breseq  version 0.35.4  revision f352f80f4bc9 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 40 shown, sorted by frequency from high to low)
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,147,133	0	T	G	56.7%	‑5.3 / 11.2	30	intergenic (+187/+30)	ybdG_2/bioP	Miniconductance mechanosensitive channel YbdG/Biotin transporter
*	KT2440	3,440,343	0	A	C	54.5%	‑6.4 / 15.8	44	S18R (AGC→CGC)	CJ019_03266	hypothetical protein
*	KT2440	4,025,810	0	C	A	53.8%	5.5 / 17.0	13	intergenic (+58/‑198)	dmdC_5/CJ019_03801	3‑methylmercaptopropionyl‑CoA dehydrogenase/hypothetical protein
*	KT2440	1,123,238	0	C	A	52.4%	7.3 / 31.0	43	intergenic (‑240/+64)	gcvH_1/CJ019_01062	Glycine cleavage system H protein/hypothetical protein
*	KT2440	3,995,418	0	T	G	52.4%	9.9 / 21.0	21	intergenic (+90/‑44)	dmoA_3/allE	Dimethyl‑sulfide monooxygenase/(S)‑ureidoglycine aminohydrolase
*	KT2440	1,542,102	0	T	G	48.1%	8.5 / 10.8	27	intergenic (+31/‑214)	CJ019_01454/groS	hypothetical protein/10 kDa chaperonin
*	KT2440	3,440,348	0	T	G	46.3%	3.4 / 15.2	43	A19A (GCT→GCG)	CJ019_03266	hypothetical protein
*	KT2440	4,446,461	0	A	C	44.4%	28.1 / 12.6	45	E807A (GAG→GCG)	nicB	Nicotinate dehydrogenase subunit B
*	KT2440	3,626,106	0	T	G	40.0%	42.8 / 12.4	55	V174G (GTG→GGG)	hcf136_3	Ycf48‑like protein
*	KT2440	5,689,816	0	A	C	39.1%	34.0 / 14.5	66	S236R (AGC→CGC)	pyrC'	Dihydroorotase‑like protein
*	KT2440	3,586,408	0	C	A	38.2%	72.9 / 10.5	58	F129L (TTC→TTA)	CJ019_03399	hypothetical protein
*	KT2440	434,327	0	A	C	36.4%	64.0 / 10.9	45	H53P (CAC→CCC)	bioF	8‑amino‑7‑oxononanoate synthase
*	KT2440	2,578,050	0	A	C	36.1%	41.9 / 10.3	36	S168A (TCC→GCC)	folD_2	Bifunctional protein FolD protein
*	KT2440	5,384,276	0	C	G	36.0%	74.7 / 18.9	51	intergenic (+539/‑319)	CJ019_05064/CJ019_05067	putative protein/hypothetical protein
*	KT2440	2,752,369	0	G	C	34.3%	33.2 / 10.9	36	A523G (GCC→GGC)	CJ019_02595	hypothetical protein
*	KT2440	783,298	0	G	C	33.9%	100.4 / 11.1	56	P141R (CCG→CGG)	greB_1	Transcription elongation factor GreB
*	KT2440	3,138,367	0	T	G	33.3%	76.5 / 21.9	63	F240V (TTT→GTT)	rbsC_2	Ribose import permease protein RbsC
*	KT2440	5,689,824	0	T	G	31.4%	73.0 / 11.5	70	R238R (CGT→CGG)	pyrC'	Dihydroorotase‑like protein
*	KT2440	5,384,266	0	T	G	30.2%	87.0 / 11.0	53	intergenic (+529/‑329)	CJ019_05064/CJ019_05067	putative protein/hypothetical protein
*	KT2440	4,934,714	0	G	C	30.0%	63.0 / 12.2	40	A142P (GCC→CCC)	aar	L‑alanine/L‑glutamate racemase

Marginal new junction evidence (sorted from low to high skew)
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	KT2440	= 5982592	36 (0.450)	3 (0.040)	3/244	8.0	6.5%	intergenic (+51/‑638)	kefC_3/CJ019_05604	Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein
	?	KT2440	5982814 =	52 (0.690)					intergenic (+273/‑416)	kefC_3/CJ019_05604	Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein
*	?	KT2440	= 5074639	83 (1.030)	3 (0.040) +TCG	3/254	8.3	3.6%	coding (726/1236 nt)	CJ019_04774	Aspartate kinase
	?	KT2440	5074640 =	83 (1.030)					coding (725/1236 nt)	CJ019_04774	Aspartate kinase