breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG38.2% 497.4 / 82.1 284intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123063,938,8870CT37.5% 361.1 / 220.3 264S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123061,944,2880AC33.5% 407.2 / 41.7 206G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,020,6700TG31.7% 281.7 / 11.5 163K195N (AAA→AACglgCglucose‑1‑phosphate adenylyltransferase
*AP0123063,527,3680AC31.7% 495.1 / 72.0 240L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306644,4170GA31.5% 781.6 / inf 537intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP012306800,6750AC28.8% 536.3 / 62.8 264T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306341,3770TG28.3% 235.4 / 18.4 127G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123061,226,5570AG27.8% 386.7 / 97.1 192G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123061,854,8510AC26.2% 679.7 / 75.1 282T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP012306935,0540GT26.0% 276.0 / 42.5 96intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123063,182,8610AC25.4% 278.3 / 15.0 114L198F (TTA→TTCyiaNpredicted transporter
*AP0123063,590,3670AC24.6% 639.0 / 33.3 253T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP0123061,309,9140AC24.1% 302.9 / 12.9 143L23F (TTA→TTClsrCAI2 transporter
*AP012306202,4170TG23.7% 710.6 / 29.3 278V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123062,758,9090TG23.6% 614.6 / 13.4 233G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123061,128,4120TG22.8% 766.9 / 44.2 298V29G (GTG→GGG) yciOconserved protein
*AP0123063,903,7910AG22.3% 653.0 / 57.5 256E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123062,680,3450AC21.9% 489.4 / 32.4 202T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit
*AP0123063,590,3610GA21.6% 679.5 / 99.4 269E28K (GAA→AAA) fabRDNA‑binding transcriptional repressor

Marginal new junction evidence (lowest skew 10 of 144 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 1092512416 (1.340)17 (0.060) 14/258 14.6 5.0% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 289 (1.080)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1092334347 (1.120)11 (0.040) 11/258 15.7 3.6% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 289 (1.080)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 2743199377 (1.220)6 (0.030)
+58 bp
4/182 16.8 2.3% intergenic (+264/+350) yhaC/rnpB predicted protein/RNase P, RNA component precursor RnpB
?AP012306 3838241 = 455 (1.470)intergenic (+44/‑136) rnr/rlmB exoribonuclease R, RNase R/23S rRNA (Gm2251)‑methyltransferase
* ? AP012306 = 2743199377 (1.220)6 (0.030)
+56 bp
4/186 17.0 2.7% intergenic (+264/+350) yhaC/rnpB predicted protein/RNase P, RNA component precursor RnpB
?AP012306 3080522 = 315 (1.020)intergenic (+52/+192) pitA/yhiO phosphate transporter, low‑affinity/predicted universal stress (ethanol tolerance) protein B
* ? AP012306 = 215364NA (NA)10 (0.030) 10/288 17.0 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 3600658NA (NA)28 (0.090) 9/292 17.5 NA noncoding (2805/2904 nt) rrlB 23S ribosomal RNA (rrlB)
?AP012306 = 3642045 NA (NA)noncoding (2790/2904 nt) rrlE 23S ribosomal RNA (rrlE)
* ? AP012306 1097204 =329 (1.060)8 (0.030) 8/290 18.0 2.5% coding (320/909 nt) galU glucose‑1‑phosphate uridylyltransferase
?AP012306 1097223 = 310 (1.030)coding (339/909 nt) galU glucose‑1‑phosphate uridylyltransferase
* ? AP012306 = 2275835NA (NA)7 (0.020) 7/290 18.5 NA noncoding (950/1542 nt) rrsG 16S ribosomal RNA (rrnG)
?AP012306 = 2275847 NA (NA)noncoding (938/1542 nt) rrsG 16S ribosomal RNA (rrnG)
* ? AP012306 3375391 =203 (0.660)8 (0.030) 7/296 18.6 3.8% noncoding (2475/2904 nt) rrlC 23S ribosomal RNA (rrlC)
?AP012306 3469170 = NA (NA)noncoding (2439/2905 nt) rrlA 23S ribosomal RNA (rrlA)
* ? AP012306 217057 =NA (NA)8 (0.030) 7/296 18.6 NA noncoding (2438/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 217096 = NA (NA)noncoding (2477/2905 nt) rrlH 23S ribosomal RNA (rrlH)