breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 228,975 T→G noncoding (48/77 nt) aspU → tRNA‑Asp
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC 279,931 Δ11,471 bp between IS1 yagBinsA‑3 yagB, yagA, yagE, yagF, yagG, yagH, xynR, argF, ykgS, insB‑3, insA‑3
RA 554,283 C→G G52A (GGC→GCC)  ppiB ← peptidyl‑prolyl cis‑trans isomerase B
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,095,929 C→T G29G (GGC→GGT ycdU → uncharacterized protein YcdU
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
MC JC 1,362,466 Δ19 bp [puuR] [puuR]
RA 1,398,849 G→T V226V (GTC→GTA fnr ← DNA‑binding transcriptional dual regulator FNR
RA 1,423,998 T→G F73V (TTT→GTT)  trkG → Rac prophage; K(+) transporter TrkG
RA 1,579,069 +T coding (274/1686 nt) yddA ← ABC transporter family protein YddA
RA 1,584,502 T→C D662G (GAT→GGT)  ydeP ← putative oxidoreductase YdeP
RA 1,602,087 C→A L200M (CTG→ATG)  lsrA → Autoinducer‑2 ABC transporter ATP binding subunit
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,788,325 G→T intergenic (+47/‑110) ppsR → / → aroH posphoenolpyruvate synthetase regulatory protein/3‑deoxy‑7‑phosphoheptulonate synthase, Trp‑sensitive
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,911,663 A→T intergenic (+14/+32) yebQ → / ← htpX putative transporter YebQ/zinc dependent endoprotease
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,337,170 C→A G751C (GGT→TGT)  gyrA ← DNA gyrase subunit A
RA 2,468,612 G→T T133T (ACG→ACT yfdH → CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,513,527 T→G I38L (ATT→CTT)  trpS ← tryptophan‑‑tRNA ligase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,656,250 C→A A15S (GCT→TCT)  hdeB ← periplasmic acid stress chaperone
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,120,807 T→C K314R (AAA→AGA)  hslU ← ATPase component of the HslVU protease
RA 4,154,941 C→A intergenic (‑94/‑60) argE ← / → argC acetylornithine deacetylase/N‑acetylglutamylphosphate reductase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,315,520 G→T R7S (CGC→AGC)  phnO ← aminoalkylphosphonate N‑acetyltransferase
RA 4,495,163 G→A A181T (GCC→ACC)  idnK → D‑gluconate kinase, thermosensitive

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196292 1203725 7434 7 [4] [4] 5 [icd]–[ymfL] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,[ymfL]
* * ÷ NC_000913 1203735 1211419 7685 5 [4] [4] 5 [ymfL]–[icdC] [ymfL],ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102970 3104081 1112 5 [4] [4] 6 mutY mutY
* * ÷ NC_000913 3423770–3424232 3424523–3424242 11–754 5 [2] [4] 5 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)43 (1.390) 36/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =72 (2.260)52 (1.650) 32/274 0.1 58.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)40 (1.260) 36/276 0.0 90.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.130)coding (3907/4134 nt) rhsA rhs element protein RhsA