breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 315,158 C→A pseudogene (802/888 nt) eaeH → putative porin domain‑containing protein EaeH
RA 416,770 C→A T61T (ACG→ACT sbcD ← ATP dependent, structure specific DNA nuclease ‑ SbcD subunit
RA 437,162 T→G *325S (TAA→TCA)  yajO ← 1‑deoxyxylulose‑5‑phosphate synthase YajO
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,058,863 C→A L260L (CTC→CTA torC → cytochrome c menaquinol dehydrogenase TorC
RA 1,201,284 T→A T33S (ACC→TCC)  ymfH ← e14 prophage; protein YmfH
RA 1,354,821 G→T A217E (GCG→GAG)  sapB ← putrescine ABC exporter membrane subunit SapB
RA 1,581,168 G→T P453T (CCA→ACA)  ydeN ← putative sulfatase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,800,810 C→A L587F (TTG→TTT thrS ← threonine‑‑tRNA ligase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,029,333 G→T L12I (CTC→ATC)  yedI ← conserved inner membrane protein YedI
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,324,606 G→T M166I (ATG→ATT atoA → acetyl‑CoA:acetoacetyl‑CoA transferase subunit beta
RA 2,573,300 T→G D69A (GAT→GCT)  eutD ← phosphate acetyltransferase EutD
RA 2,986,824 G→T K326N (AAG→AAT yqeG → putative transporter YqeG
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,453,155 G→T P39Q (CCG→CAG)  rpsJ ← 30S ribosomal subunit protein S10
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,653,361 C→A pseudogene (126/478 nt) yhiS → putative uncharacterized protein YhiS
RA 3,804,229 A→G S296S (AGT→AGC waaS ← lipopolysaccharide core biosynthesis protein
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,196,475 C→A E112* (GAA→TAA)  rsd ← regulator of sigma D
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,530,005 C→A G80V (GGC→GTC)  sgcC ← putative PTS enzyme IIC component SgcC
RA 4,547,373 C→A L760M (CTG→ATG)  fimD → type I fimbriae usher protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2518081 2518194 114 23 [22] [22] 25 [alaX]–[alaW] [alaX],[alaW]
* * ÷ NC_000913 3102960 3104078 1119 23 [22] [22] 24 mutY mutY
* * ÷ NC_000913 3423770–3424234 3424527–3424239 6–758 24 [18] [22] 23 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =81 (1.040)22 (0.320) 17/246 2.6 24.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 68 (0.990)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780581 (1.040)21 (0.310) 19/246 2.3 23.1% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 68 (0.990)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)82 (1.080) 57/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =92 (1.180)108 (1.400) 64/276 0.1 70.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)87 (1.120) 59/278 0.1 96.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA