breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 124,241 C→T Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 461,007 T→G I707S (ATC→AGC)  lon → Lon protease
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 754,446 A→G L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,368,101 T→C A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,661,821 C→G C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,634 A→C T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,473,614 C→T W156* (TGG→TGA rpsG ← 30S ribosomal subunit protein S7
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp [rph] [rph]
JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 365876 366444 569 15 [14] [14] 15 [lacZ]–[lacI] [lacZ],[lacI]
* * ÷ NC_000913 3423752–3424530 3424530 1–779 16 [14] [14] 15 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 566777 =NA (NA)1275 (30.790) 248/282 -0.0 97.6% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 31 (0.740)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 1207790 =0 (0.000)26 (0.700) 25/252 0.4 100% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 0 (0.000)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078050 (0.000)28 (0.760) 26/252 0.3 100% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 0 (0.000)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment