breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 483 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,105,4190GA74.8% 708.2 / 211.2 349intergenic (+140/+135)RPPX_13820/mscLferredoxin‑NADP reductase/large‑conductance mechanosensitive channel
*CP0099744,132,2310AC74.0% 440.3 / 163.5 238intergenic (+147/+82)RPPX_18375/RPPX_18380membrane protein/phosphonoacetaldehyde hydrolase
*CP0099743,756,3860TC72.8% 877.2 / inf 553intergenic (+268/+372)RPPX_16775/RPPX_16780hypothetical protein/phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
*CP0099741,155,6340TC72.8% 602.4 / 246.8 366G778G (GGT→GGCRPPX_05115hypothetical protein
*CP0099741,277,6200GC72.6% 443.3 / 189.7 307intergenic (+89/+149)RPPX_05635/RPPX_05640aldehyde dehydrogenase/mechanosensitive ion channel protein MscS
*CP0099743,837,7130C.72.1% 16.2 / 58.6 43intergenic (+264/‑287)RPPX_17095/RPPX_17100proteophosphoglycan precursor/cupin
*CP0099743,105,4080AG71.7% 647.8 / 261.9 352intergenic (+129/+146)RPPX_13820/mscLferredoxin‑NADP reductase/large‑conductance mechanosensitive channel
*CP0099745,598,9670CT70.8% 510.1 / 274.3 358P231P (CCC→CCTRPPX_24920hypothetical protein
*CP0099741,896,1840AG70.6% 2348.1 / inf 3354intergenic (‑3962/‑4635)RPPX_08470/RPPX_08480tryptophan synthase subunit beta/hypothetical protein
*CP0099743,771,8830A.69.9% 326.3 / 281.3 174noncoding (76/76 nt)RPPX_16845tRNA‑Glu
*CP0099743,847,5810TC69.9% 163.9 / 182.3 229intergenic (+94/‑246)RPPX_17150/RPPX_17155tRNA‑dihydrouridine synthase C/heat‑shock protein
*CP009974542,1150CT69.8% 40.2 / 28.9 43intergenic (‑376/+153)argD/RPPX_02300acetylornithine aminotransferase/AraC family transcriptional regulator
*CP009974542,1160AG69.8% 39.7 / 26.0 43intergenic (‑377/+152)argD/RPPX_02300acetylornithine aminotransferase/AraC family transcriptional regulator
*CP0099741,895,7340CG69.5% 569.1 / 284.3 302intergenic (‑3512/‑5085)RPPX_08470/RPPX_08480tryptophan synthase subunit beta/hypothetical protein
*CP0099741,897,4710GC69.3% 3696.9 / inf 4645intergenic (‑5249/‑3348)RPPX_08470/RPPX_08480tryptophan synthase subunit beta/hypothetical protein
*CP0099743,837,7170G.68.9% 30.5 / 74.5 46intergenic (+268/‑283)RPPX_17095/RPPX_17100proteophosphoglycan precursor/cupin
*CP0099745,105,3000AG68.8% ‑4.0 / 22.4 32D184D (GAT→GACRPPX_22650benzene 1,2‑dioxygenase
*CP0099741,897,3510CT68.1% 2784.4 / inf 1828intergenic (‑5129/‑3468)RPPX_08470/RPPX_08480tryptophan synthase subunit beta/hypothetical protein
*CP0099741,897,5910TC68.0% 4093.6 / inf 4604intergenic (‑5369/‑3228)RPPX_08470/RPPX_08480tryptophan synthase subunit beta/hypothetical protein
*CP0099741,897,1240CG67.7% 41.5 / 63.2 65intergenic (‑4902/‑3695)RPPX_08470/RPPX_08480tryptophan synthase subunit beta/hypothetical protein

Marginal new junction evidence (lowest skew 10 of 716 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009974 1895750 =305 (1.120)436 (1.780)
+GCCGGTCAGCACGTC
171/270 0.0 17.6% intergenic (‑3528/‑5069) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
?CP009974 1897573 = 4220 (15.500)intergenic (‑5351/‑3246) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
* ? CP009974 1897055 =23 (0.080)184 (0.830)
+28 bp
98/244 2.7 9.7% intergenic (‑4833/‑3764) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
?CP009974 1897574 = 4194 (15.400)intergenic (‑5352/‑3245) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
* ? CP009974 2287857 =95 (0.350)122 (0.670) 75/200 3.2 76.2% intergenic (‑437/+256) RPPX_10255/RPPX_10260 hypothetical protein/potassium transporter KefB
?CP009974 2288067 = 13 (0.070)intergenic (‑647/+46) RPPX_10255/RPPX_10260 hypothetical protein/potassium transporter KefB
* ? CP009974 = 18966750 (0.000)168 (0.740)
+25 bp
95/250 3.3 100% intergenic (‑4453/‑4144) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
?CP009974 1897988 = NA (NA)intergenic (‑5766/‑2831) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
* ? CP009974 1897988 =NA (NA)174 (0.770)
+25 bp
94/250 3.4 78.3% intergenic (‑5766/‑2831) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
?CP009974 = 1898637 58 (0.210)intergenic (‑6415/‑2182) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
* ? CP009974 = 1511356NA (NA)155 (0.570) 115/300 3.4 41.4% noncoding (2595/2595 nt) ISS12 repeat region
?CP009974 1538760 = 219 (0.800)intergenic (‑152/+12) RPPX_06815/RPPX_06820 bacterioferritin/catalase
* ? CP009974 1277572 =276 (1.010)160 (0.680) 96/258 3.6 39.6% intergenic (+41/+197) RPPX_05635/RPPX_05640 aldehyde dehydrogenase/mechanosensitive ion channel protein MscS
?CP009974 = 1277722 251 (1.070)intergenic (+191/+47) RPPX_05635/RPPX_05640 aldehyde dehydrogenase/mechanosensitive ion channel protein MscS
* ? CP009974 = 2287906101 (0.370)112 (0.620) 72/200 3.7 73.2% intergenic (‑486/+207) RPPX_10255/RPPX_10260 hypothetical protein/potassium transporter KefB
?CP009974 = 2288016 15 (0.080)intergenic (‑596/+97) RPPX_10255/RPPX_10260 hypothetical protein/potassium transporter KefB
* ? CP009974 541996 =NA (NA)164 (0.620)
+ACGCA
108/290 3.7 58.8% intergenic (‑257/+272) argD/RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator
?CP009974 2545982 = 119 (0.440)intergenic (+88/‑177) RPPX_11350/RPPX_11355 hypothetical protein/urocanate hydratase
* ? CP009974 = 1095752273 (1.000)117 (0.650)
+50 bp
68/200 4.3 39.7% intergenic (+179/+17) RPPX_04810/RPPX_04815 flavodoxin/carbon‑nitrogen hydrolase
?CP009974 = 4363596 261 (0.960)intergenic (+252/‑63) RPPX_19410/RPPX_19415 alkyl hydroperoxide reductase/hypothetical protein