breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 27 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,1690T.39.6% 109.0 / 79.9 54intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH1936,2970AG36.5% 140.2 / 102.4 102intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH1936,2930T.33.8% 143.5 / 85.3 74intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH13,448,5260AC30.9% 413.2 / 65.9 243intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH14,122,1240CA28.1% 211.4 / 22.4 148intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
*DH1613,7700CA28.0% 149.5 / 10.5 117intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,591,5130TG28.0% 102.7 / 28.8 140D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
*DH13,184,5850CA27.9% 159.3 / 24.3 81intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
*DH12,169,6730AT27.3% 54.5 / 19.9 87R629R (CGT→CGArsxCelectron transporter RsxC
*DH11,165,4440AC26.2% 388.7 / 34.6 169T71P (ACG→CCG) yfiFmethyltransferase
*DH13,448,5230AC25.6% 502.4 / 47.7 243intergenic (‑83/+73)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH1936,2781.T25.0% 108.3 / 53.5 44intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,169,5770TG24.8% 166.8 / 29.5 151R661R (CGA→CGCrsxCelectron transporter RsxC
*DH12,169,7200CG24.5% 107.4 / 14.6 97E614Q (GAA→CAA) rsxCelectron transporter RsxC
*DH13,447,4030AC24.1% 410.5 / 39.5 209G346G (GGT→GGGmalZalpha‑glycosidase
*DH11,519,3070CT24.0% 533.9 / 147.9 276G254G (GGG→GGAyfaDtransposase
*DH1613,5970GC23.9% 224.4 / 26.1 152intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,169,5480GT23.9% 299.3 / 24.3 161P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH11,065,6050AT23.1% 267.3 / 69.5 159intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH11,647,6820TG22.8% 218.1 / 21.7 148intergenic (+261/+112)dusC/ECDH1_RS08050tRNA dihydrouridine synthase DusC/hypothetical protein

Marginal new junction evidence (lowest skew 10 of 43 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 2668875210 (0.770)66 (0.270) 58/268 9.2 28.0% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 = 2670672 152 (0.620)coding (306/789 nt) stfP integrase
* ? DH1 2668875 =182 (0.740)53 (0.220) 44/268 12.8 22.7% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 2670674 = NA (NA)noncoding (1/242 nt) repeat_region repeat region
* ? DH1 = 1519281197 (0.720)44 (0.170)
+AACCTTCTAATT
38/276 15.2 19.5% coding (788/900 nt) yfaD transposase
?DH1 1519282 = 197 (0.720)coding (787/900 nt) yfaD transposase
* ? DH1 3183985 =248 (0.900)50 (0.200)
+GATTGTTTCTTC
31/276 17.7 24.3% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 90 (0.330)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 = 1355505241 (0.880)42 (0.180) 25/260 19.1 20.6% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 116 (0.490)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 2590941228 (0.830)33 (0.140) 20/260 21.5 19.7% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 71 (0.300)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 1355966 =124 (0.450)50 (0.190)
+CTTC
21/292 23.1 25.2% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 181 (0.660)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =124 (0.450)38 (0.140)
+CTTC
20/292 23.6 14.4% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3100565 340 (1.240)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355966 =124 (0.450)100 (0.370)
+CTTC
20/292 23.6 45.3% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 1355844 =72 (0.260)48 (0.180)
+C
20/298 24.0 27.7% intergenic (‑45/+2) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 180 (0.660)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys