Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A29 F2 I0 R1 207 10827.9 9263290 98.4% 9115077 135.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,224 T→G 12.3% K188N (AAA→AAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2240TG12.3% 311.4 / 14.3 179K188N (AAA→AAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (51/99);  new base G (23/3);  total (74/105)
Fisher's exact test for biased strand distribution p-value = 3.23e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

TTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAAC  >  NZ_CP022686/1674085‑1674362
                                                                                                                                            |                                                                                                                                            
ttCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑Gt                                                                                                                                              <  1:621880/140‑1 (MQ=255)
ttCTGGAGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑Gt                                                                                                                                              <  3:37553/140‑1 (MQ=255)
  cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑Gttt                                                                                                                                            >  1:539592/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑Gttt                                                                                                                                            >  7:49725/1‑138 (MQ=255)
    ggTGCAGCAAAAAACAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCATGACGGCGACGCTGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑Gttttt                                                                                                                                          >  4:892335/1‑140 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑Gtttttt                                                                                                                                         >  4:1062554/1‑139 (MQ=255)
      tGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑Gttttt                                                                                                                                          >  5:971000/1‑138 (MQ=255)
      tGCAGCAAAAATAAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAACATCTTGCCGGGATATATCACTCATAACACTCTCGGTACATTTTTTGTTTTTAGTGTAGAGTCG‑Ggtttt                                                                                                                                          >  6:957172/1‑138 (MQ=255)
        cagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGTATATATCACTCATAACACACTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGTGttttttca                                                                                                                                       >  1:914294/1‑137 (MQ=255)
          gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGtta                                                                                                                                      >  8:125127/1‑136 (MQ=255)
             aaaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCG‑GGTTTTGTAcgtg                                                                                                                                   >  8:337685/1‑135 (MQ=255)
                   agagcggcgCTGTCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGcgg                                                                                                                           <  2:1103849/137‑1 (MQ=255)
                   aGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGcgg                                                                                                                           >  4:961064/1‑140 (MQ=255)
                    gcgcGGCGCTGCCGGGGTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGcggt                                                                                                                          <  3:341975/140‑1 (MQ=255)
                     cgcgGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGcggtg                                                                                                                         >  1:555993/1‑139 (MQ=255)
                          cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTg                                                                                                                    <  1:858104/140‑1 (MQ=255)
                          cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTg                                                                                                                    <  3:162740/140‑1 (MQ=255)
                          cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTg                                                                                                                    <  1:1010950/140‑1 (MQ=255)
                            cTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTAATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GGGTTTGTACTTATGGGGCTGTCGGGTg                                                                                                                    >  6:258011/1‑138 (MQ=255)
                             tGCCGGTTTTCATTTGCTCTTCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTACgg                                                                                                                 <  2:1141045/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGGCGGTTGc                                                                                                                   <  5:1062592/138‑1 (MQ=255)
                             ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCCTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                                   <  6:276196/137‑1 (MQ=255)
                               ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                 <  7:1033010/138‑1 (MQ=255)
                               ccGGGGTGCATTTGCTCTACAAGGGCCTGACGTCGACGCTGAACCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCT‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                 <  6:780637/138‑1 (MQ=255)
