breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 39 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,1690T.51.6% 105.3 / 169.1 63intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH1936,2930T.38.9% 139.9 / 108.8 79intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH13,448,5260AC33.0% 451.2 / 97.6 283intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH1613,7700CA32.8% 149.7 / 31.9 148intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH1936,2781.T32.7% 109.2 / 94.6 55intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH1936,2970AG31.7% 204.6 / 108.3 125intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH13,184,5850CA30.8% 164.3 / 37.4 107intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
*DH12,169,5480GT30.8% 286.6 / 45.3 157P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH1613,5970GC30.4% 168.5 / 43.2 167intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,169,5770TG30.2% 167.4 / 36.5 144R661R (CGA→CGCrsxCelectron transporter RsxC
*DH11,065,6180AG28.0% 167.7 / 81.1 117intergenic (+53/‑10)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH12,169,7200CG27.5% 128.4 / 39.4 125E614Q (GAA→CAA) rsxCelectron transporter RsxC
*DH12,591,5130TG27.5% 124.3 / 33.7 170D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
*DH13,906,8370CG27.0% 106.1 / 10.8 70intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH13,448,5200AC26.0% 591.4 / 52.2 283intergenic (‑80/+76)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH12,591,4410GC25.6% 187.6 / 23.2 133intergenic (+207/+53)ECDH1_RS12810/ECDH1_RS12815tRNA‑Tyr/hypothetical protein
*DH14,122,1240CA25.3% 267.8 / 11.5 175intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
*DH13,448,5230AC25.1% 584.5 / 48.4 283intergenic (‑83/+73)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH13,447,4030AC25.0% 497.7 / 52.0 252G346G (GGT→GGGmalZalpha‑glycosidase
*DH11,647,6690AC23.7% 180.8 / 15.7 150intergenic (+248/+125)dusC/ECDH1_RS08050tRNA dihydrouridine synthase DusC/hypothetical protein

Marginal new junction evidence (lowest skew 10 of 31 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 1355966 =130 (0.400)97 (0.300)
+CTTC
42/292 23.4 36.8% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 213 (0.650)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =130 (0.400)70 (0.220)
+CTTC
39/292 24.6 21.7% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3100565 388 (1.180)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355966 =130 (0.400)144 (0.450)
+CTTC
39/292 24.6 53.2% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 2668875 =270 (0.920)36 (0.120) 31/268 26.1 11.8% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 2670674 = NA (NA)noncoding (1/242 nt) repeat_region repeat region
* ? DH1 = 1519281216 (0.660)46 (0.150)
+AACCTTCTAATT
30/276 27.4 18.8% coding (788/900 nt) yfaD transposase
?DH1 1519282 = 216 (0.660)coding (787/900 nt) yfaD transposase
* ? DH1 = 1355505316 (0.960)50 (0.180) 27/260 27.4 19.1% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 151 (0.530)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355844 =82 (0.250)66 (0.200)
+C
32/298 28.4 31.1% intergenic (‑45/+2) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 212 (0.650)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =130 (0.400)88 (0.270)
+CTC
31/294 28.6 34.4% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 213 (0.650)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 3099328291 (0.890)41 (0.140) 24/260 29.1 16.9% noncoding (54/268 nt) repeat_region repeat region
?DH1 3099693 = 151 (0.530)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 1065291317 (0.970)35 (0.120) 23/260 29.7 12.9% intergenic (+1/‑198) ECDH1_RS05240/ECDH1_RS05245 tRNA‑Arg/tRNA‑Arg
?DH1 1065841 = 197 (0.690)intergenic (+137/‑62) ECDH1_RS05250/ECDH1_RS05255 tRNA‑Arg/tRNA‑Arg