breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 149,470 G→T G44G (GGC→GGA panB ← 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 309,451 C→A P192P (CCG→CCT ecpB ← putative fimbrial chaperone EcpB
RA 435,206 C→A Q24K (CAG→AAG)  nusB → transcription antitermination protein NusB
RA 643,960 C→A R3I (AGA→ATA)  rnk ← nucleoside diphosphate kinase regulator
RA 877,023 G→T P338Q (CCG→CAG)  rimO ← ribosomal protein S12 methylthiotransferase RimO
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
JC JC 1,092,683 IS5 (–) +4 bp intergenic (‑394/‑190) pgaA ← / → dgcT partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine outer membrane porin/putative diguanylate cyclase DgcT
RA 1,660,686 G→T R44L (CGG→CTG)  ynfF → putative selenate reductase YnfF
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,715,011 C→A intergenic (+29/+15) gstA → / ← pdxY glutathione S‑transferase GstA/pyridoxal kinase 2
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,039,191 C→A T79N (ACT→AAT)  yedX → hydroxyisourate hydrolase/transthyretin‑related protein
RA 2,068,083 G→T intergenic (‑762/‑552) insH‑6 ← / → yoeA CP4‑44 prophage; IS5 transposase and trans‑activator/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA
RA 2,078,265 C→A intergenic (+131/‑310) yeeW → / → yoeF CP4‑44 prophage; putative uncharacterized protein YeeW/CP4‑44 prophage; putative uncharacterized protein YoeF
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,560,093 G→T intergenic (+27/‑164) intZ → / → yffL CPZ‑55 prophage; putative phage integrase IntZ/CPZ‑55 prophage; uncharacterized protein YffL
RA 2,561,159 C→A intergenic (+261/‑209) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein YffL/CPZ‑55 prophage; uncharacterized protein YffM
RA 2,610,205 G→T A14A (GCG→GCT hyfH → hydrogenase 4 component H
RA 3,270,858 G→T P305T (CCG→ACG)  garK ← glycerate 2‑kinase 1
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,416,802 G→T L590F (TTG→TTT acrF → multidrug efflux pump RND permease AcrF
RA 3,459,294 A→G E319G (GAA→GGA)  gspE → Type II secretion system protein GspE
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,834,980 G→T A317D (GCC→GAC)  yicJ ← putative xyloside transporter YicJ
RA 3,963,226 G→T A335D (GCT→GAT)  gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 4,135,618 T→G L595R (CTG→CGG)  katG → hydroperoxidase I
RA 4,147,953 C→A G122V (GGT→GTT)  yijO ← putative DNA‑binding transcriptional regulator YijO
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,529,836 G→T T136T (ACC→ACA sgcC ← putative PTS enzyme IIC component SgcC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102965 3104047 1083 3 [2] [2] 3 [mutY] [mutY]
* * ÷ NC_000913 3423264 3423353 90 3 [2] [2] 3 yhdZ/rrfF putative ABC transporter ATP‑binding subunit YhdZ/5S ribosomal RNA
* * ÷ NC_000913 3423791–3424233 3424525–3424238 6–735 4 [2] [2] 8 [rrlD] [rrlD]
* * ÷ NC_000913 3802382 3802455 74 3 [2] [1] 4 waaO UDP‑D‑glucose:(glucosyl)LPS alpha‑1,3‑glucosyltransferase
* * ÷ NC_000913 3802700 3802788 89 4 [2] [2] 3 waaO UDP‑D‑glucose:(glucosyl)LPS alpha‑1,3‑glucosyltransferase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =37 (1.520)9 (0.420) 8/244 1.1 24.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 22 (1.030)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)40 (1.700) 31/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =23 (0.950)30 (1.240) 21/274 0.2 71.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)26 (1.070) 25/276 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA