGAGTAAAGGGTTCGTGGCATATCAGCGCGGCTACACTGAGGTACTCGATGATCTCTTCaat                                         <  6:902722/139‑2 (MQ=255)
                                                                                              cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTTCTTATTGCGCGGTCGGTTTGGGATGAGTTAATGTGTAGTGGCATAACAGCGCGGCAACAAGGACCTAATAGAAGATCTCATAAAgag                                       >  6:936390/1‑138 (MQ=255)
                                                                                              cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCCTAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                       <  2:278400/140‑1 (MQ=255)
                                                                                              cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                       <  7:640998/140‑1 (MQ=255)
                                                                                               tCATACCTCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                      <  2:621593/140‑1 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGTCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                      <  7:179569/140‑1 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                      <  2:242607/140‑1 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTAGCCGATTAGTAAAGGTTTAGTGGCAAAACACCGCGGCAACACGGGCGTTCTGCAAGATGTCTGAAAGCgc                                      >  2:512182/1‑139 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTA‑GGTTTTTGGACTTCTTGGGCGGTCTGTTTCGGTTGTAGAAATGGGTCGTGGCCTAACACGGCCGGAACACTGACGTTCTAGGTGGTCTCTTCAAGCGa                                      >  6:684827/1‑140 (MQ=255)
                                                                                               tCATAACACTCTCCTTACAATTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                      <  6:216575/140‑1 (MQ=255)
                                                                                               acATAACACTCTCCTTACATTTTTTGTTTTTACTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACATCGCGGCAACACGGACGTACTCGATTATCTCTTCAAGCGa                                      <  6:95815/139‑1 (MQ=255)
                                                                                                 aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTTCATATGGCGCGGTGGGTTGGGGATTTGTTAAGTTGTCGTGGGATAACAGCGCGGCACCACCGGAGGACACGATGATCTCTTAAAACGTCa                                    >  3:245569/1‑140 (MQ=255)
                                                                                                 aTAACACTCTCCTTACATTTTTTGGTTTTAGTGTAGAGTC‑GGGTTTTGTTCTTATGGGGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGCCGTACACGATGATCACTTCCAGAGACa                                    >  8:222304/1‑140 (MQ=255)
                                                                                                   aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTTATTATGGCGCGGTCGGTTGCGGATGAGTAAAGGTTTGGTGGCATAACAGGGCGGCAACACGGACGTAATCGATGATCTCGTCAAGCGACAtt                                  >  3:790857/1‑140 (MQ=255)
                                                                                                    acacTCTCCTTTCATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                 <  4:658040/140‑1 (MQ=255)
                                                                                                    acacTCTCCTTACATTTTTTGTTTTTCGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                 <  7:231881/140‑1 (MQ=255)
                                                                                                    acacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                 <  1:919094/140‑1 (MQ=255)
                                                                                                     cacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTc                                <  8:32026/140‑1 (MQ=255)
                                                                                                        tctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTATGGGGCGGTGGGTTTGGGATGGGTTTAGGTGTCGGGGGATAAAAGCGCGGCCACACGGACGTGCTCGATGATCGATTAAAGCGGCATTTGAAg                             >  2:836203/1‑140 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                            <  1:690286/140‑1 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACATCTGGCGCGGGCGGTTGCGGATGAGAAAAGCTGACGTGGCATAACAGCCCGGCAACCCGGAAAAACACGATGATCACATCAAGCAACAATTCAAGc                            >  8:689854/1‑140 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTTCTTCATGGGCGGTCGGTTTCGGATGAGTAAAGGTTTCGTGGCAAAACAGCGCCGCAACACCGACCTACTAGAAGATCTCTTCAAGCGACATTTCAAGc                            >  4:680265/1‑140 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTCTGGCGCGGGCGGTTGGGGATGAGTAAATGTGTCCTGGCCAAACAGCCCGGCCACACGCACGTACGCGAATATATCTACAAGCGACCTTTaaacc                            >  8:265663/1‑138 (MQ=255)
                                                                                                                cATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCt                     <  4:465215/140‑1 (MQ=255)
                                                                                                                    ttttGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTAAGGGGCGGGCGGGTTGGGAGCTTTAAAAGTGTCGGGGGCTAAAACCCCCGCAACACGGCCGGAAACGATGAACGCTTAAAGCGAAAAATAAACCTCTTccgtatg                 >  2:933221/1‑135 (MQ=255)
                                                                                                                           tttAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtct          <  4:203968/140‑1 (MQ=255)
                                                                                                                            ttAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTTGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt         <  6:570467/140‑1 (MQ=255)
                                                                                                                              agggtAGAGGC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCTGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGAGGATCTCATCCAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttcg       <  5:48131/137‑1 (MQ=255)
                                                                                                                               gtgtAGAGGC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt      <  5:550945/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                   
TTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTA  >  NZ_CP022686/1674085‑1674355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A