Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F2 I0 R1 181 7914.3 8336376 98.4% 8202993 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,224 T→G 17.7% K188N (AAA→AAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2240TG17.7% 229.5 / 15.9 150K188N (AAA→AAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (31/89);  new base G (28/2);  total (59/91)
Fisher's exact test for biased strand distribution p-value = 7.20e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

TTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAA  >  NZ_CP022686/1674085‑1674361
                                                                                                                                           |                                                                                                                                          
ttCTTGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGt                                                                                                                                            <  5:588493/140‑1 (MQ=255)
ttCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGTCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGt                                                                                                                                            <  8:365346/140‑1 (MQ=255)
ttCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGt                                                                                                                                            <  2:827058/140‑1 (MQ=255)
  cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGCTATATCACTCATAACACTCTCCTTACATTTTTTTGTTTTAGTGTAGAGTCGGg‑tt                                                                                                                                         >  3:712832/1‑140 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGCCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTg                                                                                                                                      >  4:726943/1‑140 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGt‑ttttt                                                                                                                                      >  6:648729/1‑139 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGg‑ttttt                                                                                                                                      >  7:501547/1‑139 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATGTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTg                                                                                                                                      <  5:913462/140‑1 (MQ=255)
          gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGCAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACtt                                                                                                                                 >  2:475008/1‑140 (MQ=255)
          gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTAACAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGttaat                                                                                                                                 >  6:520647/1‑136 (MQ=255)
           caAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCGGAAACTGTTGCCGGGATATATCACTCATAACACTCTCCGTACATTTTTTGTTTTTAGGGGAGAGTCGGG‑GTTTTTACttg                                                                                                                                >  6:1766/1‑139 (MQ=255)
             aacctCAGCGCGGCGCTGGCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTATTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTg                                                                                                                              <  6:170508/136‑1 (MQ=255)
                    gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGTCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCATTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGcggt                                                                                                                       <  5:863298/140‑1 (MQ=255)
                    gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGcggt                                                                                                                       <  6:290031/140‑1 (MQ=255)
                     cgcgGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACTCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGcggtc                                                                                                                      <  7:509723/140‑1 (MQ=255)
                     cgcgGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGcggtc                                                                                                                      <  3:313486/140‑1 (MQ=255)
                     cgcgGCGCTGCCGGGTTGCAGTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTGGTAATTCTGGCGcgggg                                                                                                                      >  1:391145/1‑138 (MQ=255)
                          cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTTTACTTATGTCGAGGGCGGTTg                                                                                                                 >  1:254162/1‑140 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCATGGCCTGACGGCGACGCTGAAACTCTTGCCGTGATATATCACTCATAACACTCTCCTTACATATTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  6:673481/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGATGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  2:296285/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTTAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  5:618695/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  6:838163/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  1:557096/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  3:621172/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCAACCAGGGCCTGACGTCGACGATGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  7:27071/140‑1 (MQ=255)
