breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,856,8860TG53.6% ‑4.2 / 12.5 29S297A (TCG→GCG) hdfR_4HTH‑type transcriptional regulator HdfR
*KT2440434,3730TG52.6% 1.5 / 14.5 19G68G (GGT→GGGbioF8‑amino‑7‑oxononanoate synthase
*KT24401,740,4070TG52.2% 0.2 / 11.0 23E53A (GAG→GCG) CJ019_01643hypothetical protein
*KT24401,524,7320TG44.4% 21.2 / 11.4 37Q67P (CAG→CCG) CJ019_01439hypothetical protein
*KT24402,094,8080AC43.4% 12.7 / 14.1 53L186R (CTG→CGG) CJ019_01995hypothetical protein
*KT2440916,6960TG43.1% 68.3 / 14.2 58Q516P (CAG→CCG) ltxBLeukotoxin export ATP‑binding protein LtxB
*KT24405,384,2650CG42.0% 53.9 / 23.2 51intergenic (+528/‑330)CJ019_05064/CJ019_05067putative protein/hypothetical protein
*KT24405,384,2760CG38.0% 47.6 / 32.0 52intergenic (+539/‑319)CJ019_05064/CJ019_05067putative protein/hypothetical protein
*KT24404,350,3600TG37.7% 71.3 / 11.8 53R28R (CGT→CGGCJ019_04090hypothetical protein
*KT24403,138,3670TG37.3% 70.0 / 24.0 67F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
*KT24403,586,4080CA37.1% 78.1 / 20.6 70F129L (TTC→TTACJ019_03399hypothetical protein
*KT24405,384,2730GT34.6% 56.7 / 16.2 52intergenic (+536/‑322)CJ019_05064/CJ019_05067putative protein/hypothetical protein
*KT24405,384,2860GT34.6% 61.0 / 17.1 52intergenic (+549/‑309)CJ019_05064/CJ019_05067putative protein/hypothetical protein
*KT24401,537,4150CG34.3% 49.8 / 18.4 36A388G (GCG→GGG) mdfAMultidrug transporter MdfA
*KT24401,537,4210TG31.4% 54.9 / 15.5 35V390G (GTG→GGG) mdfAMultidrug transporter MdfA
*KT24401,537,4120CG30.6% 58.9 / 16.0 36A387G (GCG→GGG) mdfAMultidrug transporter MdfA
*KT24402,113,8950TG30.4% 67.4 / 10.8 46R663R (CGT→CGGCJ019_02007hypothetical protein
*KT2440452,9400GC27.6% 91.0 / 11.0 61intergenic (‑36/+274)pqqB/CJ019_00410Coenzyme PQQ synthesis protein B/hypothetical protein
*KT24405,384,2890CG27.5% 84.6 / 11.3 52intergenic (+552/‑306)CJ019_05064/CJ019_05067putative protein/hypothetical protein
*KT24402,770,0920GC26.7% 132.1 / 21.5 76A157G (GCG→GGG) CJ019_02613hypothetical protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? KT2440 2264809 =44 (0.600)5 (0.070) 4/254 7.4 8.9% noncoding (37/77 nt)
noncoding (37/77 nt)
PP_RS08755
PP_RS08765
tRNA‑Asp
tRNA‑Asp
?KT2440 = 3134198 59 (0.830)coding (58/972 nt) CJ019_02952 hypothetical protein
* ? KT2440 1891946 =24 (0.330)3 (0.040) 3/254 8.0 6.8% noncoding (37/77 nt)
noncoding (37/77 nt)
PP_RS08755
PP_RS08765
tRNA‑Asp
tRNA‑Asp
?KT2440 = 3134198 59 (0.830)coding (58/972 nt) CJ019_02952 hypothetical protein
* ? KT2440 1891610 =20 (0.270)3 (0.040) 3/254 8.0 7.1% noncoding (37/77 nt)
noncoding (37/77 nt)
PP_RS08755
PP_RS08765
tRNA‑Asp
tRNA‑Asp
?KT2440 = 3134198 59 (0.830)coding (58/972 nt) CJ019_02952 hypothetical protein