breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 43 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2930T.43.4% 201.0 / 195.8 118intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH1936,2970AG41.5% 145.8 / 178.8 153intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH1936,1690T.40.0% 128.6 / 91.2 60intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH1936,2781.T38.6% 112.7 / 145.3 70intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH14,122,1240CA32.0% 284.9 / 19.5 197intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
*DH1613,5970GC31.0% 240.4 / 50.9 209intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH1613,7700CA29.8% 185.6 / 18.4 145intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,326,2870GT29.2% 650.3 / 104.0 360intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH12,169,5480GT28.6% 280.3 / 44.5 156P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH11,065,6050AT28.5% 230.0 / 93.7 158intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH13,448,5260AC27.9% 541.6 / 82.8 318intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH12,169,5770TG27.7% 167.5 / 35.0 147R661R (CGA→CGCrsxCelectron transporter RsxC
*DH13,447,4030AC25.9% 523.6 / 66.5 287G346G (GGT→GGGmalZalpha‑glycosidase
*DH12,591,5130TG24.9% 179.5 / 37.1 209D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
*DH1613,6130TG24.5% 160.4 / 26.9 168intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH1613,7760TA24.5% 249.4 / 29.2 157intergenic (+637/+386)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH11,065,6140AG24.3% 251.1 / 93.7 168intergenic (+49/‑14)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH11,065,6180AG24.2% 264.2 / 98.4 174intergenic (+53/‑10)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH12,169,7810GT22.8% 333.5 / 41.3 175A593A (GCC→GCArsxCelectron transporter RsxC
*DH11,165,4440AC22.7% 646.0 / 48.2 279T71P (ACG→CCG) yfiFmethyltransferase

Marginal new junction evidence (lowest skew 10 of 39 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 2668875241 (0.640)97 (0.290) 79/268 10.1 31.1% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 = 2670672 215 (0.640)coding (306/789 nt) stfP integrase
* ? DH1 2668875 =215 (0.640)83 (0.250) 66/268 13.2 28.0% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 2670674 = NA (NA)noncoding (1/242 nt) repeat_region repeat region
* ? DH1 = 1519086232 (0.620)37 (0.140) 32/220 20.3 19.6% coding (71/186 nt) ECDH1_RS07485 hypothetical protein
?DH1 1519282 = 133 (0.490)coding (787/900 nt) yfaD transposase
* ? DH1 = 2590941314 (0.840)57 (0.180) 36/260 22.3 23.5% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 100 (0.310)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 1355843 =69 (0.180)155 (0.420)
+C
44/298 22.3 50.9% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 232 (0.620)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 1355505358 (0.960)67 (0.210) 35/260 22.7 21.9% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 168 (0.520)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =130 (0.350)118 (0.320)
+CTTC
41/292 23.0 40.1% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 232 (0.620)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355843 =69 (0.180)118 (0.320)
+C
42/298 23.1 33.0% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3100565 414 (1.110)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355843 =69 (0.180)207 (0.560)
+C
42/298 23.1 75.1% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 1355966 =130 (0.350)85 (0.230)
+CTTC
40/292 23.4 24.3% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3100565 414 (1.110)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys