breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 42 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,428,5200AG53.7% 118.1 / inf 377M36T (ATG→ACG) ubiX3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase
*NC_0009134,186,4250CT51.8% 57.9 / inf 508P359L (CCA→CTA) rpoCRNA polymerase, beta prime subunit
*NC_0009132,414,4610CT45.6% 152.2 / inf 461T331I (ACT→ATT) ackAacetate kinase A and propionate kinase 2
*NC_0009132,947,5910A.44.4% 176.3 / 161.3 90intergenic (+18/‑16)metW/metVtRNA‑Met/tRNA‑Met
*NC_0009134,188,5720CA44.2% 344.3 / inf 487R1075S (CGT→AGT) rpoCRNA polymerase, beta prime subunit
*NC_0009133,368,2140CT42.6% 292.9 / inf 531Q130* (CAG→TAG) yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
*NC_0009131,708,9010AC31.6% 255.8 / 75.7 241R661R (CGA→CGCrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_000913422,4290TG30.9% 691.9 / 122.2 413intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_0009134,392,4570GT28.2% 356.8 / 29.4 259intergenic (+22/‑15)glyV/glyXtRNA‑Gly/tRNA‑Gly
*NC_0009131,552,1910CA28.1% 819.1 / 107.6 437intergenic (+200/+207)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
*NC_0009131,708,7580GC28.0% 127.0 / 37.7 129E614Q (GAA→CAA) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009131,708,8050TA26.3% 122.3 / 37.3 145R629R (CGT→CGArsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009132,818,1580TA25.1% 331.2 / 107.2 200intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg
*NC_0009131,708,8800CA24.8% 330.7 / 35.6 202A654A (GCC→GCArsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009133,269,9940GT24.4% 303.4 / 17.6 212intergenic (+392/+631)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_0009132,818,1450TC24.3% 343.0 / 126.7 220intergenic (‑10/+53)argZ/argYtRNA‑Arg/tRNA‑Arg
*NC_000913422,4320TG24.3% 868.6 / 71.6 413intergenic (+73/‑86)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_0009133,270,1670CG24.0% 358.6 / 44.8 262intergenic (+565/+458)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_0009134,296,0600CT24.0% 882.5 / inf 630intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_000913422,4350TG23.4% 901.3 / 68.4 413intergenic (+76/‑83)proY/malZproline‑specific permease/maltodextrin glucosidase

Marginal new junction evidence (lowest skew 10 of 41 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1770322245 (0.530)327 (0.710) 146/300 3.8 57.2% intergenic (+136/‑293) ydiK/ydiL UPF0118 family inner membrane protein/putative HTH domain DNA‑binding protein
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 1770319 =248 (0.540)308 (0.670) 136/300 4.9 55.4% intergenic (+133/‑296) ydiK/ydiL UPF0118 family inner membrane protein/putative HTH domain DNA‑binding protein
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 1907451257 (0.560)262 (0.570) 134/300 5.1 50.5% coding (141/144 nt) yobF DUF2527 family heat‑induced protein
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 3815858269 (0.580)161 (0.370) 120/280 5.7 40.2% pseudogene (24/48 nt) rph ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
?NC_000913 3815941 = 229 (0.530)pseudogene (609/669 nt) rph ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
* ? NC_000913 1907448 =228 (0.510)254 (0.560) 120/292 6.4 52.7% coding (144/144 nt) yobF DUF2527 family heat‑induced protein
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 1287084142 (0.310)85 (0.210) 52/260 18.0 29.7% intergenic (+558/+3) narI/tpr nitrate reductase 1, gamma (cytochrome b(NR)) subunit/protamine‑like protein
?NC_000913 1287557 = 279 (0.700)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 781446258 (0.560)80 (0.200) 47/260 19.6 21.9% intergenic (+2/‑131) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 2521487 = 347 (0.870)intergenic (+159/+106) lysV/xapR tRNA‑Lys/transcriptional activator of xapAB
* ? NC_000913 781577 =324 (0.700)216 (0.480)
+GAAG
53/292 20.1 47.0% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 177 (0.380)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 780554 =519 (1.120)117 (0.260)
+GAAG
52/292 20.4 25.7% noncoding (1/76 nt) lysT tRNA‑Lys
?NC_000913 = 2521006 177 (0.380)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 781369 =267 (0.580)145 (0.320)
+GAAG
51/292 20.7 40.2% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 177 (0.380)noncoding (76/76 nt) valU tRNA‑Val