                             ggCCGGGTTGCATTTGCTCTACCAGGGGCTGACGGCGACGCTGAAACTCTTGCCGGGATTTATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  8:678382/139‑1 (MQ=255)
                             ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                              <  5:772451/139‑1 (MQ=255)
                               ccGGGTTGCATTTGCTCTTCCAGGGCCTGACGGCGACGCTGTAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                            <  5:513400/140‑1 (MQ=255)
                                cGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCCGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATg                                                                                                           <  2:916293/140‑1 (MQ=255)
                                    ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGATGCGGG‑TGTTGTACTTATGTCTCGGTCGGTTGCGGATGattt                                                                                                       >  3:600774/1‑137 (MQ=255)
                                       cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAg                                                                                                    <  1:460015/140‑1 (MQ=255)
                                         tttGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTTTTATTCTTGGGCGGGGGGGTGCGGGATAGTAAAAgt                                                                                                  >  3:516019/1‑140 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  1:395794/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  4:700376/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                <  8:837590/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATAACTCATATCACTCTGCATACATTTTTTGTTTTTAGTGTAGAGGCGGT‑TTTTGTACTTCTGGCTCGGTCGGTTGCGGATGAGTAAAGgtgg                                                                                                <  2:19204/140‑2 (MQ=255)
                                            gCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACCCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTATGTCGCGGTCGTTTGCGGATGAGTTAATgtttg                                                                                               >  6:231285/1‑137 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATACCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                              <  7:641979/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                              <  3:347650/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                              <  1:855841/140‑1 (MQ=255)
                                                aaCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGTCGCTGTCGGTTGCGGATGAGTAAAGGTGTCGTgg                                                                                           <  4:736701/139‑1 (MQ=255)
                                                       gCCTGACGGCGCCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCGTACATTTTTTGTTTTTAGTGTAGAGTCGGTGTTTTGT‑CGTGTGGGCCGGGCGGGGGAGGATGAGGAAACGTGTCGAGGccgaacc                                                                                    >  5:342753/1‑134 (MQ=255)
                                                       gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACGTGGGGCGCGGTCGGTTGCGGAGGAGTAAATGGGTCGTgggaatacc                                                                                    >  4:612655/1‑133 (MQ=255)
                                                        ccttcaggCGACTCTGAAACTAGTGGCGGGATTTTTCACTCTTTAATATCTACTTTCATTTTTTTTTTTTAGTGTAGAGTCGGT‑TTTTGTAGTTCTGGCGCTGTAGGTTGCGGAGGAGTAAAGGTGTCGTTGCATAACAg                                                                                   <  4:901783/134‑1 (MQ=255)
                                                        cccgACGGCGACGCTGAAACTCTTTCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                   <  4:375955/137‑1 (MQ=255)
                                                        ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                   <  6:563497/140‑1 (MQ=255)
                                                        caggACTGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACGTCTGGCGCGGTCTGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                   <  4:966791/137‑1 (MQ=255)
                                                          tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTATGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCGATACAgcg                                                                                 >  4:319537/1‑140 (MQ=255)
                                                          tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTTTTAATCTGGGGCGGGCGGTTGGGGATGTGGTAAGGTGTGGTGGCGTAACAgcg                                                                                 >  7:700098/1‑140 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACAGTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTGGTTAGTATGGCGGGGTGGGTTGCGGGTTAGTTAATGTGTGGTGGGCTTACAGcgcg                                                                               >  3:703482/1‑140 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTTTAATTCTGGCGCGGTGGGGTGGGGGTGAGTAAATTTGTAGTGGCATAACAGcgcg                                                                               >  6:276389/1‑140 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTGTACTTCTGGGGCGGTGGGGGGGGGGTGATGTAAGGTGGAGGGGGGTAACAGcgcg                                                                               >  1:705100/1‑140 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTGTAAGTCTGGGGCGGTGGGGTTCGGTTGAGGTAAGGTGTGGGGTCTTAACcgcgcg                                                                               >  4:399655/1‑140 (MQ=255)
                                                             cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                              <  7:576699/140‑1 (MQ=255)
                                                                cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACCGCGCGGCaa                                                                           >  2:749019/1‑140 (MQ=255)
                                                                 gACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTTTACTTGTTGCGCGGGCGGGTGGGGATGAGTAAAGGTGTGGGGGGCTAACAGCGCGGCAac                                                                          >  8:472211/1‑140 (MQ=255)
                                                                  aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                         <  1:64059/140‑1 (MQ=255)
                                                                  aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGGGGCATAACAGCGCGGCAaca                                                                         <  6:442596/140‑1 (MQ=255)
                                                                      tGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGa                                                                     <  8:585709/140‑1 (MQ=255)
                                                                            tctTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACt                                                               <  1:973985/140‑1 (MQ=255)
                                                                              ttGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTGTACTTATGTGGCCGTCGGATGCGGATGAGGAAGGGTGTGGGGGCATTACACCGCGGGCACCCGGCCGGACTCg                                                             >  4:1059614/1‑140 (MQ=255)
                                                                                   gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                        <  2:248586/140‑1 (MQ=255)
                                                                                   gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                        <  3:99741/140‑1 (MQ=255)
                                                                                   gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGGGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                        <  2:519523/140‑1 (MQ=255)
                                                                                   gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGAGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                        <  7:940404/140‑1 (MQ=255)
                                                                                        atatCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTGTGATTCAGGGGCGGGGGGGGGGGGGTGAGGAAAGTTGGTGTGGCCGTACCGCGCGGCCACCCGGACGGACGCGATGAtctcat                                                   >  5:69972/1‑138 (MQ=255)
                                                                                              cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                             <  7:811570/140‑1 (MQ=255)
                                                                                                 aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTGTTAATCTGGCGCGGTCGGTTTCGGATGAGTAAAGGGGGGGTGGGATTAAAGAGGGGCAACCCGGACGGACTATATGATCAATTCAATCGACa                                          >  6:558839/1‑140 (MQ=255)
                                                                                                 aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTGTACTTAAGGCGCGGTCGGTTGCGGATGACTAAAGGTTTAGTGGCCTAACAGCGCGGCAACCCGGACCTACGCGAGGATCTCTTCAAGCGCCa                                          >  6:774331/1‑140 (MQ=255)
                                                                                                 aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTAGGG‑TTTTGTACATGAGGCGCGGGAGGTTTCTGATGTCTAAAAGTGTCGTGGCAGAAAAGCGCGGCAAAAACGAAGTACTCTATGATATCATCAAGcaaaa                                          >  6:948275/1‑136 (MQ=255)
                                                                                                      acTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                                     <  5:210552/140‑1 (MQ=255)
                                                                                                        tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                   <  5:942247/140‑1 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTTTTCTTCTGGCGCGGTCGGTTGCGGATGAGTTAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTGCTCGATGATCTCTTCAAGCGACATTTaaacc                                  >  1:1005472/1‑138 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTTTTCTTATGGCGCGGTCGGTTTCGGATGAGTAAAAGTGTGGTGGCATAACATCCCGGCAACACCGCCGTAATAGATGATCTATTAAATCGACAATTaaatc                                  >  4:317081/1‑138 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTTTACTTCTGGCGCGGTCGGGTGCGGATGTGTATAGGTGTGGTGGGATAACAGAGCGGCAACACGGACGTACTCGAAGATGACTTCCAGCGACAtatacagc                                  >  8:394480/1‑133 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTTTACTTATTGCGCGGGCGGTTGGGGATGAGTAAATGTGTAGTGGCATAACAACCCGGCAACACGGACGTAATCGATGATATCTTAAAGCGACAATTCAAGc                                  >  4:517712/1‑140 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTTTACTTATGGCGGGGTCGGGTGCGGTTGTGTAAATGTTTCGTTGGATATAACGGCGGCAACACCGCCGTACTCGCATATATCTTCAATCGAAATTTCaacc                                  >  6:958909/1‑138 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTTTAATTATGGCGCGGTCGGTTTCGGATGAGGAAAGGTTTAGTGTCATAAAAGCGCGGCAAAACGGACGTACTCGATTATATCTTCAAGCGAAATTTAAAga                                  >  4:16756/1‑139 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                  <  1:1072349/140‑1 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                  <  3:744476/140‑1 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTAAGGCGCGGGCGGTTGCGGAATAGTAAAGGTGTGGTGGCATAACACCGCGGCCACACCGACGTACACGAAGATCACCTCAAGCGACCATTCAAGc                                  >  8:652899/1‑140 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTAATTAGGTGGCGGTCGGTTGCGGATTAGTAAAGGTGTAGTGGCATAACCACCCGGCAACACGGACGTACTCGATGATAACTGAAAGCCACAtttacagc                                  >  8:740644/1‑135 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTTTTAATCTGGGGCGGTGGGTTTCGGATGAGTAAATGTGTGGTGGCATAACAGCGCGGCCACACGGGCGTAATCCATGATCTCTTCAATCGACAattaatcc                                  >  4:520727/1‑135 (MQ=255)
                                                                                                         ctcATTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                  <  7:751751/140‑1 (MQ=255)
                                                                                                          tcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                              <  7:281156/79‑1 (MQ=255)
                                                                                                          tcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCg                                                                                              >  8:281156/1‑79 (MQ=255)
                                                                                                            cTTACATTTTTTGTTTTTAGAGTAGAGTCGGG‑TTTTGTACTTAATGCGCGGTGGGTTGAGGATAATTTAACTTTTAGCTGAATAAAAACGCCGCAACACCGACCAACTCGTTTATATATTAAAGCGACAATTaaaagtct                               >  4:844590/1‑135 (MQ=255)
                                                                                                               aCATTTTTTGTTTTTAGTGTAGAGTCGGG‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                            <  8:908696/140‑1 (MQ=255)
                                                                                                                    ttttGTTTTTAGTGTAGAGTCGGT‑TTTTTTACTTATGGCGGGGGCGGGTTGGGTTTAGTAAATGTTTGGTGGGCTAAAAGCGCGGCAAAACGGACGTAATCGCTGCTCTCGTTCAGCGGCATTTACAGCTGTTGCAGAtg                       >  4:384289/1‑139 (MQ=255)
                                                                                                                      ttGTTTTTAGTGTAGAGTCGGT‑TTTTTTAATGCTGGCTCGGTCGGTTGGGGATGCTTAAAGTTGTGGTTGCATAACATCTCGGCCAAAAGGGAGGACTCCATGATTACTAGAAGCGACAATTAAAGCTGTTCCGGAtctt                     >  6:723139/1‑140 (MQ=255)
                                                                                                                      ttGTTTTTAGTGTAGAGTCGGT‑TTTTGTTGTTATGGCGGGGTGGGTTGGGGGTGAGTTAAGGTGTGGGGGGATAACAGGGCGGCCACAAGGACGTACTCGCTGATAACTTCAAGCGACATTTCAATCTGTGCCTgctatt                     >  8:141363/1‑135 (MQ=255)
                                                                                                                      ttGTTTTTAGTGTAGAGTCGGG‑TTTTGTTCTTAGGGAGCGGTAGGTTGGGGATGAGTAAATGTGTGGTTGCAAAAAAGCGCGGCAACACGGACGTACACGATGATCTCTTCAAGCGCCATTTAAATCTCTTACTGAtatt                     >  8:776028/1‑140 (MQ=255)
                                                                                                                           tttAGTGTAGAGGCGGT‑TGTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtct                <  8:181129/140‑1 (MQ=255)
                                                                                                                               gtgtAGAGTCTGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt            <  3:795887/140‑1 (MQ=255)
                                                                                                                               gtgtAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt            <  4:555306/140‑1 (MQ=255)
                                                                                                                                 gtAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTtcgtcg          <  8:739113/140‑1 (MQ=255)
                                                                                                                                    gagTCGGT‑TTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTaa       <  2:179945/140‑1 (MQ=255)
                                                                                                                                       tCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAAccc    <  8:241864/140‑1 (MQ=255)
                                                                                                                                       tCGGG‑TTTTGTACTTATGGCGCGGTCGGGTGGGGATGAGGAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTAGATGATCTCTTCAAGCGCCATTTCAAGCTCGTGCTGATCTTCGTCTTCGGCGTAAccc    >  4:995116/1‑140 (MQ=255)
                                                                                                                                         ggT‑TTTTGTACTTCTGGCGCTGTCTGTTGCGGTTGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACTGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa  <  8:564460/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAA  >  NZ_CP022686/1674085‑1674361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A