breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 481,404 C→A Q1000H (CAG→CAT acrB ← multidrug efflux pump RND permease AcrB
JC JC 486,025 IS1 (–) +9 bp coding (265‑273/648 nt) acrR → DNA‑binding transcriptional repressor AcrR
RA 803,662 C→A L54I (CTT→ATT)  ybhJ → putative hydratase YbhJ
JC JC 883,553 IS30 (+) +2 bp intergenic (‑165/‑119) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 883,661 C→T intergenic (‑273/‑12) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
MC JC 1,619,100 Δ20 bp intergenic (‑192/‑1) marC ← / → marR inner membrane protein MarC/DNA‑binding transcriptional repressor MarR
MC JC 1,720,887 Δ17 bp intergenic (‑63/‑122) slyA ← / → ydhI DNA‑binding transcriptional dual regulator SlyA/DUF1656 domain‑containing protein YdhI
RA 1,905,761 G→A G25D (GGT→GAT)  mntP → Mn(2(+)) exporter
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp [rph] [rph]
MC JC 4,185,956 Δ36 bp coding (607‑642/4224 nt) rpoC → RNA polymerase subunit beta'
RA 4,390,754 G→T A252A (GCC→GCA rsgA ← ribosome small subunit‑dependent GTPase A

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423713–3424234 3424591–3424238 5–879 7 [5] [5] 7 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4296116–4296378 4296398 21–283 6 [5] [4] 6 gltP/yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =15 (0.380)25 (0.840) 17/96 0.2 55.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 29 (0.970)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780515 (0.380)26 (0.870) 10/96 0.7 56.4% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 29 (0.970)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)44 (1.180) 24/120 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein