breseq version 0.28.1 revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 27 shown) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | DH1 | 936,169 | 0 | T | . | 39.6% | 109.0 / 79.9 | 54 | intergenic (‑13/+21) | ECDH1_RS04615/ECDH1_RS04620 | tRNA‑Met/tRNA‑Met |
* | DH1 | 936,297 | 0 | A | G | 36.5% | 140.2 / 102.4 | 102 | intergenic (‑31/+3) | ECDH1_RS04620/ECDH1_RS04625 | tRNA‑Met/tRNA‑Met |
* | DH1 | 936,293 | 0 | T | . | 33.8% | 143.5 / 85.3 | 74 | intergenic (‑27/+7) | ECDH1_RS04620/ECDH1_RS04625 | tRNA‑Met/tRNA‑Met |
* | DH1 | 3,448,526 | 0 | A | C | 30.9% | 413.2 / 65.9 | 243 | intergenic (‑86/+70) | malZ/proY | alpha‑glycosidase/proline‑specific permease ProY |
* | DH1 | 4,122,124 | 0 | C | A | 28.1% | 211.4 / 22.4 | 148 | intergenic (‑15/+22) | ECDH1_RS20430/ECDH1_RS20435 | tRNA‑Gly/tRNA‑Gly |
* | DH1 | 613,770 | 0 | C | A | 28.0% | 149.5 / 10.5 | 117 | intergenic (+631/+392) | garK/yhaC | glycerate 2‑kinase/pentapeptide repeats‑related protein |
* | DH1 | 2,591,513 | 0 | T | G | 28.0% | 102.7 / 28.8 | 140 | D86A (GAC→GCC) | ECDH1_RS12815 | hypothetical protein |
* | DH1 | 3,184,585 | 0 | C | A | 27.9% | 159.3 / 24.3 | 81 | intergenic (+8/‑30) | ECDH1_RS15795/ECDH1_RS15800 | tRNA‑Gln/tRNA‑TERM |
* | DH1 | 2,169,673 | 0 | A | T | 27.3% | 54.5 / 19.9 | 87 | R629R (CGT→CGA) | rsxC | electron transporter RsxC |
* | DH1 | 1,165,444 | 0 | A | C | 26.2% | 388.7 / 34.6 | 169 | T71P (ACG→CCG) | yfiF | methyltransferase |
* | DH1 | 3,448,523 | 0 | A | C | 25.6% | 502.4 / 47.7 | 243 | intergenic (‑83/+73) | malZ/proY | alpha‑glycosidase/proline‑specific permease ProY |
* | DH1 | 936,278 | 1 | . | T | 25.0% | 108.3 / 53.5 | 44 | intergenic (‑12/+22) | ECDH1_RS04620/ECDH1_RS04625 | tRNA‑Met/tRNA‑Met |
* | DH1 | 2,169,577 | 0 | T | G | 24.8% | 166.8 / 29.5 | 151 | R661R (CGA→CGC) | rsxC | electron transporter RsxC |
* | DH1 | 2,169,720 | 0 | C | G | 24.5% | 107.4 / 14.6 | 97 | E614Q (GAA→CAA) | rsxC | electron transporter RsxC |
* | DH1 | 3,447,403 | 0 | A | C | 24.1% | 410.5 / 39.5 | 209 | G346G (GGT→GGG) | malZ | alpha‑glycosidase |
* | DH1 | 1,519,307 | 0 | C | T | 24.0% | 533.9 / 147.9 | 276 | G254G (GGG→GGA) | yfaD | transposase |
* | DH1 | 613,597 | 0 | G | C | 23.9% | 224.4 / 26.1 | 152 | intergenic (+458/+565) | garK/yhaC | glycerate 2‑kinase/pentapeptide repeats‑related protein |
* | DH1 | 2,169,548 | 0 | G | T | 23.9% | 299.3 / 24.3 | 161 | P671Q (CCG→CAG) | rsxC | electron transporter RsxC |
* | DH1 | 1,065,605 | 0 | A | T | 23.1% | 267.3 / 69.5 | 159 | intergenic (+40/‑23) | ECDH1_RS05245/ECDH1_RS05250 | tRNA‑Arg/tRNA‑Arg |
* | DH1 | 1,647,682 | 0 | T | G | 22.8% | 218.1 / 21.7 | 148 | intergenic (+261/+112) | dusC/ECDH1_RS08050 | tRNA dihydrouridine synthase DusC/hypothetical protein |
Marginal new junction evidence (lowest skew 10 of 43 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | DH1 | = 2668875 | 210 (0.770) | 66 (0.270) | 58/268 | 9.2 | 28.0% | pseudogene (20/345 nt) | pinE | hypothetical protein |
? | DH1 | = 2670672 | 152 (0.620) | coding (306/789 nt) | stfP | integrase | |||||
* | ? | DH1 | 2668875 = | 182 (0.740) | 53 (0.220) | 44/268 | 12.8 | 22.7% | pseudogene (20/345 nt) | pinE | hypothetical protein |
? | DH1 | 2670674 = | NA (NA) | noncoding (1/242 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | = 1519281 | 197 (0.720) | 44 (0.170) +AACCTTCTAATT |
38/276 | 15.2 | 19.5% | coding (788/900 nt) | yfaD | transposase |
? | DH1 | 1519282 = | 197 (0.720) | coding (787/900 nt) | yfaD | transposase | |||||
* | ? | DH1 | 3183985 = | 248 (0.900) | 50 (0.200) +GATTGTTTCTTC |
31/276 | 17.7 | 24.3% | intergenic (+379/‑1) | asnB/ECDH1_RS15770 | asparagine synthetase B/tRNA‑Met |
? | DH1 | = 3184256 | 90 (0.330) | intergenic (+2/‑33) | ECDH1_RS15780/ECDH1_RS15785 | tRNA‑Gln/tRNA‑Gln | |||||
* | ? | DH1 | = 1355505 | 241 (0.880) | 42 (0.180) | 25/260 | 19.1 | 20.6% | noncoding (54/348 nt) | repeat_region | repeat region |
? | DH1 | 3099693 = | 116 (0.490) | noncoding (58/76 nt) | ECDH1_RS15360 | tRNA‑Lys | |||||
* | ? | DH1 | = 2590941 | 228 (0.830) | 33 (0.140) | 20/260 | 21.5 | 19.7% | intergenic (+1/‑209) | ECDH1_RS12805/ECDH1_RS12810 | tRNA‑Tyr/tRNA‑Tyr |
? | DH1 | 2591414 = | 71 (0.300) | noncoding (199/555 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | 1355966 = | 124 (0.450) | 50 (0.190) +CTTC |
21/292 | 23.1 | 25.2% | noncoding (76/76 nt) | ECDH1_RS06685 | tRNA‑Val |
? | DH1 | = 3099750 | 181 (0.660) | noncoding (1/76 nt) | ECDH1_RS15360 | tRNA‑Lys | |||||
* | ? | DH1 | 1355966 = | 124 (0.450) | 38 (0.140) +CTTC |
20/292 | 23.6 | 14.4% | noncoding (76/76 nt) | ECDH1_RS06685 | tRNA‑Val |
? | DH1 | = 3100565 | 340 (1.240) | noncoding (1/76 nt) | ECDH1_RS15385 | tRNA‑Lys | |||||
* | ? | DH1 | 1355966 = | 124 (0.450) | 100 (0.370) +CTTC |
20/292 | 23.6 | 45.3% | noncoding (76/76 nt) | ECDH1_RS06685 | tRNA‑Val |
? | DH1 | = 3099542 | NA (NA) | noncoding (268/268 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | 1355844 = | 72 (0.260) | 48 (0.180) +C |
20/298 | 24.0 | 27.7% | intergenic (‑45/+2) | ECDH1_RS06675/ECDH1_RS06680 | tRNA‑Val/tRNA‑Val |
? | DH1 | = 3099750 | 180 (0.660) | noncoding (1/76 nt) | ECDH1_RS15360 | tRNA‑Lys |