                                  gtgtGCTTTTTCTCTACAAGGGCCTGACGTCGACGCTGAAACTCTTGCAGGGATATCTCACTCATTAAACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCG‑GTTTTTGTACTTCTGGCGCGGGCGGTTGCGGATG‑Ag                                                                                                           <  3:318570/137‑1 (MQ=255)
                                    ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTTCTTGGGGCGCGGGCGGTTGCGGTTG‑AGTa                                                                                                         >  3:344498/1‑140 (MQ=255)
                                           tgagctACCAGGGCCTGTCTGCGAGGCTGATAATCTATTGCGGCTATATCACTCTTAACACTCTCCTTTAATATTTTGTTTTTAGTGTTGAGTCG‑GGGTTTGTACTTCTGGCGCGGTCGGGTGCGGATT‑AGATAAGgt                                                                                                    <  8:400762/134‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACTCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgt                                                                                                    <  5:251499/138‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGTAACTCTTGCCGTGATATATCACTCATAACACTCTACTTTCATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                  <  1:871155/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCATGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgt                                                                                                    <  7:290364/138‑1 (MQ=255)
                                             ctctTCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                  <  6:630135/138‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                  <  6:97923/138‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                  <  6:538156/138‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                  <  6:544010/138‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                  <  6:898967/138‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCg                                                                                                <  4:744376/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTTTACTTATTGCGCGGTCGGGTGCGGTTG‑ATTAAATGTGTCg                                                                                                >  4:14420/1‑140 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCg                                                                                                <  1:43416/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGAGATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                  <  6:1008558/138‑1 (MQ=255)
                                             cgctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGgtgt                                                                                                  <  8:68125/136‑1 (MQ=255)
                                                   cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTGTTTGTACTTGTGGGGGGGTCGGTTGGGGGTG‑TGTTAATGTGtggtggg                                                                                            >  5:609021/1‑137 (MQ=255)
                                                      ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTAG‑GTTTTTGTAGATATGGAGAGGGCGGTTGGGGCTG‑AGTAAAGGTGTGGTGGCG‑Taaa                                                                                      >  1:782296/1‑139 (MQ=255)
                                                        ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAg                                                                                    <  2:805425/140‑1 (MQ=255)
                                                          tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACCTTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTGTACTTCGGGCGCGGTCGGTTGCGGGTG‑AGTAAAGGTGTCGTGGCC‑TTACAgcg                                                                                  >  2:963125/1‑140 (MQ=255)
                                                          tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTTTTCAGGTGGCGCGGTCGGGTGGGGTGG‑AGTAAAGGTGTCGGGGAA‑TAACAggc                                                                                  >  2:191112/1‑138 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTTTAATTATGGGGCGGTGGGGTGGGGATG‑AGTAAAGGTTTGGTGGGA‑TAACAGcgcg                                                                                >  1:346837/1‑140 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTTATTGTGGCGCGGGGGGGTGGGGATG‑TGTAAAGGTGTGGTGGGA‑TAACAGcgcg                                                                                >  3:555313/1‑140 (MQ=255)
                                                             gggCGACGCTGAAACTCTTTCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTATTGTCG‑GTTTTTGTACTTCTTGCGCGGGCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAgctc                                                                                 <  5:391190/137‑3 (MQ=255)
                                                              ggCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTTATTATGGCGCGGTCGGTTGAGGATG‑AGTTAAGTTGTAGTGGGA‑TAACAgcgcc                                                                                >  5:951125/1‑137 (MQ=255)
                                                                cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCaa                                                                            <  3:424162/140‑1 (MQ=255)
                                                                  aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCaa                                                                            <  7:162718/138‑1 (MQ=255)
                                                                  aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTAATTCAGGCGCGGTGGGTTGGGGATG‑TGTAAAGGTGTGCTGGCA‑TAACAACGCGGGAacc                                                                          >  1:1187520/1‑139 (MQ=255)
                                                                       gAAACTCTTGCAGGGATATATCACTCATAACACTCTCCTTTCATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGTTG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGAc                                                                     <  1:1070836/140‑1 (MQ=255)
                                                                         aaCTCTTTCCGGTATATATCACTCCTCACACTCGCCTTACATTTTTTGTTGTTAGTGTAGAGTCT‑GATTTTGAACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAATGGGGTCGTGGCA‑TAACAGCGCGGCAACACGGACGt                                                                   <  4:850316/140‑1 (MQ=255)
                                                                         aaCTCTTGCCGTGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGGCGTGGCA‑TAACAGCGCGGCAACACGGACGt                                                                   <  2:503386/140‑1 (MQ=255)
                                                                         aaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTATAGTCG‑GTTTTTGTACTTCTGGCGAGGTCGGTTGCGGATG‑AGTAAAGGTGTCTTGGCA‑TAACAGCGCGGCAACACGGAc                                                                     <  6:333091/138‑1 (MQ=255)
                                                                               tGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTTTACTTCTGGCGCGGGGGGTTGGGGTTGTAGT‑AAGGTGTAGTGGCG‑TAACAGCGCGGAAACACGGCCGGAcgcgc                                                             >  3:246163/1‑139 (MQ=255)
                                                                                   gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCgatgat                                                         <  4:327017/140‑1 (MQ=255)
                                                                                        atatCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTTTAATTCTGGCGCGGTCGGTTTCGGATG‑TGTTAAGGTGTCGTGGCA‑TAACAGCCCGGCAACACGGACGTACTCGAGGAtctc                                                      >  8:341847/1‑138 (MQ=255)
                                                                                            cACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTTTTGCGCGGTCGGGTTCGGATT‑AGGAAAGTTGTGGTGTCA‑TAACAGAGCCTCCTCACGGACGTACTAGATTATATCttaa                                                  >  6:445257/1‑136 (MQ=255)
                                                                                               tCATAACAGTCTCCTTTCATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGGTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                             <  2:777317/140‑1 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTTATTCTGGCGCGGTGGGGTTGGGATG‑AGTAAAGGTGGGGGGGGG‑TAACAGCGCGGCAAAACGGACGTACTCGATGATAACGTCAATCGa                                             >  4:732516/1‑140 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGc                                               <  5:676274/138‑1 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGc                                               <  8:1058104/138‑1 (MQ=255)
                                                                                                cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACATATGGCGAGGTCGGGTGCGGATG‑AGGAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTAATAGATGATCTATTAAAGCg                                              >  8:211643/1‑138 (MQ=255)
                                                                                                 aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTATGGCGCGGTCGGTTGCGGATG‑AGTAAATGTGGGGTGGGC‑TAACAGCGCGGCAACACGGGCGTACTCGATGATCGCGTCAAGCGACa                                           >  4:933244/1‑140 (MQ=255)
                                                                                                   aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTTTTATTCTGGCGCGGGAGGTTTCGGCTG‑AGTTAAGGTGTCGGGGCA‑TAACCGCGCGGCAACAAGGGCGTACTAGATGATCGCGTAAAGCGACAtt                                         >  3:866786/1‑140 (MQ=255)
                                                                                                   aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTTTACTTCTTGCGCGGTAGGGTGCGGATG‑AGTAAAGGTGGAGTGGCA‑TAACATCGCGGCAACCCGGACGTACTAGATGATCTCTTACAGCGACa                                           >  7:325467/1‑138 (MQ=255)
                                                                                                   aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTTTAAGTAATGGGCCGTCGGTTGGGGATG‑AGTAAAGGTGTCGTGGCA‑TAAAAGAGCGGCAACACGGCCGTTCTAGCTGATATCTTCAAACGCCa                                           >  7:789423/1‑138 (MQ=255)
                                                                                                   aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTGTACTTATGGGGCGGTGGGTTGCGGATG‑ATTAAATGTGTGCTGGCA‑TAACACCGCGGCCACCCGGCCGGACGAGATGATCTCTTCAAGCCACAtt                                         >  4:336946/1‑140 (MQ=255)
                                                                                                    acacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                        <  2:583968/140‑1 (MQ=255)
                                                                                                     cacTCTCCTTACATTTTTTGTTTTTAGTGGAGAGGCG‑GGTTTTGGACTTCAGGCGCGGTCGGGTGCGGTTG‑AGGTAAAGTGTCGTGGCA‑TAAAAGCCCCGCCACCCGGCCGGACGCGATGTTCTCTTCAACCGCCAtt                                         >  6:712102/1‑138 (MQ=255)
                                                                                                        tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTTTAATTATGGCGCGGTCGGTTGCGGATG‑AGTAAAAGTGTCGTGGCA‑TAAAAGCGCGGCAACACTGACGTACTCGAAGATCTCTTAAAGCGACATTTaaac                                    >  4:684655/1‑139 (MQ=255)
                                                                                                        tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGGGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                                      <  5:699682/138‑1 (MQ=255)
                                                                                                        tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GATTTTGTACTTCTGGCTCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                    <  3:76681/140‑1 (MQ=255)
                                                                                                        tctcCTTACATTTTTTGTTATTAGTGTAGAGTCG‑GTTTTTGTAAGTATGTTGACGGGGGTTTGGGTTG‑AGTAAACGTGTGGTGGCC‑TAACAGAGCCGGAACACCCACCTTCGAGATTATATCTTCAAGCAACAATTaaaa                                    >  2:51092/1‑139 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTTTACATATGGCGCGGTGGGTTTGGGTTG‑AGTAAAGGTTTCGGGGGA‑TAAAAACGCGGCTACAAGGACATACTCGATGATCGCTTGAATCGACATATAAAGc                                   >  4:38497/1‑140 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                   <  2:841021/140‑1 (MQ=255)
                                                                                                         ctcCTTACAGTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTAAATCGGGCTCGGTCGGTTGCTGATG‑TGTAAAAGTGTCGAGGCC‑TAACACCGCGCCCACCCGCACGCACACCACTATCACATCAATCGACAattacatc                                   >  2:863576/1‑135 (MQ=255)
                                                                                                          tacTTCCATTTTTTGTTTTTAGTGTAGAGTCT‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACATAGCTGCAACACGGACGTACTCGATGATCTATTCAAGCGACATTTctat                                    <  5:852882/136‑4 (MQ=255)
                                                                                                           ccTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTTTTCTTCTTGGGCGGTGGGTTGCGGATG‑AGTAAAAGTGTAGTGGCA‑TAAAAGCCCGGCCACAAGGACGGACACGATGATCTATTCAAGCGACATTTAAAGc                                   >  5:219302/1‑138 (MQ=255)
                                                                                                           ccTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTTTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAATGTGTCGTGGCA‑TAACAGCGCGGCAACACGGCCGTAAGAGATTATCTATTCAAGCGACATTTAAAGc                                   >  6:676274/1‑138 (MQ=255)
                                                                                                           ccTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTTTACATATGGCGCGGTCGGTTGAGGATG‑ATTAAAAGTTTAGTGGCA‑TAAAAGCACGGCAAAACGGACGTAATCGATGATATCTTCAAGCCCCATTTCAAGc                                   >  7:1100683/1‑138 (MQ=255)
                                                                                                           ccTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTGTACTTCTGGCGCGGGCGGTTTCGGATG‑AGTAAAGGTGTAGTGGCA‑TAACAGCGCGGCAACACGGACGGACTCGATGATATCTTCAAGCGCCATTTAAAGc                                   >  8:526699/1‑138 (MQ=255)
                                                                                                           ccTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTGTAATTCTGGCGCGGTGGGTTGCGGATG‑AGTTAAGGTGTCGTGGCA‑TAACAGCGCGGCACCACGGACGTACTCGATGCTATCGTCAAGCGACATTTCAAGc                                   >  7:533204/1‑138 (MQ=255)
                                                                                                             ttACATTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTTTAATTATGGGGCGGGGGGTTGGGGAGT‑AGGTAAAGTGGCGTGGGATTTACA‑CGGGGCGCCACGGCCGGACAAGATGATCTCTTCAAGGGTCCTATCAAtctc                                 >  6:1090952/1‑138 (MQ=255)
                                                                                                               aCATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCtt                               <  8:169588/138‑1 (MQ=255)
                                                                                                               aCATTTTTTGTTTTTAGTGTAGAGTCG‑GGTTTTGTTCTTGAGGCGCGGGGGGGTGCGTCGG‑AGTAAAGGGGTAGTGGCA‑GAACAGCGCGGCCACACGGCCCATCGGCATGATATCTTCAAGAGCCAATTAAAGcgattac                             >  4:1133498/1‑134 (MQ=255)
                                                                                                                    ttttGTTTTTAGTGTAGAGTCG‑GGTTTATTTCTTCTGGCGCGGTCGGTTGCGGAAG‑ATTAAATATGTCGTGGCA‑TAAAAGCGCGGCACCACCGACCTACGCGAATCTCTCTTAAAACGACAATTTAAGCTCTTGCttt                          >  6:955592/1‑136 (MQ=255)
                                                                                                                      ttGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtc                        <  5:541753/138‑1 (MQ=255)
                                                                                                                      ttGTTTTTAGTGTAGAGTCG‑GGTTTTGTACATATGGGGCGGTCGGTTGCGGATG‑AGGTAAGGTGTCGTGGCA‑TAACCGCGCGGCCACACGGACGTACACGATGATCTCATACAGCGACATTTAAAGCTATTCCGGaaa                        >  8:637974/1‑136 (MQ=255)
                                                                                                                        gTTTTTAGTGTAGAGTCG‑GGTTTTGTACTTATGGGGCGGTCGGTTGGGGATG‑AGGAAAGGTGTGGTGGCA‑TAACAGCGCGGCCACACGGGCGTACTCGATGATCTATTCAAGCGACATTTAAAGCTCTTGCTGAtggt                      >  6:131697/1‑135 (MQ=255)
                                                                                                                                 gtAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt             <  7:402946/138‑1 (MQ=255)
                                                                                                                                 gtAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTtcgtcg           <  2:555993/140‑1 (MQ=255)
                                                                                                                                 gtAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGGTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt             <  7:432271/138‑1 (MQ=255)
                                                                                                                                  tAGAGTCG‑GATTTTGTACTTGTGGCGCGGGCGGGTGCGTTTG‑AGTCAAGGTGTCGTGGCA‑TAACAGCGATGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTcgtcgt          <  2:1168716/140‑1 (MQ=255)
                                                                                                                                     agTCG‑GGGTTTGTTCTTGTGGGGCGGTAGGTTGGGGATG‑AGGTAAGGGGTCGTGGGA‑TAACAGCGCGGCAACACGGACGGACTAGATGATCTCTTAAAGCGACATTTAAAGCTATTACGGATATACCTCTTAGTAGTAAc       >  4:597762/1‑140 (MQ=255)
                                                                                                                                       tCG‑GTTTTTTTACTTCTGGCGCGGGCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGCCGTACTCGATGATCTCTTCAAGCGACAATTCAAGCTCATCCTGATCTTACTCTTCGTCGTAAccc     >  4:543115/1‑140 (MQ=255)
                                                                                                                                         g‑gTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCACGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa   <  2:105605/140‑1 (MQ=255)
                                                                                                                                         g‑gTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTAGTCGTAACCCaa   <  1:766609/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCG‑GTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATG‑AGTAAAGGTGTCGTGGCA‑TAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAAC  >  NZ_CP022686/1674085‑1674362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A