Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F2 I0 R1 74 6379.7 5468672 98.4% 5381173 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,224 T→G 14.3% K188N (AAA→AAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2240TG14.3% 183.1 / 5.8 114K188N (AAA→AAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (30/63);  new base G (16/3);  total (46/68)
Fisher's exact test for biased strand distribution p-value = 4.75e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

TTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAA  >  NZ_CP022686/1674085‑1674361
                                                                                                                                           |                                                                                                                                           
ttCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGt                                                                                                                                             <  3:92081/140‑1 (MQ=255)
  cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACCCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGt                                                                                                                                             >  8:121107/1‑138 (MQ=255)
  cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGt‑tt                                                                                                                                          >  1:347713/1‑140 (MQ=255)
  cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGCGATATATCACTCATAACACTCTCCTTACAATTTTTGTTTTTAGTGTAGAGTCGGt‑tt                                                                                                                                          >  1:149107/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTCCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACAGTTTTTGTTTTTAGTGTAGAGTCGGt‑tt‑tt                                                                                                                                       >  4:536460/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATCACACTCTCCTTACATTTTTTGTTTTTAGTGTTGAGTCGGt‑tt                                                                                                                                          >  8:596967/1‑138 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTTGAGTCGGg‑tt‑tt                                                                                                                                       >  3:504887/1‑140 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACCCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGg‑tt‑ttt                                                                                                                                      >  4:475681/1‑139 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTg                                                                                                                                      <  1:59321/140‑1 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGAGATATCAATCATAACCCTCTCCTTACATTTTTTTTTTTTAGTGTAGAGTCGGT‑TT‑TTg                                                                                                                                      >  6:253896/1‑140 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCGGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTg                                                                                                                                      <  3:113766/140‑1 (MQ=255)
          gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTaagt                                                                                                                                 >  6:657382/1‑137 (MQ=255)
            aaaaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑GT‑TTGttatt                                                                                                                                 >  7:464643/1‑134 (MQ=255)
            aaaaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACAAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTGT‑TT‑TTGttgtt                                                                                                                                 >  7:266625/1‑134 (MQ=255)
                aTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTgg                                                                                                                             <  8:403453/138‑1 (MQ=255)
                     cgcgGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTATGGCGcgggg                                                                                                                      >  1:362003/1‑138 (MQ=255)
                     cgcgGCGCTGCCGGGTTGCATTTGCGCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCGTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTATGGGGcgggc                                                                                                                      >  3:450446/1‑138 (MQ=255)
                     cgaggcgCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGcggtc                                                                                                                      <  6:263229/137‑1 (MQ=255)
                       cggcgCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑GT‑TTGTTCGTGTGGCgcgggcgg                                                                                                                    >  1:327679/1‑140 (MQ=255)
                        ggcgCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGcggtcggt                                                                                                                   <  1:9232/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCATGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  1:687282/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTAGATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  5:79248/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  6:37724/140‑1 (MQ=255)
                             tGCCGGGGTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGc                                                                                                                <  8:424349/138‑1 (MQ=255)
                             ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGGCGGTGGCgg                                                                                                              <  6:145523/139‑1 (MQ=255)
                              gCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGa                                                                                                             <  5:100343/140‑1 (MQ=255)
                               ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  7:162729/138‑1 (MQ=255)
                               ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                            <  5:512861/140‑1 (MQ=255)
                               ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                            >  4:441918/1‑140 (MQ=255)
                                cGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATg                                                                                                           <  2:367765/140‑1 (MQ=255)
                                cGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATg                                                                                                           <  3:307903/140‑1 (MQ=255)
                                    ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTATGGCGCGGTCGGGTGCGGATGAGtt                                                                                                       >  3:435515/1‑139 (MQ=255)
                                       cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTaa                                                                                                      <  8:397173/138‑1 (MQ=255)
                                       cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTCTTGCGCGGTCGGTTTCGGATGAGTaaaa                                                                                                    >  5:360355/1‑139 (MQ=255)
                                       cATTTGCTCTACCAGGGCCGGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAg                                                                                                    <  4:688951/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  6:581319/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  4:424193/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  3:81903/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  3:441918/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  5:641224/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGAAGTCGACGCTGAAACTCTTGCCGGTATATATCACTCATAACACTCTCCTTACATATTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTCCTTCTGGCGCGTTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  5:89572/140‑1 (MQ=255)
                                            gCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTTCTTGGGGCGCGGTCGGTTGGGGATGAGTAAATgtgtg                                                                                               >  4:202165/1‑139 (MQ=255)
                                             gtctACCAGGGCCTGACGGCGACGCTGTAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                              <  1:398246/139‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATGACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                              <  5:44724/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  7:104973/138‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  7:562090/138‑1 (MQ=255)
                                                 aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTCTGGCGCGGTGGGGTGCGGGTGGTGTAAGGTTTCGTggg                                                                                          >  3:334697/1‑139 (MQ=255)
                                                 aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTACTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGTGGCGGGCCGGTGAGGAATAGTTAAGGTGTCGTGGc                                                                                          >  6:538662/1‑140 (MQ=255)
                                                   cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCAt                                                                                        <  6:400643/140‑1 (MQ=255)
                                                      ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCCTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAAc                                                                                     <  5:434823/140‑1 (MQ=255)
                                                       gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑GT‑TTGTACTTATGGCGCGGGAGGTTGCGGATGAGTTTAGGTGTCGTGGGATAACa                                                                                    >  4:640288/1‑140 (MQ=255)
                                                       gCCAGACGGCGACGCTGAAAATCTTGCCGGGATATATCACTCATACCACTCTCCTTACATTTTTTGTATTTAGTGTATAGTCGGTGTT‑TTGT‑CATGTGGCGCGGGCGGTTGGGGATGAGGAAAGGGGTCGGGGCAGAAcc                                                                                    >  4:49774/1‑139 (MQ=255)
                                                        ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                   <  2:347713/140‑1 (MQ=255)
                                                        ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                   <  1:602651/140‑1 (MQ=255)
                                                          tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTTTTATTGAGGCGCGGGCGGGTGGGGGTTATTAAAGGGGTGGGGGGATAACAgcg                                                                                 >  2:45535/1‑140 (MQ=255)
                                                          tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTTTAATTGTTGGGCGGTCGGTTGCGGATTAGTAAAGGTGTGGTGGGATAACAgcg                                                                                 >  2:201211/1‑140 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTGTTGT‑GTTGTGGCGCGGGGGGGTGCGGATGGGGAAAGGGGGCGGGGCATAACAGcgcg                                                                               >  3:349264/1‑140 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTTCTTCGGGCGGGGTGGGATGCCGATGAGTAAAAGTGGCGTGGCAAAACAccgag                                                                               >  4:405479/1‑138 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTTCTTATTGCGCGGTCGGTTGAGGATGAGTTAAGGTGTAGTGGGCTAACAGcgcg                                                                               >  1:82137/1‑140 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACGTATGGCGCGGTCGGTTGCGGATGAGTAAAGGGTTCGTGGGATAACAGcgcg                                                                               >  2:509599/1‑140 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTCTGGCGCGGTCGGGTGCGGATGAGGAAAGGTGTGGTGGCATATCAGagcg                                                                               >  2:278064/1‑140 (MQ=255)
                                                             cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                              <  2:405227/140‑1 (MQ=255)
                                                             cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGATTTTAGTGAAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGGTGCGGAGGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                              <  1:220624/140‑1 (MQ=255)
                                                              ggCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGcgcg                                                                               >  8:4800/1‑138 (MQ=255)
                                                              ggCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGc                                                                             <  4:11769/140‑1 (MQ=255)
                                                                 gACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTATGGCGCGGTCGGTTGCGGATGTGTTAAGGTGTGGTGGCATAACAGCGCGGCAac                                                                          >  2:375347/1‑140 (MQ=255)
                                                                 gACGCTGAAACTCTTGCCGGGATAGATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGGCGGGTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCa                                                                            <  7:641285/138‑1 (MQ=255)
                                                                  aCGCTGAAACTCTAGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTTCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                         <  1:132547/140‑1 (MQ=255)
                                                                       gAAACTATTGCCGGGATATATCACTCATACCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGA‑TT‑TTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGAc                                                                    <  6:652223/140‑1 (MQ=255)
                                                                              ttGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTTCTTATGGGGCGGTCCGTTGCGGATGTGTTAATGTGTCGTGGCATAAACCCGCGGCAACACCGACGTaaacg                                                             >  5:391720/1‑136 (MQ=255)
                                                                                gCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTTCTTCTGGCGCGGGCGGTTGCGGATGAGTAAATGTGTCGTGGCATAACACCGCGGCCACACCGACGTAATCgat                                                           >  3:341913/1‑140 (MQ=255)
                                                                                   gggCTATATCACCCAAAACCCCAACCTTACAATTTTTTTTTTTATGGTAGTCTCCGG‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                        <  1:368382/140‑1 (MQ=255)
                                                                                   gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCTTCAACACGGACGTACTCgatgat                                                        <  5:671676/140‑1 (MQ=255)
                                                                                   gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                        <  6:99163/140‑1 (MQ=255)
                                                                                   gggATATAGCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                        <  4:58470/140‑1 (MQ=255)
                                                                                              cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGGACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                             <  2:411072/140‑1 (MQ=255)
                                                                                               tCATATCACTCTCCTTAGATTTTTTGTTTTTAGTGTAGAGTCGGT‑TG‑TTGTTCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                            <  3:235856/140‑1 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                            <  6:409208/140‑1 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                            <  1:509599/140‑1 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTATGTCGCGGGCGGTTGGGGGTGTGGTAAGGTGGCGTGGCATAACAGCGCGGGACCACGGCCGTACTGGATGAGCTCTTCAAGCgg                                            >  2:230052/1‑139 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTATTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGGGTCGTGGCAAAACAGCGCGGCAACACGGACCGACACGATGATCTCATCaaaccc                                            >  4:295179/1‑136 (MQ=255)
                                                                                                        tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAATGTGGCGTGGCATAACAGCGCGGCAACCCGGCCGTACTCGATGATCTATTCAAGCGACAATTCAAg                                   >  4:504725/1‑140 (MQ=255)
                                                                                                         ctcCTTACCTTTTTTGTTTTTCGGGTAGAGGCGGG‑TT‑TTGTACTGCTGGCGCGGTCGGTTGCGGATGAGTACAGGTGGCGGGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACTTTTCAAGc                                  <  2:193430/140‑1 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTACTTATTGCGCGGGCGGTTGAGGATGAGTAAATGGGTCGTGGCATAACAGCGCGGGCAAACCGAAGTACTCGATGATATCTTAAATCGACATTTaaatc                                  >  4:160080/1‑138 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTAATTATAGCGCGGTGGGTTGCTGAAGATAAAATGTGTGGTGGCCTAACAGCGCGGCCACAACGACCTTAACGATGATCTAATGAAGCCACATTTaaatc                                  >  6:93399/1‑138 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                  <  2:151490/140‑1 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑GT‑TTTTTCGTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCCCGGCCACACGGCCGTACTCGATTCTCTCTTACAGCGCCAATTCAAGc                                  >  6:671934/1‑140 (MQ=255)
                                                                                                          tacTTACATTTATTGTTTTTAGTGTAGAGTCTGT‑GT‑ATGTACTTCAGGCGCGGTCGGTTGCGGATGAGTAAAGGGGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTAAAGCGACATTTCAAGct                                 <  3:681374/138‑1 (MQ=255)
                                                                                                           ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTAGTTCTGGCGGGGTCGGTTGCGGATGAGTAAATGTGTAGTGGGATAACAGCGCGGCAACACGGACGTACTCGATGATATCTTAAAGCGCCATTTAAAgg                                  >  8:306525/1‑137 (MQ=255)
                                                                                                           ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGGGCGGTGGGTTGCGGATTAGTTAAGGTGTCGTGGCATAAACGCGCGGCAACACGGACGTACTAGAAGATCTATTAAAGCGACAATTaaaccaa                                >  2:687518/1‑136 (MQ=255)
                                                                                                            cTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACATATGGCGCCGTCGGTTGCGGAAGAGTAAAGGAGTCGGGGGATAACACCCCGGCAACACCGACGGACTAGAACATCAAATGAAGCGACATTTAAAggg                                 >  8:612662/1‑136 (MQ=255)
                                                                                                               aCATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                            <  3:413802/140‑1 (MQ=255)
                                                                                                               aCATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTAATTAAGGCGCTCTCGGTTGCGGAAGAGTTAAGGTGTCGTGGCAAATCACAGCCGCCACACGGACGTAGACGATGATCTCTTCAACCCACATTTAAAGatcgtac                            >  6:179905/1‑136 (MQ=255)
                                                                                                                            ttAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt               <  1:538002/140‑1 (MQ=255)
                                                                                                                            ttAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt               <  1:674588/140‑1 (MQ=255)
                                                                                                                               ttgtAGAGTCGGT‑TT‑TTGTTCTTCTGGCGCGGTCGGTTGCGGATTAGTAAAGGAGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt            <  5:426567/139‑1 (MQ=255)
                                                                                                                                 gtAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt            <  7:228487/138‑1 (MQ=255)
                                                                                                                                 gtAGAGTCGGT‑TT‑TTGTACTGCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt            <  7:613535/138‑1 (MQ=255)
                                                                                                                                     agTCGGG‑TT‑TTGGACATATGGGGCGGTGGGTTGCGGATGAGGAAAGGTGTCGTGGCATAACAGCGCGGCAAAAAGGCCGGACTCGATGAACACTTCAAGCGCAATTTAAAGCAGCTCCGGATCTACGTCATAGTGGTAAc      >  4:665085/1‑140 (MQ=255)
                                                                                                                                         ggT‑TT‑TTGTACTTCTCGAGCGGTCGGTTTCGGATGAGTAAAGGTGTCGTGGCATAACAGATCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa  <  3:489596/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAA  >  NZ_CP022686/1674085‑1674361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A