breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 11,127 C→G 5.3% S77T (AGT→ACT)  yaaW ← UPF0174 family protein
RA 116,035 G→A 5.2% C355C (TGC→TGT hofB ← T2SE secretion family protein; P‑loop ATPase superfamily protein
RA 231,589 C→T 5.5% H156H (CAC→CAT yafD → endo/exonuclease/phosphatase family protein
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 286,206 G→C 5.9% G271A (GGC→GCC)  yagG → CP4‑6 prophage; putative sugar transporter
RA 347,188 G→A 5.7% A419V (GCG→GTG)  prpR ← propionate catabolism operon regulatory protein
RA 496,157 T→C 5.2% R346R (CGT→CGC htpG → protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase
RA 498,683 G→A 5.2% R210H (CGT→CAT)  hemH → ferrochelatase
RA 518,860 A→G 5.9% F97S (TTC→TCC)  ybbO ← putative oxidoreductase
RA 603,688 T→A 5.8% T326S (ACC→TCC)  ybdG ← mechanosensitive channel protein, miniconductance
RA 624,659 A→T 5.8% intergenic (‑149/‑226) fepB ← / → entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
RA 624,664 G→T 7.2% intergenic (‑154/‑221) fepB ← / → entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
RA 624,665 G→C 6.8% intergenic (‑155/‑220) fepB ← / → entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
RA 624,666 A→C 6.5% intergenic (‑156/‑219) fepB ← / → entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
RA 624,671 A→T 7.3% intergenic (‑161/‑214) fepB ← / → entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
RA 699,433 C→A 6.3% intergenic (‑256/+141) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 699,700 G→A 6.0% A209A (GCC→GCT umpH ← UMP phosphatase
RA 884,088 Δ1 bp 5.7% coding (416/1233 nt) mdfA → multidrug efflux system protein
RA 896,520 T→C 6.4% P129P (CCT→CCC potH → putrescine ABC transporter permease
RA 930,357 A→G 6.9% S202S (AGT→AGC cydD ← glutathione/cysteine ABC transporter export permease/ATPase
RA 936,594 C→A 5.1% A1124D (GCC→GAC)  ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 958,769 C→G 7.3% intergenic (+28/‑43) serC → / → aroA 3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase
RA 958,771 A→T 7.2% intergenic (+30/‑41) serC → / → aroA 3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase
RA 958,773 C→G 8.3% intergenic (+32/‑39) serC → / → aroA 3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase
RA 1,072,613 G→T 5.6% P84H (CCC→CAC)  rutB ← ureidoacrylate amidohydrolase
RA 1,085,667 T→C 5.1% Y226H (TAT→CAT)  phoH → ATP‑binding protein; putative PhoH family P‑loop ATPase
RA 1,087,337 G→A 5.8% R170R (CGC→CGT pgaC ← biofilm PGA synthase PgaCD, catalytic subunit; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA 1,134,222 G→A 6.4% G141S (GGC→AGC)  flgF → flagellar component of cell‑proximal portion of basal‑body rod
RA 1,146,137 A→G 6.1% S153S (AGT→AGC yceF ← m(7)GTP pyrophosphatase
RA 1,174,077 G→A 6.4% intergenic (‑113/+15) mfd ← / ← ycfT transcription‑repair coupling factor/inner membrane protein
RA 1,174,080 T→C 6.6% intergenic (‑116/+12) mfd ← / ← ycfT transcription‑repair coupling factor/inner membrane protein
RA 1,258,755 T→A 7.0% intergenic (‑242/‑36) pth ← / → ychH peptidyl‑tRNA hydrolase/DUF2583 family putative inner membrane protein
RA 1,286,965 A→G 10.4% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,286,983 A→G 8.2% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,328,724 A→T 6.9% M130K (ATG→AAG)  yciK ← putative EmrKY‑TolC system oxoacyl‑(acyl carrier protein) reductase
RA 1,394,083 C→T 6.3% T286M (ACG→ATG)  mppA → murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
RA 1,398,552 G→A 5.4% A24V (GCT→GTT)  uspE ← stress‑induced protein
RA 1,446,357:1 +T 5.5% intergenic (+151/+21) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB
RA 1,446,362 Δ1 bp 5.3% intergenic (+156/+16) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB
RA 1,455,598 C→T 5.3% G145G (GGC→GGT paaC → ring 1,2‑phenylacetyl‑CoA epoxidase subunit
RA 1,456,627 C→T 6.0% G66G (GGC→GGT paaE → ring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component
RA 1,460,981 C→A 5.7% A30D (GCT→GAT)  paaJ → 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
RA 1,464,897 G→T 6.6% E143* (GAA→TAA)  paaY → thioesterase required for phenylacetic acid degradation; trimeric; phenylacetate regulatory and detoxification protein; hexapeptide repeat protein
RA 1,563,432 T→A 5.3% I768F (ATC→TTC)  dosP ← oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
RA 1,567,466 G→C 5.5% intergenic (‑326/+38) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
RA 1,567,469 A→T 5.3% intergenic (‑329/+35) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
RA 1,567,471 A→C 7.4% intergenic (‑331/+33) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
RA 1,567,472 G→C 5.3% intergenic (‑332/+32) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
RA 1,592,022 A→G 6.7% I52T (ATC→ACC)  hipA ← inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase
RA 1,622,830 A→G 5.0% noncoding (85/98 nt) mgrR ← sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ‑regulated, Hfq‑dependent; affects sensitivity to antimicrobial peptides
RA 1,622,837 C→T 5.1% noncoding (78/98 nt) mgrR ← sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ‑regulated, Hfq‑dependent; affects sensitivity to antimicrobial peptides
RA 1,662,350 C→T 100% P599S (CCC→TCC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic
RA 1,806,319 C→T 100% intergenic (‑236/‑51) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
RA 1,807,094 T→C 100% V242A (GTG→GCG)  pfkB → 6‑phosphofructokinase II
RA 1,885,952 G→A 5.5% A36A (GCG→GCA yeaX → putative YeaWX dioxygenase beta subunit, reductase component
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,028,158 G→C 5.6% intergenic (‑141/+30) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein
RA 2,028,159 T→A 5.6% intergenic (‑142/+29) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein
RA 2,028,160 G→C 6.4% intergenic (‑143/+28) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein
RA 2,075,720 C→A 5.8% P314P (CCC→CCA yeeR → CP4‑44 prophage; putative membrane protein
RA 2,076,188 C→T 5.6% N470N (AAC→AAT yeeR → CP4‑44 prophage; putative membrane protein
RA 2,106,112 G→A 5.6% T372I (ACT→ATT)  wbbH ← O‑antigen polymerase
RA 2,134,710 T→C 5.2% N315D (AAC→GAC)  wzc ← colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,230,246 T→C 5.5% H47R (CAT→CGT)  dusC ← tRNA‑dihydrouridine synthase C
RA 2,260,232 C→T 6.4% L393L (CTG→CTA fruA ← fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
RA 2,282,830 T→C 7.1% intergenic (+29/+110) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,282,831 C→G 6.1% intergenic (+30/+109) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,282,832 C→G 6.9% intergenic (+31/+108) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,282,833 G→A 6.1% intergenic (+32/+107) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
RA 2,310,322 A→T 5.8% V28V (GTT→GTA) ‡ ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
RA 2,310,323 A→T 6.0% V28D (GTT→GAT) ‡ ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
RA 2,310,326 A→T 5.7% F27Y (TTC→TAC) ‡ ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
RA 2,310,327 A→T 5.3% F27I (TTC→ATC) ‡ ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
RA 2,339,980 G→A 5.1% P138P (CCG→CCA ubiG → bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase
RA 2,357,653 A→G 5.6% L250L (CTA→CTG yfaD → transposase_31 family protein
RA 2,383,956 A→T 5.4% intergenic (+32/+39) elaD → / ← yfbK protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
RA 2,431,978 T→C 5.3% K322R (AAA→AGA)  folC ← bifunctional folylpolyglutamate synthase/ dihydrofolate synthase
RA 2,577,513 G→A 5.9% P289S (CCG→TCG)  maeB ← malic enzyme: putative oxidoreductase/phosphotransacetylase
RA 2,591,970 A→T 5.0% T122S (ACG→TCG)  dapE → N‑succinyl‑diaminopimelate deacylase
RA 2,647,024 C→T 5.9% R101H (CGC→CAC)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
RA 2,665,193 T→A 5.1% Y268* (TAT→TAA yfhR → putative S9 family prolyl oligopeptidase
RA 2,734,830 G→A 5.1% Q69* (CAG→TAG)  yfiH ← UPF0124 family protein
RA 2,800,662 A→G 5.2% intergenic (+187/‑61) ygaM → / → nrdH putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
RA 2,884,577 T→C 5.3% R881R (AGA→AGG) ‡ ygcB ← Cascade complex anti‑viral R‑loop helicase‑annealase Cas3
RA 2,884,578 C→A 5.3% R881I (AGA→ATA) ‡ ygcB ← Cascade complex anti‑viral R‑loop helicase‑annealase Cas3
RA 2,884,579 T→G 5.3% R881R (AGA→CGA) ‡ ygcB ← Cascade complex anti‑viral R‑loop helicase‑annealase Cas3
RA 2,900,322 C→T 7.0% intergenic (‑49/‑270) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,900,324 G→T 6.6% intergenic (‑51/‑268) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,900,327 A→C 7.5% intergenic (‑54/‑265) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,936,203 C→T 5.1% A207V (GCG→GTG)  fucI → L‑fucose isomerase
RA 3,000,916 C→T 6.0% A191V (GCG→GTG)  xdhA → xanthine dehydrogenase, molybdenum binding subunit
RA 3,011,632 G→A 5.2% A58T (GCC→ACC)  yqeA → putative amino acid kinase
RA 3,052,025 C→A 5.5% S98I (AGC→ATC)  ubiI ← 2‑octaprenylphenol hydroxylase, FAD‑dependent
RA 3,125,414 Δ1 bp 6.4% coding (46/1224 nt) glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
RA 3,125,415 Δ1 bp 6.4% coding (45/1224 nt) glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
RA 3,228,476 C→T 5.3% L226F (CTT→TTT)  ygjJ → putative periplasmic protein
RA 3,331,276 G→C 6.0% A160G (GCT→GGT)  obgE ← GTPase involved in cell partioning and DNA repair
RA 3,351,482 G→A 5.5% A515V (GCG→GTG)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
RA 3,448,278 C→A 5.1% intergenic (‑129/+36) rplN ← / ← rpsQ 50S ribosomal subunit protein L14/30S ribosomal subunit protein S17
RA 3,495,131 T→C 5.7% V374A (GTG→GCG)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,576,161 G→C 5.6% Q301E (CAA→GAA)  gntU ← gluconate transporter, low affinity GNT 1 system
RA 3,750,144 A→T 5.0% H20L (CAT→CTT)  sgbE → L‑ribulose‑5‑phosphate 4‑epimerase
RA 3,781,132 C→G 6.1% intergenic (+115/‑83) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,138 A→C 8.1% intergenic (+121/‑77) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,830,727 C→T 6.1% Q91* (CAA→TAA)  yicH → putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,959,924 A→T 5.5% intergenic (‑111/+88) ppiC ← / ← yifN peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene
RA 3,959,925 A→T 5.4% intergenic (‑112/+87) ppiC ← / ← yifN peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene
RA 3,959,926 A→T 5.6% intergenic (‑113/+86) ppiC ← / ← yifN peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene
RA 3,978,113 G→A 5.1% E197K (GAG→AAG)  wecF → TDP‑Fuc4NAc:lipidIIFuc4NAc transferase
RA 4,081,323 C→T 5.2% A180T (GCC→ACC)  fdoI ← formate dehydrogenase‑O, cytochrome b556 subunit
RA 4,151,901 T→C 5.8% T400A (ACG→GCG)  ppc ← phosphoenolpyruvate carboxylase
RA 4,187,214 A→C 100% D622A (GAC→GCC)  rpoC → RNA polymerase, beta prime subunit
RA 4,250,156 C→T 5.0% T201T (ACC→ACT malM → maltose regulon periplasmic protein
RA 4,296,060 C→T 27.8% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,390,401 C→A 5.2% intergenic (‑41/+56) psd ← / ← rsgA phosphatidylserine decarboxylase/ribosome small subunit‑dependent GTPase A
RA 4,392,617 C→G 6.4% noncoding (35/76 nt) glyY → tRNA‑Gly
RA 4,520,500 Δ1 bp 5.9% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,520,504:1 +T 5.8% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,564,528 C→T 6.2% T54T (ACG→ACA yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423739–3424234 3424558–3424238 5–820 35 [32] [29] 36 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4107527 4108510 984 34 [32] [32] 33 [pfkA] [pfkA]
* * ÷ NC_000913 4144004 4147101 3098 33 [32] [31] 33 [pflD]–[pflC] [pflD],[pflC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 274620 =NA (NA)3 (0.040) 3/244 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 1207790 =40 (0.490)36 (0.510) 32/230 NT 51.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 33 (0.460)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780542 (0.520)37 (0.520) 34/230 NT 51.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 33 (0.460)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 2291337NA (NA)4 (0.050) 4/244 NT NA noncoding (185/199 nt) RIP160 (repetitive extragenic palindromic) element; contains 4 REP sequences and 1 IHF site RIP160 (repetitive extragenic palindromic) element; contains 4 REP sequences and 1 IHF site
?NC_000913 = 2291342 NA (NA)noncoding (190/199 nt) RIP160 (repetitive extragenic palindromic) element; contains 4 REP sequences and 1 IHF site RIP160 (repetitive extragenic palindromic) element; contains 4 REP sequences and 1 IHF site
* ? NC_000913 = 229669057 (0.700)4 (0.050) 4/252 NT 6.3% coding (335/663 nt) ccmB heme export ABC transporter permease
?NC_000913 = 2296727 65 (0.830)coding (298/663 nt) ccmB heme export ABC transporter permease
* ? NC_000913 3423318 =103 (1.270)7 (0.090) 5/244 NT 7.1% intergenic (+124/+105) yhdZ/rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
?NC_000913 3423341 = 87 (1.150)intergenic (+147/+82) yhdZ/rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
* ? NC_000913 3587430 =99 (1.220)5 (0.070) 4/242 NT 5.5% coding (684/744 nt) ugpQ glycerophosphodiester phosphodiesterase, cytosolic
?NC_000913 3587464 = 80 (1.070)coding (650/744 nt) ugpQ glycerophosphodiester phosphodiesterase, cytosolic