breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 794,929 G→T intergenic (+7/‑160) acrZ → / → modA multidrug efflux pump accessory protein AcrZ/molybdate ABC transporter periplasmic binding protein
RA 797,443 G→T R297L (CGG→CTG)  modC → molybdate ABC transporter ATP binding subunit
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,233,327 G→T A464E (GCG→GAG)  nhaB ← Na(+):H(+) antiporter NhaB
RA 1,253,440 C→A S838I (AGC→ATC)  ycgV ← putative autotransporter adhesin YcgV
RA 1,390,202 C→A G133C (GGC→TGC)  mpaA ← murein tripeptide amidase A
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,067,274 T→C Q16Q (CAA→CAG insH‑6 ← CP4‑44 prophage; IS5 transposase and trans‑activator
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,344,166 G→T R2R (CGG→AGG)  yfaL ← putative autotransporter adhesin YfaL
RA 2,456,193 T→C intergenic (‑547/+134) yfcV ← / ← sixA putative fimbrial protein YfcV/phosphohistidine phosphatase
RA 2,598,092 C→A A258A (GCG→GCT bamC ← outer membrane protein assembly factor BamC
RA 3,053,387 G→T G44G (GGC→GGA ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase
RA 3,191,032 C→T T134I (ACC→ATC)  yqiI → putative fimbrial protein YqiI
RA 3,224,019 G→T A463S (GCG→TCG)  ebgA → evolved beta‑D‑galactosidase subunit alpha
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,634,831 G→T intergenic (‑373/‑10) yhiL ← / → yhiM putative uncharacterized protein YhiL/inner membrane protein with a role in acid resistance
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,049,431 T→C R822R (CGT→CGC polA → DNA polymerase I
RA 4,221,839 C→A G217W (GGG→TGG)  arpA ← regulator of acetyl CoA synthetase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,316,959 C→A S87I (AGC→ATC)  phnM ← RPnTP hydrolase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102957 3104048 1092 24 [22] [22] 24 [mutY] [mutY]
* * ÷ NC_000913 3423765–3424234 3424530–3424239 6–766 25 [21] [21] 24 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =80 (0.800)38 (0.430) 31/244 1.3 30.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 101 (1.140)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 1287362 =75 (0.750)53 (0.620) 37/236 0.8 38.5% intergenic (‑34/+176) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
?NC_000913 = 1287536 105 (1.230)intergenic (‑208/+2) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
* ? NC_000913 = 12994980 (0.000)145 (1.490) 78/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =161 (1.610)196 (1.980) 85/274 0.0 70.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)103 (1.030) 58/276 0.3 92.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 8 (0.080)coding (3907/4134 nt) rhsA rhs element protein RhsA