Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F35 I0 R1 174 9808.2 8488800 98.4% 8352979 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,224 T→G 15.7% K188N (AAA→AAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2240TG15.7% 277.4 / 13.4 164K188N (AAA→AAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (31/102);  new base G (28/2);  total (59/105)
Fisher's exact test for biased strand distribution p-value = 5.95e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

TTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACT  >  NZ_CP022686/1674085‑1674363
                                                                                                                                            |                                                                                                                                            
ttCTTGTGCAGCAAAAAGGAGCGCGGCGCTGACGGGTTGCATTTTCTATACCAGGGCCTGACGTCGAAGCTGAAACTCTTGCCGGGATATATCTCTCATAACACTCTCTTTTCATTTTTTGTTTTTAGTTTTGAGTCGG‑t                                                                                                                                              <  6:61269/140‑1 (MQ=255)
ttCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑t                                                                                                                                              <  6:654557/140‑1 (MQ=255)
  cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑ttt                                                                                                                                            >  8:460989/1‑140 (MQ=255)
    ggTGCAGCAAAAATCATCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑ttttt                                                                                                                                          >  6:951880/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGCCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTAACATTTTTTGTTTTTAGTGTAGATTAGG‑tattt                                                                                                                                          >  6:186050/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑ttttt                                                                                                                                          >  3:1024975/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑ttttt                                                                                                                                          >  7:774792/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑tgttt                                                                                                                                          >  5:31682/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑gtttt                                                                                                                                          >  5:974989/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑gtttt                                                                                                                                          >  8:227125/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTGTATTGTAGAGTCGG‑ttttt                                                                                                                                          >  4:958841/1‑140 (MQ=255)
    ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCGTACATTTTTTGTGTTTAGTGTAGAGTCGg‑ttgtt                                                                                                                                          >  6:539069/1‑140 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑tttt                                                                                                                                           <  1:470954/138‑1 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑gttttt                                                                                                                                         >  5:664654/1‑139 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑gttttt                                                                                                                                         >  7:687958/1‑139 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTg                                                                                                                                         <  3:863474/140‑1 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTg                                                                                                                                         <  8:1016846/140‑1 (MQ=255)
     gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTGTTAGTGTAGAGTCGG‑gttttt                                                                                                                                         >  7:7337/1‑139 (MQ=255)
      tGCAGCAAAAATCAGCGCGGCGCTGCCGTGTTGCATTTGCTCTACCAGCGCCTGACTGCGACGCTGAAACTCTTGCAGGGATATATCACTCATAACACTCTCCTTCCATTTTTTGTTTTTAGTGTAGAGTCGG‑tttgt                                                                                                                                          >  1:396055/1‑136 (MQ=255)
      tGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑gtttt                                                                                                                                          >  1:204331/1‑138 (MQ=255)
          gcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTggagtt                                                                                                                                    >  6:643662/1‑135 (MQ=255)
           caAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTc                                                                                                                                   <  4:856924/140‑1 (MQ=255)
           caAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGttattg                                                                                                                                   >  5:1022247/1‑135 (MQ=255)
           caAAAATCAGCGCGGCGATGCCGGGTTGCATTAGCTCTACCAGGGCCTGACGGGGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACt                                                                                                                                     <  2:946927/138‑1 (MQ=255)
            aaaaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACtt                                                                                                                                    >  1:664557/1‑138 (MQ=255)
            aaaaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACtt                                                                                                                                    >  2:584889/1‑138 (MQ=255)
                   aGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGG‑TTTTTGTACTTCTGGCGcgg                                                                                                                           <  6:613469/140‑1 (MQ=255)
                    gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGcggt                                                                                                                          <  8:530268/140‑1 (MQ=255)
                    gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGcgcg                                                                                                                            <  1:505025/138‑1 (MQ=255)
                       cggcgCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGcggtc                                                                                                                         <  1:90913/138‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                                   <  2:650763/138‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                 <  3:774282/140‑1 (MQ=255)
                             tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                 <  5:17361/140‑1 (MQ=255)
                             ggCCGGGTTGCATTTGCTCTACCAGGGCCCGACGGCGACGCTGAAACTCTTGCCGGGAGATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTGCTGGCGCGGTCGGTGGCgg                                                                                                                 <  6:1015617/139‑1 (MQ=255)
                               ccGGGTTGCATTTGATCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACTCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGac                                                                                                               <  6:986875/140‑2 (MQ=255)
                                     tGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGt                                                                                                           <  1:129578/138‑1 (MQ=255)
                                      gCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTcgggtgcgggtgcggt                                                                                                          >  2:734407/1‑134 (MQ=255)
                                       cATTTGCTCTCCCAGGGCCGGACGGCGACGCGGACACTCGTGCCGGGATCTATCACTCATAACACGCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTCATACTTGCGCGGTCGGTGGCGGATGAGGAAAg                                                                                                       <  8:643388/140‑1 (MQ=255)
                                       cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAg                                                                                                       <  4:927629/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                   <  8:291566/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                   <  5:485931/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                   <  3:37964/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                   <  4:159981/140‑1 (MQ=255)
                                           tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                   <  4:296433/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGTCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                 <  8:574960/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                   <  2:415352/138‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                 <  3:940481/140‑1 (MQ=255)
                                             ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                 <  8:59329/140‑1 (MQ=255)
                                                tACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTGCTTCCATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCTGTCGGTTGCGGATGAGTAAAGGTGTCGt                                                                                                <  2:239209/138‑1 (MQ=255)
                                                aaCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTCCATTTTTTGTTTGTAGTGTAGAGTCGG‑TTGTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTgg                                                                                              <  4:806559/139‑1 (MQ=255)
                                                aaCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCTCGGTCGGTTGCGGATGAGTAAAGGTGTCGTgg                                                                                              <  8:943306/139‑1 (MQ=255)
                                                 aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTTCTTCTGGCGCGGTCGGTTGCGGGTGAGTTAAGGTGTCGTggg                                                                                             >  3:1015980/1‑139 (MQ=255)
                                                 aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGc                                                                                             >  6:515818/1‑140 (MQ=255)
                                                    aGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAATTCTGGCGCGGTCGGTTGCGGATGAGTTAATGTGTCGTGGcg                                                                                            >  1:625104/1‑137 (MQ=255)
                                                      ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTTCTTATGGCGCGGGAGGTGTGGGTTGTGTATAAGTGTCGTGTCaaaaa                                                                                        >  7:612617/1‑139 (MQ=255)
                                                        ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                      <  5:892374/140‑1 (MQ=255)
                                                          tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcg                                                                                    <  4:735033/140‑1 (MQ=255)
                                                          tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGGCGGGGGGGGATGAGTAAAGGTGTCGTGGCATAACAtcg                                                                                    >  5:939754/1‑140 (MQ=255)
                                                          tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTGATGGCGCGGTCGGGGGCGGATGAGTTATGGTGTGGGGGGATAACAggg                                                                                    >  6:579478/1‑138 (MQ=255)
                                                           gccGGCGTCGCTGAAAATCTTGCCGTGATATATCACTAATAACACTCTCCTTACATTATTTGTTTTTAGTGTAGAGTAGG‑ATTTTGTACTTCTGGCGCGGTCGTTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcgc                                                                                   <  6:142904/138‑1 (MQ=255)
                                                            aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTAATTATGGCGAGGTGGGGTGGTGATGAGTATAGGTGTCGTGGGCTATCAGcgcg                                                                                  >  8:713490/1‑140 (MQ=255)
                                                             cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACCCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAATGGTGTCGTGGCATAACAgcgc                                                                                   <  1:958716/138‑1 (MQ=255)
                                                                cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                              <  4:35215/140‑1 (MQ=255)
                                                                 gACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTGCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaaa                                                                             >  6:498542/1‑139 (MQ=255)
                                                                  ccGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGG‑TTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGACATAACAGCGCGGCAaca                                                                            <  3:124747/139‑1 (MQ=255)
                                                                  aCGCTGAAACTCTTGCCGGGATATATGACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGAGG‑TTTTTGTACTGCTGGCGCGGTCGGTTGCGGTTGAGTAAAGGTGTCGTCGCATAACAGCGCGGCAaca                                                                            <  3:1069608/140‑1 (MQ=255)
                                                                  aCGCTGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                            <  7:269747/140‑1 (MQ=255)
                                                                     cTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑Cgg                                                                        <  6:32619/140‑1 (MQ=255)
                                                                      tGAAACTCTTGCCGCGATATATCACTCATAACACCCACCTGACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTGTAATGTTGGC‑CGGGGGGGTGACGGTGGCTAAACGTGTCGTGGCAGTACCGCGCGGCcacagcgc                                                                        >  8:490278/1‑136 (MQ=255)
                                                                        aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGa                                                                       <  1:584889/138‑1 (MQ=255)
                                                                         aaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCTGTCGGTTGCGGATGTGTAAATGTGTCGTGGGATAACAGCGAGGCAACA‑CGGACGt                                                                    <  5:347079/140‑1 (MQ=255)
                                                                          aCTCTTGCCGGGATATATCACTCATAACACTCTCCTTCCATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTa                                                                   <  5:801560/140‑1 (MQ=255)
                                                                          aCTCTTGACGGGAGATCTCACTCTTAACACTCTCATTTCATTTTTTGTTTTTAGTGTAGAGGAGG‑TTTTTGTACTTCTGGGGAGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTa                                                                   <  7:827311/140‑1 (MQ=255)
                                                                            tctTGCCGGGATATATCACTCATAACACTATCCTTACATTATTTGTTTTTAGTGTAGAGGCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTCGCATAACAGCGCGGCAACA‑CGGACGTa                                                                   <  2:124880/138‑1 (MQ=255)
                                                                                 ccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCgatg                                                            <  6:877908/140‑1 (MQ=255)
                                                                                   gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTG‑TTTTTGTACTTCTGGAGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCgatgat                                                          <  3:322859/140‑1 (MQ=255)
                                                                                     gATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGAtct                                                        <  5:178338/140‑1 (MQ=255)
                                                                                              cTCATAACCCTCTCCTTACATTTTTTGTTGTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCg                                               <  8:1005320/140‑1 (MQ=255)
                                                                                              cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCg                                               <  6:696935/140‑1 (MQ=255)
                                                                                              cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAATTATTGCGAGGTCGGTTGAGGATGAGTTAAAGTGTAGTGGGATAACAGCGCGGCAACA‑AGGACGTACTCGATTATATGTTCAAGCg                                               >  8:885983/1‑140 (MQ=255)
                                                                                              cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTGCTTATGGGGCGGTGGGGTGGGGATGAGTAAAGGTGTCGTGGCATAACCGCGCGGCAACA‑CGGCCGTTCTAGCTGATGTCTTAAAGCg                                               >  7:895093/1‑140 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTATTGGGCGGTCGGTTGGGGATGAGGAAATGTTTAGTGGGAGAACAACGCGGCAACA‑CGGACGTACAAGCTGATAAATTCAAGCGa                                              >  3:927739/1‑140 (MQ=255)
                                                                                               tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTAATTCTGGCGCGGTCGGTTGCGGATGAGTAAATGTGTAGTGGCATAACAGCGCGGCAACA‑CGGCCGTACTCGATGAGGTATTCAATCGa                                              >  4:771441/1‑140 (MQ=255)
                                                                                               acATAACACTCTACTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGAGGATGAGTAAAGGTGTCGTGGCATTACAGCGCGGCAACT‑CGGACGTACTCGATGATCTCTTCAAGCGa                                              <  3:269509/139‑1 (MQ=255)
                                                                                                       ctctCCTAACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                                      <  8:508683/140‑1 (MQ=255)
                                                                                                        tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTGTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                     <  5:1011956/140‑1 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                    <  8:566890/140‑1 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                    <  8:734749/140‑1 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAATTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTGGTGACATAACACCGCGGCAAAA‑CGGAAGTACACAATTATCAATTAAAAAGACATTAAAAGc                                    >  4:1014088/1‑140 (MQ=255)
                                                                                                         ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGTGTAAAGGTTTCGTGGCAAAACACCGCGGCACCA‑CGGACGGACTCGATGATCTCTTCAAGCGCCAATTCAAGc                                    >  7:660496/1‑140 (MQ=255)
                                                                                                           ccTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTTTAATTATGGCGCGGTCGGTTGCGGTTGATTAAAGGTGTCGGGGCATAACAGCGCGGCAACA‑CGGCCGTACTCGATGATCTCTTCAAGCGACAATTCAAGc                                    >  2:862247/1‑138 (MQ=255)
                                                                                                                    ttttGTTTTTAGTGTAGAGTCGG‑GTTTTTTACTTCATGCGCGGTAGGTTTCGGATGTGTAAAGGTGTCGTGGCATAACAGCGCCGCAACA‑CGGACGGTCTCGATGATCTATTGAATCGACCTTTGAAGCTCTTCCTGAta                         >  8:383210/1‑139 (MQ=255)
                                                                                                                      ttGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTGATGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTTGCAAAACAGCGCGG‑AACACCCGACCTACTCCAGGAACTCATAAAGCGACATTTAAAACACTTACAGAtagt                       >  4:600428/1‑137 (MQ=255)
                                                                                                                           tgtAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGTTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGAGCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtct                  <  8:694050/138‑1 (MQ=255)
                                                                                                                            tttgtgtAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt                 <  3:531789/137‑1 (MQ=255)
                                                                                                                               gtgtAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                <  1:1073379/138‑1 (MQ=255)
                                                                                                                               gtgtAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt              <  8:443518/140‑1 (MQ=255)
                                                                                                                                 gtAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt              <  2:977116/138‑1 (MQ=255)
                                                                                                                                    gagTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTaa         <  7:585134/140‑1 (MQ=255)
                                                                                                                                            ttttttTACTTAAGGCGCGGTCGGTTGCGGATGAGTAAATGTGTCGAGGCATAACAGCGCGGCAACA‑CGGACGTACTGGATGATCTCTTCAAGCGACATTTCAAGCTGTTCCTGCTCTTCCTCTTAGTAGTAACCCAACt  >  4:465812/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACT  >  NZ_CP022686/1674085‑1674363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A